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alpha-amylase from bacillus licheniformis
alpha-amylase from bacillus licheniformis

... liquefaction in the production of nutritive sweeteners from starch. The genetically engineered α-amylase is manufactured by pure culture fermentation of the nonpathogenic and nontoxigenic Bacillus licheniformis production strain. The enzyme is subsequently purified, concentrated, and formulated with ...
Direct control of shoot meristem activity by a cytokinin
Direct control of shoot meristem activity by a cytokinin

... escence is measured, presumably owing to feedback regulation (data not shown). The determination of local cytokinin contents in the tip of the shoot meristem, where LOG is expressed (Fig. 3), is practically impossible due to the small number of LOG-expressing cells. Thus, as an alternative way to ex ...
Presentation - people.vcu.edu
Presentation - people.vcu.edu

... Myelin-associated glycoprotein (MAG) Acts as a linker between the axon and the myelin sheath ...
Novel genetic aspects of Klinefelter`s syndrome
Novel genetic aspects of Klinefelter`s syndrome

... years, new screening methods have been published: fluorescence in situ hybridization (FISH) may be used to estimate mosaicism in more detail by analyzing a larger number of interphase nuclei (Abdelmoula et al., 2004; Lenz et al., 2005). The extra X can also be detected by quantitative real-time PCR ( ...
Progress in the Understanding of the Genetic Etiology of Vertebral
Progress in the Understanding of the Genetic Etiology of Vertebral

... of CVM is also encountered and includes hemivertebrae, vertebral bars, supernumerary vertebrae, and butterfly and wedge-shaped vertebrae.1–2 Genetic transmission in some cases of CVM has been documented with frequency of familial recurrence rate estimated at 3%. Thus, with a lack of evidence to sugg ...
Chip-seq analysis
Chip-seq analysis

... Exonic, intronic, intergenic, promoter, bidirectional promoter, known epigenetic marks, any user defined regions (...)? ...
Genome Projector: zoomable genome map with multiple views
Genome Projector: zoomable genome map with multiple views

... biological context for the visualisation and interpretation of data, and serve as a gateway to information stored in a database [2]. For example, the popular two-dimensional genome map viewer, Gbrowse, provides an entry point for browsing the model organism genome database, GMOD [3], and biochemical ...
Handbook on SMA genetics_final_051209
Handbook on SMA genetics_final_051209

... Both SMN1 and SMN2 contain nine exons and are very similar. Their sequences differ only in five nucleotides (three are intronic and two are exonic, located within exons 6, 7, and 8). (See Biros and Forrest J Med Genetics 1999; 36:1-8.) However, only the nucleotide difference in exon 7 is of function ...
Conjugative plasmids: vessels of the communal gene pool
Conjugative plasmids: vessels of the communal gene pool

... between discrete replicating elements (chromosomes or plasmids) by the movement of relatively small fragments of DNA. Phylogenetic analyses of complete genomes have also shown that some prokaryotic genes are more likely to be transferred than others (Lawrence & Hendrickson 2003), which, by deduction ...
Conjugative plasmids: vessels of the communal gene pool
Conjugative plasmids: vessels of the communal gene pool

... between discrete replicating elements (chromosomes or plasmids) by the movement of relatively small fragments of DNA. Phylogenetic analyses of complete genomes have also shown that some prokaryotic genes are more likely to be transferred than others (Lawrence & Hendrickson 2003), which, by deduction ...
The Structural, Biochemical, and Genetic Characterization of a New
The Structural, Biochemical, and Genetic Characterization of a New

... The r-m allele of the R locus of soybean affects seed coat pigmentation patterns (Chandlee and Vodkin, 1989a). A variegated distribution of black spots and/or concentric rings of pigment on an otherwise brown seed coat is produced by somatic mutations of the mutable allele. The r-m allele exhibits i ...
Heredity and Math - Computer Science
Heredity and Math - Computer Science

... Discuss eye color variations and the fact that dark is dominant and light is recessive. Assign a value from 1-10 for each student’s eye color, with 1 being very light and 10 being black. Create a frequency table, line plot or bar graph of the class results. Work in pairs to answer worksheet question ...
Melanocortin 1 recep
Melanocortin 1 recep

... invasive species (Gurnell et al. 2004). These British grey squirrels have three distinct colour morphs; wildtype grey, brown-black and jet black. Brown-black and jet black morphs are both considered to be melanic. The first recorded sighting of a melanic grey squirrel in the British Isles was in Bed ...
user guide here
user guide here

... gVCF. These records use the standard 'END' INFO key to indicate the extent of the record. Even though the record can span multiple bases, only the first base is provided in the REF field to reduce file size. The following is a simplified segment of a gVCF file, describing a segment of non-variant ca ...
A large scale analysis of resistance gene
A large scale analysis of resistance gene

... A. thaliana for resistance to Pseudomonas syringae (gi 15221252), R-gene Mi-1 against nematodes and aphids from Lycopersicon esculentum (gi 7489037) and gene Rpp 8 of A. thaliana (gi 17064876). The sequence of primer RNBS-D-rev was kindly provided by Nevin Young. Primer LM638 was developed by Kanazi ...
Transgenic Tobacco Plant Expressing Environmental E. coli merA
Transgenic Tobacco Plant Expressing Environmental E. coli merA

... transgenic tobacco expressing merA volatilized significantly more mercury than wild-type plants. This is first time we are reporting the expression of a bacterial native merA gene via the nuclear genome of Nicotiana tabacum, and enhanced mercury volatilization from tobacco transgenics. The study cle ...
Genetics PowerPoint - Lewiston School District
Genetics PowerPoint - Lewiston School District

... that those traits would show in their offspring. He discovered that if there were different traits in the plants’ history, those traits might eventually show up. ...
Genetics Questions - G. Holmes Braddock
Genetics Questions - G. Holmes Braddock

... ____ 18. Use Figure 11–3 to answer the following question. If a pea plant that is heterozygous for round, yellow peas (RrYy) is crossed with a pea plant that is homozygous for round peas but heterozygous for yellow peas (RRYy), how many different phenotypes are their offspring expected to show? a. 2 ...
Molecular Imaging - Engineering Computing Facility
Molecular Imaging - Engineering Computing Facility

... of the tumour for deciding whether it is benign or not and how to treat it. Instead, its type, distribution, gene expression and cellular functions are the driving decision parameters for setting the method of treatment, if it should be treated at all [1]. Another problem with current imaging techno ...
Molecular cloning and biochemical characterization of a
Molecular cloning and biochemical characterization of a

... In order to better characterize the microsomal and soluble LOX, we analyzed the reaction products obtained using linoleic acid as substrate and microsomal and soluble fractions purified from seeds at 30 DAF as sources of LOX. After reduction with sodium borohydride, reaction products were first sepa ...
Role of tumor necrosis factor alpha gene polymorphism in childhood
Role of tumor necrosis factor alpha gene polymorphism in childhood

... Bronchial asthma is a common disease with multiple determinants that include genetic variation, environmental exposures, and gene–environment interactions. Tumor necrosis factor alpha (TNF-α) has a role in asthma and wheezing pathophysiology. Single nucleotide gene polymorphisms, may be important as ...
The slime strain was grown in Nelson B  medium... for 22 hours. HCH stereoisomers were dissolved in 83% ethanol...
The slime strain was grown in Nelson B medium... for 22 hours. HCH stereoisomers were dissolved in 83% ethanol...

... Centromere distances and linkage relationships of ascospore color mutants. Centromere distances are useful to know and are readily determined for ascospore mutants. First- (MI) and second- (MII) division segregation frequencies are shown in Table 2. Asci were scored microscopically without isolating ...
Association of Apolipoprotein E Alleles with Susceptibility to Age
Association of Apolipoprotein E Alleles with Susceptibility to Age

... is no gender-specific effect for ε2 and both sexes have the same risk of development of this disease. On the other hand, although several studies have shown that APOE ε4 allele protects against development of AMD (16, 21, 24), our results do not provide any suggestive evidence for this protection. T ...
Mutations in type I collagen genes resulting in osteogenesis
Mutations in type I collagen genes resulting in osteogenesis

... encode the chains of type I procollagen. OI is associated with a wide spectrum of phenotypes varying from mild to severe and lethal conditions. The mild forms are usually caused by mutations which inactivate one allele of COL1A1 gene and result in a reduced amount of normal type I collagen, while th ...
Nucleus Evidence 1
Nucleus Evidence 1

...  In healthy people, the DRB gene gives instructions to the cells to produce a protein called the DRB protein. (Proteins are one kind of chemical structure in cells.) The DRB protein helps make sure the body can produce insulin, so that people do not get diabetes.  When people have a mutation in th ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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