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... destabilize the mRNA. This destabilization is counteracted by p38MAPK activity (21, 22). Thus, Hro-notch mRNA, which also bears class 2 AREs in its 3⬘ UTR, is a strong candidate for p38MAPK-regulated transcript stability. To determine whether p38MAPK is activated in the two-cell leech embryo, we imm ...
... destabilize the mRNA. This destabilization is counteracted by p38MAPK activity (21, 22). Thus, Hro-notch mRNA, which also bears class 2 AREs in its 3⬘ UTR, is a strong candidate for p38MAPK-regulated transcript stability. To determine whether p38MAPK is activated in the two-cell leech embryo, we imm ...
NORDINImmunomodulatoryEffects2012 - QMRO Home
... succeeded in identifying two compounds 6-shogaol (6S) and 1-dehydro-6gingerdione (GD) with potent inhibitory effects on NO and PGE 2. These effects were comparable to dexamethasone and indomethacin. ...
... succeeded in identifying two compounds 6-shogaol (6S) and 1-dehydro-6gingerdione (GD) with potent inhibitory effects on NO and PGE 2. These effects were comparable to dexamethasone and indomethacin. ...
Protein sorting at the ER–Golgi interface
... Coat adaptors recognize sorting signals.Studies on the internalization of cell surface receptors via clathrin-mediated endocytosis first established the principle that specific protein-based signals mediate capture of cargo into vesicles. Subsequent biochemical, structural, and genetic dissection o ...
... Coat adaptors recognize sorting signals.Studies on the internalization of cell surface receptors via clathrin-mediated endocytosis first established the principle that specific protein-based signals mediate capture of cargo into vesicles. Subsequent biochemical, structural, and genetic dissection o ...
Characterising Non-Structural Protein NS4 of African Horse Sickness Virus
... Wax-embedded heart, lung and spleen tissues were obtained from AHSV-positive and-negative horses. The AHSV-positive horses presented for routine diagnostic necropsy at the Veterinary Pathology Reference Laboratory (University of Pretoria). The one horse was a one year old cross-Arabian gelding natur ...
... Wax-embedded heart, lung and spleen tissues were obtained from AHSV-positive and-negative horses. The AHSV-positive horses presented for routine diagnostic necropsy at the Veterinary Pathology Reference Laboratory (University of Pretoria). The one horse was a one year old cross-Arabian gelding natur ...
Epithelial enhancement of connective tissue
... Somites isolated from each embryo were cut into sections for explant cultures, such that each piece contained three somites on either side of the central notochord. The notochord was removed before culturing for explants without notochord. The explants were divided into three groups: one group conta ...
... Somites isolated from each embryo were cut into sections for explant cultures, such that each piece contained three somites on either side of the central notochord. The notochord was removed before culturing for explants without notochord. The explants were divided into three groups: one group conta ...
12 October 2000
... Caspase-mediated cleavage of specific substrates also explains several of the other characteristic features of apoptosis. For example, cleavage of the nuclear lamins is required for nuclear shrinking and budding15, 16. Loss of overall cell shape is probably caused by the cleavage of cytoskeletal pr ...
... Caspase-mediated cleavage of specific substrates also explains several of the other characteristic features of apoptosis. For example, cleavage of the nuclear lamins is required for nuclear shrinking and budding15, 16. Loss of overall cell shape is probably caused by the cleavage of cytoskeletal pr ...
Blood First Edition Paper, prepublished online September 13, 2012
... The thrombin species produced in Tf-activated whole blood were quantitated using immunoassays which utilize monoclonal antibodies to αIIa (αTAT) (βTAT and γTAT are also recognized) or prothrombin fragment 2 (mTAT) to capture their respective antigens. Standards were prepared by reacting human αIIa o ...
... The thrombin species produced in Tf-activated whole blood were quantitated using immunoassays which utilize monoclonal antibodies to αIIa (αTAT) (βTAT and γTAT are also recognized) or prothrombin fragment 2 (mTAT) to capture their respective antigens. Standards were prepared by reacting human αIIa o ...
Regulators of Lysosome Function and Dynamics in Caenorhabditis
... biosynthetic pathway to lysosomes, a process referred to as lysosome biogenesis (Mullins and Bonifacino 2001; Luzio et al. 2003). Much remains to be discovered about molecular regulation of these complex fusion, fission, and reformation reactions in the late endocytic pathway. We had made Pmyo-3::ssG ...
... biosynthetic pathway to lysosomes, a process referred to as lysosome biogenesis (Mullins and Bonifacino 2001; Luzio et al. 2003). Much remains to be discovered about molecular regulation of these complex fusion, fission, and reformation reactions in the late endocytic pathway. We had made Pmyo-3::ssG ...
The linkage between cell wall metabolism and fruit
... Contract/grant sponsor: Fulbright Commission (Received 31 August 2006; accepted 25 October 2006) Published online 2 April 2007; DOI: 10.1002/jsfa.2837 ...
... Contract/grant sponsor: Fulbright Commission (Received 31 August 2006; accepted 25 October 2006) Published online 2 April 2007; DOI: 10.1002/jsfa.2837 ...
Mice lacking synaptophysin reproduce and form typical synaptic
... either normal mice or neomycin-resistant transgenic mice (kindly provided by the animal facility of the EMBL, Heidelberg, Germany) under standard conditions (Nagy et al. 1993; Torres and Mansouri 1994) in the presence of 1000 U/ml leukemia inhibitory factor (ESGRO, Gibco BRL, Gaithersburg, Md.). Lin ...
... either normal mice or neomycin-resistant transgenic mice (kindly provided by the animal facility of the EMBL, Heidelberg, Germany) under standard conditions (Nagy et al. 1993; Torres and Mansouri 1994) in the presence of 1000 U/ml leukemia inhibitory factor (ESGRO, Gibco BRL, Gaithersburg, Md.). Lin ...
DC-derived exosomes bear molecules involved in
... hybrids was performed by incubation of graded numbers of (B10) BMDC with 105 hybrid cells/well in flat-bottom, 96 well plates. For blocking experiments, BMDC were pretreated for 30 min with 30µg/ml of Y-Ae mAb or irrelevant IgG2b before adding the T cells. Twenty four h later, 50 µl aliquots of supe ...
... hybrids was performed by incubation of graded numbers of (B10) BMDC with 105 hybrid cells/well in flat-bottom, 96 well plates. For blocking experiments, BMDC were pretreated for 30 min with 30µg/ml of Y-Ae mAb or irrelevant IgG2b before adding the T cells. Twenty four h later, 50 µl aliquots of supe ...
spleen-facilitated vesiculation Hemoglobin loss
... respectively (Table 3). From these comparisons it can be concluded that the hemoglobin composition of vesicles closely resembles that of old RBCs. Figure 2. Freeze-fracture electron microscopy of vesicles. Vesicles were isolated from plasma obtained by pheresis from a healthy blood bank donor. The s ...
... respectively (Table 3). From these comparisons it can be concluded that the hemoglobin composition of vesicles closely resembles that of old RBCs. Figure 2. Freeze-fracture electron microscopy of vesicles. Vesicles were isolated from plasma obtained by pheresis from a healthy blood bank donor. The s ...
Long-Term Acclimation of the Cyanobacterium
... HL2, 300 mE m22 s21). Absorption spectra of cells normalized per optical density at 750 nm (OD 750) are shown in Figure 1A. Whereas the shift to LL did not result in significant changes in the cell pigmentation (data not shown; see Table I for Chl level), amounts of Chl and phycobilisomes per cell we ...
... HL2, 300 mE m22 s21). Absorption spectra of cells normalized per optical density at 750 nm (OD 750) are shown in Figure 1A. Whereas the shift to LL did not result in significant changes in the cell pigmentation (data not shown; see Table I for Chl level), amounts of Chl and phycobilisomes per cell we ...
Ocular Anatomy and Retinal Photoreceptors in a Skink, the Sleepy
... their inner segments in broad contiguity though kept separate by their cell membranes (Fig. 7B). The two members of these pairings were morphologically different; one member short and broad containing a prominent paraboloid body and the second member long and narrow with a small or absent paraboloid ...
... their inner segments in broad contiguity though kept separate by their cell membranes (Fig. 7B). The two members of these pairings were morphologically different; one member short and broad containing a prominent paraboloid body and the second member long and narrow with a small or absent paraboloid ...
Photoactivation of GFP reveals protein dynamics within the
... morphology such that flat sheet-like regions of ER were observed (Fig. 1b). Sheets of ER were connected by tubules that appeared similar to those of the normally reported ER network. Because the ER is not visible prior to photoactivation of the PAGFP in its membrane, only about 75% of the cells that ...
... morphology such that flat sheet-like regions of ER were observed (Fig. 1b). Sheets of ER were connected by tubules that appeared similar to those of the normally reported ER network. Because the ER is not visible prior to photoactivation of the PAGFP in its membrane, only about 75% of the cells that ...
From the regulation of peptidoglycan synthesis to bacterial growth
... penicillin-binding proteins (PBPs), were initially identified because of their ability to covalently bind penicillin8. There are three types of these peptidoglycan synthases: bifunctional GTase–TPases (the class A PBPs), monofunctional TPases (the class B PBPs) and monofunctional GTases7. E. coli ha ...
... penicillin-binding proteins (PBPs), were initially identified because of their ability to covalently bind penicillin8. There are three types of these peptidoglycan synthases: bifunctional GTase–TPases (the class A PBPs), monofunctional TPases (the class B PBPs) and monofunctional GTases7. E. coli ha ...
Vacuolar Sorting Receptor-Mediated Trafficking of Soluble Vacuolar
... types of vacuoles exist in plant cells: the lytic vacuole in vegetative cells and the protein storage vacuole (PSV) in seed cells [1,2]. These vacuoles play many important roles, such as the degradation of waste materials, ion and metabolite storage, and the maintenance of turgor pressure. To perfor ...
... types of vacuoles exist in plant cells: the lytic vacuole in vegetative cells and the protein storage vacuole (PSV) in seed cells [1,2]. These vacuoles play many important roles, such as the degradation of waste materials, ion and metabolite storage, and the maintenance of turgor pressure. To perfor ...
Full Text - International Journal of Livestock Research
... to be a valuable animal model for insights into immune function. In particular, the development of B cells in this unique organ, the bursa of Fabricius, has provided a novel opportunity to study B cell development (Funk and Thompson, 1996). The bursa also functions as a peripheral gut-associated lym ...
... to be a valuable animal model for insights into immune function. In particular, the development of B cells in this unique organ, the bursa of Fabricius, has provided a novel opportunity to study B cell development (Funk and Thompson, 1996). The bursa also functions as a peripheral gut-associated lym ...
Bacterial Growth and Cell Division: a Mycobacterial Perspective
... factor), sulfolipids specific to M. tuberculosis, and the phosphatidylinositol mannosides. In slow-growing, pathogenic mycobacteria, such as M. tuberculosis and M. leprae, the LAMs are capped at the terminal -Ara residue with mannose residues and are referred to as ManLAMs (68, 69, 301, 324), where ...
... factor), sulfolipids specific to M. tuberculosis, and the phosphatidylinositol mannosides. In slow-growing, pathogenic mycobacteria, such as M. tuberculosis and M. leprae, the LAMs are capped at the terminal -Ara residue with mannose residues and are referred to as ManLAMs (68, 69, 301, 324), where ...
Publizieren ist ein essentieller Bestandteil des wissenschaftlichen
... We anticipate our assay to be a starting point for more sophisticated in vitro models of mitotic spindles. For example, the individual and combined action of multiple mitotic motors could be tested, including minus-end-directed motors opposing Eg5 motility. Furthermore, Eg5 inhibition is a major tar ...
... We anticipate our assay to be a starting point for more sophisticated in vitro models of mitotic spindles. For example, the individual and combined action of multiple mitotic motors could be tested, including minus-end-directed motors opposing Eg5 motility. Furthermore, Eg5 inhibition is a major tar ...
O A RIGINAL RTICLE
... is synthesized and secreted by mucous neck cells in the stomach and goblet cells in the intestine [31]. Mucin is made up of fucose, galactose, Nacetylglucosamine, N-acetylgalactosamine and SA. The SA usually occupy the terminal position of the oligosaccharide chain of the glycoconjugate and these te ...
... is synthesized and secreted by mucous neck cells in the stomach and goblet cells in the intestine [31]. Mucin is made up of fucose, galactose, Nacetylglucosamine, N-acetylgalactosamine and SA. The SA usually occupy the terminal position of the oligosaccharide chain of the glycoconjugate and these te ...
Equine Herpesvirus Type 1: Immune Evasion and - diss.fu
... 2.3.1 Cells and viruses............................................................................................................ 34 2.3.2 Antibodies ..................................................................................................................... 34 2.3.3 Viral mutagenesis ... ...
... 2.3.1 Cells and viruses............................................................................................................ 34 2.3.2 Antibodies ..................................................................................................................... 34 2.3.3 Viral mutagenesis ... ...
comparison of tgf/bmp superfamily pathways signaled by bmp
... kinase inhibitor P21/Waf1/Cip1 by 160%, whereas it was decreased (30%) by rhBMP -2. It is possible that DBP's inhibition of proliferation may contribute to its effects to promote differentiation. Cbfa1 was highly expressed in target hDFs but was moderately decreased by both DBP (10%) and rhBMP -2 (2 ...
... kinase inhibitor P21/Waf1/Cip1 by 160%, whereas it was decreased (30%) by rhBMP -2. It is possible that DBP's inhibition of proliferation may contribute to its effects to promote differentiation. Cbfa1 was highly expressed in target hDFs but was moderately decreased by both DBP (10%) and rhBMP -2 (2 ...