Association of Calmodulin and an Unconventional Myosin with the
... 1:10 in TBS, for 2 h at room temperature. After 3 x 5 min washes in TBS and a final 5-rain wash in 0.1 M sodium phosphate buffer (pH 7.2), the cover slip was fixed with 2.5% glutaraldehyde in the same phosphate buffer (45 rain, room temperature), then washed 3 x 10 rain in the same buffer. Postfixat ...
... 1:10 in TBS, for 2 h at room temperature. After 3 x 5 min washes in TBS and a final 5-rain wash in 0.1 M sodium phosphate buffer (pH 7.2), the cover slip was fixed with 2.5% glutaraldehyde in the same phosphate buffer (45 rain, room temperature), then washed 3 x 10 rain in the same buffer. Postfixat ...
Document
... b) Extravascular haemolysis Extravascular haemolysis occurs when RBC are phagocytosed by macrophages in the spleen, liver and bone marrow. When RBC are degraded within the macrophages, no free haemoglobin is released into the circulation. As a result, there is no haemoglobinaemia or haemoglobinuria ...
... b) Extravascular haemolysis Extravascular haemolysis occurs when RBC are phagocytosed by macrophages in the spleen, liver and bone marrow. When RBC are degraded within the macrophages, no free haemoglobin is released into the circulation. As a result, there is no haemoglobinaemia or haemoglobinuria ...
Taxonomy, biology and physiology of fungi
... B. reproduce by means of spores (conidia), usually wind-disseminated C. both sexual (meiotic) and asexual (mitotic) spores may be produced, depending on the species and conditions D. typically not motile, although a few (e.g. Chytrids) have a motile phase. E. like plants, may have a stable haploid & ...
... B. reproduce by means of spores (conidia), usually wind-disseminated C. both sexual (meiotic) and asexual (mitotic) spores may be produced, depending on the species and conditions D. typically not motile, although a few (e.g. Chytrids) have a motile phase. E. like plants, may have a stable haploid & ...
IFN and IL12 synergize to convert in vivo generated Th17 into Th1
... control of inflammatory immune responses. Th cells coexpressing IFN-c and IL-17 have been observed in vivo, but it remained elusive, how these cells had been generated and whether they represent a distinct lineage of Th differentiation. It has been shown that ex vivo isolated Th1 and Th17 cells are ...
... control of inflammatory immune responses. Th cells coexpressing IFN-c and IL-17 have been observed in vivo, but it remained elusive, how these cells had been generated and whether they represent a distinct lineage of Th differentiation. It has been shown that ex vivo isolated Th1 and Th17 cells are ...
CH32IntroCharacteristicsPart2
... • An animal's skeleton provides a framework that supports the animal's body and is vital to an animal's movement. • Many soft-bodied invertebrates, such as jellyfish, have a hydrostatic skeleton, which is a water-filled cavity that is under pressure. (Balloon) • An exoskeleton is a rigid external sk ...
... • An animal's skeleton provides a framework that supports the animal's body and is vital to an animal's movement. • Many soft-bodied invertebrates, such as jellyfish, have a hydrostatic skeleton, which is a water-filled cavity that is under pressure. (Balloon) • An exoskeleton is a rigid external sk ...
Cell cycle control of septin ring dynamics in the
... the budding cell into a morphogenetically active daughter side and a morphogenetically inactive mother side (Barral et al., 2000). However, the relationship between septin filaments assembly and maintenance and cell cycle regulatory mechanisms is still poorly understood. In this work we study the ce ...
... the budding cell into a morphogenetically active daughter side and a morphogenetically inactive mother side (Barral et al., 2000). However, the relationship between septin filaments assembly and maintenance and cell cycle regulatory mechanisms is still poorly understood. In this work we study the ce ...
When cells get stressed: an integrative view of cellular senescence
... stress, “oncogenic stress” (due to oncogene overexpression), and other metabolic perturbations (5, 18–22). Typically, these forms of senescence do not involve significant telomere shortening and cannot be prevented by ectopic hTERT expression (23, 24). Accordingly, the hypothesized telomere-based me ...
... stress, “oncogenic stress” (due to oncogene overexpression), and other metabolic perturbations (5, 18–22). Typically, these forms of senescence do not involve significant telomere shortening and cannot be prevented by ectopic hTERT expression (23, 24). Accordingly, the hypothesized telomere-based me ...
When cells get stressed: an integrative view of cellular
... stress, “oncogenic stress” (due to oncogene overexpression), and other metabolic perturbations (5, 18–22). Typically, these forms of senescence do not involve significant telomere shortening and cannot be prevented by ectopic hTERT expression (23, 24). Accordingly, the hypothesized telomere-based me ...
... stress, “oncogenic stress” (due to oncogene overexpression), and other metabolic perturbations (5, 18–22). Typically, these forms of senescence do not involve significant telomere shortening and cannot be prevented by ectopic hTERT expression (23, 24). Accordingly, the hypothesized telomere-based me ...
DRP1 family in cytokinesis and cell expansion
... Although sucrose-rescued adl1A mutants display normal vegetative growth, they are highly infertile as a result of a maternal sporophytic defect in the mutant flowers that severely inhibits fertilization (Kang et al., 2001). We reasoned that the maternal defect could result from problems in the mutan ...
... Although sucrose-rescued adl1A mutants display normal vegetative growth, they are highly infertile as a result of a maternal sporophytic defect in the mutant flowers that severely inhibits fertilization (Kang et al., 2001). We reasoned that the maternal defect could result from problems in the mutan ...
intraosseous infusion, ez-io device
... Medical Director as an EMT-P. c. “Intraosseous Infusion Drill Device” i.e. EZ-IO, means a small batterypowered device and two beveled, hollow drill-tipped needles that were specifically designed to provide safe, controlled vascular access via the intraosseous route to patients of all ages in emergen ...
... Medical Director as an EMT-P. c. “Intraosseous Infusion Drill Device” i.e. EZ-IO, means a small batterypowered device and two beveled, hollow drill-tipped needles that were specifically designed to provide safe, controlled vascular access via the intraosseous route to patients of all ages in emergen ...
Plasmodesmata-Mediated Cell-to-Cell Communication in the Shoot
... there. It is widely accepted that macromolecular signals such as TFs and small non-coding RNA (sRNAs) act as mobile signals that are actively and selectively transported to target cells through PD to specify their fates [18]. Around twenty years ago, the first mobile signal moving between cells via ...
... there. It is widely accepted that macromolecular signals such as TFs and small non-coding RNA (sRNAs) act as mobile signals that are actively and selectively transported to target cells through PD to specify their fates [18]. Around twenty years ago, the first mobile signal moving between cells via ...
High-throughput knockout screen in Schizosaccharomyces pombe
... fundamentally different and ensures segregation of homologous centromeres. Paliulis and Nicklas demonstrated that chromosome segregation during meiosis is determined by special properties of the meiotic chromosomes rather than by spindle components or other cytosolic factors.1 There are three major ...
... fundamentally different and ensures segregation of homologous centromeres. Paliulis and Nicklas demonstrated that chromosome segregation during meiosis is determined by special properties of the meiotic chromosomes rather than by spindle components or other cytosolic factors.1 There are three major ...
Muramatsu M
... rounds of glutathione-agarose chromatography. The purified GST fusion protein was confirmed by Western blot analyses as described previously (26) using anti-AID antisera, which were prepared by immunizing rabbits with a multiple-antigen peptide (27) containing residues 185–198 (EVDDLRDAFRMLGF). Cyti ...
... rounds of glutathione-agarose chromatography. The purified GST fusion protein was confirmed by Western blot analyses as described previously (26) using anti-AID antisera, which were prepared by immunizing rabbits with a multiple-antigen peptide (27) containing residues 185–198 (EVDDLRDAFRMLGF). Cyti ...
Developmental genetics of the Caenorhabditis elegans pharynx
... five types of pharyngeal cells: neurons (20), muscles (20 cells; 37 nuclei), marginal cells (9), epithelial cells (9), and gland cells (4 cells; 5 nuclei). The muscle cells and marginal cells form a single-cell-thick tube with trifold symmetry (two bilateral symmetrical left and right subventral sid ...
... five types of pharyngeal cells: neurons (20), muscles (20 cells; 37 nuclei), marginal cells (9), epithelial cells (9), and gland cells (4 cells; 5 nuclei). The muscle cells and marginal cells form a single-cell-thick tube with trifold symmetry (two bilateral symmetrical left and right subventral sid ...
Chemotherapy Targets the Hair-Follicle Vascular
... Before depilation (telogen), the ND-GFP-expressing cells were located in the follicular bulge area and blood vessel network. The blood vessel network interconnected hair follicles via their bulge area (Figure 1b). At day 6 postdepilation (p.d.) (middle anagen), ND-GFP-expressing outerroot sheath cel ...
... Before depilation (telogen), the ND-GFP-expressing cells were located in the follicular bulge area and blood vessel network. The blood vessel network interconnected hair follicles via their bulge area (Figure 1b). At day 6 postdepilation (p.d.) (middle anagen), ND-GFP-expressing outerroot sheath cel ...
Distinct Actions and Cooperative Roles of ROCK
... ROCK-D1-induced formation of stress fibers was weaker than the V14RhoA-induced one, and the ROCK-D1-induced stress fibers were morphologically different from the V14RhoA-induced ones. The ROCK-D1-induced stress fibers showed stellate-like morphology (stellate stress fibers) at the basal levels, and ...
... ROCK-D1-induced formation of stress fibers was weaker than the V14RhoA-induced one, and the ROCK-D1-induced stress fibers were morphologically different from the V14RhoA-induced ones. The ROCK-D1-induced stress fibers showed stellate-like morphology (stellate stress fibers) at the basal levels, and ...
Fatty acids as gatekeepers of immune cell regulation - Direct-MS
... forms of lipid trafficking exist, which are as yet poorly understood. This Review examines the nature and regulation of cellular lipid pools in the immune system, their delivery of fatty acids or fatty acid derivatives to specific locations and their potential role in health and disease. Fatty acids ...
... forms of lipid trafficking exist, which are as yet poorly understood. This Review examines the nature and regulation of cellular lipid pools in the immune system, their delivery of fatty acids or fatty acid derivatives to specific locations and their potential role in health and disease. Fatty acids ...
The lipid phosphatase LPP3 regulates extra
... mutation miles apart results in a failure of the heart primordia to migrate to the midline and subsequently fuse. The altered gene encodes a protein with high homology to the ...
... mutation miles apart results in a failure of the heart primordia to migrate to the midline and subsequently fuse. The altered gene encodes a protein with high homology to the ...
Lymphoid Organs in Mice T Cells from Gut
... the migratory fate of CD8+ T cells that do not meet their cognate Ag remains elusive. These naive lymphocytes, which make up most CD8+ T lymphocytes in secondary lymphoid organs, are exposed to proinflammatory cytokines secreted by activated dendritic cells (DCs) in the inflamed lymphoid tissue mili ...
... the migratory fate of CD8+ T cells that do not meet their cognate Ag remains elusive. These naive lymphocytes, which make up most CD8+ T lymphocytes in secondary lymphoid organs, are exposed to proinflammatory cytokines secreted by activated dendritic cells (DCs) in the inflamed lymphoid tissue mili ...
9–1 Chemical Pathways - Springboro Community Schools
... As you can see, cellular respiration requires oxygen, a food molecule such as glucose, and gives off carbon dioxide, water, and energy. Do not be misled, however, by the simplicity of this equation. If cellular respiration took place in just one step, all of the energy from glucose would be released ...
... As you can see, cellular respiration requires oxygen, a food molecule such as glucose, and gives off carbon dioxide, water, and energy. Do not be misled, however, by the simplicity of this equation. If cellular respiration took place in just one step, all of the energy from glucose would be released ...
Ch21_lecture
... • The small intestine is the major site of nutrient absorption into the blood. • It has numerous folds and projections that give it an internal surface area 600 times greater than a smooth tube of the same length. • Fingerlike projections called villi (singular, villus) cover the entire surface of t ...
... • The small intestine is the major site of nutrient absorption into the blood. • It has numerous folds and projections that give it an internal surface area 600 times greater than a smooth tube of the same length. • Fingerlike projections called villi (singular, villus) cover the entire surface of t ...
The Effect of Osmotic Shock on Release of Bacterial Proteins and on
... called the shock fluid, is removed. This fluid contains the hydrolytic enzymes already referred to (Table I) as well as the binding proteins. It contains about 3.5 % of the cellular protein when the cells are grown under conditions that suppress the synthesis of alkaline phosphatase. T h e shocked c ...
... called the shock fluid, is removed. This fluid contains the hydrolytic enzymes already referred to (Table I) as well as the binding proteins. It contains about 3.5 % of the cellular protein when the cells are grown under conditions that suppress the synthesis of alkaline phosphatase. T h e shocked c ...
Detailed Lecture Outline
... In detail, arterial blood pressure in the systemic circuit is not constant. With each heartbeat, pressure rises to a peak during ventricular systole (systolic pressure) and a minimum during diastole (diastolic pressure). The difference between the systolic pressure and the diastolic pressure is th ...
... In detail, arterial blood pressure in the systemic circuit is not constant. With each heartbeat, pressure rises to a peak during ventricular systole (systolic pressure) and a minimum during diastole (diastolic pressure). The difference between the systolic pressure and the diastolic pressure is th ...
Glypican-1 Is a Vehicle for Polyamine Uptake in Mammalian Cells
... (Ambion Inc., Austin, TX) containing the sequence GCTGGTCTACTGTGCTCAC (corresponding to nucleotides 977–995 in human Gpc-1) followed by the hairpin sequence TTCAAGAGA and then the reversed complementary Gpc-1 sequence with an additional C in the 5⬘-end and a stretch of six Ts for RNA polymerase III ...
... (Ambion Inc., Austin, TX) containing the sequence GCTGGTCTACTGTGCTCAC (corresponding to nucleotides 977–995 in human Gpc-1) followed by the hairpin sequence TTCAAGAGA and then the reversed complementary Gpc-1 sequence with an additional C in the 5⬘-end and a stretch of six Ts for RNA polymerase III ...