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starry night regulates tissue polarity - Development
starry night regulates tissue polarity - Development

... slightly weaker than stan3/Df-stan wings (compare Fig. 1G and H), thus by this criteria stan3 is a strong hypomorphic allele for the wing tissue polarity phenotype. Since stan3 is healthy, the mutation is presumably functional for the stan vital functions. It is possible that stan3 is analogous to t ...
the diversity of plastid - University of Wisconsin Oshkosh
the diversity of plastid - University of Wisconsin Oshkosh

... There are three evolutionary lines of plastids: the glaucophytes, the red lineage and the green lineage. The glaucophyte line of chloroplasts is represented by the muroplasts, primitive walled chloroplasts found in the glaucocystophytic algae. Rhodoplasts represent the red lineage and are the chloro ...
Immune complexes formed following the binding of
Immune complexes formed following the binding of

... arterial and venular circulation. This can lead to limb- and life-threatening complications. Although the precise mechanism(s) of thrombosis is yet unestablished, evidence in literature supports a role for activated platelets, resulting procoagulants, and microparticles.13 Immune complexes also bind ...
immunodetection of arabinogalactan proteins in different types of
immunodetection of arabinogalactan proteins in different types of

... of Oe. mut brevistylis were selected according to morphological features from the progeny of a fertile plant. Flowers of Galanthus nivalis were received from the Botanical Garden of Maria Curie-Skłodowska University in Lublin. The selected plants were isolated with bags to prevent uncontrolled polli ...
Neural differentiation from embryonic stem cells in vitro: An overview
Neural differentiation from embryonic stem cells in vitro: An overview

... reported the differentiation of ESCs into a range of embryonic tissues. These compelling results were done either by stimulating the cells with particular molecules or by simulating the environmental cues of the early embryo. Importantly, these differentiated cells can then be used in regenerative ...
Progress in understanding the role of microtubules in plant cells
Progress in understanding the role of microtubules in plant cells

... membranous inclusions, nucleotides and ions. Some residents, the microtubule-associated proteins and their regulatory elements, co-ordinate the assembly, bundling and stability of the microtubules. Others are motor proteins that shuttle cargo or participate in microtubule organization. Perhaps hundr ...
Morphogenesis of complex plant cell shapes: the mechanical role of
Morphogenesis of complex plant cell shapes: the mechanical role of

... Cellulase affects pollen tube germination and growth differently in Lilium and Solanum In order to germinate, the cell wall at the pollen grain aperture yields in relationship to the pressure generated by water uptake into the hydrating cell. The same mechanical principle applies to pollen tube elon ...
Chromatin folding – from biology to polymer models and back
Chromatin folding – from biology to polymer models and back

... experimentally and their outcome can be used to further improve the model, thereby increasing our understanding of chromatin folding. In this Commentary, we demonstrate that efforts to unravel the complex and dynamic relationship between eukaryotic gene regulation and chromatin folding benefit from ...
Negative feedback control of the autoimmune
Negative feedback control of the autoimmune

... dose of i.n. peptide 2 h before PMA and ionomycin stimulation in vitro (unpublished data). No IL-17 was detected in CD4+ T cells from either untreated or peptide-treated mice (Fig. 3 A), suggesting that the IL-17 detected in serum (Fig. 1) originated from a different cellular source, such as NKT cel ...
Automatic sampling for unbiased and efficient stereological
Automatic sampling for unbiased and efficient stereological

... The correlation between the biased weight of a field of view and the correct count in the field may be positive or negative. Regardless of that, the estimate is always unbiased. The precision (CE) is, however, much dependent on the relationship between weight and count: the more positive the better ...
RalA ACTIVATION ASSAY BIOCHEM KIT
RalA ACTIVATION ASSAY BIOCHEM KIT

... When possible, the untreated samples should have cellular levels of RalA activity in a “controlled state”. For example, when looking for RalA activation the “controlled state” cells could be serum starved. Serum starvation will inactivate cellular RalA and lead to a much greater response to a given ...
SOMBRERO, BEARSKIN1, and BEARSKIN2 Regulate Root Cap
SOMBRERO, BEARSKIN1, and BEARSKIN2 Regulate Root Cap

... different LRC morphology to other plant species, in which the cells in a LRC layer remain joined to each other, even when the layer detaches from the root itself; in other species, border cells are released individually from the root cap (Vicré et al., 2005). The functional significance of this alt ...
phosphatases and differentiation of the golgi apparatus
phosphatases and differentiation of the golgi apparatus

... zone' (Clowes, 1961), mitotic activity is apparently much lower than in some of the other cell types of the root, as are certain other metabolic activities (Jensen, 1957, 1958). Just basipetal to this region the cells, here termed apical initials, have a higher rate of mitotic activity, but are not ...
Microscopic diffusion anisotropy in formalin fixed prostate tissue
Microscopic diffusion anisotropy in formalin fixed prostate tissue

... in vivo at spatial resolutions where the voxel volume is typically similar to the tissue sample size used in this study (28 mm3). It is possible that microscopic heterogeneity of stromal fiber direction has contributed to diverse findings from attempts to measure FA in the prostate in vivo (8–12). H ...
Physiology
Physiology

... – Function almost instantaneously – Cells that are utilizing O2, produce CO2, which builds up. Thus, more CO2 is found in the tissue cells than in nearby blood cells. This results in a pressure (pCO2). – Diffusion occurs, the CO2 leaves the tissue through the interstitial fluid into the capillary bl ...
The Immune System
The Immune System

... On the whole, your immune system does a remarkable job of defending you against disease-causing microorganisms. But sometimes it fails: A germ invades successfully and makes you sick. Is it possible to intervene in this process and make your immune system stronger? What if you improve your diet? Ta ...
The nucleolus through the years
The nucleolus through the years

... HCl in Feulgen procedure or before staining with basic dyes also remove RNA in examined specimens. The functions of the nucleolus were mysterious for a long period of time even after structural and morphological definition of main nucleolar components. However, the different nucleolar size in prolif ...
Differentiation-associated changes in CD44 isoform expression
Differentiation-associated changes in CD44 isoform expression

... (HS) at room temperature to block Fc receptors, then washed twice with HFN, and incubated with an anti-CD44 MoAb (3C12, 2G1) or the IgGl control MoAb for 30 minutes at 4"C, followed by two washes with HFN. Samples were then resuspended in a 150 dilution of GAM-FITC in HFN, incubated for 30 minutes a ...
Remodeling of endosomes during lysosome biogenesis involves
Remodeling of endosomes during lysosome biogenesis involves

... doi:10.1242/jcs.00259 ...
Necrotizing Enterocolitis: Research Agenda for a Disease
Necrotizing Enterocolitis: Research Agenda for a Disease

... The pathology of NEC demonstrates mucosal and transmural necrosis and inflammation (1, 2). There are associated mucosal ulceration, hemorrhage, and edema in most patients, whereas eosinophilic infiltration and crypt abscesses are noted less frequently. The terminal ileum and proximal colon are most ...
TMBP200, a Microtubule Bundling Polypeptide Isolated from
TMBP200, a Microtubule Bundling Polypeptide Isolated from

... et al. 1995). As the formation of the cell plate progresses, microtubules in the central region of the phragmoplast depolymerize and the phragmoplast assumes a ring-like structure, expanding outwards with the centrifugal growth of the cell plate. The phragmoplast expands by polymerization of microtu ...
Insert Title Here
Insert Title Here

... Q. Water enters the outermost cells of the root by osmosis. What does this tell you about the cell sap of these outermost cells? Active transport ...
Chapter 06 (Urinalyisis
Chapter 06 (Urinalyisis

... of white blood cells either as whole cells or as lysed cells. • Pyuria can be detected even if the urine sample contains damaged or lysed WBC's. • A negative leukocyte esterase test means that an infection is unlikely and that, without additional evidence of urinary tract infection, microscopic exam ...
Lipid-like materials for low-dose, in vivo gene silencing Please share
Lipid-like materials for low-dose, in vivo gene silencing Please share

... lipid-like compounds based on epoxide chemistry. The library is composed of nondegradable amino alcohols consisting of polar amine-containing head groups and nonpolar hydrocarbon tails. Synthesis of the compounds was achieved through efficient ring-opening of epoxides by amine substrates (Fig. 1). T ...
Name Notes Page ______ 1 Bacteria Objectives
Name Notes Page ______ 1 Bacteria Objectives

... autotroph. Other bacterial cells, which must “eat” to survive are called heterotrophs. This is true for all organisms in all kingdoms. What type of cell are bacteria? Prokaryotic How does the bacterial cell compare in size with other cells? It is much smaller. Why are bacteria considered the simples ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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