Distributed Processing of Sensory Information
... with the fluorescentdye Lucifer yellow, and drawn usingcamera lucida. A survey of 73 ganglia yielded a population of 235 cells that qualified as dorsal LBIs. Cells of similar soma size, physiological properties (Fig. 2), and dendritic morphology (Fig. 3) were regularly found in similar locations fro ...
... with the fluorescentdye Lucifer yellow, and drawn usingcamera lucida. A survey of 73 ganglia yielded a population of 235 cells that qualified as dorsal LBIs. Cells of similar soma size, physiological properties (Fig. 2), and dendritic morphology (Fig. 3) were regularly found in similar locations fro ...
DRP1 family in cytokinesis and cell expansion
... observed only during the early stages of development and not in mature leaf trichomes (Figure 1E). ADL1E-GUS staining of developing trichomes was restricted to the socket cells of expanding trichomes (Figure 1F). These results suggest that ADL1A function may be more critical than ADL1E function for ...
... observed only during the early stages of development and not in mature leaf trichomes (Figure 1E). ADL1E-GUS staining of developing trichomes was restricted to the socket cells of expanding trichomes (Figure 1F). These results suggest that ADL1A function may be more critical than ADL1E function for ...
Mycobacterium leprae interactions with the host cell: recent
... bacteria can be grown to relatively high concentrations in nine-banded armadillo tissue or in the footpads of nude mice, with the latter system appearing to provide organisms at significantly higher viability levels 20. These techniques have provided highly viable organisms for the M. leprae researc ...
... bacteria can be grown to relatively high concentrations in nine-banded armadillo tissue or in the footpads of nude mice, with the latter system appearing to provide organisms at significantly higher viability levels 20. These techniques have provided highly viable organisms for the M. leprae researc ...
Dialysis Diffusion Lab
... concentration, solute size and the permeability of membranes between the internal and external parts of the cell. The direction of osmosis (water movement across a selectively permeable membrane) depends on the relative concentrations of the cytoplasm and the cell’s surrounding environment. If the c ...
... concentration, solute size and the permeability of membranes between the internal and external parts of the cell. The direction of osmosis (water movement across a selectively permeable membrane) depends on the relative concentrations of the cytoplasm and the cell’s surrounding environment. If the c ...
Salmonella must be viable in order to attach to the
... a wider ongoing study to understand the mechanisms used by bacteria for attachment to tissues. In particular, this paper focuses on the competencies of the organism in the attachment process with a view to exploiting this knowledge to improve postharvest technologies and yield more effective deconta ...
... a wider ongoing study to understand the mechanisms used by bacteria for attachment to tissues. In particular, this paper focuses on the competencies of the organism in the attachment process with a view to exploiting this knowledge to improve postharvest technologies and yield more effective deconta ...
Remodeling of endosomes during lysosome biogenesis involves
... Accepted 6 November 2002 Journal of Cell Science 116, 907-918 © 2003 The Company of Biologists Ltd doi:10.1242/jcs.00259 ...
... Accepted 6 November 2002 Journal of Cell Science 116, 907-918 © 2003 The Company of Biologists Ltd doi:10.1242/jcs.00259 ...
Isoform-Specific Mutations in the Caenorhabditis elegans
... molecular nature of mutations in lin-14 alleles previously analyzed for their effects on temporal cell fates in the lateral hypodermal lineages (Ambros and Horvitz 1987). We were particularly interested in the four partial loss-of-function lin-14b alleles that have L2 stage-specific defects. These m ...
... molecular nature of mutations in lin-14 alleles previously analyzed for their effects on temporal cell fates in the lateral hypodermal lineages (Ambros and Horvitz 1987). We were particularly interested in the four partial loss-of-function lin-14b alleles that have L2 stage-specific defects. These m ...
Local Accumulation of Acetylcholine Receptors Is
... view that electroosmosisplays a significant role in electromigration (Orida and Poo, 1978; McLaughlin and Poo, 1981). Manipulation of electromigration by neuraminidase,in combination with the spatial resolution of the imaging techniques used here, offers a possible meansto separatereceptor accumulat ...
... view that electroosmosisplays a significant role in electromigration (Orida and Poo, 1978; McLaughlin and Poo, 1981). Manipulation of electromigration by neuraminidase,in combination with the spatial resolution of the imaging techniques used here, offers a possible meansto separatereceptor accumulat ...
Copper tolerance of the thermoacidophilic archaeon
... The S. metallicus cells utilized in these experiments were previously adapted to grow at different concentrations of metals. There was a small decrease in the growth rates and the curves reached the plateaus at slightly lower cell densities when the micro-organisms were grown in the presence of incr ...
... The S. metallicus cells utilized in these experiments were previously adapted to grow at different concentrations of metals. There was a small decrease in the growth rates and the curves reached the plateaus at slightly lower cell densities when the micro-organisms were grown in the presence of incr ...
HIV-1 Tropism and Its Detection – Impact on Infection, Transmission
... Angélique van ‘t Wout is a Senior Scientist in the Department of Experimental Immunology, Academic Medical Center, University of Amsterdam. Her interests include the application of genomic technologies to identify the host genetic determinants of HIV-1 susceptibility and disease outcome. Since 1992, ...
... Angélique van ‘t Wout is a Senior Scientist in the Department of Experimental Immunology, Academic Medical Center, University of Amsterdam. Her interests include the application of genomic technologies to identify the host genetic determinants of HIV-1 susceptibility and disease outcome. Since 1992, ...
The Balance between Cell Division and Endoreplication Depends
... plants and recombinant GST or GST-DPB. As shown in Figure 3A, GST-DPB, but not GST alone, interacts with MYC-SKP2A. Furthermore, the amount of MYC-SKP2A recovered in the pull-down experiments increased when ATP was added to the reaction (see Methods), suggesting that a higher phosphorylation of DPB ...
... plants and recombinant GST or GST-DPB. As shown in Figure 3A, GST-DPB, but not GST alone, interacts with MYC-SKP2A. Furthermore, the amount of MYC-SKP2A recovered in the pull-down experiments increased when ATP was added to the reaction (see Methods), suggesting that a higher phosphorylation of DPB ...
Microsoft Word
... most likely due to replication errors [10]. The question arises which factors determine that primary oxidative lesions in mtDNA are converted either to mitochondrial point mutations or to rearrangements of the mitochondrial genome. As reactive oxygen species (ROS) produced in mitochondria can lead t ...
... most likely due to replication errors [10]. The question arises which factors determine that primary oxidative lesions in mtDNA are converted either to mitochondrial point mutations or to rearrangements of the mitochondrial genome. As reactive oxygen species (ROS) produced in mitochondria can lead t ...
Pg 221- Cellular Respiration Name_________________________
... 16. Where does electron transport chain take place in the cell?________________________ 17. What is glycolysis? ______________________________________________________ ____________________________________________________________________ 18. What does glycolysis mean?__________________________________ ...
... 16. Where does electron transport chain take place in the cell?________________________ 17. What is glycolysis? ______________________________________________________ ____________________________________________________________________ 18. What does glycolysis mean?__________________________________ ...
hsf2a.zip
... promoters of hsp genes (1–3). HSF isolated from yeast, Drosophila, murine, and human cells was shown to be involved in binding to the heat shock response elements (HSE) in heat shock promoters and activating transcription (3–9). Since the isolation of hsf from Saccharomyces cerevisiae, related genes ...
... promoters of hsp genes (1–3). HSF isolated from yeast, Drosophila, murine, and human cells was shown to be involved in binding to the heat shock response elements (HSE) in heat shock promoters and activating transcription (3–9). Since the isolation of hsf from Saccharomyces cerevisiae, related genes ...
Slide 1 - McGill University
... polypeptide chain and the last two of the other. Such a model is useful to rationalize the functional complementation observed when mutant or chimeric receptors are coexpressed. b | Second is the contact model in which each polypeptide forms a receptor unit that touches the other through interaction ...
... polypeptide chain and the last two of the other. Such a model is useful to rationalize the functional complementation observed when mutant or chimeric receptors are coexpressed. b | Second is the contact model in which each polypeptide forms a receptor unit that touches the other through interaction ...
Auxin-Dependent Cell Division and Cell Elongation. 1
... These findings implicate that auxin must control cell division and cell elongation through different pathways. This conclusion is supported by auxin response mutants such as alf4 or axr6, where cell division and cell elongation are uncoupled (Celenza et al., 1995; Hobbie et al., 2000). In fact, this ...
... These findings implicate that auxin must control cell division and cell elongation through different pathways. This conclusion is supported by auxin response mutants such as alf4 or axr6, where cell division and cell elongation are uncoupled (Celenza et al., 1995; Hobbie et al., 2000). In fact, this ...
Manuscrit en préparation (soumission prévue à Int - HAL
... 5 min. Total lipids were then immediately extracted from the cell pellet according to the method of Bligh and Dyer [32]. Lipid extract was evaporated under vacuum and lipid classes were separated by silica gel thin layer chromatography with hexane/diethyl ether/acetic acid (70/30/1, v/v). Phospholip ...
... 5 min. Total lipids were then immediately extracted from the cell pellet according to the method of Bligh and Dyer [32]. Lipid extract was evaporated under vacuum and lipid classes were separated by silica gel thin layer chromatography with hexane/diethyl ether/acetic acid (70/30/1, v/v). Phospholip ...
The Effect of Osmotic Shock on Release of Bacterial Proteins and on
... called the shock fluid, is removed. This fluid contains the hydrolytic enzymes already referred to (Table I) as well as the binding proteins. It contains about 3.5 % of the cellular protein when the cells are grown under conditions that suppress the synthesis of alkaline phosphatase. T h e shocked c ...
... called the shock fluid, is removed. This fluid contains the hydrolytic enzymes already referred to (Table I) as well as the binding proteins. It contains about 3.5 % of the cellular protein when the cells are grown under conditions that suppress the synthesis of alkaline phosphatase. T h e shocked c ...
Defining the three cell lineages of the human blastocyst by single
... genes, we selected the key pluripotency-associated factors Pou5f1/ POU5F1, Sox2/SOX2 and Nanog/NANOG and followed their temporal expression dynamics (Fig. 1B). Pou5f1/POU5F1 (human cluster 49 and mouse cluster 4) shows an upregulation of expression from the 4-cell to the blastocyst stage. However, t ...
... genes, we selected the key pluripotency-associated factors Pou5f1/ POU5F1, Sox2/SOX2 and Nanog/NANOG and followed their temporal expression dynamics (Fig. 1B). Pou5f1/POU5F1 (human cluster 49 and mouse cluster 4) shows an upregulation of expression from the 4-cell to the blastocyst stage. However, t ...
The functional cobalamin (vitamin B12)–intrinsic factor receptor is a
... site (CUB domains 5-8).5,6 Although cubilin has no apparent transmembrane segment or cytoplasmic tail, several studies have shown that binding of IF-cobalamin to cubilin leads to endocytosis of the ligand and recycling of the receptor.2,3 Besides expression and function in the intestine, cubilin has ...
... site (CUB domains 5-8).5,6 Although cubilin has no apparent transmembrane segment or cytoplasmic tail, several studies have shown that binding of IF-cobalamin to cubilin leads to endocytosis of the ligand and recycling of the receptor.2,3 Besides expression and function in the intestine, cubilin has ...
Polysialic acid controls NCAM signals at cell–cell contacts to
... Fig. 3. Removal of polySia and trans-interacting NCAM enhances focal adhesion. (A,B) Detection of peripheral focal adhesions with FAK-specific antibody (red), actin staining with FITC–phalloidin (green) and nuclear counterstain with DAPI (blue) in LS cell transfectants expressing polysialylated NCAM ...
... Fig. 3. Removal of polySia and trans-interacting NCAM enhances focal adhesion. (A,B) Detection of peripheral focal adhesions with FAK-specific antibody (red), actin staining with FITC–phalloidin (green) and nuclear counterstain with DAPI (blue) in LS cell transfectants expressing polysialylated NCAM ...
A-new-precipitation-technique-provides-evidence-for-the
... probe to measure steady-state pressures in the system as described by Ranathunge et al. (2003). A syringe was filled with 100 mOsmol kg-1 CuSO4 and mounted on the pump, and the cell wall tube was perfused at a pump rate of either by 3 ¥ 10-11 or 6 ¥ 10-11 m3 s-1. Pressure in the system increased gra ...
... probe to measure steady-state pressures in the system as described by Ranathunge et al. (2003). A syringe was filled with 100 mOsmol kg-1 CuSO4 and mounted on the pump, and the cell wall tube was perfused at a pump rate of either by 3 ¥ 10-11 or 6 ¥ 10-11 m3 s-1. Pressure in the system increased gra ...
The UDPase activity of the Kluyveromyces lactis Golgi GDPase has
... with Golgi vesicles of mammalian cells. Evidence of its physiological relevance has been obtained in vivo and in vitro in Saccharomyces cerevisiae (Abeijon et al., 1993; Berninsone et al., 1994). Following identification and purification of a Golgi lumenal, membrane-bound GDPase, the gene encoding t ...
... with Golgi vesicles of mammalian cells. Evidence of its physiological relevance has been obtained in vivo and in vitro in Saccharomyces cerevisiae (Abeijon et al., 1993; Berninsone et al., 1994). Following identification and purification of a Golgi lumenal, membrane-bound GDPase, the gene encoding t ...
G1 Phase-Dependent Expression of Bcl
... PBS and reculture in the growth medium. At each time point, onethird of the cells were harvested for Western blotting assay, onethird for cell cycle analysis, and one-third were treated for 3 or 24 h with either etoposide (50 M/ml), cisplatin (20 M/ml), or staurosporin (1 M/ml). After each treatm ...
... PBS and reculture in the growth medium. At each time point, onethird of the cells were harvested for Western blotting assay, onethird for cell cycle analysis, and one-third were treated for 3 or 24 h with either etoposide (50 M/ml), cisplatin (20 M/ml), or staurosporin (1 M/ml). After each treatm ...
Synergistic interaction between C5a and NOD2 signaling
... The innate immune response is a complex process involving multiple pathogen-recognition receptors, including toll-like receptors (TLRs) and nucleotidebinding oligomerization domain (NOD)-like receptors. Complement is also a critical component of innate immunity. While complement is known to interact ...
... The innate immune response is a complex process involving multiple pathogen-recognition receptors, including toll-like receptors (TLRs) and nucleotidebinding oligomerization domain (NOD)-like receptors. Complement is also a critical component of innate immunity. While complement is known to interact ...