Journal of Bacteriology
... and oIPCR2, which face outwards from the transposon sequence. The IPCR products were purified using a PCR purification kit (Amersham, United Kingdom) and sequenced using primer oIPCR3. Bioassay for inhibitory activity. LB agar (20 ml) was mixed with 0.5 ml of the indicator strain (OD at 600 nm [OD60 ...
... and oIPCR2, which face outwards from the transposon sequence. The IPCR products were purified using a PCR purification kit (Amersham, United Kingdom) and sequenced using primer oIPCR3. Bioassay for inhibitory activity. LB agar (20 ml) was mixed with 0.5 ml of the indicator strain (OD at 600 nm [OD60 ...
Bean Bunny Evolution
... so that each partner has a chance to select the beans and record the results. 11. Determine the gene frequency of F and f for each generation and record them in the chart in the columns labeled “Gene Frequency F” and “Gene Frequency f.” To find the gene frequency of F, divide the number of F by the ...
... so that each partner has a chance to select the beans and record the results. 11. Determine the gene frequency of F and f for each generation and record them in the chart in the columns labeled “Gene Frequency F” and “Gene Frequency f.” To find the gene frequency of F, divide the number of F by the ...
December 8, 2006 - Brandeis University
... Homologs of human genes involved in life-threatening diseases are useful to study when trying to understand the function or regulation of their mammalian counterparts. Experiments that purposely mutate genes to observe the ensuing effects, while not always possible in humans, are uncontroversial whe ...
... Homologs of human genes involved in life-threatening diseases are useful to study when trying to understand the function or regulation of their mammalian counterparts. Experiments that purposely mutate genes to observe the ensuing effects, while not always possible in humans, are uncontroversial whe ...
A Review on Epidermal Hair and It`s Breeding Project in Plants
... the results of complementary experiments conducted on the ttg1 mutant in A. thaliana, researchers found that the TTG1 gene in the DH line of Chinese cabbages with black seeds and epidermal hair instead of the TTG1 gene in the other DH line of Chinese cabbages with yellow seeds and no epidermal hair, ...
... the results of complementary experiments conducted on the ttg1 mutant in A. thaliana, researchers found that the TTG1 gene in the DH line of Chinese cabbages with black seeds and epidermal hair instead of the TTG1 gene in the other DH line of Chinese cabbages with yellow seeds and no epidermal hair, ...
Protein A gene expression is regulated by DNA supercoiling which
... high osmolarity alters the degree of plasmid DNA supercoiling, we looked at the effect of changing the degree of DNA supercoiling on protein A gene expression. We first determined the concentration of novobiocin, a DNA gyrase inhibitor, which relaxes DNA. Strain RN6390BF containing pE194 was grown a ...
... high osmolarity alters the degree of plasmid DNA supercoiling, we looked at the effect of changing the degree of DNA supercoiling on protein A gene expression. We first determined the concentration of novobiocin, a DNA gyrase inhibitor, which relaxes DNA. Strain RN6390BF containing pE194 was grown a ...
gmod-update-07nov - IUBio Archive for Biology
... to grow, plus long-term stability. • Data Integration: key component of Chado, public and lab data sets can be combined. • Support: shared responsibility among the GMOD community. http://eugenes.org/gmod/docs/gmod-intro-07oct.pdf ...
... to grow, plus long-term stability. • Data Integration: key component of Chado, public and lab data sets can be combined. • Support: shared responsibility among the GMOD community. http://eugenes.org/gmod/docs/gmod-intro-07oct.pdf ...
Understanding the Scurred condition in Polled Cattle
... Understanding the Scurred condition in Polled Cattle Most calves that are horned can be identified at birth or shortly after, and almost always by weaning time. Distinguishing between smooth-polled and scurred-polled is more difficult. Scurs, a rudimentary horn growth, are often not seen until cattl ...
... Understanding the Scurred condition in Polled Cattle Most calves that are horned can be identified at birth or shortly after, and almost always by weaning time. Distinguishing between smooth-polled and scurred-polled is more difficult. Scurs, a rudimentary horn growth, are often not seen until cattl ...
A genome-wide association scan in pig identifies novel regions
... selection, and the linkage QTL intervals are generally wide and therefore are not suitable for candidate gene searches. However, association mapping (also known as linkage disequilibrium mapping) with dense SNP markers can map QTL segregating in a population, and as it uses historic recombinations, ...
... selection, and the linkage QTL intervals are generally wide and therefore are not suitable for candidate gene searches. However, association mapping (also known as linkage disequilibrium mapping) with dense SNP markers can map QTL segregating in a population, and as it uses historic recombinations, ...
View PDF - G3: Genes | Genomes | Genetics
... Lethal hybrid rescue (Lhr) on chromosome II, interact to cause this lethality. Loss-of-function mutations in either gene suppress lethality, but several pieces of evidence suggest that additional factors are required for hybrid lethality. Here we screen the D. melanogaster autosomal genome by using ...
... Lethal hybrid rescue (Lhr) on chromosome II, interact to cause this lethality. Loss-of-function mutations in either gene suppress lethality, but several pieces of evidence suggest that additional factors are required for hybrid lethality. Here we screen the D. melanogaster autosomal genome by using ...
Semantic resources for biomedical data integration: phenotypes
... The biomedical knowledge domain provides lot of ontologies and electronic data bases. ...
... The biomedical knowledge domain provides lot of ontologies and electronic data bases. ...
Thalassemias
... contain α-chain inclusions that interfere with their passage through the RES, particularly the spleen. The degradation products of excess α-chains, particularly heme and iron, produce deleterious effects on red cell membrane proteins and lipids. The end result is an extremely rigid red cell with a ...
... contain α-chain inclusions that interfere with their passage through the RES, particularly the spleen. The degradation products of excess α-chains, particularly heme and iron, produce deleterious effects on red cell membrane proteins and lipids. The end result is an extremely rigid red cell with a ...
The Role of Six1 in the Genesis of Muscle Cell and Skeletal Muscle
... critical factor for skeletal muscle development since its expression is not restricted to the muscles, even though it is weakly expressed in skeletal muscles from E13.5 to E16.0 [14]. A subsequent study showed that Six1 was exclusively expressed in human adult skeletal muscle [15], which is consiste ...
... critical factor for skeletal muscle development since its expression is not restricted to the muscles, even though it is weakly expressed in skeletal muscles from E13.5 to E16.0 [14]. A subsequent study showed that Six1 was exclusively expressed in human adult skeletal muscle [15], which is consiste ...
Intellectual property rights and innovation: Evidence from
... From this empirical context, I construct three research designs to test for the effects of Celera’s IP on subsequent scientific research and product development outcomes. The first research design tests whether genes that ever had Celera’s IP differ in subsequent innovation, as of 2009, from genes ...
... From this empirical context, I construct three research designs to test for the effects of Celera’s IP on subsequent scientific research and product development outcomes. The first research design tests whether genes that ever had Celera’s IP differ in subsequent innovation, as of 2009, from genes ...
Gene Regulation and Systems Biology GA
... simulate natural genetic operators,20–22 they are useful when the closed-form optimization technique can not be applied. Further, because the GA-based design algorithm can simultaneously evaluate many points in the kinetic parameter space, it is more likely to converge toward the globally optimal t ...
... simulate natural genetic operators,20–22 they are useful when the closed-form optimization technique can not be applied. Further, because the GA-based design algorithm can simultaneously evaluate many points in the kinetic parameter space, it is more likely to converge toward the globally optimal t ...
Identification of loci affecting teat number by
... QTLs have been identified for this trait on almost all pig chromosomes except for SSC13, suggesting that teat number is controlled by multiple genes with generally small effects. We have previously identified 11 genome-wide significant QTLs for teat number by a genome scan on the White Duroc×Erhuali ...
... QTLs have been identified for this trait on almost all pig chromosomes except for SSC13, suggesting that teat number is controlled by multiple genes with generally small effects. We have previously identified 11 genome-wide significant QTLs for teat number by a genome scan on the White Duroc×Erhuali ...
WEREWOLF, a Regulator of Root Hair Pattern
... Additionally, the chromatin-associated proteins, TERMINAL FLOWER2 and EARLY BOLTING IN SHORT DAYS, repress FT transcription by direct binding in FT chromatin (Piñeiro et al., 2003; Takada and Goto, 2003). In addition, CURLY LEAF and FERTILIZATION INDEPENDENT ENDOSPERM, the subunits of Arabidopsis P ...
... Additionally, the chromatin-associated proteins, TERMINAL FLOWER2 and EARLY BOLTING IN SHORT DAYS, repress FT transcription by direct binding in FT chromatin (Piñeiro et al., 2003; Takada and Goto, 2003). In addition, CURLY LEAF and FERTILIZATION INDEPENDENT ENDOSPERM, the subunits of Arabidopsis P ...
A Very Short course in - NZ RED DEVON CATTLE BREEDERS
... none are homozygous. In fact it is impossible to produce a homozygous polled bull from this particular match. So all the offspring can pass on the gene for horns. ……But at least it’s a start. Now lets put Rotokawa 667, a great Kiwi polled bull, over your magnificent polled heifer that you paid $2,0 ...
... none are homozygous. In fact it is impossible to produce a homozygous polled bull from this particular match. So all the offspring can pass on the gene for horns. ……But at least it’s a start. Now lets put Rotokawa 667, a great Kiwi polled bull, over your magnificent polled heifer that you paid $2,0 ...
Candidate gene scan for Single Nucleotide Polymorphisms involved
... facial features, such as cephalic index, bizygomatic distance and nasal area measurements [9- ...
... facial features, such as cephalic index, bizygomatic distance and nasal area measurements [9- ...
Reduced expression of the SHORT-ROOT gene increases the rates
... required for proper root pattern formation, providing further evidence of a direct molecular link between these key developmental regulators and genes involved in cell-cycle progression [17]. AtSHR has therefore been shown to be important roles in regulating both cell division and cell fate determin ...
... required for proper root pattern formation, providing further evidence of a direct molecular link between these key developmental regulators and genes involved in cell-cycle progression [17]. AtSHR has therefore been shown to be important roles in regulating both cell division and cell fate determin ...
CapeTownGenomes
... Measure incorporation of a nucleotide using the light produced via the luciferase enzyme (nucleotide incorporation releases pyrophosphate which is converted to ATP by ATP sulfurylase and consumed by luciferase producing light). However, the signal strength for homopolymer stretches is linear only up ...
... Measure incorporation of a nucleotide using the light produced via the luciferase enzyme (nucleotide incorporation releases pyrophosphate which is converted to ATP by ATP sulfurylase and consumed by luciferase producing light). However, the signal strength for homopolymer stretches is linear only up ...
Bonus, a Drosophila TIF1 homologue, is a chromatin
... shown to interact biochemically with several Drosophila nuclear receptors. Specifically it was demonstrated, both genetically and biochemically, that Bon is able to bind to and inhibit the transcriptional activity of βFTZ-F1, a component of the ecdysone transcriptional cascade, providing the first ...
... shown to interact biochemically with several Drosophila nuclear receptors. Specifically it was demonstrated, both genetically and biochemically, that Bon is able to bind to and inhibit the transcriptional activity of βFTZ-F1, a component of the ecdysone transcriptional cascade, providing the first ...
Gene expression profiling
In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.