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A Pneumocystis carinii multi-gene family with
A Pneumocystis carinii multi-gene family with

... specific endoproteolytic activity of some of them has been elucidated. However, for many others, the precise biological function has not yet been determined. In this paper we report the identification and characterization of the P. carinii f. sp. carinii PRTl multigene family. We demonstrate high le ...
View PDF - OSU Biochemistry and Molecular Biology
View PDF - OSU Biochemistry and Molecular Biology

... required for appendage formation, encodes a homeobox transcription factor and is one of the high-level executives regulated by Hox proteins. The area of Dll expression in the embryo (shown as grey patches) corresponds to sites where in the course of development imaginal discs and, subsequently, appe ...
The Origin and Development of Neural Crest
The Origin and Development of Neural Crest

... After the neural crest cells leave the migration staging area, they migrate either ventrally or dorsal-laterally away from the neural tube (Fig. 1, no. 2). In mice, these migratory events appear to occur simultaneously, whereas, in chick, dorsal–lateral migration of melanoblasts appears to be delaye ...
UNIT – I: NUCLEIC ACID AND PROTEIN SYNTHESIS AND
UNIT – I: NUCLEIC ACID AND PROTEIN SYNTHESIS AND

... base, uracil , which can also base pair with adenine In addition, RNA molecules normally exist as a single polynucletide strand and do not form a double helix. However, it is possible for base pairing to occur between complementary parts of the same RNA strand resulting in short double stranded regi ...
ABO Blood Group System
ABO Blood Group System

... The ABO Blood Group System was the first to be identified and is the most significant for transfusion practice It is the ONLY system that the reciprocal antibodies are consistently and predictably present in the sera of people who have had no exposure to human red cells ...
WUS and STM in shoot meristem regulation
WUS and STM in shoot meristem regulation

... phenotype, STM appears to maintain cells in an undifferentiated state, before they are incorporated into leaf primordia. To test whether STM was sufficient to suppress differentiation, we expressed STM ectopically in leaf primordia, using the pOpL two-component system (Moore et al., 1998; see Materi ...
(F193L) in the KCNQ1 gene associated with long
(F193L) in the KCNQ1 gene associated with long

... KCNQ1 is a gene encoding an α subunit of voltage-gated cardiac K+ channels, with properties similar to the slowly activating delayed rectifier K+ current, and one of the genes causing long QT syndrome (LQTS). However, genotype–phenotype correlations of the KCNQ1 gene mutations are not fully understo ...
Histones - scientia.global
Histones - scientia.global

... many of the factors that are required for histone mRNA biosynthesis. As mentioned above, the protein FLASH and U7 snRNP are components of the HLB that participate in 3′ processing of the nonpolyadenylated histone mRNAs by recruiting the endonuclease CPSF-73 to the histone pre-mRNA. Together with Pro ...
Genome-Wide Scan of Obesity in the Old Order Amish*
Genome-Wide Scan of Obesity in the Old Order Amish*

... same BMI. BMI-adjusted leptin levels were highly correlated with both percent fat (r ⫽ 0.60) and the original (i.e. unadjusted) leptin concentrations (r ⫽ 0.76), suggesting a potential link between BMI-adjusted leptin levels and obesity. The heritabilities for BMI, waist circumference, percent fat, ...
Antibodies Instructional Module
Antibodies Instructional Module

... Pepsin is a digestive protease created by stomach cells that degrade food proteins. Papain is a cysteine protease, commonly found in papaya, also can digest proteins. As a result of using either, the protein-based antibodies can be broken into fragments. The tips of the Fab region, composed of heavy ...
Pultz, M. A., and Baker, B. S.
Pultz, M. A., and Baker, B. S.

... effects. We have also taken advantage of the herl(2)mat allele (Redfield, 1926), which is severely defective in maternal her functions but comparable to wild-type in zygotic her functions (Pultz et al., 1994). Maternal effects of her on female viability The maternal function of her is needed for the ...
Input signals to the plant circadian clock
Input signals to the plant circadian clock

... plant physiology, some of which are obvious (such as the `sleep movements' of legume leaves, noted since classical times), others less so. In several cases, genes that affect a common pathway or process are expressed at the same phase, suggesting that the phase might be important for the function of ...
Pathways to abscisic acid-regulated gene expression
Pathways to abscisic acid-regulated gene expression

... expression, from the perspective of the bench scientist. There are, broadly speaking, three complementary experimental approaches to the study of fundamental biological questions, each with its advantages and disadvantages : (1) classical genetics, (2) the broad and loosely termed ‘ reverse genetics ...
Evaluation of TMPRSS2-ERG Fusion Protein in
Evaluation of TMPRSS2-ERG Fusion Protein in

... www.biocare.net ERG100 ...
Experimental and Molecular Pathology
Experimental and Molecular Pathology

... specific to epithelial cells (Brisette et al. 1996). Foxn1 expression in the thymus is also restricted to epithelial cells and has been shown to be necessary for the development of the mature thymic epithelium (Blackburn et al., 1996; Brisette et al., 1996; Lee et al., 1999). Expression of the Foxn1 ...
Foundations of Biology - Geoscience Research Institute
Foundations of Biology - Geoscience Research Institute

... The authors who reported the presence of mitochondrial genes in amitochondrial eukaryotes all reinterpreted prevailing theory in saying that mitochondria must have been present then lost after they had transferred some of their genetic information to the nucleus. The hydrogenosome, a structure invol ...
Engineered bacteriophage-defence systems in bioprocessing
Engineered bacteriophage-defence systems in bioprocessing

... REVIEWS In addition, two DNA-replication modules have been identified among S. thermophilus phages: Sfi21type and 7201-type. The Sfi21-type replication module is composed of a single origin of DNA replication (ori) and several open reading frames that encode a putative helicase, a putative primase ...
Mutations in type I collagen genes resulting in osteogenesis
Mutations in type I collagen genes resulting in osteogenesis

... encode the chains of type I procollagen. OI is associated with a wide spectrum of phenotypes varying from mild to severe and lethal conditions. The mild forms are usually caused by mutations which inactivate one allele of COL1A1 gene and result in a reduced amount of normal type I collagen, while th ...
Mendelian Genetics Chapter 12 Reading Mendellian Genetics
Mendelian Genetics Chapter 12 Reading Mendellian Genetics

... sativum, shown in Figure 1. Farmers had done similar experiments before, but Mendel was the first person to develop rules that accurately predict the patterns of heredity in pea plants. V Modern genetics is based on Mendel’s explanations for the patterns of heredity in garden pea plants. As a young ...
Mutant Fruit Flies: Exploratorium Exhibit. Mutations in
Mutant Fruit Flies: Exploratorium Exhibit. Mutations in

... melanogaster. (In our real exhibit you'd be looking at the actual flies crawling around, looking for food or grooming their wings.) Compare the mutated flies to the normal flies. ...
Glutathione production by efficient ATP
Glutathione production by efficient ATP

... (Hara & Mori, 2006), and have systematically constructed a genome-wide single-gene deletion Escherichia coli K-12 library, termed as the ‘Keio Collection’ (Baba et al., 2006). A combination of the Permeable Cell Assay and the Keio Collection made it possible to identify, on a genome-wide basis, the ...
The Serratia gene cluster encoding biosynthesis of the red antibiotic
The Serratia gene cluster encoding biosynthesis of the red antibiotic

Reciprocal deletion and duplication at 2q23.1 indicates a
Reciprocal deletion and duplication at 2q23.1 indicates a

... recommended by the manufacturer. Relative differences in transcript levels were quantified according to the DDCt method and normalized to GAPDH. Data were analyzed with 7500 Fast System SDS Software (Applied Biosystems). Standard error was generated for each sample as previously described, and signi ...
X-adrenoleukodystrophy
X-adrenoleukodystrophy

... genotypes of the mice that were used in this study. The first genotype they tested was the generation of ALDR-/-, inactivation of the ALDR gene. This leads to an absence of the ALDR protein, and was observed through the use of a western blot. The ALDR-/- mice were fertile and they showed no physical ...
AT3 (Acyltransferase) Gene Isolated from Capsicum frutescens cv
AT3 (Acyltransferase) Gene Isolated from Capsicum frutescens cv

... not yet been isolated. Furthermore, there is another fragment downstream from amino acid 140 th that has not yet been obtained in our study. So far, there is no report for the exact length of AT3 gene from C. frutescens cv. Shuanla [15] which shows no stop codon in their reported AT3 sequence. ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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