Inquiry into Life Twelfth Edition
... • Sumoylation is the addition of one or more copies of the 101-amino acid polypeptide SUMO (Small Ubiquitin-Related Modifier) to lysine residues on a protein • Process is similar to ubiquitylation • Results quite different – sumoylated activators are targeted to a specific nuclear compartment that k ...
... • Sumoylation is the addition of one or more copies of the 101-amino acid polypeptide SUMO (Small Ubiquitin-Related Modifier) to lysine residues on a protein • Process is similar to ubiquitylation • Results quite different – sumoylated activators are targeted to a specific nuclear compartment that k ...
Promoters
... 2. Tet-on systems: older versions – a significant basal activity; fully active only at high Dox doses novel versions: display a considerably lower basal activity in the OFF state - have codon-optimized sequence – results in improved expression and stability However, tightness of the control may be p ...
... 2. Tet-on systems: older versions – a significant basal activity; fully active only at high Dox doses novel versions: display a considerably lower basal activity in the OFF state - have codon-optimized sequence – results in improved expression and stability However, tightness of the control may be p ...
PPT File
... This then binds to a proteasome, a protein complex which contains enzymes. The polyubiquitin is removed and ATP is used to unfold the protein. It is digested into peptides and amino acids. Cyclins in the cell cycle are regulated in this way. ...
... This then binds to a proteasome, a protein complex which contains enzymes. The polyubiquitin is removed and ATP is used to unfold the protein. It is digested into peptides and amino acids. Cyclins in the cell cycle are regulated in this way. ...
Slide 1 - Elsevier
... Copyright © 2012, American Society for Neurochemistry. Published by Elsevier Inc. All rights reserved. ...
... Copyright © 2012, American Society for Neurochemistry. Published by Elsevier Inc. All rights reserved. ...
lecture13_06
... (D) signaling and angiogenesis, and (E) wound healing and tissue remodeling. These clusters also contain named genes not involved in these processes and numerous uncharacterized genes. ...
... (D) signaling and angiogenesis, and (E) wound healing and tissue remodeling. These clusters also contain named genes not involved in these processes and numerous uncharacterized genes. ...
ChIP-seq
... Transcription factors Histones (various types and modifications) RNA Polymerase (survey of transcription) DNA polymerase (investigate DNA replication) DNA repair enzymes ...
... Transcription factors Histones (various types and modifications) RNA Polymerase (survey of transcription) DNA polymerase (investigate DNA replication) DNA repair enzymes ...
Gene Section RBTN2 (rhombotin-2) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Gata-1) in the erythroid complex; this interaction is critical for the regulation of red blood cell development in early stages of hematopoiesis (mouse); because Lmo2 can also bind to GATA-2 protein, a complex Lom2-Gata2 might occur at earlier stages of hematopoiesis when Gata1 is not expressed; Lmo ...
... Gata-1) in the erythroid complex; this interaction is critical for the regulation of red blood cell development in early stages of hematopoiesis (mouse); because Lmo2 can also bind to GATA-2 protein, a complex Lom2-Gata2 might occur at earlier stages of hematopoiesis when Gata1 is not expressed; Lmo ...
Examination in Bi3016 Molecular Cell Biology
... b. Explain how transcription factors specificity and affinity to DNA can be increased. How can this also increase the number of potential binding sites for the transcription factor? c. How does the nucleosome structure affect the binding of transcription factors? How does DNA become accessible for t ...
... b. Explain how transcription factors specificity and affinity to DNA can be increased. How can this also increase the number of potential binding sites for the transcription factor? c. How does the nucleosome structure affect the binding of transcription factors? How does DNA become accessible for t ...
Chapter 8 Gene Regulation in Eukaryotes 6
... nucleosome modifiers that compact the nucleosome or remove the groups recognized by the transcriptional machinery. For example, histone deacetylases repress transcription by removing acetyl groups from the tails of histones. ...
... nucleosome modifiers that compact the nucleosome or remove the groups recognized by the transcriptional machinery. For example, histone deacetylases repress transcription by removing acetyl groups from the tails of histones. ...
Inquiry into Life Twelfth Edition
... • ChIP-chip analysis can be used to identify DNAbinding sites for activators and other proteins • Small genome organisms - all of the intergenic regions can be included in the microarray • If genome is large, that is not practical • To narrow areas of interest can use CpG islands – These are associa ...
... • ChIP-chip analysis can be used to identify DNAbinding sites for activators and other proteins • Small genome organisms - all of the intergenic regions can be included in the microarray • If genome is large, that is not practical • To narrow areas of interest can use CpG islands – These are associa ...
Diapositiva 1 - digital
... Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA recruitment to target promoters and H3 acetylation JmjC domain-containing histone demethyla ...
... Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA recruitment to target promoters and H3 acetylation JmjC domain-containing histone demethyla ...
lecture notes
... possible to convert any tissue into iPS cells (induced pluripotent stem cells) by forced expression of three transcription factors (TFs): Nanos, Oct4, and Sox2. iPS cells, in turn, can form any cell type, including cardiomyocytes, neurons, muscles, blood cells, and skin. It is therefore possible to ...
... possible to convert any tissue into iPS cells (induced pluripotent stem cells) by forced expression of three transcription factors (TFs): Nanos, Oct4, and Sox2. iPS cells, in turn, can form any cell type, including cardiomyocytes, neurons, muscles, blood cells, and skin. It is therefore possible to ...
Other Plasmid Maps Feature list descriptions
... of ampicillin. E.coli transformed with plasmid containing the ampicillin resistance gene can grow on media containing 50-100 µg/ml ampicillin. (www.jac.oxfordjournals.org/content/43/5/699.full) ...
... of ampicillin. E.coli transformed with plasmid containing the ampicillin resistance gene can grow on media containing 50-100 µg/ml ampicillin. (www.jac.oxfordjournals.org/content/43/5/699.full) ...
Post-translation
... Proteins build up all living things: plants, animals and therefore us humans. In the past few decades biochemistry has come a long way towards explaining how the cell produces all its various proteins(at least five Nobel Prizes have been awarded in this area). But as to the breaking down of protein ...
... Proteins build up all living things: plants, animals and therefore us humans. In the past few decades biochemistry has come a long way towards explaining how the cell produces all its various proteins(at least five Nobel Prizes have been awarded in this area). But as to the breaking down of protein ...
DNA to RNA
... • We’re going to make a “Protein Synthesis Tri-fold” • Follow my directions, step by step ...
... • We’re going to make a “Protein Synthesis Tri-fold” • Follow my directions, step by step ...
chapter13
... Some genes encode for proteins that are always needed. These genes are constantly transcribed. They are called constitutive genes. E.g. enzymes needed for glycolysis. Most regulated genes in bacteria are organized into operons. Operons permit coordinated control of functionally related genes. An ope ...
... Some genes encode for proteins that are always needed. These genes are constantly transcribed. They are called constitutive genes. E.g. enzymes needed for glycolysis. Most regulated genes in bacteria are organized into operons. Operons permit coordinated control of functionally related genes. An ope ...
Constructing and analyzing disease
... Institute for Biostatistics and Informatics in Medicine and Ageing Research Rostock -‐ Germany ...
... Institute for Biostatistics and Informatics in Medicine and Ageing Research Rostock -‐ Germany ...
Gene Section FOXA1 (forkhead box A1) Atlas of Genetics and Cytogenetics
... domain in a winged helix-loop-helix configuration. Both N-terminus and C-terminus have transactivation domains. In silico analysis revealed 11 putative acetylation sites; acetylation sites in the DNA binding domain inhibit interaction with chromatin. The Nterminus has a putative caseine kinase 1 pho ...
... domain in a winged helix-loop-helix configuration. Both N-terminus and C-terminus have transactivation domains. In silico analysis revealed 11 putative acetylation sites; acetylation sites in the DNA binding domain inhibit interaction with chromatin. The Nterminus has a putative caseine kinase 1 pho ...
Protein Structure-Function Relationships - IBIVU
... The DNA-binding motif is found as part of transcription regulatory proteins. ...
... The DNA-binding motif is found as part of transcription regulatory proteins. ...
Lysine Acetylation - Regulator of Diverse Cellular Processes
... crucial for regulating the function and localization of many eukaryotic proteins. This PTM is reversible, regulated by histone deacetylases (HDACs) and histone acetyltransferases (HATs). The first evidence of lysine acetylation was discovered in calf thymus histones in 19681. In the following decade ...
... crucial for regulating the function and localization of many eukaryotic proteins. This PTM is reversible, regulated by histone deacetylases (HDACs) and histone acetyltransferases (HATs). The first evidence of lysine acetylation was discovered in calf thymus histones in 19681. In the following decade ...
Gene to Protein
... DNA – forms Hydrogen bonds and reforms double helix mRNA is edited (remove introns, exons are to be expressed) mRNA leaves the nucleus and enters cytoplasm for translation ...
... DNA – forms Hydrogen bonds and reforms double helix mRNA is edited (remove introns, exons are to be expressed) mRNA leaves the nucleus and enters cytoplasm for translation ...
April 3 lecture slides
... polymerase complex are names “enhanceosomes”. TBP stands for TATAbinding protein, a component of RNA polymerase II associated factor, TFIID ...
... polymerase complex are names “enhanceosomes”. TBP stands for TATAbinding protein, a component of RNA polymerase II associated factor, TFIID ...
ExPlain: Causal Analysis of Gene Expression Data from Promoter
... in providing fast and appropriate response to any extracellular signal. This is achieved through combinatorial usage of a rather limited set of signaling molecules and pathways. These combinatorics must be mirrored by the structure of gene promoters as combinations of transcription factor binding si ...
... in providing fast and appropriate response to any extracellular signal. This is achieved through combinatorial usage of a rather limited set of signaling molecules and pathways. These combinatorics must be mirrored by the structure of gene promoters as combinations of transcription factor binding si ...
Histone acetylation and deacetylation
Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. Histone acetylation and deacetylation are essential parts of gene regulation. These reactions are typically catalysed by enzymes with ""histone acetyltransferase"" (HAT) or ""histone deacetylase"" (HDAC) activity. Acetylation is the process where an acetyl functional group is transferred from one molecule (in this case, Acetyl-Coenzyme A) to another. Deacetylation is simply the reverse reaction where an acetyl group is removed from a molecule.Acetylated histones, octameric proteins that organize chromatin into nucleosomes and ultimately higher order structures, represent a type of epigenetic marker within chromatin. Acetylation removes the positive charge on the histones, thereby decreasing the interaction of the N termini of histones with the negatively charged phosphate groups of DNA. As a consequence, the condensed chromatin is transformed into a more relaxed structure that is associated with greater levels of gene transcription. This relaxation can be reversed by HDAC activity. Relaxed, transcriptionally active DNA is referred to as euchromatin. More condensed (tightly packed) DNA is referred to as heterochromatin. Condensation can be brought about by processes including deacetylation and methylation; the action of methylation is indirect and has no effect upon charge.