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... Lethal hybrid rescue (Lhr) on chromosome II, interact to cause this lethality. Loss-of-function mutations in either gene suppress lethality, but several pieces of evidence suggest that additional factors are required for hybrid lethality. Here we screen the D. melanogaster autosomal genome by using ...
... Lethal hybrid rescue (Lhr) on chromosome II, interact to cause this lethality. Loss-of-function mutations in either gene suppress lethality, but several pieces of evidence suggest that additional factors are required for hybrid lethality. Here we screen the D. melanogaster autosomal genome by using ...
Codon usage bias from tRNA`s point of view
... species. Surprisingly, despite the wide G+C variation of bacterial genomes these anticodons are the same in most genomes. This suggests an optimization of the translation machinery to use a small subset of optimal codons and anticodons in fast-growing bacteria and in highly expressed genes. As a res ...
... species. Surprisingly, despite the wide G+C variation of bacterial genomes these anticodons are the same in most genomes. This suggests an optimization of the translation machinery to use a small subset of optimal codons and anticodons in fast-growing bacteria and in highly expressed genes. As a res ...
Giant viruses, giant chimeras: The multiple evolutionary histories of
... and Eukaryotes [12]. Such an unexpected result had the potential to revolutionise our conceptions on the diversity and evolution of life, up to now based on the tripartite scheme of the three domains [13]. Raoult et al. further suggested that the NCLDV family may have played a role in the origin of ...
... and Eukaryotes [12]. Such an unexpected result had the potential to revolutionise our conceptions on the diversity and evolution of life, up to now based on the tripartite scheme of the three domains [13]. Raoult et al. further suggested that the NCLDV family may have played a role in the origin of ...
Construction of a genetic linkage map of Thlaspi
... Codominant CAPS/Indel markers PCR-based codominant markers were developed for the accessions LE and LC. The markers were based on Expressed Sequence Tags (ESTs), from an EST library of T. caerulescens accession LC (D. Rigola & M. G. M. Aarts, unpublished results), for which homologous genes were fou ...
... Codominant CAPS/Indel markers PCR-based codominant markers were developed for the accessions LE and LC. The markers were based on Expressed Sequence Tags (ESTs), from an EST library of T. caerulescens accession LC (D. Rigola & M. G. M. Aarts, unpublished results), for which homologous genes were fou ...
Phenotype Curation Tool and Ontologies at dictyBase
... • Developed in collaboration with NCBO • Similar in functionality to Phenote • Web-based tool that can be accessed from any computer ...
... • Developed in collaboration with NCBO • Similar in functionality to Phenote • Web-based tool that can be accessed from any computer ...
Development and Optimization of a DNA extraction
... discarded. For tests with BB2 and BB4, the lysis was performed with the commercial kits by following the protocol until the filtration step and, then, the filtered lysate was added to the corresponding binding buffer in a ratio 1:1. This protocol was repeated as needed in order to have the same samp ...
... discarded. For tests with BB2 and BB4, the lysis was performed with the commercial kits by following the protocol until the filtration step and, then, the filtered lysate was added to the corresponding binding buffer in a ratio 1:1. This protocol was repeated as needed in order to have the same samp ...
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... Fraser, 1996), the chick (Hensen’s node)(Storey et al., 1992; Waddington, 1933) and the mouse (the node)(Beddington, 1994; Tam and Steiner, 1999), suggesting that the organizer is a conserved element in vertebrate embryonic patterning. As well as inducing correct D-V patterning in the developing mes ...
... Fraser, 1996), the chick (Hensen’s node)(Storey et al., 1992; Waddington, 1933) and the mouse (the node)(Beddington, 1994; Tam and Steiner, 1999), suggesting that the organizer is a conserved element in vertebrate embryonic patterning. As well as inducing correct D-V patterning in the developing mes ...
of Lactobacillus pentosus
... 1992). Although, more than 150 Lactobadillus genes from different strains have been cloned and sequenced, specific knowledge about gene expression and control of gene expression in Lactobacillus is stili scarce. The genetic analysis of lactic acid bacteria has mostly been tocused on lactococci strai ...
... 1992). Although, more than 150 Lactobadillus genes from different strains have been cloned and sequenced, specific knowledge about gene expression and control of gene expression in Lactobacillus is stili scarce. The genetic analysis of lactic acid bacteria has mostly been tocused on lactococci strai ...
Tomé, S., Manley, K., Simard, J.P., Clark, G.W., Slean, M.M., Swami
... proteins, like PMS2 are partially required for instability [28]. The MutSb complex may act on CAG repeats during errors at replication forks or during transcription, as both processes can enhance instability in a MMR-dependant manner [51,52]. Instability in non-proliferating tissues may arise when a ...
... proteins, like PMS2 are partially required for instability [28]. The MutSb complex may act on CAG repeats during errors at replication forks or during transcription, as both processes can enhance instability in a MMR-dependant manner [51,52]. Instability in non-proliferating tissues may arise when a ...
Klf4 Organizes Long-Range Chromosomal
... sequencing (4C-seq) and fluorescence in situ hybridization (FISH), we identified chromosomal regions that colocalize frequently with the Oct4 locus in PSCs. These PSC-specific long-range interactions are established prior to transcriptional activation of endogenous Oct4 during reprogramming to induc ...
... sequencing (4C-seq) and fluorescence in situ hybridization (FISH), we identified chromosomal regions that colocalize frequently with the Oct4 locus in PSCs. These PSC-specific long-range interactions are established prior to transcriptional activation of endogenous Oct4 during reprogramming to induc ...
handedness - UNIT NAME
... factors contributed twice the influence to left and right cerebral hemispheric volumes in right-handed twin pairs, suggesting a large decrement in genetic control of cerebral volumes in the nonright-handed twin pairs. This loss of genetic determination of left and right cerebral hemispheres in the n ...
... factors contributed twice the influence to left and right cerebral hemispheric volumes in right-handed twin pairs, suggesting a large decrement in genetic control of cerebral volumes in the nonright-handed twin pairs. This loss of genetic determination of left and right cerebral hemispheres in the n ...
AmiGO
... searches the sequences from the GO protein sequence database, which comprises protein sequences of genes and gene products that have been annotated to a GO term and submitted to the GO Consortium. ...
... searches the sequences from the GO protein sequence database, which comprises protein sequences of genes and gene products that have been annotated to a GO term and submitted to the GO Consortium. ...
A Novel Splicing Mutation of KIT Results in Piebaldism and Auburn
... matched control samples by direct sequencing. The mutation screening of MC1R gene in all available family individuals (II:3, II:4, III:1, and III:2, Figure 1(a)) detected 4 heterozygous variants of MC1R, including c.274G>A, c.359T>C, c.488 G>A, and c.942 A>G and identified neither heterogeneous nor ...
... matched control samples by direct sequencing. The mutation screening of MC1R gene in all available family individuals (II:3, II:4, III:1, and III:2, Figure 1(a)) detected 4 heterozygous variants of MC1R, including c.274G>A, c.359T>C, c.488 G>A, and c.942 A>G and identified neither heterogeneous nor ...
Genetic Characterisation of Human ABO Blood
... meaningful nomenclature for different blood groups as shown in Table 2 (Daniels et al., 2003; 2004; Storry & Olsson, 2004). Every valid blood group antigen is given a six digit identification number, according to the following categorization: - Blood group systems; one or more antigens governed eith ...
... meaningful nomenclature for different blood groups as shown in Table 2 (Daniels et al., 2003; 2004; Storry & Olsson, 2004). Every valid blood group antigen is given a six digit identification number, according to the following categorization: - Blood group systems; one or more antigens governed eith ...
VCS: Tool for Visualizing Copy Number Variation and Single Nucleotide Polymorphism
... counts and median size of gain (insertion), loss (deletion), and complex (insertion and deletion) (Figure 1B). Default of a criteria set up ±0.3 which is widely used in biology research. And you can show distribution of visualized log2 ratio and/or ±values. Variation distribution visualization per b ...
... counts and median size of gain (insertion), loss (deletion), and complex (insertion and deletion) (Figure 1B). Default of a criteria set up ±0.3 which is widely used in biology research. And you can show distribution of visualized log2 ratio and/or ±values. Variation distribution visualization per b ...
The Wnt code: cnidarians signal the way
... where it acts in organizer formation and regeneration. Wnt signalling is also essential for cnidarian embryogenesis. In the sea anemone Nematostella vectensis 11 of the 12 known wnt gene subfamilies were identified. Different wnt genes exhibit serial and overlapping expression domains along the oral– ...
... where it acts in organizer formation and regeneration. Wnt signalling is also essential for cnidarian embryogenesis. In the sea anemone Nematostella vectensis 11 of the 12 known wnt gene subfamilies were identified. Different wnt genes exhibit serial and overlapping expression domains along the oral– ...
Incorporation of reporter molecule
... The modi®cation of nucleic acids using nucleotides linked to detectable reporter or functional groups is an important experimental tool in modern molecular biology. This enhances DNA or RNA detection as well as expanding the catalytic repertoire of nucleic acids. Here we present the evaluation of a ...
... The modi®cation of nucleic acids using nucleotides linked to detectable reporter or functional groups is an important experimental tool in modern molecular biology. This enhances DNA or RNA detection as well as expanding the catalytic repertoire of nucleic acids. Here we present the evaluation of a ...
Mapping the Horns (Ho) Locus in Sheep: A Further Locus
... horns in bovid species show a number of interesting features. The expression of horns in some sheep strains has pleiotropic effects on wool fibers resulting in a highly medullated hairlike fleece (Dry 1955). Alleles at the same locus control sex-limited expression of horns or development of horns in ...
... horns in bovid species show a number of interesting features. The expression of horns in some sheep strains has pleiotropic effects on wool fibers resulting in a highly medullated hairlike fleece (Dry 1955). Alleles at the same locus control sex-limited expression of horns or development of horns in ...
A Cladistic Analysis of Phenotype Associations with
... taining genes with known biochemical functions. By simultaneously studying traits that are related to the genes’ known biochemical or physiological functions, it is possible to assign phenotypic effects to specific alleles or haplotypes at or near the genes of knownfunction. This measured genotype a ...
... taining genes with known biochemical functions. By simultaneously studying traits that are related to the genes’ known biochemical or physiological functions, it is possible to assign phenotypic effects to specific alleles or haplotypes at or near the genes of knownfunction. This measured genotype a ...
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... (p<0.001). These results are harmonious with (Khalil et al., 2014).This polymorphism is in an intron, +299 (G>A), which generally has not been considered to have regulatory functions. However, it has been shown that SNPs in the non-coding region, such as the 3 -untranslated gene region, can affect g ...
... (p<0.001). These results are harmonious with (Khalil et al., 2014).This polymorphism is in an intron, +299 (G>A), which generally has not been considered to have regulatory functions. However, it has been shown that SNPs in the non-coding region, such as the 3 -untranslated gene region, can affect g ...
Endogenous Cerberus activity is required for anterior head
... conclude that reducing Cerberus activity in the ADE was not sufficient to impair head formation in the Xenopus embryo. Gain of BMP, Nodal or Wnt function in the ADE perturbs head formation Cerberus protein can bind to and antagonize BMP4, Xnr1 and Xwnt8 molecules (Piccolo et al., 1999). We reasoned ...
... conclude that reducing Cerberus activity in the ADE was not sufficient to impair head formation in the Xenopus embryo. Gain of BMP, Nodal or Wnt function in the ADE perturbs head formation Cerberus protein can bind to and antagonize BMP4, Xnr1 and Xwnt8 molecules (Piccolo et al., 1999). We reasoned ...
Comparative Analysis Using DNA Microarrays: Sensitivity
... sensitivity of gene arrays is not nearly so simple as specificity With same-vs.-same, we had a large set of equivalently expressed genes whose SLRTRUE was, by definition, equal to zero But what to do for differentially expressed genes? ...
... sensitivity of gene arrays is not nearly so simple as specificity With same-vs.-same, we had a large set of equivalently expressed genes whose SLRTRUE was, by definition, equal to zero But what to do for differentially expressed genes? ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse