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molecular cloning and expression of the proliferating cell nuclear
molecular cloning and expression of the proliferating cell nuclear

... gene sequences and protein structures are highly conserved (Kelman 1997). PCNA is required for DNA replication (Prelich et al. 1987) and repair (Celis and Madsen 1986, Li et al. 1994). The function as an accessory factor for DNA replication has been elucidated for organisms, including higher plants ...
BDV
BDV

... ---Membrane proteins and immune response BDV enters cells by plasma membrane fusion. Remarkably, it replicates in the nucleus of the infected cell, requiring nucleocytoplasmic trafficking of BDV macromolecules. As these processes intimately involve the plasma and nuclear membranes, changes in memb ...
Electrochemical model for proton exchange membrane fuel cell
Electrochemical model for proton exchange membrane fuel cell

... Beicha∗, ), [email protected] (Radia Zaamouche) ...
Pdf - MFPL
Pdf - MFPL

... this progresssion, specification occurs: This is the process by which cells in each region of the developing animal come to express a given set of genes. The spatial cues that trigger specification in development are generally signaling ligands produced by other cells, in consequence of their own pr ...
Lab 2 bacteria protists
Lab 2 bacteria protists

... The Archea are probably the least familiar of the two to most people, but include some very important organisms. Prokaryotic thermopiles, which can survive extreme temperatures are used in biotechnology (specifically Polymerase Chain Reaction), while others are responsible for ‘marsh gas” (methane) ...
The Growing Outer Epidermal Wall: Design and
The Growing Outer Epidermal Wall: Design and

... of the hypocotyl an average wall thickness of 0.2 mm was measured. The walls of the pith cells, which are shown in Fig. 2B, had an average thickness of about 0.1 mm. Hence, in light-grown sunflower hypocotyls, the OEW is approximately 20 times thicker than the walls in the centre of the organ. These ...
Activation of the Cell Wall Degrading Protease, Lysin, during Sexual
Activation of the Cell Wall Degrading Protease, Lysin, during Sexual

... of '°60,000 Mr. Fig. 1, a preparative SDS-PAGE gel of lysin purified by ion exchange chromatography (4), shows that the 60,000-Mr polypeptide was the primary constituent of lysin purified by our method. This band was excised from the gel and used as immunogen in a rabbit as described in Materials an ...
Intercellular adhesion and cell separation in plants
Intercellular adhesion and cell separation in plants

... exists for the co-ordinated development of walls of adjacent cells. The absence of shear at the middle lamella is necessary to prevent rupture of plasmodesmatal connections between the adjacent cells during growth. The textured wall structure and patterns of pectic epitopes observed around plasmodes ...
Max and inhibitory c-Myc mutants induce erythroid di
Max and inhibitory c-Myc mutants induce erythroid di

... overexpression of Max results in suppression of cell transformation mediated by c-Myc (Makela et al., 1992; Prendergast et al., 1991; Mukherjee et al., 1992; Amati et al., 1993a; Gu et al., 1993; Koskinen et al., 1994; Lindeman et al., 1995). These data are consistent with the idea that inactive Max ...
Chlorella
Chlorella

... mother cell walls are interspersed with whole cells at a ratio of about 1:4. That is, about 75% of Chlorella divisions are into two daughter cells and 25% into four daughter cells (Fott and Novakova, 1969; Williams, 1971). We have repeatedly observed the same pattern in our Chlorella continuous cult ...
Carbohydrates - Overview
Carbohydrates - Overview

... measure the relative sweetness of simple sugars and artificial sweeteners (figure 2). For example, fructose, which is found in high concentrations in many types of fruit, is perceived to be about three times sweeter than glucose and two times sweeter than the disaccharide sucrose (table sugar). Hone ...
Presentation
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... Membranes contain proteins, the number of proteins varies with cell function Some membrane proteins extend across the lipid bilayer—with hydrophobic and hydrophilic regions or domains. ...
the oncogene of bav-3 as a mutagen
the oncogene of bav-3 as a mutagen

... cell nucleases than intact viral particles. As to the relatively weak effect of the BAV3 oncogene, in this case other factors may also be at play. The most important of these is probably the fact that, while excising the oncogene, the EcoRl endonuclease cuts the enhancer off it. Although the exact l ...
Effects of bvdv on macrophages functions
Effects of bvdv on macrophages functions

... Phagocytic activity: The test was done to determine the effect of BVDV on phagocytic activity of macrophages according to the modified protocol of (Flipo et. al., 1992). Coated beads were diluted in phosphatebuffered saline (PBS) to an appropriate concentration. Macrophages cells in RPMI 1640 medium ...
A New Model of the Human Atrial Myocyte with Variable T
A New Model of the Human Atrial Myocyte with Variable T

... However, currently available mathematical models of human atrial single cell electrophysiology and Ca2+ handling do not explicitly account for variable TT density and organisation. In this study, we develop a new model of the human atrial cell which explicitly accounts spatiotemporal stochastic Ca2+ ...
Evidence for Distinct Amino Acid Transport Systems in
Evidence for Distinct Amino Acid Transport Systems in

... presence of more than one carrier for any one amino acid. M oreover distinct transport systems for neutral, acidic and basic amino acids were kinetically characterized. Based on competition ex­ periments neutral amino acids were absorbed by all transport systems. Aspartic acid entered the cells via ...
Phospholipase D promotes Arcanobacterium haemolyticum
Phospholipase D promotes Arcanobacterium haemolyticum

... pharyngitis by inoculation of bacteria onto the human pharynx were unsuccessful [2]. Intravenous inoculation of A. haemolyticum into rabbits resulted in hemorrhagic pneumonia [2], suggesting this organism can cause invasive disease once it enters the bloodstream. Subsequently, a phospholipase D (PLD ...
Chemical–Biological Studies of Subcellular Organization in Bacteria
Chemical–Biological Studies of Subcellular Organization in Bacteria

... inhibiting FtsZ function, the absence of this phenotype with Z1−Z4 suggests off-target effects on growth or metabolism. No data are available for the binding site or affinity for any of the compounds in this family. Membrane perturbations represent another category of offtarget effects that may be relevan ...
- Wiley Online Library
- Wiley Online Library

... octamer of highly conserved core histones: H2A, H2B, H3 and H4. The binding of linker histone H1 to DNA entry/exit points of nucleosomes and linker DNA between two nucleosomes facilitates further compaction of chromatin into a higher-order structure. The accessibility and compaction of chromatin in ...
Linker histone H1 in early mouse embryogenesis
Linker histone H1 in early mouse embryogenesis

... antibody raised against Xenopus somatic H1 specifically recognized mouse somatic H1 on immunoblot (Fig. 1A, antiH1 panel), and by immunostaining (Fig. 1B, anti-H1 panel), it gave an intense nuclear staining in mouse erythroleukemia (MEL) cells and in mouse blastula cells which is consistent with the ...
Biology Class IX for SA-I 2016-17
Biology Class IX for SA-I 2016-17

... respected sir Shri. K. L. Nagaraju, Retd-AC, KVS RO Bangalore and respected sir Shri M.K. Kulshreshtha, Retd-AC, KVS RO Chandigarh for their blessings, motivation and encouragement in bringing out this project in such an excellent form. I also extend my special thanks to respected sir Shri. P. S. Ra ...
Makorin ortholog LEP-2 regulates LIN-28 stability to
Makorin ortholog LEP-2 regulates LIN-28 stability to

... undifferentiated embryonic stem cells (ESCs) and declines during subsequent development (Thornton and Gregory, 2012; Tsialikas and Romer-Seibert, 2015). LIN-28 inhibits the biogenesis of the mature form of the let-7 microRNA (miRNA) (Newman et al., 2008; Piskounova et al., 2008; Van Wynsberghe et al ...
SALVIA Research Article JIJA MATHEW* AND JOHN E. THOPPIL
SALVIA Research Article JIJA MATHEW* AND JOHN E. THOPPIL

... chromosome aberration assay in spermatocytes. The testes were dissected out from the mice and transferred to 1.2% trisodium citrate solutions. They were washed and transferred to 3 ml of fresh trisodium citrate solution. The tunica layer was removed and tubules of both testes were clubbed together. ...
Hormonal Control of Growth in Plants
Hormonal Control of Growth in Plants

... fruit by treating them with IAA. Since fertilisation has not happened the fruit is formed without a seed! This is called parthenocarpy. Horticulturalists use this to produce a good crop of fruit which all ripen at the same time (to make harvesting more economical). ...
Factor-1-Deficient Mice IL-15-Deficient and IFN Regulatory 3 Cells
Factor-1-Deficient Mice IL-15-Deficient and IFN Regulatory 3 Cells

... NK cells, NK-T cells, and distinct intestinal intraepithelial lymphocyte populations (11, 12). In addition, these studies have revealed a role for IL-15 in the maintenance of the memory CD8⫹ T cell population in the periphery (11). The finding that IL-15⫺/⫺ and IL-15R␣⫺/⫺ mice are lymphopenic sugges ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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