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... 50 ng/ml of the co-injection marker pL15EK (Moresco and Koelle 2004) into MT8189 animals. For overexpression of C. elegans TRPV genes, a genomic region for each was amplified (GeneAmp XL, Applied Biosystems, Foster City, CA), containing coding regions along with 59 and 39 regulatory regions extendin ...
stem cells and society - Worcester Polytechnic Institute
stem cells and society - Worcester Polytechnic Institute

... As their name implies, adult stem cells (ASCs) are isolated from adult tissues. These cells exist within various tissue of the developed human body, and are also called somatic stem cells. Adult stem cells have been found in the “brain, bone marrow, peripheral blood, blood vessels, skeletal muscle, ...
(2002) Thyroxine induces pancreatic beta cell apoptosis in rats
(2002) Thyroxine induces pancreatic beta cell apoptosis in rats

... the hyperthyroid state puts an increased demand on each single remaining beta cell, which have to compensate for the increased insulin requirements due to thyroxine-induced peripheral insulin resistance [2]. An increase of the blood glucose concentration which typically accompanies the hyperthyroid ...
Development
Development

... Plants have diversified their leaf morphologies to adapt to diverse ecological niches. The molecular components responsible for regulating leaf morphology, however, have not been fully elucidated. By screening Arabidopsis activation-tagging lines, we identified a dominant mutant, which we designated l ...
Neonatal Acute Kidney Injury - Lucknow Academy of Pediatrics
Neonatal Acute Kidney Injury - Lucknow Academy of Pediatrics

... Dose needs to be modified as per creatinine clearance 1st dose / loading dose needn’t be modified, subsequent doses should be ...
Introduction to Conventional Chemotherapy
Introduction to Conventional Chemotherapy

... Cisplatin Toxicity • Nephrotoxicity – dose-limiting toxicity – renal damage is usually reversible but rarely can be irreversible and require dialysis – platinum concentrations are higher in the kidney than in the plasma or other tissues – initiating event is proximal tubular lesion – secondary even ...
Diffusion of Green Fluorescent Protein in Three Cell
Diffusion of Green Fluorescent Protein in Three Cell

... clipping at the C terminus, but there is no indication that the intact GFP domain can be excised. Thus, we can be confident that all the GFP fluorescence seen in the transformant originates from the TatA-GFP fusion (19). We used qualitative FRAP to show that dispersed material diffuses freely, where ...
pharmacognostical evaluation of katuka ( picrorhiza kurroa royle ex
pharmacognostical evaluation of katuka ( picrorhiza kurroa royle ex

... In present time, to ensure the safety and efficacy of Ayurvedic medicines, there is a great need of quality control. Foremost component of quality control is the accurate identification of the raw drug. Because, if the quality of raw drug is bad, the product is bound to be bad. Now a days, one of th ...
Jellies - ancient ocean drifters
Jellies - ancient ocean drifters

... In the open oceans, some 2000 species of jellies pulsate with life as they drift with the ocean currents. The variety of designs and the luminescent colours of these otherworldly creatures will astound even the most fertile of imaginations. In fact it is said that some of the weird futuristic design ...
CHAPTER 1-2: BIOLOGICAL ORGANIZATION CHAPTER 1
CHAPTER 1-2: BIOLOGICAL ORGANIZATION CHAPTER 1

... For every molecule of acetyl CoA that enters the Krebs cycle, ——— NADH molecules (among other molecule types) are produced. four B three C two D five ...
Physiological effects of shortwave diathermy
Physiological effects of shortwave diathermy

... treatments of shortwave diathermy and joint mobilizations have demonstrated increased range of motion over 10 degrees in the shoulder. Wound healing Shortwave diathermy increases white blood cell infiltration and increases phagocytosis (phagocytosis is where neutrofils and macrophages engulf cellula ...
Hepatosplenic gamma/delta T-Cell Lymphoma in Bone Marrow A
Hepatosplenic gamma/delta T-Cell Lymphoma in Bone Marrow A

... histiocytes was seen in 4 cases (cases 2, 3, 4, and 8). With disease progression, the degree of interstitial infiltration ❚Image 3A❚ and ❚Image 3B❚ and the percentage of medium to large cells increased ❚Image 3C❚, although a subpopulation of small atypical cells with irregular nuclear contours and c ...
Flow Cell Design for Effective Biosensing
Flow Cell Design for Effective Biosensing

... explosives [4]. A common approach is to use flow cells with biosensors constructed upon screen printed electrodes, providing a compact analysis system applicable for both laboratory based analysis and on-site use [5–8]. Flow cells can either be purpose-built for the biosensor of interest [9] or bios ...
Assembly of Trunk and Limb Blood Vessels Involves Extensive
Assembly of Trunk and Limb Blood Vessels Involves Extensive

... It seemed feasible to address questions of angioblast migration and assembly using mammalian tissue as a source of precursor cells, since mouse cells can be genetically marked and mouse vascular cells express the marker PECAM-1 (Baldwin et al., 1994). Mouse somite transplantation experiments suggest ...
MECHANISMS OF PATTERN FORMATION IN PLANT
MECHANISMS OF PATTERN FORMATION IN PLANT

... the ommatidia in the eye of Drosophila and the specification of neuroblasts by DELTA-NOTCH interactions) occurs by a process called lateral inhibition, in which a noncell-autonomous signal from a differentiating cell influences the differentiation choice of immediately adjacent cells (11). 4. Gradie ...
The Spatial Pattern of Cochlear Amplification
The Spatial Pattern of Cochlear Amplification

... about by an interplay between longitudinal fluid coupling and local displacement of the cochlear partition, whose stiffness and mass are graded (Lighthill, 1981). In a normal ear, an active process in outer hair cells amplifies and sharpens the traveling wave, thereby fostering the remarkable freque ...
GCSE Biology Specification (For teaching from 2016
GCSE Biology Specification (For teaching from 2016

... humans and the mechanisms by which larger molecules are broken down into smaller soluble molecules which can be absorbed into the blood. There is also consideration of a balanced diet and the effects of excess sugar and fat in the diet. Working Scientifically The use of Visking tubing as a model of ...
Transport of virally expressed green fluorescent protein through the
Transport of virally expressed green fluorescent protein through the

... the ER of tobacco leaf cells using the patatin signal peptide (Boevink et al. 1996). Here we report on the increased eciency of the sporamin signal peptide for insertion of GFP-KDEL into the ER and on the secretion of GFP, without KDEL, to the apoplast. In addition, we show that both the inhibitor ...
Association of Calmodulin and an Unconventional Myosin with the
Association of Calmodulin and an Unconventional Myosin with the

... 1:10 in TBS, for 2 h at room temperature. After 3 x 5 min washes in TBS and a final 5-rain wash in 0.1 M sodium phosphate buffer (pH 7.2), the cover slip was fixed with 2.5% glutaraldehyde in the same phosphate buffer (45 rain, room temperature), then washed 3 x 10 rain in the same buffer. Postfixat ...
Long-Term Persistent Vesicular Stomatitis Virus and Rabies Virus
Long-Term Persistent Vesicular Stomatitis Virus and Rabies Virus

... sizes are often present in addition to, or in place of, the very long T particles. Fig. I shows an example of some of the patterns of T particles seen after co-cultivation of the B H K 2i, VSV~nd CARd carrier with uninfected B H K zI cells during the period from I½ to 2½ years after establishment of ...
Dynamic Localization of Rop GTPases to the
Dynamic Localization of Rop GTPases to the

... Department of Botany and Plant Sciences, University of California, Riverside, California 92521 (Y.L., Z.Y.); and Department of Biology, Indiana University-Purdue University, Indianapolis, Indiana 46202 (D.F.S., S.K.R.) Vacuoles are essential pleomorphic organelles that undergo dynamic changes during ...
Changes  in  some factors of the  innate... and  serum  zinc and  iron  concentrations ...
Changes in some factors of the innate... and serum zinc and iron concentrations ...

... and lysozyme with the object of controlling the infection until an immune response can be mobilized. Phagocytosis by white blood cells (monocytes and neutrophils) and tissue macrophages is one of the dominant defence mechanisms and there is no doubt that LPS suppresses it (Wonderling, Ghaffar & Maye ...
KIR2DS1-Positive NK Cells Mediate Alloresponse against the C2
KIR2DS1-Positive NK Cells Mediate Alloresponse against the C2

... four inhibitory KIRs that have ligand specificity for codon sequences present in only some HLA class I alleles: KIR2DL1, 2DL2, 2DL3, and 3DL1. The 2DL2 and 2DL3 receptors, whose respective genes share an allelic relationship, recognize with rare exceptions the HLA-Cw molecules with Ser77Asn80 in the ...
4T, 3T AND 3T1D DRAM CELL DESIGN ON 32 NM
4T, 3T AND 3T1D DRAM CELL DESIGN ON 32 NM

... reduction in power consumption as compared to previous DRAM cell design. In order to write the cell at the BL write line level, it is only required to activate T1 through the WL write line. Hence, the S node stores either a 0 or a VDD−Vth voltage depending on the logic value. This voltage results in ...
Document
Document

... b) Extravascular haemolysis Extravascular haemolysis occurs when RBC are phagocytosed by macrophages in the spleen, liver and bone marrow. When RBC are degraded within the macrophages, no free haemoglobin is released into the circulation. As a result, there is no haemoglobinaemia or haemoglobinuria ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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