Type I Factor XI11 Deficiency Is Caused By a
... digested with restriction enzymes to generate the proper ends for ligation into sequencing vectors. The digested samples were applied to a 0.8% agarose gel, electroeluted, and then subcloned into M13mp18 or M13mp19 (GIBCO-BRL, Gathersburg, MD or Toyobo, Tokyo, Japan) with restriction sites to obtain ...
... digested with restriction enzymes to generate the proper ends for ligation into sequencing vectors. The digested samples were applied to a 0.8% agarose gel, electroeluted, and then subcloned into M13mp18 or M13mp19 (GIBCO-BRL, Gathersburg, MD or Toyobo, Tokyo, Japan) with restriction sites to obtain ...
Two concepts of natural selection and their explanatory powers
... conception of selection needs to be modified significantly. If, however, the replicationinteraction model, or at least a revised version thereof, no longer faces these objections, then we have good reason to revive it, as it may enjoy some significant explanatory advantages over the heritable variat ...
... conception of selection needs to be modified significantly. If, however, the replicationinteraction model, or at least a revised version thereof, no longer faces these objections, then we have good reason to revive it, as it may enjoy some significant explanatory advantages over the heritable variat ...
1 FRQ-interacting RNA Helicase (FRH) Mediates Negative and
... SNP6 and SNP72 and were scored among those recombinant progeny. Using these additional markers, Mut10 was mapped in a 20kb region; the two ‘aberrant’ progeny also showed recombination with all newly identified markers, supporting the idea of a chromosomal translocation tightly associated with arrhyt ...
... SNP6 and SNP72 and were scored among those recombinant progeny. Using these additional markers, Mut10 was mapped in a 20kb region; the two ‘aberrant’ progeny also showed recombination with all newly identified markers, supporting the idea of a chromosomal translocation tightly associated with arrhyt ...
In this work, the quantitative assessment of cell lysis was performed
... induction (data not shown). Lysis occurred in exponential and early stationary phases, but never in late stationary phase. Lysis behavior in high copy plasmid had a high variability between different experiments carried out in separate days, in fact lysis entity in exponential phase and early statio ...
... induction (data not shown). Lysis occurred in exponential and early stationary phases, but never in late stationary phase. Lysis behavior in high copy plasmid had a high variability between different experiments carried out in separate days, in fact lysis entity in exponential phase and early statio ...
Molecular and Genetic Characterization of a
... about the regulatory genes specifically associated with ripening. Several single-gene mutations resulting in the reduction or almost complete elimination of ripening are known in tomato (Lycopersicon esculentum) fruit (Grierson, 1986). These mutant loci include rin (ripening-inhibitor; Robinson and ...
... about the regulatory genes specifically associated with ripening. Several single-gene mutations resulting in the reduction or almost complete elimination of ripening are known in tomato (Lycopersicon esculentum) fruit (Grierson, 1986). These mutant loci include rin (ripening-inhibitor; Robinson and ...
SALSA MLPA probemix P018-F1 SHOX - MRC
... within the PAR1 region (pseudoautosomal region 1) which covers the 3000 kb of the X and Y chromosomes next to the p-telomere. This P018-F1 SHOX probemix contains probes for each exon of the human SHOX gene, as well as a probe just before the SHOX promoter region. In addition, several probes are pres ...
... within the PAR1 region (pseudoautosomal region 1) which covers the 3000 kb of the X and Y chromosomes next to the p-telomere. This P018-F1 SHOX probemix contains probes for each exon of the human SHOX gene, as well as a probe just before the SHOX promoter region. In addition, several probes are pres ...
Standard PDF - Wiley Online Library
... mercial rat colonies, and found four distinct karyotype forms. Further analysis raised the number of distinct karyotype forms in rats to eight [15]. Karyotype forms of P. carinii f.sp. carinii have been shown to be stable for at least a year [8]. A particular karyotype form can occur in multiple col ...
... mercial rat colonies, and found four distinct karyotype forms. Further analysis raised the number of distinct karyotype forms in rats to eight [15]. Karyotype forms of P. carinii f.sp. carinii have been shown to be stable for at least a year [8]. A particular karyotype form can occur in multiple col ...
Effect of population size, selection intensity, linkage and non
... rate, such selection operated, in complex epistatic systems, to modify the relation of the genotype to the phenotype into a sigmoid function. ...
... rate, such selection operated, in complex epistatic systems, to modify the relation of the genotype to the phenotype into a sigmoid function. ...
Regulation of limb bud initiation and limbtype
... bud will appear and, thus, their identity (Cohn et al., 1997; Cohn and Tickle, 1999) (Fig. 1A). For example, Hoxc4-5 is expressed mostly in the mesenchyme of the presumptive forelimb area (Savard et al., 1988; Tabin, 1989; Molven et al., 1990; Burke et al., 1995), Hoxc9, Hoxc10, Hoxc11 are, predomin ...
... bud will appear and, thus, their identity (Cohn et al., 1997; Cohn and Tickle, 1999) (Fig. 1A). For example, Hoxc4-5 is expressed mostly in the mesenchyme of the presumptive forelimb area (Savard et al., 1988; Tabin, 1989; Molven et al., 1990; Burke et al., 1995), Hoxc9, Hoxc10, Hoxc11 are, predomin ...
Genome-Wide Association Mapping Reveals Novel QTL for
... made it feasible to genotype a large collection of germplasm lines with thousands of SNP markers. As a result, the GWAS approach, based on the principle of linkage disequilibrium (LD), has been effectively used to exploit existing allelic diversity for traits of agronomic importance. Compared with b ...
... made it feasible to genotype a large collection of germplasm lines with thousands of SNP markers. As a result, the GWAS approach, based on the principle of linkage disequilibrium (LD), has been effectively used to exploit existing allelic diversity for traits of agronomic importance. Compared with b ...
Chapter 1
... ► Is difficult to classify alpha thalassemias due to wide variety of possible genetic combinations. ► Absence of alpha chains will result in increase of gamma chains during fetal life and excess beta chains later in life; Causes molecules like Bart's Hemoglobin (γ4) or Hemoglobin H (β4), which are ...
... ► Is difficult to classify alpha thalassemias due to wide variety of possible genetic combinations. ► Absence of alpha chains will result in increase of gamma chains during fetal life and excess beta chains later in life; Causes molecules like Bart's Hemoglobin (γ4) or Hemoglobin H (β4), which are ...
WEREWOLF, a Regulator of Root Hair Pattern
... addition, the heterozygous lines obtained from the cross between Col and wer-1 showed similar flowering time with Col, confirming that wer is a recessive lateflowering mutant (Supplemental Fig. S1). The flowering time in Arabidopsis is regulated by four major pathways: photoperiod, vernalization, au ...
... addition, the heterozygous lines obtained from the cross between Col and wer-1 showed similar flowering time with Col, confirming that wer is a recessive lateflowering mutant (Supplemental Fig. S1). The flowering time in Arabidopsis is regulated by four major pathways: photoperiod, vernalization, au ...
The red head and neck of Boer goats may be controlled by the
... head and neck color of Boer goats is brown or red except for a white band in the region from the middle of the forehead to the extremity of the nose, and their body color is white. Li et al. [5] confirmed that the red head and neck of Boer goats are controlled by one recessive gene on an autosome th ...
... head and neck color of Boer goats is brown or red except for a white band in the region from the middle of the forehead to the extremity of the nose, and their body color is white. Li et al. [5] confirmed that the red head and neck of Boer goats are controlled by one recessive gene on an autosome th ...
Charge Transport in DNA - Insights from
... The state of knowledge of DNA stretching in 2000 was summarized in two reviews. [24, 25] At that time, a dispute on the nature of the overstretched state of dsDNA started, and it is in fact still going on. In contrast to the proposal of S-DNA, the overstretching profile of DNA was attributed to force ...
... The state of knowledge of DNA stretching in 2000 was summarized in two reviews. [24, 25] At that time, a dispute on the nature of the overstretched state of dsDNA started, and it is in fact still going on. In contrast to the proposal of S-DNA, the overstretching profile of DNA was attributed to force ...
A family of thermostable fungal cellulases created by structure-guided recombination
... when the blocks are swapped among different parents. Meyer et al. (4) found that the probability that a -lactamase chimera was folded and active was inversely related to the value of E for that sequence. The RASPP (Recombination as Shortest Path Problem) algorithm (6) was used to identify the block ...
... when the blocks are swapped among different parents. Meyer et al. (4) found that the probability that a -lactamase chimera was folded and active was inversely related to the value of E for that sequence. The RASPP (Recombination as Shortest Path Problem) algorithm (6) was used to identify the block ...
Genetic analysis of seed and flower colour in flax (Linum
... with logarithm of odds (LOD) above 39 and more than 84 % of the trait expressed by the major locus in both trials. As there were no recombinants (off types) for flower colour in F8:9 plants i.e brown-seeded lines produced blue flowers and yellow-seeded lines produced white flowers, the ii ...
... with logarithm of odds (LOD) above 39 and more than 84 % of the trait expressed by the major locus in both trials. As there were no recombinants (off types) for flower colour in F8:9 plants i.e brown-seeded lines produced blue flowers and yellow-seeded lines produced white flowers, the ii ...
Randa Elfadil Ibrahim abdalla
... Our results indicate that total level of serum IgE and IL4 in patients were considerably higher than controls. Serum electrophoresis showed that the distribution of Hp phenotypes of Hp1-1, Hp2-1 and Hp2-2 among asthmatic patients were17.5%, 50.8 % and 31.7%, respectively. Distributions of different ...
... Our results indicate that total level of serum IgE and IL4 in patients were considerably higher than controls. Serum electrophoresis showed that the distribution of Hp phenotypes of Hp1-1, Hp2-1 and Hp2-2 among asthmatic patients were17.5%, 50.8 % and 31.7%, respectively. Distributions of different ...
Two supernumerary marker chromosomes
... Interestingly, in one case reported in the literature two similar familial marker chromosomes lead to very different clinical abnormalities (see Table 2, case 6). These phenotypic differences in a mother and child can only be explained by the higher frequency of one mosaic cell line with an addition ...
... Interestingly, in one case reported in the literature two similar familial marker chromosomes lead to very different clinical abnormalities (see Table 2, case 6). These phenotypic differences in a mother and child can only be explained by the higher frequency of one mosaic cell line with an addition ...
Potential alteration of U37K paleothermometer due to selective
... Aerobic cultures were incubated at 20 1C in 250-mL Erlenmeyer flasks containing 50 mL of Eh medium and sterilized freeze-dried cells of E. huxleyi strain CS-57 (12 mg per flask) or purified methyl alkenones (1 mg per flask) were used as a substrate for alkenone degradation. The flasks were agitated ...
... Aerobic cultures were incubated at 20 1C in 250-mL Erlenmeyer flasks containing 50 mL of Eh medium and sterilized freeze-dried cells of E. huxleyi strain CS-57 (12 mg per flask) or purified methyl alkenones (1 mg per flask) were used as a substrate for alkenone degradation. The flasks were agitated ...
Mapping quantitative trait loci with dominant and missing markers in
... individual. A consequence of using this incomplete information for mapping quantitative trait loci (QTL) is that the inference of the genotype of a putative QTL flanked by a marker with dominant phenotype will depend on the genotype or phenotype of the next marker. This dependence can be extended fu ...
... individual. A consequence of using this incomplete information for mapping quantitative trait loci (QTL) is that the inference of the genotype of a putative QTL flanked by a marker with dominant phenotype will depend on the genotype or phenotype of the next marker. This dependence can be extended fu ...
Human pigmentation variation: Evolution, genetic basis, and
... genes involved in normal pigmentation variation in human populations. At least six genes have been identified using genotype/phenotype association studies and/or direct functional assays, and there is evidence indicating that several additional genes may be playing a role in skin, hair, and iris pig ...
... genes involved in normal pigmentation variation in human populations. At least six genes have been identified using genotype/phenotype association studies and/or direct functional assays, and there is evidence indicating that several additional genes may be playing a role in skin, hair, and iris pig ...
Human pigmentation variation: Evolution, genetic basis, and
... genes involved in normal pigmentation variation in human populations. At least six genes have been identified using genotype/phenotype association studies and/or direct functional assays, and there is evidence indicating that several additional genes may be playing a role in skin, hair, and iris pig ...
... genes involved in normal pigmentation variation in human populations. At least six genes have been identified using genotype/phenotype association studies and/or direct functional assays, and there is evidence indicating that several additional genes may be playing a role in skin, hair, and iris pig ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse