Isolation and Characterization of Mutations in the b-Tubulin Gene of Saccharomyces cerevisiae .
... 3). pJT71 cut only once with Sal1 was used as a positive control, because none of the TUB2 sequences were deleted (deletion DNA no. 2). Yeast transformations for these experiments were done with the mixture of fragments generated by restriction digest. Thus, the wild-type TUB2 information is always ...
... 3). pJT71 cut only once with Sal1 was used as a positive control, because none of the TUB2 sequences were deleted (deletion DNA no. 2). Yeast transformations for these experiments were done with the mixture of fragments generated by restriction digest. Thus, the wild-type TUB2 information is always ...
predominant expression of at cell receptor v,6 gene subfamily
... PL/J clones, demonstrating that each uses the same TCR Vo gene, V" 8.2. Two distinct V08.2-J#2 combinations were identified with two clones showing one type of rearrangement and the other two sharing a separate Vs8.2Js2 combination. These results represent the first molecular analysis of the TCR of ...
... PL/J clones, demonstrating that each uses the same TCR Vo gene, V" 8.2. Two distinct V08.2-J#2 combinations were identified with two clones showing one type of rearrangement and the other two sharing a separate Vs8.2Js2 combination. These results represent the first molecular analysis of the TCR of ...
Here - Personal Genome Project Study Guide
... that occur in our bodies as we develop are often due to changes in gene activities that are regulated by our stage of development. This is known as developmental gene regulation. Different sets of genes are turned on and off as we develop and age. For the most part, the genes themselves remain const ...
... that occur in our bodies as we develop are often due to changes in gene activities that are regulated by our stage of development. This is known as developmental gene regulation. Different sets of genes are turned on and off as we develop and age. For the most part, the genes themselves remain const ...
Towards an accurate identification of mosaic genes and partial
... transgenic DNA into human cell, antibiotic-resistant genes spreading to pathogenic bacteria, and disease-associated genes spreading and recombining to create new viruses and bacteria (4). Two models of HGT have been considered in the literature (5). First, and the most popular of them, is the tradit ...
... transgenic DNA into human cell, antibiotic-resistant genes spreading to pathogenic bacteria, and disease-associated genes spreading and recombining to create new viruses and bacteria (4). Two models of HGT have been considered in the literature (5). First, and the most popular of them, is the tradit ...
Symposium Poster - uospur
... I tested this hypothesis in a fly model using specified transcripts of proteins involved in neuronal function including stnB, shab, syt1, and Unc-13 . Results indicate that loss of CG7706 has no effect on editing for Unc-13 and shab , however syt1 and stnB data does suggest that CG7706 may play some ...
... I tested this hypothesis in a fly model using specified transcripts of proteins involved in neuronal function including stnB, shab, syt1, and Unc-13 . Results indicate that loss of CG7706 has no effect on editing for Unc-13 and shab , however syt1 and stnB data does suggest that CG7706 may play some ...
Wnt8 Is Required for Growth-Zone Establishment and Development
... The posterior truncation phenotypes resulting from pRNAi against Wnt8 in the spider are at least superficially similar to those observed when Wnt8 and/or Wnt3 are perturbed in vertebrate embryos. Removal or blocking Wnt8 and/or Wnt3 in Xenopus, zebrafish, and mouse results in truncated embryos with ...
... The posterior truncation phenotypes resulting from pRNAi against Wnt8 in the spider are at least superficially similar to those observed when Wnt8 and/or Wnt3 are perturbed in vertebrate embryos. Removal or blocking Wnt8 and/or Wnt3 in Xenopus, zebrafish, and mouse results in truncated embryos with ...
Regulation of cell fusion in C. elegans - Development
... fusion is regulated by two genes of the C. elegans Hox gene cluster: lin-39, a homolog of Drosophila Sex combs reduced, and mab-5, a homolog of Drosophila Antennapedia. lin-39 is expressed in P(3-8).p in both sexes; mab-5 is also expressed in both sexes, although more posteriorly, in P(7-11).p (Sals ...
... fusion is regulated by two genes of the C. elegans Hox gene cluster: lin-39, a homolog of Drosophila Sex combs reduced, and mab-5, a homolog of Drosophila Antennapedia. lin-39 is expressed in P(3-8).p in both sexes; mab-5 is also expressed in both sexes, although more posteriorly, in P(7-11).p (Sals ...
molecular phylogeny of the haplosporidia based on
... optimal trees on the combined SSU rDNA and actin amino acid sequences because jackknife values from this data combination were intermediate among the 6 combinations tried. This search used 30 random taxon addition search sequences followed by branch breaking (TBR branch swapping). With respect to th ...
... optimal trees on the combined SSU rDNA and actin amino acid sequences because jackknife values from this data combination were intermediate among the 6 combinations tried. This search used 30 random taxon addition search sequences followed by branch breaking (TBR branch swapping). With respect to th ...
Genetics and genomics of infectious disease susceptibility
... provide compelling evidence of linkage. Chromosomal regions identified in this way are initially very large, and contain some hundreds of genes distributed over several megabases, so that much further work is usually required to actually identify the causative gene. However, the attraction of this r ...
... provide compelling evidence of linkage. Chromosomal regions identified in this way are initially very large, and contain some hundreds of genes distributed over several megabases, so that much further work is usually required to actually identify the causative gene. However, the attraction of this r ...
Speciation through evolution of sex-linked genes
... much progress has been made regarding our knowledge of how reproductive isolation evolves. This focus on the evolution of reproductive isolation probably has its basis in the biological species concept, which defines species as groups of organisms that are reproductively isolated from other such gro ...
... much progress has been made regarding our knowledge of how reproductive isolation evolves. This focus on the evolution of reproductive isolation probably has its basis in the biological species concept, which defines species as groups of organisms that are reproductively isolated from other such gro ...
Chromosome Band 1p36 Contains a Putative Tumor
... LOH was observed at D1S228, whereas heterozygosity was retained at D1S243 and D1S507 (CML no. 24). LOH was detected at D1S2667, whereas heterozygosity was retained at D1S508 and D1S199 (Fig 1C, CML no. 1). No instability was observed in this study. Two of the 14 cases showed LOH at all informative l ...
... LOH was observed at D1S228, whereas heterozygosity was retained at D1S243 and D1S507 (CML no. 24). LOH was detected at D1S2667, whereas heterozygosity was retained at D1S508 and D1S199 (Fig 1C, CML no. 1). No instability was observed in this study. Two of the 14 cases showed LOH at all informative l ...
Molecualr Biology and Evolution
... G+C gram-positives), Normand et al. ( 1992) recanted Synthetic oligonucleotides were used as primers as nectheir earlier position and suggested, in accord with essary to generate a complete sequence of n$K. Both Young ( 1992), that vertical descent is the most likely strands were completely sequence ...
... G+C gram-positives), Normand et al. ( 1992) recanted Synthetic oligonucleotides were used as primers as nectheir earlier position and suggested, in accord with essary to generate a complete sequence of n$K. Both Young ( 1992), that vertical descent is the most likely strands were completely sequence ...
Genetic Causes of Phenotypic Adaptation to the Second
... The harsh physiological conditions found in these industrial processes (low pH, high ethanol content, extreme temperature, low nitrogen availability) promote the efficacy of natural selection (Goddard, Godfray, and Burt 2005; Clifford Zeyl 2006), creating favourable conditions for the emergence of p ...
... The harsh physiological conditions found in these industrial processes (low pH, high ethanol content, extreme temperature, low nitrogen availability) promote the efficacy of natural selection (Goddard, Godfray, and Burt 2005; Clifford Zeyl 2006), creating favourable conditions for the emergence of p ...
MHC2TA Single Nucleotide Polymorphism and Genetic Risk for
... and Alberto Falorni, on behalf of the Italian Addison Network Department of Clinical Pathology and Cytology (M.G.), Karolinska University Hospital, SE-141 86 Stockholm, Sweden; Department of Internal Medicine (G.G., C.T., A.B., A.F.), Section of Internal Medicine and Endocrine and Metabolic Sciences ...
... and Alberto Falorni, on behalf of the Italian Addison Network Department of Clinical Pathology and Cytology (M.G.), Karolinska University Hospital, SE-141 86 Stockholm, Sweden; Department of Internal Medicine (G.G., C.T., A.B., A.F.), Section of Internal Medicine and Endocrine and Metabolic Sciences ...
developing corn hybrids with new traits
... years. Corn breeders are interested in how much of this increase is due to genetics and how much can be attributed to other factors. Recent studies have shown that at least 50 percent or more of the increase in corn yields since the introduction of hybrids is due to genetics (Russell, 1993). Develop ...
... years. Corn breeders are interested in how much of this increase is due to genetics and how much can be attributed to other factors. Recent studies have shown that at least 50 percent or more of the increase in corn yields since the introduction of hybrids is due to genetics (Russell, 1993). Develop ...
Plant roots: new challenges in a changing world
... maize root, the embryonic primary and seminal roots, and the post-embryonic crown roots. Although gene expression differences were relatively small (in the order of 1% of genes differentially expressed between root types), global expression profiling suggests that those genes that were different bet ...
... maize root, the embryonic primary and seminal roots, and the post-embryonic crown roots. Although gene expression differences were relatively small (in the order of 1% of genes differentially expressed between root types), global expression profiling suggests that those genes that were different bet ...
10p proximal deletions from 10p11 and 10p12
... Each new version of the genome is often referred to as an ‘assembly’ or a ‘build’. Every few years a new assembly is released. The genetic information in this guide is based on the Genome Reference Consortium (GRC) human (h) genome assembly number 37 (GRCh37), which was released in 2009. Confusingly ...
... Each new version of the genome is often referred to as an ‘assembly’ or a ‘build’. Every few years a new assembly is released. The genetic information in this guide is based on the Genome Reference Consortium (GRC) human (h) genome assembly number 37 (GRCh37), which was released in 2009. Confusingly ...
1 Are the Eyes Homologous?
... position). Clearly the observation of the similarity depends strongly on the alignment (already an important hypothesis that privileges the idea that shared residues are due to homology). It should be clear that understanding what percent of the identities are due to homology, chance, and convergenc ...
... position). Clearly the observation of the similarity depends strongly on the alignment (already an important hypothesis that privileges the idea that shared residues are due to homology). It should be clear that understanding what percent of the identities are due to homology, chance, and convergenc ...
Molecular approaches for bacterial azoreductases
... expression vector in the correct reading frame. To create site-directed mutagenesis, the coding sequence can be modified by PCR. An overexpressed construct is performed by ligation and then transformation into E. coli host strain. The transformants are screened on plates with appropriate antibiotic( ...
... expression vector in the correct reading frame. To create site-directed mutagenesis, the coding sequence can be modified by PCR. An overexpressed construct is performed by ligation and then transformation into E. coli host strain. The transformants are screened on plates with appropriate antibiotic( ...
Conservation of Brachyury (T) genes in amphioxus and vertebrates
... sequences for two reasons. First, both deduced proteins include the N-terminal limit of the conserved T-domain (see below); indeed, the AmBra-1 and mouse Brachyury proteins share three identical amino acids downstream of the deduced translation start site (Fig. 1). Second, AmBra-2 has a perfect matc ...
... sequences for two reasons. First, both deduced proteins include the N-terminal limit of the conserved T-domain (see below); indeed, the AmBra-1 and mouse Brachyury proteins share three identical amino acids downstream of the deduced translation start site (Fig. 1). Second, AmBra-2 has a perfect matc ...
Estimating the ``Effective Number of Codons`
... ^ caa -values. Alwas based on addition of all individual N though this estimator would converge toward the ‘‘true’’ Nc value with increasing gene lengths, the problem of missing amino acids was not solved, as Banerjee et al. (2005) pointed out. They therefore suggested an estimator that combined Wri ...
... ^ caa -values. Alwas based on addition of all individual N though this estimator would converge toward the ‘‘true’’ Nc value with increasing gene lengths, the problem of missing amino acids was not solved, as Banerjee et al. (2005) pointed out. They therefore suggested an estimator that combined Wri ...
Recombination - Transformation
... Two models with a single-stranded nick, gap formation and repair were proposed on the basis of cytological studies (Holliday model and Meselson-Radding model). The double-strand break and repair model was proposed on the basis of molecular studies in yeast. The model has been established in yeast bu ...
... Two models with a single-stranded nick, gap formation and repair were proposed on the basis of cytological studies (Holliday model and Meselson-Radding model). The double-strand break and repair model was proposed on the basis of molecular studies in yeast. The model has been established in yeast bu ...
Gene expression profiling
In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.