Supplement_2_-_PLoS_
... The cloned cDNA encodes a protein of 562 amino acids with a domain organization similar to that of other MT-MMPs, including a prodomain with a cysteine switch, a catalytic domain with the zinc-binding site, a hemopexin-like domain, and a COOH-terminal extension rich in hydrophobic residues. ...
... The cloned cDNA encodes a protein of 562 amino acids with a domain organization similar to that of other MT-MMPs, including a prodomain with a cysteine switch, a catalytic domain with the zinc-binding site, a hemopexin-like domain, and a COOH-terminal extension rich in hydrophobic residues. ...
Identification of Tissue Specific Transcription Factors Using
... probes covering 810 genes encoding transcription factors were taken for the analysis. For every probe we made a plot showing its expression profile for 78 cell/tissue types. We classified all the profiles into three major groups. The first and largest group contains genes that show ubiquitous expres ...
... probes covering 810 genes encoding transcription factors were taken for the analysis. For every probe we made a plot showing its expression profile for 78 cell/tissue types. We classified all the profiles into three major groups. The first and largest group contains genes that show ubiquitous expres ...
Regulation of cellular homoeostasis by reversible lysine acetylation
... residue may inactivate it). Finally, there may be cross-talk between different types of lysine PTM (ubiquitination, SUMOylation, methylation etc.), all of which occur at the same lysine residue ε-amino group. As such, the addition of another type of lysine PTM may block the ability of a protein to b ...
... residue may inactivate it). Finally, there may be cross-talk between different types of lysine PTM (ubiquitination, SUMOylation, methylation etc.), all of which occur at the same lysine residue ε-amino group. As such, the addition of another type of lysine PTM may block the ability of a protein to b ...
Document
... negatively charged. The covalent attachment of acetyl groups decreases the amount of positive charge on the histone proteins and thereby may decrease the binding of the DNA. In addition, histone acetylation may attract proteins to the region that loosen chromatin compaction. C20. The binding of the ...
... negatively charged. The covalent attachment of acetyl groups decreases the amount of positive charge on the histone proteins and thereby may decrease the binding of the DNA. In addition, histone acetylation may attract proteins to the region that loosen chromatin compaction. C20. The binding of the ...
C1. The common points of control are as follows: 1. DNA
... negatively charged. The covalent attachment of acetyl groups decreases the amount of positive charge on the histone proteins and thereby may decrease the binding of the DNA. In addition, histone acetylation may attract proteins to the region that loosen chromatin compaction. C20. The binding of the ...
... negatively charged. The covalent attachment of acetyl groups decreases the amount of positive charge on the histone proteins and thereby may decrease the binding of the DNA. In addition, histone acetylation may attract proteins to the region that loosen chromatin compaction. C20. The binding of the ...
Regulation of Gene Expression
... collection of controlling sites adjacent to polycistronic proteincoding sequences. ...
... collection of controlling sites adjacent to polycistronic proteincoding sequences. ...
power point presentation
... The data collected through different approach can be used as reference to each other for possible final confidential result. ...
... The data collected through different approach can be used as reference to each other for possible final confidential result. ...
problem set
... a repression domain. Some TFs also contain a ligand binding domain that regulates activity. Domains typically are joined together in a single polypeptide by flexible linker sequences that serve as hinges and allow conformational changes needed for activation/repression. Some examples of transcriptio ...
... a repression domain. Some TFs also contain a ligand binding domain that regulates activity. Domains typically are joined together in a single polypeptide by flexible linker sequences that serve as hinges and allow conformational changes needed for activation/repression. Some examples of transcriptio ...
L` `epigenetica` si riferisce a tutti i cambiamenti dell`espressione
... -Histone PTMs (NC RNAs) ...
... -Histone PTMs (NC RNAs) ...
Chapter 7A
... The control of gene expression by transcription activation and repression has been studied extensively in bacteria. As an example, the E. coli lac operon, which encodes 3 genes (lacZYA) involved in lactose metabolism, uses both mechanisms of control (Fig. 7.3). A specific repressor protein (the lac ...
... The control of gene expression by transcription activation and repression has been studied extensively in bacteria. As an example, the E. coli lac operon, which encodes 3 genes (lacZYA) involved in lactose metabolism, uses both mechanisms of control (Fig. 7.3). A specific repressor protein (the lac ...
Eukaryotic Gene Regulation
... o Each cell of a multicellular eukaryote expresses only a small fraction of its genes. • Development o Some gene expression must be controlled on a long-term basis for cellular differentiation & specialization during development. • Responding to organism’s needs o Cells of multicellular organisms mu ...
... o Each cell of a multicellular eukaryote expresses only a small fraction of its genes. • Development o Some gene expression must be controlled on a long-term basis for cellular differentiation & specialization during development. • Responding to organism’s needs o Cells of multicellular organisms mu ...
401Lecture5sp2013post
... Each probe specific for sequences separated by known distances in linear Fig. 6-35 Lodish et al. 2013 DNA What result would you expect if DNA exists in loops? Would you expect loops to be present at all stages of cell cycle? ...
... Each probe specific for sequences separated by known distances in linear Fig. 6-35 Lodish et al. 2013 DNA What result would you expect if DNA exists in loops? Would you expect loops to be present at all stages of cell cycle? ...
A Tale of Three Inferences
... • Known: transcription factors bind to small subsequences of DNA, perhaps in a statistical mechanical (hence concentration dependent) way. • Controversial: interaction among different transcription factor-binding events. ...
... • Known: transcription factors bind to small subsequences of DNA, perhaps in a statistical mechanical (hence concentration dependent) way. • Controversial: interaction among different transcription factor-binding events. ...
Slide ()
... cetuximab, trastuzumab, or panitumumab acting at the receptor. Tyrosine kinases (TKs) that are not directly stimulated by growth factors such as p210 bcr-abl or src can be inhibited by imatinib, dasatinib, or nilotinib. Signals projected downstream from growth factor receptors can be affected by the ...
... cetuximab, trastuzumab, or panitumumab acting at the receptor. Tyrosine kinases (TKs) that are not directly stimulated by growth factors such as p210 bcr-abl or src can be inhibited by imatinib, dasatinib, or nilotinib. Signals projected downstream from growth factor receptors can be affected by the ...
The role of dSAGA specific histone acetylation in regulation of gene
... Considerable and dynamic compaction of the genome is essential for normal function of eukaryotic cells. This is carried out by the chromatin structure, in which 147 base pairs of DNA is wound around a core of four basic histone proteins (H2A, H2B, H3, H4) to form the basic building blocks, the nucle ...
... Considerable and dynamic compaction of the genome is essential for normal function of eukaryotic cells. This is carried out by the chromatin structure, in which 147 base pairs of DNA is wound around a core of four basic histone proteins (H2A, H2B, H3, H4) to form the basic building blocks, the nucle ...
Etoposide Phosphate Enhances the Acetylation Level
... Translation elongation factor 1A (eEF1A) is a factor critically involved in the process of protein synthesis. The activity of eEF1A has been shown by several studies to be regulated by post-translational modifications such as phosphorylation and dephosphorylation. However, until now less research has ...
... Translation elongation factor 1A (eEF1A) is a factor critically involved in the process of protein synthesis. The activity of eEF1A has been shown by several studies to be regulated by post-translational modifications such as phosphorylation and dephosphorylation. However, until now less research has ...
organization of chromatin and the fate of a cell
... not well understood. We now know that the fate of a cell is not just decided by the sequence of the DNA but also by the “state” of its chromatin. Chromatin is a 3-dimensional active assembly of DNA bound by many proteins (a set of biopolymer molecules). Chromatin can be assembled in multiple ways. A ...
... not well understood. We now know that the fate of a cell is not just decided by the sequence of the DNA but also by the “state” of its chromatin. Chromatin is a 3-dimensional active assembly of DNA bound by many proteins (a set of biopolymer molecules). Chromatin can be assembled in multiple ways. A ...
Notes (other NGS applications)
... High-Resolution Profiling of Histone methylations in the human genome Ref: Cell, 129(4):823-837, 2007 Generated high-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations and others across the human genome using the Solexa 1G sequencing technology (The ...
... High-Resolution Profiling of Histone methylations in the human genome Ref: Cell, 129(4):823-837, 2007 Generated high-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations and others across the human genome using the Solexa 1G sequencing technology (The ...
PINK1 positively regulates HDAC3 to suppress p53
... suppressing p53-dependent apoptosis has been recently emphasized; however, the molecular basis of modulation of p53 function by HDAC3 remains unclear. Here, we show that cytoplasmic PTEN-induced putative kinase 1 (PINK1) directly binds to and phosphorylates HDAC3 at Ser-424 to enhance its histone de ...
... suppressing p53-dependent apoptosis has been recently emphasized; however, the molecular basis of modulation of p53 function by HDAC3 remains unclear. Here, we show that cytoplasmic PTEN-induced putative kinase 1 (PINK1) directly binds to and phosphorylates HDAC3 at Ser-424 to enhance its histone de ...
Regulation of gene expression
... expression of genes → relevant differentiation status of various cell types ► cell differentiation arises because cells make and accumulate different sets of RNA and protein molecules; that is, they express different genes ...
... expression of genes → relevant differentiation status of various cell types ► cell differentiation arises because cells make and accumulate different sets of RNA and protein molecules; that is, they express different genes ...
Allosteric Modulation of DNA by Small Molecules
... Allosteric Modulation of DNA by Small Molecules Signals originating at the cell surface are conveyed by a complex system of interconnected signaling pathways to the nucleus. They converge at transcription factors, which in turn regulate the transcription of sets of genes that result in the gene expr ...
... Allosteric Modulation of DNA by Small Molecules Signals originating at the cell surface are conveyed by a complex system of interconnected signaling pathways to the nucleus. They converge at transcription factors, which in turn regulate the transcription of sets of genes that result in the gene expr ...
Team Publications - Centre de recherche de l`Institut Curie
... Role of the polycomb protein EED in the propagation of repressive histone marks. Nature : 762-7 : DOI : 10.1038/nature08398 ...
... Role of the polycomb protein EED in the propagation of repressive histone marks. Nature : 762-7 : DOI : 10.1038/nature08398 ...
Transcription Biology Review
... • Proteins that line up several amino acid contacts bind strongly to specific DNA sequences ...
... • Proteins that line up several amino acid contacts bind strongly to specific DNA sequences ...
Histone acetylation and deacetylation
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.