Application of molecular markers in plant breeding
... sacrificed to score its phenotype (e.g. male sterility in Brassica napus, final attenuation in malting barley). -alleles not expressed in the selection environment. -combining traits that might mask each other’s effects (e.g. pyramiding resistance genes). Modern plant breeding is not only based on g ...
... sacrificed to score its phenotype (e.g. male sterility in Brassica napus, final attenuation in malting barley). -alleles not expressed in the selection environment. -combining traits that might mask each other’s effects (e.g. pyramiding resistance genes). Modern plant breeding is not only based on g ...
Prediction and investigation of novel proteins in DNA double
... DSBs are primarily repaired by two independent and highly conserved pathways: homologous recombination (HR) and non-homologous end joining (NHEJ). HR requires a homologous sequence to repair DNA breaks, whereas NHEJ repair is achieved through direct ligation of the broken ends of DNA. The process of ...
... DSBs are primarily repaired by two independent and highly conserved pathways: homologous recombination (HR) and non-homologous end joining (NHEJ). HR requires a homologous sequence to repair DNA breaks, whereas NHEJ repair is achieved through direct ligation of the broken ends of DNA. The process of ...
Oxalate decarboxylase of the white-rot fungus
... enzymes since only small amounts of extracellular ODC activities have been detected, secreted either to the culture medium or to the fungal cell wall and extracellular polysaccharide layer (Dutton et al., 1994; Kathiara et al., 2000). It is assumed that the major role of ODC in fungi is to prevent t ...
... enzymes since only small amounts of extracellular ODC activities have been detected, secreted either to the culture medium or to the fungal cell wall and extracellular polysaccharide layer (Dutton et al., 1994; Kathiara et al., 2000). It is assumed that the major role of ODC in fungi is to prevent t ...
DNA METHODS FOR HLA TYPING A WORKBOOK FOR - ASHI-U
... Hematopoietic stem cells found in the bone marrow differentiate to become blood cells. These cells play important roles in fighting infection (white blood cells or lymphocytes), in transporting oxygen to the tissues (red blood cells), and in causing blood clotting (platelets). Certain types of cance ...
... Hematopoietic stem cells found in the bone marrow differentiate to become blood cells. These cells play important roles in fighting infection (white blood cells or lymphocytes), in transporting oxygen to the tissues (red blood cells), and in causing blood clotting (platelets). Certain types of cance ...
Adaptation of Drosophila to a novel laboratory environment reveals
... traits to selection. Thus, despite a large number of studies, very little is known about the underlying genetic trajectories. Studies that have pioneered the analysis of the genetic signature in experimental Drosophila populations have used allozymes and later microsatellites (Rand et al. 2010) or s ...
... traits to selection. Thus, despite a large number of studies, very little is known about the underlying genetic trajectories. Studies that have pioneered the analysis of the genetic signature in experimental Drosophila populations have used allozymes and later microsatellites (Rand et al. 2010) or s ...
Functional cooperation between the non-paralogous
... reduction rate was then calculated for each genotype. Less extensive data have also been collected for 1-, 3- and 6-day-old double mutant animals. For the analysis of the vertebral skeleton, the limits of the transformed region was sometimes equivocal because (i) the natural individual variability i ...
... reduction rate was then calculated for each genotype. Less extensive data have also been collected for 1-, 3- and 6-day-old double mutant animals. For the analysis of the vertebral skeleton, the limits of the transformed region was sometimes equivocal because (i) the natural individual variability i ...
View/print full test page
... If microarray analysis is performed, it will be done using a high resolution, single nucleotide polymorphism (SNP) platform designed to interrogate the whole genome at a resolution much higher than is possible using traditional karyotyping or fluorescence in situ hybridization (FISH) methodologies. ...
... If microarray analysis is performed, it will be done using a high resolution, single nucleotide polymorphism (SNP) platform designed to interrogate the whole genome at a resolution much higher than is possible using traditional karyotyping or fluorescence in situ hybridization (FISH) methodologies. ...
Pitx1 and Pitx2 are required for development of hindlimb buds
... in Pitx1–/– embryos (Lanctôt et al., 1999b; Szeto et al., 1999) and which is similarly decreased in double mutant embryos (Fig. 4A). Thus, HL bud size reduction affects both outgrowth and width of the bud along the AP axis. Bud outgrowth is thought to be controlled by growth factors produced by the ...
... in Pitx1–/– embryos (Lanctôt et al., 1999b; Szeto et al., 1999) and which is similarly decreased in double mutant embryos (Fig. 4A). Thus, HL bud size reduction affects both outgrowth and width of the bud along the AP axis. Bud outgrowth is thought to be controlled by growth factors produced by the ...
Characterization of lysine decarboxylase
... profiles. Their lysine-decarboxylase phenotype was recovered by introduction of the cadBA locus from an lysine-decarboxylase-positive strain. Although the cad loci of the lysine-decarboxylase-negative strains seemed to be intact without any insertion sequences, cadC, a positive regulator of cadBA, h ...
... profiles. Their lysine-decarboxylase phenotype was recovered by introduction of the cadBA locus from an lysine-decarboxylase-positive strain. Although the cad loci of the lysine-decarboxylase-negative strains seemed to be intact without any insertion sequences, cadC, a positive regulator of cadBA, h ...
Intervention, integration and translation in obesity research: Genetic
... Arguably an even more important finding was that of the Lep gene and its product leptin, from the Greek for ‘thin’, The recessive gene mutation (Lepob – ob for obesity) had been linked to obesity and type 2 diabetes since the early 1950s, as was the mutant db of the leptin receptor gene (Lepr db ) [ ...
... Arguably an even more important finding was that of the Lep gene and its product leptin, from the Greek for ‘thin’, The recessive gene mutation (Lepob – ob for obesity) had been linked to obesity and type 2 diabetes since the early 1950s, as was the mutant db of the leptin receptor gene (Lepr db ) [ ...
PDF version - EpiGeneSys
... agarose gel (comment 4) electrophoresis. 5µl of each reconstitution mixture is mixed with 5µl running buffer (0.2X TBE) and 2µl gel loading buffer (20% glycerol, 20mM Tris pH 7.4, 1mM EDTA, 0.1% bromophenol blue) and loaded onto a 0.8% agarose gel (normally 10x10cm) in 0.2X TBE buffer. The gel is ru ...
... agarose gel (comment 4) electrophoresis. 5µl of each reconstitution mixture is mixed with 5µl running buffer (0.2X TBE) and 2µl gel loading buffer (20% glycerol, 20mM Tris pH 7.4, 1mM EDTA, 0.1% bromophenol blue) and loaded onto a 0.8% agarose gel (normally 10x10cm) in 0.2X TBE buffer. The gel is ru ...
Using Drosophila to Understand the Genetics of Circadian Rhythms
... when a molecular assay for circadian entrainment became available. The TIM protein is degraded in response to light, and it mediates resetting of the molecular clock and of the ensuing behavioral rhythm.26,27 Flies lacking eyes or some of the components of the visual system are able to degrade TIM a ...
... when a molecular assay for circadian entrainment became available. The TIM protein is degraded in response to light, and it mediates resetting of the molecular clock and of the ensuing behavioral rhythm.26,27 Flies lacking eyes or some of the components of the visual system are able to degrade TIM a ...
The Genetic Basis of Lateralization
... error. In tests of the model, right- and left-handers were moved between genotypes to match observed incidences. The model cannot fail, therefore, unless observed incidences are very different from the supposed true incidence. With regard to cerebral dominance, D is expected to give left hemisphere ...
... error. In tests of the model, right- and left-handers were moved between genotypes to match observed incidences. The model cannot fail, therefore, unless observed incidences are very different from the supposed true incidence. With regard to cerebral dominance, D is expected to give left hemisphere ...
An Introduction to RNA Interference (RNAi)
... Another major advantage that comes from harnessing an endogenous biological pathway is the ability to translate knowledge on normal function into the development of even safer and more efficacious therapeutics. Indeed, due to their central importance in many cell biological processes, an unpreceden ...
... Another major advantage that comes from harnessing an endogenous biological pathway is the ability to translate knowledge on normal function into the development of even safer and more efficacious therapeutics. Indeed, due to their central importance in many cell biological processes, an unpreceden ...
Read the Nobel Lecture
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
... First we needed an assay – a way to detect if telomere elongation was happening. The first assay we tried explored whether a piece of DNA that included a telomere would incorporate DNA precursors more readily than a piece of DNA containing non-telomeric sequences. The idea was that if there was an ...
Investigation of Genes That May Be Required for the
... individual experiences, many of them small, that together make a significant contribution to when one dies (Vaupel et al.,1998; Finch and Kirkwood, 2000; Herndon et al., 2002; Kirkwood and Finch, 2002; Rea et al., 2005). In individual wildtype C. elegans, levels of certain biomarkers, such as sod-3, ...
... individual experiences, many of them small, that together make a significant contribution to when one dies (Vaupel et al.,1998; Finch and Kirkwood, 2000; Herndon et al., 2002; Kirkwood and Finch, 2002; Rea et al., 2005). In individual wildtype C. elegans, levels of certain biomarkers, such as sod-3, ...
The genetics of diabetes mellitus
... transmission have revealed that the class I allele does not predispose to disease when paternally inherited, suggestive of polymorphic imprinting, but this paternal effect is observed only when the father’s untransmitted allele is a class III19,20. Locus on chromosome 15q26: Subjects with a suscepti ...
... transmission have revealed that the class I allele does not predispose to disease when paternally inherited, suggestive of polymorphic imprinting, but this paternal effect is observed only when the father’s untransmitted allele is a class III19,20. Locus on chromosome 15q26: Subjects with a suscepti ...
Structural and molecular differentiation of sex
... Y (or W) chromosome. Accumulation of self-replicating transposable elements is though to be enabled for several reasons. First, once the genetic activity of the Y (or W) is reduced there is a low probability of insertion into a transcriptionally active region and thereby its inactivation, which coul ...
... Y (or W) chromosome. Accumulation of self-replicating transposable elements is though to be enabled for several reasons. First, once the genetic activity of the Y (or W) is reduced there is a low probability of insertion into a transcriptionally active region and thereby its inactivation, which coul ...
Loss of heterozygosity at D8S262: an early genetic event of
... that surveillance of the at-risk cirrhotic population could aid earlier detection of the disease and decrease the cancer-related mortality rate, but we are limited by the lack of sensitive biomarkers and reliable histopathological features of precancerous lesions. ...
... that surveillance of the at-risk cirrhotic population could aid earlier detection of the disease and decrease the cancer-related mortality rate, but we are limited by the lack of sensitive biomarkers and reliable histopathological features of precancerous lesions. ...
Morphology of nuclear transcription | SpringerLink
... or from separate CTs. In the latter case, they can form long-range loops as often observed in coregulated loci during cell differentiation processes (Park et al. 2014). This spatial organization is understood to be pivotal for transcription linking chromatin architecture to coordinated gene expressi ...
... or from separate CTs. In the latter case, they can form long-range loops as often observed in coregulated loci during cell differentiation processes (Park et al. 2014). This spatial organization is understood to be pivotal for transcription linking chromatin architecture to coordinated gene expressi ...
Epigenetic Interactions among Three dTph1 Transposons in Two
... of progeny with an an3mut phenotype similar to the parental line and z50% with an An31 revertant phenotype (data not shown). In progenies obtained by selfing R134/R134 or S205/S205 plants, we found at a low frequency (1 to 5%) plants with a very low spotting frequency (weakly an3 mut) that were ofte ...
... of progeny with an an3mut phenotype similar to the parental line and z50% with an An31 revertant phenotype (data not shown). In progenies obtained by selfing R134/R134 or S205/S205 plants, we found at a low frequency (1 to 5%) plants with a very low spotting frequency (weakly an3 mut) that were ofte ...
A Novel PCR Detection Method for Major Fish Pathogenic Bacteria
... as the selective medium method(Alsinaet al., 1994), API 20E system (Grisezet al., 1991), fluorescent antibody technique (Miyamoto and Eguchi, 1997), pulsed-field gel electrophoresis analysis (Skov et al., 1995), and DNA hybridization (Martinez-Picadoet al., 1996). However, these methods require cons ...
... as the selective medium method(Alsinaet al., 1994), API 20E system (Grisezet al., 1991), fluorescent antibody technique (Miyamoto and Eguchi, 1997), pulsed-field gel electrophoresis analysis (Skov et al., 1995), and DNA hybridization (Martinez-Picadoet al., 1996). However, these methods require cons ...
CD40/CD40L Dyad in the Inflammatory and Immune Responses in
... terminal aa of human CD40 are completely conserved in the mouse sequence. In addition, 22 extracellular cysteine residues are conserved, suggesting that both mouse and human CD40 fold into a very similar configuration. Because of the similarity in the extracellular segment, and in particular, its 22 ...
... terminal aa of human CD40 are completely conserved in the mouse sequence. In addition, 22 extracellular cysteine residues are conserved, suggesting that both mouse and human CD40 fold into a very similar configuration. Because of the similarity in the extracellular segment, and in particular, its 22 ...
Fig 16.12a - McGraw Hill Higher Education
... Enhanceosome – multimeric complex of proteins and other small molecules that associate with an enhancer • Enhancers can be bound by activators and repressors with varying affinities • Different sets of cofactors and corepressors compete for binding to activators and repressors Copyright © The McGraw ...
... Enhanceosome – multimeric complex of proteins and other small molecules that associate with an enhancer • Enhancers can be bound by activators and repressors with varying affinities • Different sets of cofactors and corepressors compete for binding to activators and repressors Copyright © The McGraw ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse