The appearance of secondary cell walls was a - Plant-o
... only produced a few dozen mutants. As the easily extractable Arabidopsis seed coat mucilage is nearly entirely composed of pectins, it provides an ideal system to study pectin synthesis by screening mutant populations. Thus we screened an Arabidopsis EMS-mutagenized population of ca. 1700 lines for ...
... only produced a few dozen mutants. As the easily extractable Arabidopsis seed coat mucilage is nearly entirely composed of pectins, it provides an ideal system to study pectin synthesis by screening mutant populations. Thus we screened an Arabidopsis EMS-mutagenized population of ca. 1700 lines for ...
Basic Principles of Transcription and Translation
... Ribozymes are catalytic RNA molecules that function as enzymes and can splice RNA The discovery of ribozymes rendered obsolete the belief that all biological catalysts were proteins Three properties of RNA enable it to function as an enzyme It can form a three-dimensional structure because o ...
... Ribozymes are catalytic RNA molecules that function as enzymes and can splice RNA The discovery of ribozymes rendered obsolete the belief that all biological catalysts were proteins Three properties of RNA enable it to function as an enzyme It can form a three-dimensional structure because o ...
S-Phase Checkpoint Genes Safeguard High
... essential (present) tag distributions: the CPM value for UPTAGs and PM value for DOWNTAGs were used. Four pairwise ratios were generated (URA3A:CTF4A, URA3A:CTF4B, URA3B:CTF4A, and URA3B:CTF4B). These were averaged to provide a single UPTAG and single DOWNTAG ratio for each knockout. Oligonucleotide ...
... essential (present) tag distributions: the CPM value for UPTAGs and PM value for DOWNTAGs were used. Four pairwise ratios were generated (URA3A:CTF4A, URA3A:CTF4B, URA3B:CTF4A, and URA3B:CTF4B). These were averaged to provide a single UPTAG and single DOWNTAG ratio for each knockout. Oligonucleotide ...
Constitutive expression of RyhB regulates the heme biosynthesis
... the carbon skeleton. This pathway is mainly present in higher plants, algae, and many bacteria. In our previous study, we engineered an Escherichia coli strain DALA that can accumulate ALA using glucose as sole carbon source through the C5 pathway (Kang et al., 2011). However, further regulation of ...
... the carbon skeleton. This pathway is mainly present in higher plants, algae, and many bacteria. In our previous study, we engineered an Escherichia coli strain DALA that can accumulate ALA using glucose as sole carbon source through the C5 pathway (Kang et al., 2011). However, further regulation of ...
Investigation 1: Examining RNA-Seq data
... mRNA that has been fully spliced is copied back to DNA with the enzyme called reverse transcriptase. Short fragments of the copied or complimentary DNA are sequenced, and then these segments are mapped back to the genome. By analyzing the mapping data, it is possible to know which and how many messe ...
... mRNA that has been fully spliced is copied back to DNA with the enzyme called reverse transcriptase. Short fragments of the copied or complimentary DNA are sequenced, and then these segments are mapped back to the genome. By analyzing the mapping data, it is possible to know which and how many messe ...
Microsoft Word - Mapping-Traits-in-Dogs
... How do you determine whether these correlations are real? It is possible that the frequency of alleles would differ between the two groups of dogs just by chance. On the other hand, if these differences did not occur by chance, the correlations might indicate that the SNPs mark the positions of gene ...
... How do you determine whether these correlations are real? It is possible that the frequency of alleles would differ between the two groups of dogs just by chance. On the other hand, if these differences did not occur by chance, the correlations might indicate that the SNPs mark the positions of gene ...
Xylitol production using recombinant Saccharomyces
... pIDXR containing an URA3 gene and then transformants were selected using the URA3 gene as an auxotrophic marker on SDC plates. Ura + transformants were randomly picked up and examined for the specific XR activity. The second transformation using the URA3 selection marker further increase the specifi ...
... pIDXR containing an URA3 gene and then transformants were selected using the URA3 gene as an auxotrophic marker on SDC plates. Ura + transformants were randomly picked up and examined for the specific XR activity. The second transformation using the URA3 selection marker further increase the specifi ...
Dihybrid Problems - Milan Area Schools
... In hogs, a gene that produces a white belt around the animal’s body is dominant over its allele for a uniformly colored body. Another gene produces a fusion of the two hoofs on each foot, a condition known as syndactyly; this gene is dominant over its allele which produces normal hoofs. Suppose a un ...
... In hogs, a gene that produces a white belt around the animal’s body is dominant over its allele for a uniformly colored body. Another gene produces a fusion of the two hoofs on each foot, a condition known as syndactyly; this gene is dominant over its allele which produces normal hoofs. Suppose a un ...
Arabidopsis brassinosteroid biosynthetic mutantdwarf7
... their amino acid sequences, the two transcription factors regulate their target genes differently; BES1 is involved in transcriptional activation [40], and BZR1 both activates and represses transcription [39,42]. As such, constitutive BR phenotypes are seen in the bes1-D mutant [40], whereas the sem ...
... their amino acid sequences, the two transcription factors regulate their target genes differently; BES1 is involved in transcriptional activation [40], and BZR1 both activates and represses transcription [39,42]. As such, constitutive BR phenotypes are seen in the bes1-D mutant [40], whereas the sem ...
Daisy quorum drives for the genetic restoration of wild
... pursuing these ideas, we would be more than happy to advise, collaborate, or desist in our own efforts as appropriate; we have no desire to duplicate the work of others given the many other urgent projects available. Even if the work is never published in a peer-reviewed journal, the data will remai ...
... pursuing these ideas, we would be more than happy to advise, collaborate, or desist in our own efforts as appropriate; we have no desire to duplicate the work of others given the many other urgent projects available. Even if the work is never published in a peer-reviewed journal, the data will remai ...
Fungal - CBS-KNAW Fungal Biodiversity Centre
... causing leaf spot on celery, sugar beet or maize, although there was an unconWrmed report of a teleomorph for C. zeae-maydis (Latterell and Rossi, 1977). Wang et al. (1998) were unable to Wnd evidence of the MAT-2 idiomorph in isolates of C. zeae-maydis, and in vitro pairing studies with isolates of ...
... causing leaf spot on celery, sugar beet or maize, although there was an unconWrmed report of a teleomorph for C. zeae-maydis (Latterell and Rossi, 1977). Wang et al. (1998) were unable to Wnd evidence of the MAT-2 idiomorph in isolates of C. zeae-maydis, and in vitro pairing studies with isolates of ...
LAB 1: Scientific Method/Tools of Scientific Inquiry
... Chickens don’t give birth to lizards and apple trees don’t give rise to pine trees. So what is the biological basis for this obvious reality? You probably already know this has to do with genes, genes one inherits from one’s parents. However the process of passing on genes from one generation to the ...
... Chickens don’t give birth to lizards and apple trees don’t give rise to pine trees. So what is the biological basis for this obvious reality? You probably already know this has to do with genes, genes one inherits from one’s parents. However the process of passing on genes from one generation to the ...
Multiple Mechanisms Contribute to Lateral Transfer of an
... +C) was calculated per each window of equal length split over the total sequence of pPDL2. The GC skew was multiplied by w/c to find its dependence on subsequence length, where w and c are lengths of the subsequence and total sequence of pPDL2, respectively. As maximum and minimum value of GC skew is ...
... +C) was calculated per each window of equal length split over the total sequence of pPDL2. The GC skew was multiplied by w/c to find its dependence on subsequence length, where w and c are lengths of the subsequence and total sequence of pPDL2, respectively. As maximum and minimum value of GC skew is ...
Student - Integrated Biology and Skills for Success in Science (IB3S)
... A Punnett square is not the only way to figure out the probabilities of the different possible offspring of a mating. Multiplying the probabilities of each gamete can work too. (in word problems the word “and” can represent multiplication, “or” usually indicates addition) For example, in the mating ...
... A Punnett square is not the only way to figure out the probabilities of the different possible offspring of a mating. Multiplying the probabilities of each gamete can work too. (in word problems the word “and” can represent multiplication, “or” usually indicates addition) For example, in the mating ...
parts
... close to the predicted results must the data be for you to be confident that they support the hypothesis? A coin toss is a good way to make and test predictions. You and a partner will each toss a coin. Then you will record the results to show whether or not either of the two coins has turned up hea ...
... close to the predicted results must the data be for you to be confident that they support the hypothesis? A coin toss is a good way to make and test predictions. You and a partner will each toss a coin. Then you will record the results to show whether or not either of the two coins has turned up hea ...
Obesity — a genetic disease of adipose tissue?
... Although the rapid increase in the prevalence of obesity in many countries suggests that environmental factors (mainly overeating and physical inactivity) play the most important role in the development of overweight, it is very likely that genetic factors also contribute. It appears that one major ...
... Although the rapid increase in the prevalence of obesity in many countries suggests that environmental factors (mainly overeating and physical inactivity) play the most important role in the development of overweight, it is very likely that genetic factors also contribute. It appears that one major ...
all chromosomes recombine in meiosis F2 plants
... Identify a BAC clone that must include your gene • Find two flanking markers contained in a single BAC (large insert plasmid) clone. • Look at GenBank entry for that BAC clone to identify candidate genes between your flanking markers – Open reading frames, – mRNA (cDNA) clone already identified, – ...
... Identify a BAC clone that must include your gene • Find two flanking markers contained in a single BAC (large insert plasmid) clone. • Look at GenBank entry for that BAC clone to identify candidate genes between your flanking markers – Open reading frames, – mRNA (cDNA) clone already identified, – ...
Functions of the nonsense-mediated RNA decay pathway in Drosophila development. PLoS Genetics 2 , 2143-2154.
... production of truncated, potentially harmful proteins that can interfere with normal cellular processes (e.g., [3]). The NMD pathway has also been found to influence expression of a variety of wild-type transcripts (reviewed in [4]), implying that the pathway has regulatory roles beyond its surveill ...
... production of truncated, potentially harmful proteins that can interfere with normal cellular processes (e.g., [3]). The NMD pathway has also been found to influence expression of a variety of wild-type transcripts (reviewed in [4]), implying that the pathway has regulatory roles beyond its surveill ...
Nucleic Acids Research, 32: D489-D492 (2004).
... that Alu subfamilies have originated through successive waves of ®xation from sequential small subsets of active Alu sequences. The oldest Alu-related elements are the monomeric FAM, FRAM and FLAM sequences. The oldest Alu dimeric subfamilies are Alu-Jo and Alu-Jb, estimated to be ~80 million years ...
... that Alu subfamilies have originated through successive waves of ®xation from sequential small subsets of active Alu sequences. The oldest Alu-related elements are the monomeric FAM, FRAM and FLAM sequences. The oldest Alu dimeric subfamilies are Alu-Jo and Alu-Jb, estimated to be ~80 million years ...
Mutation in xyloglucan 6-xylosytransferase results in abnormal root
... root hair density or distribution pattern between srh2 and wild type (Fig. 1D, E). It is reported that extracellular pH can regulate root hair growth by modification to cell wall rigidity (Monshausen et al., 2007). To investigate whether the root hair growth of srh2 is affected by extracellular pH, ...
... root hair density or distribution pattern between srh2 and wild type (Fig. 1D, E). It is reported that extracellular pH can regulate root hair growth by modification to cell wall rigidity (Monshausen et al., 2007). To investigate whether the root hair growth of srh2 is affected by extracellular pH, ...
imbalances within regions containing large
... containing genes which may have contributed to the abnormal phenotype. ¾ Aberrations observed by either DNA microarray or subtelomeric FISH studies that have not been associated with a specific phenotype in other case studies or cannot be tied to a possible causative gene within that region may repr ...
... containing genes which may have contributed to the abnormal phenotype. ¾ Aberrations observed by either DNA microarray or subtelomeric FISH studies that have not been associated with a specific phenotype in other case studies or cannot be tied to a possible causative gene within that region may repr ...
Covrig I., Oroian I., Patrutoiu T. C., 2013 The C locus
... Two Pigments, a Lot of Colors. A rabbit has only two possible pigments that can be expressed in its fur: one is yellow and the other is dark brown. The absence of both yellow and dark brown pigments results in completely white animals (see Figs 8-9) (Searle 1990). Because of the absence of pigments ...
... Two Pigments, a Lot of Colors. A rabbit has only two possible pigments that can be expressed in its fur: one is yellow and the other is dark brown. The absence of both yellow and dark brown pigments results in completely white animals (see Figs 8-9) (Searle 1990). Because of the absence of pigments ...
CHARACTER CHANGES CAUSED BY MUTATION OF AN ENTIRE
... the region opposite to the deficient piece, similar to that which has been demonstrated in the case of the mutant genes. It is superfluous to regard the character notch as due to an independent specific mutant gene contained in or linked to the deficient region. It would seem probable that many norm ...
... the region opposite to the deficient piece, similar to that which has been demonstrated in the case of the mutant genes. It is superfluous to regard the character notch as due to an independent specific mutant gene contained in or linked to the deficient region. It would seem probable that many norm ...
Current Awareness Of Issues Related To Genetically Modified Food
... transformation of plants are no different to those seen using traditional translocation and mutation breeding and so should not be required to comply with regulations governing the growing and processing of GM crops. As all the DNA in a plant transformed using an intragenic vector system is derived ...
... transformation of plants are no different to those seen using traditional translocation and mutation breeding and so should not be required to comply with regulations governing the growing and processing of GM crops. As all the DNA in a plant transformed using an intragenic vector system is derived ...
Gene expression profiling
In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.