Classification - HAL
... = 34 vs 119 nM). In contrast, displacements of 35S-gp120Bx08 binding by the agonists CCL3 (Fig. 1C), CCL4 and PSC-RANTES (Fig. 1D) gave biphasic curves, consistent with the presence of two distinct 35S-gp120Bx08 receptor populations, one with high affinity for these chemokines, the other with signif ...
... = 34 vs 119 nM). In contrast, displacements of 35S-gp120Bx08 binding by the agonists CCL3 (Fig. 1C), CCL4 and PSC-RANTES (Fig. 1D) gave biphasic curves, consistent with the presence of two distinct 35S-gp120Bx08 receptor populations, one with high affinity for these chemokines, the other with signif ...
mTORC1 and the regulation of skeletal muscle anabolism and mass
... Two important factors that regulate muscle mass and function are nutrition and physical activity. Compared with other macronutrients, proteins and branched-chain amino acids have pronounced effects in stimulating muscle protein anabolism (Kimball and Jefferson 2010; Little and Phillips 2009). The an ...
... Two important factors that regulate muscle mass and function are nutrition and physical activity. Compared with other macronutrients, proteins and branched-chain amino acids have pronounced effects in stimulating muscle protein anabolism (Kimball and Jefferson 2010; Little and Phillips 2009). The an ...
Maltose uptake and its regulation in Bacillus subtilis
... both the wild-type and PG587 accumulated maltose to a comparable degree when assayed at 37°C, only the wild-type accumulated the sugar at 47°C (Table 1). The most probable conclusion from these experiments is that maltose transport, although non-PTS, is regulated by the PTS in B. ...
... both the wild-type and PG587 accumulated maltose to a comparable degree when assayed at 37°C, only the wild-type accumulated the sugar at 47°C (Table 1). The most probable conclusion from these experiments is that maltose transport, although non-PTS, is regulated by the PTS in B. ...
Allosteric Interactions after 50Years
... units as the basis of allostery. A number of novel equations and useful concepts (such as the “allosteric range”) were also presented in their long-latent text now published in this issue for the first time. 28 For one particularly valuable equation relating the Hill coefficient and the number of bi ...
... units as the basis of allostery. A number of novel equations and useful concepts (such as the “allosteric range”) were also presented in their long-latent text now published in this issue for the first time. 28 For one particularly valuable equation relating the Hill coefficient and the number of bi ...
Isoprenoid biosynthesis in bacterial pathogens
... presence of the mevalonate pathway genes. Wilding et al. (2000a) verified the essential requirement of the mevalonate pathway gene mvaA, which encodes the well-characterized and rate-limiting enzyme HMG-CoA reductase (Bochar et al., 1999). Initial attempts to delete this gene were unsuccessful, and ...
... presence of the mevalonate pathway genes. Wilding et al. (2000a) verified the essential requirement of the mevalonate pathway gene mvaA, which encodes the well-characterized and rate-limiting enzyme HMG-CoA reductase (Bochar et al., 1999). Initial attempts to delete this gene were unsuccessful, and ...
Auxin and other signals on the move in plants Auxins are a class of
... repressors (the Aux/IAA repressors) for ubiquitination by the SCF complex. This marking process leads to the degradation of the repressors by the proteasome, alleviating repression and leading to expression of specific genes in response to auxins (reviewed in Another protein called ABP1 (Auxin Bindi ...
... repressors (the Aux/IAA repressors) for ubiquitination by the SCF complex. This marking process leads to the degradation of the repressors by the proteasome, alleviating repression and leading to expression of specific genes in response to auxins (reviewed in Another protein called ABP1 (Auxin Bindi ...
Profilin association with monomeric actin in
... Fractionation of cell extracts About 10 g of amoebas from 1 liter log-phase cultures in Neff’s medium in shaken Fernbach flasks were washed with 50 mM KCl at 22°C, mixed with 10 ml of ice-cold extraction buffer and lysed with 18 strokes of a Dounce homogenizer. Separate 1.0 ml samples of the crude c ...
... Fractionation of cell extracts About 10 g of amoebas from 1 liter log-phase cultures in Neff’s medium in shaken Fernbach flasks were washed with 50 mM KCl at 22°C, mixed with 10 ml of ice-cold extraction buffer and lysed with 18 strokes of a Dounce homogenizer. Separate 1.0 ml samples of the crude c ...
Full Text - The International Journal of Developmental Biology
... Interest in the Spemann-Mangold experiment has endured for 75 years because the organizer is crucial for patterning the chordate body axis and because induction is a ubiquitous element of mechanisms of cellular determination in metazoa. The organizer, which includes approximately 10% of the cells of ...
... Interest in the Spemann-Mangold experiment has endured for 75 years because the organizer is crucial for patterning the chordate body axis and because induction is a ubiquitous element of mechanisms of cellular determination in metazoa. The organizer, which includes approximately 10% of the cells of ...
fuel cell power for vehicles - University of Michigan Transportation
... About 60 million new cars are sold worldwide each year. Several automotive industry leaders have speculated that fuel cell vehicles could account for 20 to 25 percent of new car sales within the next 20 to 25 years, a potential market of 12 million to 15 million vehicles each year. Billions of dolla ...
... About 60 million new cars are sold worldwide each year. Several automotive industry leaders have speculated that fuel cell vehicles could account for 20 to 25 percent of new car sales within the next 20 to 25 years, a potential market of 12 million to 15 million vehicles each year. Billions of dolla ...
A Journey from the Pool of Chiral Synthetic Building Blocks to Cell
... results are published in ca. 20 papers.[29c] The β-peptidyl aminopeptidase BapA belongs to the well-known group of so-called N-terminal nucleophile (Ntn) hydrolases, only five of which have now been identified as β-amino-peptidases. Our BapA enzyme has the peculiar property of accepting all N-termin ...
... results are published in ca. 20 papers.[29c] The β-peptidyl aminopeptidase BapA belongs to the well-known group of so-called N-terminal nucleophile (Ntn) hydrolases, only five of which have now been identified as β-amino-peptidases. Our BapA enzyme has the peculiar property of accepting all N-termin ...
Plasma membrane microdomains from hybrid aspen cells are
... cells, suggesting that their association with DRMs is a general feature of higher plants. Our ...
... cells, suggesting that their association with DRMs is a general feature of higher plants. Our ...
Therapeutic targeting of autophagy in neurodegenerative and
... which produces phosphatidylinositol 3-phosphate (PI3P); this, in turn, helps to recruit ATG16L1 to sites of autophagosome formation (Dooley et al., 2014). Some of these signals act via the ATG6 orthologue Beclin 1, which stimulates VPS34 activity (Furuya et al., 2005; Russell et al., 2013). However, ...
... which produces phosphatidylinositol 3-phosphate (PI3P); this, in turn, helps to recruit ATG16L1 to sites of autophagosome formation (Dooley et al., 2014). Some of these signals act via the ATG6 orthologue Beclin 1, which stimulates VPS34 activity (Furuya et al., 2005; Russell et al., 2013). However, ...
Amino acids and mTORC1: from lysosomes to disease
... several cellular anabolic processes, such as mRNA translation and ribosome biogenesis, lipid synthesis, whereas it blocks autophagy and other catabolic processes. mTORC1 activation also unleashes a negative feedback loop to the insulin receptor, which tends to dampen insulin/PI3K (phosphatidylinosit ...
... several cellular anabolic processes, such as mRNA translation and ribosome biogenesis, lipid synthesis, whereas it blocks autophagy and other catabolic processes. mTORC1 activation also unleashes a negative feedback loop to the insulin receptor, which tends to dampen insulin/PI3K (phosphatidylinosit ...
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... For quite some decades after, slime was no object of research. The next serious record was the For quite some decades after, slime was no object of research. The next serious record was publication of Beckwith from 1931 [4], describing slime as a nuisance in paper production. He the publication of B ...
... For quite some decades after, slime was no object of research. The next serious record was the For quite some decades after, slime was no object of research. The next serious record was publication of Beckwith from 1931 [4], describing slime as a nuisance in paper production. He the publication of B ...
Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as
... characterized. A widely accepted model for ER-to-Golgi transport is based on the sequential action of COPII and COPI coat complexes. The COPII complex assembles by the ordered recruitment of cytosolic components on the ER membrane. Here, we have visualized two early components of the COPII machinery ...
... characterized. A widely accepted model for ER-to-Golgi transport is based on the sequential action of COPII and COPI coat complexes. The COPII complex assembles by the ordered recruitment of cytosolic components on the ER membrane. Here, we have visualized two early components of the COPII machinery ...
Pax proteins and eye development Rachel Macdonald 1 and
... tell us anything about Pax-6 function at later stages of development. Heterozygotes do, however, exhibit eye defects that may shed light on later aspects of Pax-6 function. These defects must result from reduced levels of Pax-6 either during development or within cells that constitutively express th ...
... tell us anything about Pax-6 function at later stages of development. Heterozygotes do, however, exhibit eye defects that may shed light on later aspects of Pax-6 function. These defects must result from reduced levels of Pax-6 either during development or within cells that constitutively express th ...
Excitatory Mechanisms in the Suprachiasmatic Nucleus: The Role of
... that cells in the SCN continue to show circadian oscillations when isolated from the animal in a brain slice preparation (e.g., Green and Gillette 1982). Accordingly, care must be taken as to the time in the daily cycle when the data are collected. Some of the animals were killed 30 min before the t ...
... that cells in the SCN continue to show circadian oscillations when isolated from the animal in a brain slice preparation (e.g., Green and Gillette 1982). Accordingly, care must be taken as to the time in the daily cycle when the data are collected. Some of the animals were killed 30 min before the t ...
Microtubules
... as well as other proteins, are either present in centrioles or required for their formation. ...
... as well as other proteins, are either present in centrioles or required for their formation. ...
Sanguinarine (Pseudochelerythrine) Is a Potent Inhibitor of NF
... either p50 or p65 subunits of NF-kB for 30 min at 37 °C before the complex was analyzed by EMSA. Antibody against cyclin D1 was included as a negative control. Western Blotting for IkBa and p65—Western blotting was carried out essentially as described previously (23). Briefly, the cytoplasmic extrac ...
... either p50 or p65 subunits of NF-kB for 30 min at 37 °C before the complex was analyzed by EMSA. Antibody against cyclin D1 was included as a negative control. Western Blotting for IkBa and p65—Western blotting was carried out essentially as described previously (23). Briefly, the cytoplasmic extrac ...
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... to investigate the intrinsic properties of isolated proteins, via molecular biology, biochemical and structural approaches [1–3]. However, it is also necessary to study protein function in an integrated system in order to understand the phenomena in cellular and physiological contexts. Energy metabo ...
... to investigate the intrinsic properties of isolated proteins, via molecular biology, biochemical and structural approaches [1–3]. However, it is also necessary to study protein function in an integrated system in order to understand the phenomena in cellular and physiological contexts. Energy metabo ...