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High-Resolution Single-Copy Gene Fluorescence in Situ
High-Resolution Single-Copy Gene Fluorescence in Situ

... been distinctly illustrated by the molecular and cytological characterization of a heterochromatic knob on the short arm of Arabidopsis thaliana chromosome 4 (Fransz et al., 2000) and the confirmation of anchored BAC contigs and gene orders in rice (Oryza sativa) (Zhao et al., 2002). In maize, howev ...
Lesson Overview
Lesson Overview

... How do alleles segregate when more than one gene is involved? The principle of independent assortment states that genes for different traits can segregate independently during the formation of gametes. ...
phenotype describes a new mutation affecting
phenotype describes a new mutation affecting

... The ABA content of embryos and leaves was determined by radioimmunoassay using DBPA1 monoclonal antibody as previously described (Bochicchio et al., 1994). Each analysis was performed in triplicate on pools of six embryos. Since there is no difference in mutant versus wild-type embryo size, the resu ...
Pedigree analysis through genetics hypothesis testing
Pedigree analysis through genetics hypothesis testing

... Hypothesis 4: Autosomal recessive Look at the family with individuals 3 and 4 as parents. Individual 3 could be homozygous for the nonmutant allele or heterozygous. Individual 4 has only nonmutant alleles. Regardless of whether the mother is homozygous for the nonmutant allele or heterozygous, they ...
Fitness landscapes of gene regulation in variable - UvA-DARE
Fitness landscapes of gene regulation in variable - UvA-DARE

... with the optimal Pgal concentrations as obtained directly from the growth data in figure 6.1. Although the optimal level shows a very sharp Pgal dependence, this does not mean that the growth difference for optimal and non-optimal expression are necessarily large. We can see from the landscape in fig. ...
Genetic analysis of the floral initiation process (FLIP) in Arabidopsis
Genetic analysis of the floral initiation process (FLIP) in Arabidopsis

... and C genes are mutually exclusive, such that each gene class restricts the expression of the other. Class A activity also appears to influence Class B expression, since carpels can be found in the second and third whorls of strong AP2 alleles (Kunst et al., 1989; Haughn et al., 1993) and AP3 transc ...
Marfan syndrome
Marfan syndrome

... type profibrillin is not incorporated into extracellular matrix until it is converted to fibrillin 24. ...
Gene Section ALK (anaplastic lymphoma kinase) Atlas of Genetics and Cytogenetics
Gene Section ALK (anaplastic lymphoma kinase) Atlas of Genetics and Cytogenetics

... 680 amino acids, 80 kDa; N-term 116 amino acids from NPM1 fused to the 562 C-term aminoacids of ALK (i.e. composed of the oligomerization domain and the metal binding site of NPM1, and the entire cytoplasmic portion of ALK); no apparent expres-sion of the ALK/NPM1 counterpart. Characteristic localis ...
Massive Changes in Genome Architecture Accompany
Massive Changes in Genome Architecture Accompany

... The suppression of recombination across such a region will be selected for if it creates linkage between the sex-determining locus and other genes that are sexually antagonistic in that their functions are beneficial to only one of the sexes. The nonrecombining region can be formed from the spread of ...
the kinship theory of genomic imprinting - Fischer Lab
the kinship theory of genomic imprinting - Fischer Lab

... Two different factors of one half have entered into traditional calculations of relatedness (23, 24, 85). The first arises when calculating forward from parent to offspring and reflects the random nature of meiotic segregation. The second arises when calculating backward from offspring to parent and ...
Proceedings as -file
Proceedings as -file

... ƒ Comparison test: Comments from Duty Laboratory South Africa; Comments from Computing Laboratory The Netherlands; Discussion ƒ ISAG panels: Do they work well? How many laboratories are using these panels in the routine? Do we need changes? Standard / Reference samples? • Nomenclature: Is there a ne ...
Creating conditional dual fluorescence labelled transgenic animals
Creating conditional dual fluorescence labelled transgenic animals

... critical to the field. We developed a fast and straightforward method to determine ncRNAtransgene copy number, orientation, and insertion site in the genome. Furthermore, upon tissuespecific expression of ncRNA, a Cre-loxP mediated dual-fluorescence expression system facilitates fluorescence signal ...
6.3 Mendel and Heredity
6.3 Mendel and Heredity

... • Female mammals have an XX genotype – X-inactivation – one X chromosome is randomly “turned off” in every cell – Ex: calico cats ...
Isolation of insertion elements from Gram
Isolation of insertion elements from Gram

... was shown that they carry the unaffected vector pWJ5. This was shown by introducing the plasmids in question into E. coli and by testing for sucrose sensitivity (data not shown). Therefore, besides mutation of the sacB gene, at least one other, so far unknown resistance mechanism should exist. Since ...
illuminaHumanv3.db October 28, 2014
illuminaHumanv3.db October 28, 2014

... indicates the chromosome. Due to inconsistencies that may exist at the time the object was built, these vectors may contain more than one chromosome and/or location. If the chromosomal location is unknown, the vector will contain an NA. Chromosomal locations on both the sense and antisense strands a ...
Systematic and Applied Microbiology
Systematic and Applied Microbiology

... Koser citrate medium and were non-motile. Adonitol is generally utilized and fermented by Klebsiella [18] but the three banana isolates F2R9T, 6A2 and VI, strain T29A from sugar cane, strain 3 from maize, strain CFNE 2006 from rice and 7 of the 131 clinical isolates (5.3% of the total) did not ferme ...
Genetic Homologies between Flagellar Antigens of
Genetic Homologies between Flagellar Antigens of

... surface antigens, flagellar as well as cell wall. Immunological cross-reactions exist between various species (or serotypes) of a genus, and also between different genera. This holds true especially of the somatic (polysaccharide) antigens of Escherichia, Salmonella, Arizona, and Citrobacter (Westph ...
BRAIN Clinical and genetic diversity of SMN1-negative proximal spinal muscular atrophies
BRAIN Clinical and genetic diversity of SMN1-negative proximal spinal muscular atrophies

... muscle atrophy may be helpful. Severe forms of the disorder can be lethal from an early age due to respiratory insufficiency. Patients with milder forms are disabled due to muscle weakness and wasting, and they may eventually become wheelchair-bound. Thus, there is an urgent need to establish a more ...
SNP Analysis (GAW15 data)
SNP Analysis (GAW15 data)

... Chromosome 6: known strong genetic factors conforms well to the expected frequency of Hardy-Weinberg disequilibrium with this large set of markers. ...
A futile metabolic cycle activated in adipocytes by - Zen-Bio
A futile metabolic cycle activated in adipocytes by - Zen-Bio

Spectrum of [beta] thalassemia mutations and HbF levels in the
Spectrum of [beta] thalassemia mutations and HbF levels in the

... triggering the bone marrow to produce immature erythrocytes. This is associated in the patient with variable degrees of anemia, bone marrow hyperplasia, splenomegaly, and other clinical features related to the severity of the anemic state. The synthesis of HbF moderates the ␣/non-␣ globin chain imba ...
Blueprint of life
Blueprint of life

... the gametes (sex cells that form offspring). ...
Coloured Southdown Sheep - Twisty Tree Ranch Fiber Farm
Coloured Southdown Sheep - Twisty Tree Ranch Fiber Farm

... In recent years, the frequency of coloured lambs appearing in British Southdown flocks, although not high, has been increasing. This re-emergence of the coloured gene is apparently associated with the Southdown sheep imported from New Zealand in 1980 since many black lambs can be shown to be descend ...
Galactosemia screening when?
Galactosemia screening when?

... galactosemia known as type III or galactose epimerase deficiency. ...
Potential virulence and antimicrobial susceptibility of
Potential virulence and antimicrobial susceptibility of

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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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