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The Effect of Osmotic Shock on Release of Bacterial Proteins and on
The Effect of Osmotic Shock on Release of Bacterial Proteins and on

... called the shock fluid, is removed. This fluid contains the hydrolytic enzymes already referred to (Table I) as well as the binding proteins. It contains about 3.5 % of the cellular protein when the cells are grown under conditions that suppress the synthesis of alkaline phosphatase. T h e shocked c ...
FUNCTIONAL DESIGN OF THE RESPIRATORY SYSTEM
FUNCTIONAL DESIGN OF THE RESPIRATORY SYSTEM

... capillaries cannot be as well arterialized as with the flow-through system of the avian model. In addition, having a common pathway for inspiration and expiration means that part of the inhaled air will not participate to gas exchange*, [*The larger the volume of the conductive airways (also called ...
Spermine Acts as a Negative Regulator of
Spermine Acts as a Negative Regulator of

... phenotype (3– 6). This latter capability raised the notion that PMA phagocytosis was assessed by the ability of the cells to ingest fluorescent beads at 21 hours after treatment initiation, as described previously (20). For testing may function as a differentiation therapy drug. Because of this post ...
Cell-surface location of Listeria-specific protein p60
Cell-surface location of Listeria-specific protein p60

... of immunogold-labelled cells (Fig. 3 d ) . The maximum density of gold grains on the cell surface was estimated to be about 300 per pm2 for both ATCC 19111 and the rough mutant SLCC 5782. The specificity of immunogold labelling was established by control experiments in which the anti-p60 antiserum w ...
Fibronectin and radial intercalation
Fibronectin and radial intercalation

... Keller and Jansa have further shown that involution and dorsal mesoderm extension occur even in the absence of a BCR (Keller and Jansa, 1992), suggesting that the primary mechanism regulating dorsal mesoderm movement in Xenopus is convergent extension. However, others have reported that inhibition o ...
Mitochondria use actin filaments as rails for fast translocation in
Mitochondria use actin filaments as rails for fast translocation in

... mitochondria moving slowly along microtubules (0.220.05 m m s1), while in our study, mitochondria movement along microtubules was less than our limit of detection (0.3 m m s1). In any case, our in vivo study shows that mitochondria and peroxisomes in Arabidopsis and tobacco move quickly and over ...
An Equatorial Contractile Mechanism Drives Cell Elongation but not
An Equatorial Contractile Mechanism Drives Cell Elongation but not

... the establishment of an equatorial actomyosin ring is preceded and regulated by many cell cycle events, and the ring itself is a complex and dynamic structure. Here we report the presence of an equatorial circumferential actomyosin structure with remarkable similarities to the cytokinetic ring forme ...
Steven Lindow - Biocontrol 2016
Steven Lindow - Biocontrol 2016

... Disease control The goal: Confuse pathogen by exposing it to excessive amounts of signal molecule even when it is in low population sizes The expectation: The premature presence of DSF in vessels ...
A Genome-Wide Screen for Bacterial Envelope Biogenesis Mutants
A Genome-Wide Screen for Bacterial Envelope Biogenesis Mutants

... another so that the envelope grows uniformly and maintains its integrity as it is remodeled. Given that genes coding for envelope proteins constitute roughly one quarter of the E. coli genome, and that over a third of these have an unknown or poorly understood function [12], it is likely that many f ...
Factors Influencing the Production of Water
Factors Influencing the Production of Water

The UNC-112 gene in Caenorhabditis elegans Encodes a Novel
The UNC-112 gene in Caenorhabditis elegans Encodes a Novel

... B.Sc,, Ph.D., Simon Fraser Postdoc, Washington University School of Medicine Visiting Scientist, Max-Planck Institute for Biochemistry ...
Swimming behavior of the monotrichous bacterium Pseudomonas
Swimming behavior of the monotrichous bacterium Pseudomonas

... output. The cells of P. fluorescens SBW25 are monotrichous as expected, but not in sensu stricto. More than 60% flagellated cells are with one flagellum (Fig. 1a inset). Among the 254 fluorescence-labeled cells examined, c. 38% carry more than two flagella. The average number of flagella per cell is ...
Ethanol Induces Heterotopias in Organotypic
Ethanol Induces Heterotopias in Organotypic

... appeared normal, i.e. as in the untreated slices. In instances where the pial or ventricular surface was broached, cells were found beyond the normal boundaries in the formation of warts (Fig. 2). Two types of warts were identified: peduncular and appositional. In peduncular warts, the cells formed ...
Novel Symbiotic Protoplasts Formed by Endophytic Fungi Explain
Novel Symbiotic Protoplasts Formed by Endophytic Fungi Explain

... Diverse fungi live all or part of their life cycle inside plants as asymptomatic endophytes. While endophytic fungi are increasingly recognized as significant components of plant fitness, it is unclear how they interact with plant cells; why they occur throughout the fungal kingdom; and why they are ...
Visualization of the moonlighting protein CD26DPPIV - UvA-DARE
Visualization of the moonlighting protein CD26DPPIV - UvA-DARE

... 1; for review see Van Noorden and Butcher 1991), because they were not able to use tools of modern cytochemistry such as synthetic substrates. However, as stated above, NAD(P)H is produced by a series of enzymes and is used by many others involved in various metabolic processes such as the respirato ...
Co-ordination of signalling elements in guard cell ion
Co-ordination of signalling elements in guard cell ion

... been estimated K+/Cl− selectivity of this channel in barley mesophyll to be as high as 3051, indicating the importance of this channel for tonoplast electrogenesis and K+ transport ( Tikhonova et al., 1997). It now appears that all three cation channels co-exist in the guard cell vacuole (Allen and ...
Fig. - Journal of Cell Science
Fig. - Journal of Cell Science

... levels of transfection efficiency. Importantly, this approach introduces constitutively expressed Gli1 together with the Gli1dependent reporter construct into the cells. This allows us to pinpoint the stage at which USP21 could regulate Hh signaling, downstream of SMO activation – i.e. downstream of ...
15. THYROID FUNCTION Follicular activity
15. THYROID FUNCTION Follicular activity

... inactive by removing iodine from their structure with the help of deiodinases (Fig. 15-17). The best example is the conversion of T4 into rT3, if the cell does not require stimulation. To get rid of the thyronine, the liver conjugates the molecule with a glucoronide or sulphate molecule making it mo ...
15. THYROID FUNCTION Follicular activity
15. THYROID FUNCTION Follicular activity

... function by increasing heart rate and the force of contraction, which in turn translates to an elevation in blood pressure and an increase in cardiac output (Fig. 1516). ...
ER and vacuoles: never been closer
ER and vacuoles: never been closer

... 2000; Robinson and Rogers, 2000). These vesicles would homotypically fuse to form tubular structures that represent the precursors of vacuoles, the provacuoles. The tubular provacuoles are supposed to fuse with one another, forming a complex network that finally will give raise to the central vacuole ...
42 BIOLOGY 1. Circulation in the Animal Kingdom
42 BIOLOGY 1. Circulation in the Animal Kingdom

... Circulatory systems link exchange surfaces with cells throughout the body  Small molecules can move between cells and their surroundings by diffusion  Diffusion is only efficient over small distances because the time it takes to diffuse is proportional to the square of the distance  In small or t ...
Mob4 plays a role in spindle focusing in Drosophila S2 cells
Mob4 plays a role in spindle focusing in Drosophila S2 cells

... As reported previously (Goshima and Vale, 2003), S2 cells often show aberrant mitotic spindle phenotypes. Some S2 cells form monopolar spindles that subsequently can be converted to a bipolar spindle by a rescue process in which microtubules become organized to form a second, acentrosomal pole (~10% ...
Delamination of neural crest cells requires transient
Delamination of neural crest cells requires transient

... long been recognised as crucial for the generation of tissues and organs in both vertebrates and invertebrates. However, because EMT converts epithelial cells into migratory and invasive mesenchymal cells, it has also been established as an important step in the metastatic cascade of tumours (Nieto, ...
18. THYROID FUNCTION
18. THYROID FUNCTION

... mode continues until the follicle becomes distended and filled with colloid. Once a follicle is filled the cells surrounding that follicle produce and exteriorize receptors for Thyroid Stimulation Hormone, (TSH) a glycoprotein hormone consisting of two subunits (Fig. 18-5). When TSH attaches to thes ...
2015 – PKc-theta is a novel sc35 splicing factor regulator in
2015 – PKc-theta is a novel sc35 splicing factor regulator in

... T cell genes, such as CD44 and CD45, undergo alternative splicing to produce distinct protein isoforms (7, 8). Furthermore, antigenic stimulation alters the pattern of alternative splicing to produce significant functional changes in protein expression (8, 9). Recent global studies of alternative sp ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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