Evaluation of cell membrane integrity as a potential antimicrobial
... Bacterial cell membranes can be used as a target for the development of new antibacterial drugs. Many plants produce secondary metabolites which contain a steroid or triterpernoid aglycon attached to one or more sugar chains that exhibit cell membrane permeabilizing properties [11]. Permeability enh ...
... Bacterial cell membranes can be used as a target for the development of new antibacterial drugs. Many plants produce secondary metabolites which contain a steroid or triterpernoid aglycon attached to one or more sugar chains that exhibit cell membrane permeabilizing properties [11]. Permeability enh ...
Mapping the Synthetic Dosage Lethality Network of CDK1/CDC28
... Mapping the SDL network and comparison with previous genetic screens We systematically transformed a WT strain and a cdc28-as1 mutant strain (Bishop et al. 2000; Zimmermann et al. 2011) with the yeast movable ORF library, which is an arrayed plasmid library consisting of ~4,900 ORFs under control of ...
... Mapping the SDL network and comparison with previous genetic screens We systematically transformed a WT strain and a cdc28-as1 mutant strain (Bishop et al. 2000; Zimmermann et al. 2011) with the yeast movable ORF library, which is an arrayed plasmid library consisting of ~4,900 ORFs under control of ...
Chromatin folding – from biology to polymer models - UvA-DARE
... experimentally and their outcome can be used to further improve the model, thereby increasing our understanding of chromatin folding. In this Commentary, we demonstrate that efforts to unravel the complex and dynamic relationship between eukaryotic gene regulation and chromatin folding benefit from ...
... experimentally and their outcome can be used to further improve the model, thereby increasing our understanding of chromatin folding. In this Commentary, we demonstrate that efforts to unravel the complex and dynamic relationship between eukaryotic gene regulation and chromatin folding benefit from ...
The Kidneys and How They Work
... tubes called ureters. The bladder stores urine until releasing it through urination. Wastes in the blood come from the normal breakdown of active tissues, such as muscles, and from food. The body uses food for energy and self-repairs. After the body has taken what it needs from food, wastes are sent ...
... tubes called ureters. The bladder stores urine until releasing it through urination. Wastes in the blood come from the normal breakdown of active tissues, such as muscles, and from food. The body uses food for energy and self-repairs. After the body has taken what it needs from food, wastes are sent ...
animal phyla - Sakshieducation.com
... a) They are exclusively marine, radially symmetrical animals b) They move by cilia, which join together to form eight meridian comb plates c) When the tentacles are present they are two in number and contain cnidoblasts d) Reproduction takes place only by sexual means ...
... a) They are exclusively marine, radially symmetrical animals b) They move by cilia, which join together to form eight meridian comb plates c) When the tentacles are present they are two in number and contain cnidoblasts d) Reproduction takes place only by sexual means ...
The cell biology of hearing - The Journal of Cell Biology
... into a bundle (Fig. 2 B) and contribute to bundle passive mechanics (Bashtanov et al., 2004). Tip links project in the axis of mechanical sensitivity of the hair bundle and are thought to gate transduction channels at stereociliary tips (Pickles et al., 1984). In support of this model, the hair bund ...
... into a bundle (Fig. 2 B) and contribute to bundle passive mechanics (Bashtanov et al., 2004). Tip links project in the axis of mechanical sensitivity of the hair bundle and are thought to gate transduction channels at stereociliary tips (Pickles et al., 1984). In support of this model, the hair bund ...
Alterations in gene expression in T1α null lung: a model of deficient
... their lungs with the first few breaths [11]. In normal late fetal and adult lungs, T1α protein is uniquely expressed in the apical membrane of type I alveolar epithelial cells, which form over 90% of the alveolar surface that is specialized for gas exchange [12-14]. In the absence of this protein th ...
... their lungs with the first few breaths [11]. In normal late fetal and adult lungs, T1α protein is uniquely expressed in the apical membrane of type I alveolar epithelial cells, which form over 90% of the alveolar surface that is specialized for gas exchange [12-14]. In the absence of this protein th ...
The Kidneys and How They Work
... tubes called ureters. The bladder stores urine until releasing it through urination. Wastes in the blood come from the normal breakdown of active tissues, such as muscles, and from food. The body uses food for energy and self-repairs. After the body has taken what it needs from food, wastes are sent ...
... tubes called ureters. The bladder stores urine until releasing it through urination. Wastes in the blood come from the normal breakdown of active tissues, such as muscles, and from food. The body uses food for energy and self-repairs. After the body has taken what it needs from food, wastes are sent ...
COOL FACt Learn with BODY WORLDS
... Well, our bodies are pretty amazing. And the more we learn about ourselves and how our bodies work, the better we can take care of ourselves and others. And, the healthier we will be—making us better on the ice rink, basketball or tennis court, jumping hurdles, or just walking down the street. “Gunt ...
... Well, our bodies are pretty amazing. And the more we learn about ourselves and how our bodies work, the better we can take care of ourselves and others. And, the healthier we will be—making us better on the ice rink, basketball or tennis court, jumping hurdles, or just walking down the street. “Gunt ...
Cell cycle behavior of human HP1 subtypes: distinct
... Intracellular localization of YFP-fused HP1 subtypes We examined the localization of YFP-fusion constructs of human HP1 subtypes in HeLa cells. HeLa cells transiently expressing YFP-fused HP1 were fixed and observed under a fluorescence microscope. In interphase nuclei, YFP-fused HP1 showed two diff ...
... Intracellular localization of YFP-fused HP1 subtypes We examined the localization of YFP-fusion constructs of human HP1 subtypes in HeLa cells. HeLa cells transiently expressing YFP-fused HP1 were fixed and observed under a fluorescence microscope. In interphase nuclei, YFP-fused HP1 showed two diff ...
The Rhopalosiphum padi virus 59 internal ribosome entry site is
... These results show that the RhPV 59 IRES functions efficiently in Sf 21 cells and in Sf 21 lysates; the RhPV 59 IRES also functions well in a Drosophila lysate (Woolaway et al., 2001). In contrast, the EMCV IRES is inactive in each of these systems (Finkelstein et al., 1999; Woolaway et al., 2001). ...
... These results show that the RhPV 59 IRES functions efficiently in Sf 21 cells and in Sf 21 lysates; the RhPV 59 IRES also functions well in a Drosophila lysate (Woolaway et al., 2001). In contrast, the EMCV IRES is inactive in each of these systems (Finkelstein et al., 1999; Woolaway et al., 2001). ...
Quantitative profiling of initiating ribosomes in vivo - Shu
... was found between the differences of LTM-captured aTIS density and the ribosome occupancy on the coding region (CDS) (Supplementary Fig. 1c). The lack of quantitative features in GTI-seq largely hampers comparative interrogation of translational regulation. To circumvent this limitation, we set out ...
... was found between the differences of LTM-captured aTIS density and the ribosome occupancy on the coding region (CDS) (Supplementary Fig. 1c). The lack of quantitative features in GTI-seq largely hampers comparative interrogation of translational regulation. To circumvent this limitation, we set out ...
Role of the RNA-binding protein Roquin in immune homeostasis
... of all T-dependent isotypes. Despite normal T and B cell development, effector-like CD4 and CD8 T cells accumulate in the periphery of adult mice. In the Rc3h1san/san mice the M199R is a hypomorphic rather than null mutation, since forced expression of Rc3h1M199R in san/san T cells induced an interm ...
... of all T-dependent isotypes. Despite normal T and B cell development, effector-like CD4 and CD8 T cells accumulate in the periphery of adult mice. In the Rc3h1san/san mice the M199R is a hypomorphic rather than null mutation, since forced expression of Rc3h1M199R in san/san T cells induced an interm ...
Cellular Dynamics of Cholinergically Inducedα(8 –13 Hz) Rhythms
... LGN TC neuron in vitro causes a rapid depolarization that is initially associated with a profound reduction in input resistance RN (2) consisting of only two to four spikes but later with an increase in RN (3). In this cell, the depolarization brought about by Cch shifts the cell to just below the t ...
... LGN TC neuron in vitro causes a rapid depolarization that is initially associated with a profound reduction in input resistance RN (2) consisting of only two to four spikes but later with an increase in RN (3). In this cell, the depolarization brought about by Cch shifts the cell to just below the t ...
Live Cell Imaging Reveals Structural Associations
... MTs using the drug jasplakinolide (Bubb et al., 1994). Because jasplakinolide has not been extensively employed in plant cell biology, we first grew Arabidopsis seedlings on different concentrations of the drug and assessed the impact on growth (see Supplemental Figures 1A to 1E online). From these ...
... MTs using the drug jasplakinolide (Bubb et al., 1994). Because jasplakinolide has not been extensively employed in plant cell biology, we first grew Arabidopsis seedlings on different concentrations of the drug and assessed the impact on growth (see Supplemental Figures 1A to 1E online). From these ...
PAK1 Mediates Resistance to PI3 Kinase Inhibition in Lymphomas
... Gene expression profiling utilizing Affymetrix Gene 1.0 ST arrays were performed, scanned, and normalized as previously described (17). These data are available through Gene Expression Omnibus (GEO). The microarray data will be available through accession number:GSE22898. The gene expression data fo ...
... Gene expression profiling utilizing Affymetrix Gene 1.0 ST arrays were performed, scanned, and normalized as previously described (17). These data are available through Gene Expression Omnibus (GEO). The microarray data will be available through accession number:GSE22898. The gene expression data fo ...
Resident in Normal Skin T Cells Are + The Vast Majority of CLA
... method involves culture of skin explants on three-dimensional matrices that induce the ingrowth of dermal fibroblasts. Chemokines produced by these fibroblasts induce the migration of T cells out of explants, where they can be collected and studied. Although this method requires a culture period, it ...
... method involves culture of skin explants on three-dimensional matrices that induce the ingrowth of dermal fibroblasts. Chemokines produced by these fibroblasts induce the migration of T cells out of explants, where they can be collected and studied. Although this method requires a culture period, it ...
AMPK regulates ER morphology and function in
... formation of cisternae, although some of the tubule-shaping proteins may also be involved in stabilizing the edges of these structures (10). The ER and mitochondria exhibit tightly coupled dynamics and have extensive contacts. A recent report suggested that ER tubules define the position of mitochon ...
... formation of cisternae, although some of the tubule-shaping proteins may also be involved in stabilizing the edges of these structures (10). The ER and mitochondria exhibit tightly coupled dynamics and have extensive contacts. A recent report suggested that ER tubules define the position of mitochon ...
Cell-penetrating peptides and antimicrobial peptides: how different
... Pep-1 has been used to introduce large proteins inside cell lines [57–59]. An endosomal pathway was rejected because (1) there was no difference in translocation efficiency at 37 ◦C and 4 ◦C [57] and (2) no co-localization of a delivered protein (β-galactosidase from Escherichia coli) with different ...
... Pep-1 has been used to introduce large proteins inside cell lines [57–59]. An endosomal pathway was rejected because (1) there was no difference in translocation efficiency at 37 ◦C and 4 ◦C [57] and (2) no co-localization of a delivered protein (β-galactosidase from Escherichia coli) with different ...
Biochemistry of Fruits and Vegetables
... Freeze-thaw cycles: Commodity specific, but membrane damage/rupturing is considerably greater with a slow freeze (large ice crystals) compared to a rapid freeze. Cryoprotective agents can be used to protect the cells if necessary; examples include sugar or salt solutions (increases the osmotic press ...
... Freeze-thaw cycles: Commodity specific, but membrane damage/rupturing is considerably greater with a slow freeze (large ice crystals) compared to a rapid freeze. Cryoprotective agents can be used to protect the cells if necessary; examples include sugar or salt solutions (increases the osmotic press ...
Actin microfilaments in fungi
... which are thought to function in pulling the plasma membrane inwards at the end of mitosis, thus facilitating septum deposition (which is considered in more detail below). It is of interest to note that the actin cytoskeleton of the other group of hyphal organisms, the oomycetes differs somewhat fro ...
... which are thought to function in pulling the plasma membrane inwards at the end of mitosis, thus facilitating septum deposition (which is considered in more detail below). It is of interest to note that the actin cytoskeleton of the other group of hyphal organisms, the oomycetes differs somewhat fro ...
blood pressure
... occurs by diffusion across the gill surfaces, and fluid in the coelom circulates in and out of the gills, aiding gas transport. The surfaces of a sea star’s tube feet also function in gas exchange. ...
... occurs by diffusion across the gill surfaces, and fluid in the coelom circulates in and out of the gills, aiding gas transport. The surfaces of a sea star’s tube feet also function in gas exchange. ...
CLC Chloride Channels in Caenorhabditis elegans*
... CeCLC-3 gave larger, strongly inwardly rectifying currents, which activated rapidly (t ; 10 ms) upon hyperpolarization (Fig. 3C). This was followed by a slower (t ; 500 ms) decay to stationary values after a maximum at ;80 ms (Fig. 3, D and E). Similar results were obtained in the HEK293 expression ...
... CeCLC-3 gave larger, strongly inwardly rectifying currents, which activated rapidly (t ; 10 ms) upon hyperpolarization (Fig. 3C). This was followed by a slower (t ; 500 ms) decay to stationary values after a maximum at ;80 ms (Fig. 3, D and E). Similar results were obtained in the HEK293 expression ...
PDF
... Early kidney development is characterized by branching of the ureteric bud epithelium and differentiation of the metanephrogenic mesenchyme surrounding the ureteric bud into epithelial kidney tubules. These synchronous events are regulated by interactions between the ureteric epithelium and the mese ...
... Early kidney development is characterized by branching of the ureteric bud epithelium and differentiation of the metanephrogenic mesenchyme surrounding the ureteric bud into epithelial kidney tubules. These synchronous events are regulated by interactions between the ureteric epithelium and the mese ...