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IGF-1 induces rat glomerular mesangial cells to accumulate
IGF-1 induces rat glomerular mesangial cells to accumulate

... of lipid accumulation on IGFBP-5-mediated migration, MC propagated with and without IGF-1 (100 nM) were incubated in standard medium or loaded with cholesteryl ester (2.5 ␮g/ml) or linoleic and oleic acids conjugated to albumin (9.3 ␮g/ml, Sigma) for 7 days. Migration was measured as described elsew ...
WGEP crawford boyer martins
WGEP crawford boyer martins

... extra few feet “just to be safe” and started adjusting the length appropriately. (pg. 398-399) ...
Lysine Acetylation - Regulator of Diverse Cellular Processes
Lysine Acetylation - Regulator of Diverse Cellular Processes

... and tumor suppressor P53 were shown to be lysine acetylated2-4. With the development of pan-acetyl-lysine antibodies and their use in mass spectrometry analyses, large scale proteomic studies of lysine acetylation sites revealed a previously unappreciated number of acetylated proteins. The first of ...
Truncated products of the vestigial proliferation gene
Truncated products of the vestigial proliferation gene

... disc integrating developmental cues by its two enhancers, the `boundary' and the `quadrant' enhancers. Ectopic expression of vg in all imaginal discs leads to ectopic proliferation of wing tissue.8 All vg mutants characterized so far have been considered to be hypomorph with a wing phentoype due to ...
A Hierarchy of Regulatory Genes Controls a Larva-to-Adult
A Hierarchy of Regulatory Genes Controls a Larva-to-Adult

... examined. For these experiments, mutations were used that had previously been determined to cause loss or gain of gene function (Ambros and Horvitz, 1984, 1987; see also Experimental Procedures). Apparent null alleles of h-14, /in-28, and /in-29 are fully recessive and were identified as mutations t ...
Identification and characterization of subpopulations
Identification and characterization of subpopulations

... distinguish the epiblast and PrE from the ICM by Rex1 expression. However, there have been no previous investigations to determine whether these genes are differentially expressed in each ES cell or whether each ES cell has a different pluripotent character. It is well known that ES cells can mimic ...
Inefficient response of T lymphocytes to
Inefficient response of T lymphocytes to

... To test whether antigenic peptides derived from endogenous GPIanchored proteins are presented on MHC class II molecules and if so whether the presentation is dependent on GPI anchoring, we used the GPI-anchored form of OVA as a model antigen. We isolated CD4⫹ T cells from lymph nodes of OTII mice th ...
Ty Jacobs` Bio Study Guide
Ty Jacobs` Bio Study Guide

...  Water readily dissolves ionic and polar solvents.  Many organic molecules are either ionic or polar (i.e. proteins, sugars, etc.)  As the solvent inside all cells, in blood, and in plant sap, water dissolves an enormous variety of solutes necessary for life. f) Nonpolar gasses are poorly soluble ...
Lecture 1 - Trinity College Dublin
Lecture 1 - Trinity College Dublin

... General Function: to obtain O2 for use by body’s cells & to eliminate CO2 that body cells produce Encompasses two separate but related processes   Internal ...
The basement membrane anchors epithelium to the
The basement membrane anchors epithelium to the

... The primary function of the basement membrane is to anchor down the epithelium to its loose connective tissue underneath. This is achieved by cell-matrix adhesions through substrate adhesion molecules (SAMs). The basement membrane also acts as a mechanical barrier, preventing malignant cells from in ...
Respiration, Circulation and Excretion
Respiration, Circulation and Excretion

... throughout the body, since all the organs need blood. Ask students to read the text and answer Question 1 out loud. The Circulatory System Ask students to look at the Diagram of Blood Circulation. Ask What happens to the blood when it passes through the blood vessels of the lungs? (It exchanges carb ...
The Cardiovascular System: The Blood
The Cardiovascular System: The Blood

... endothelial surfaces of a blood vessel resulting from atherosclerosis, which is induce adhesion of platelets. Intravascular clots may form when blood flows too slowly (stasis). Clotting in an unbroken blood vessel is called thrombosis. Thrombolytic agents are chemical substances that are injected in ...
The connection of cytoskeletal network with plasma membrane and
The connection of cytoskeletal network with plasma membrane and

... spatially and temporarily regulating the transportation and deposition of cell wall components. This tight control is achieved by the dynamic behavior of the cytoskeletons, but also through the tethering of these structures to the plasma membrane. This tethering may also extend beyond the plasma mem ...
Stochastic gene expression as a molecular switch for viral latency
Stochastic gene expression as a molecular switch for viral latency

... that can elude the action of antibiotics [17]. While the persister strategy lowers the average colony growth rate under normal conditions, it pays off if E. coli randomly encounters antibiotics in its environment since the persister cells survive with high probability and resume normal growth after ...
Full text in pdf format
Full text in pdf format

... Our observation that some size fractions of bacterial biomass available for protists can vary considerably (Fig. 2) indicates that the mean cell size of a bacterial assemblage may be a rather misleading parameter and does not correspond to the size classes of bacterioplankton which dominate biomass. ...
Role of E-cadherin and other cell adhesion molecules in survival
Role of E-cadherin and other cell adhesion molecules in survival

... specify the pluripotent state of both mouse and human pluripotent stem cells.9 Other regulatory cues, such as supporting cells,1,10,11 extracellular matrix,12,13 low oxygen culture,14,15 growth factors,16-18 small molecules19-23 and various signaling pathways24-29 have also been extensively studied ...
Regeneration and the need for simpler model organisms
Regeneration and the need for simpler model organisms

... of cells lost to normal ‘wear and tear’ (cell turnover), and/or injury. This attribute is of particular significance to organisms possessing relatively long lifespans, as maintenance of all body parts and their functional integration is essential for their survival. Because tissue replacement is bro ...
Loss of mitochondrial membrane potential triggers the
Loss of mitochondrial membrane potential triggers the

... of aging. Aging is a complicated multifactorial process and a number of major determinants have been described. These include genome stability, metabolism, stress resistance, and chromatindependent gene regulation, in addition to random (stochastic) events (Jazwinski, 1999, 2005). We have previously ...
Responses Mediated Downregulation of B Cell
Responses Mediated Downregulation of B Cell

... markers of the low-grade inflammation observed with aging. Agerelated alterations in responses to immune stimulation (e.g., chronic T cell stimulation with viruses, such as CMV) also contribute to low-grade inflammation by increasing the level of proinflammatory mediators, such as TNF-a (10). Produc ...
Unit 49 - Advanced Skills for AEMT
Unit 49 - Advanced Skills for AEMT

... – Changes in perfusion – Changes in metabolic activity ...
Analysis of chlorophyll fluorescence reveals stage specific patterns
Analysis of chlorophyll fluorescence reveals stage specific patterns

... INTRODUCTION ...
A protein kinase target of a PDK1 signalling pathway is involved in
A protein kinase target of a PDK1 signalling pathway is involved in

... The hydrophobic PIF domain of AGC2-1 is required both for AtPDK1 binding and for full activity Next, we investigated whether interaction of AGC2-1 with AtPDK1 requires its hydrophobic PIF domain in vivo and how this interaction affects the enzyme activity of AGC2-1. For this, we cotransfected a Myc- ...
BIM1 Encodes a Microtubule-binding Protein in Yeast.
BIM1 Encodes a Microtubule-binding Protein in Yeast.

... also was found to have more subtle defects in spindle morphology (Trueheart et al., 1987). However, bik1 mutants display synthetic lethality with tubulin mutations (Berlin et al., 1990) as well as mutations in other genes. A particularly interesting genetic characteristic of BIK1 is that overexpress ...
Friday October 10, 2014 (SW41 2014)
Friday October 10, 2014 (SW41 2014)

... EASD president Andrew Boulton, MD, of the University of Manchester, United Kingdom, who chaired the press conference, said: "This is an observational study, it isn't a randomized controlled trial, so it's difficult to draw true conclusions. But I think that pump-delivered insulin is the best form of ...
N-Linked glycans on dengue viruses grown in mammalian and
N-Linked glycans on dengue viruses grown in mammalian and

... studies have examined the phenotype of E protein glycanmutant DENVs and shown that the glycan at N153 is not necessary for virus production and spread in either mosquito or mammalian cells (Bryant et al., 2007; Miller et al., 2008). N67 was essential for virus spread in mammalian cells, which is con ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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