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Genome-Scale CRISPR-Mediated Control of the Gene
Genome-Scale CRISPR-Mediated Control of the Gene

... Ricin-resistance phenotypes, comparing CRISPRi and sgRNAs for genes previously established to cause ricinresistance phenotypes when knocked down by RNAi. ...
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This examination paper consists of 4 pages

... (numbers in brackets indicate the number of correct answers) 1. Which of the following statements are true Transcriptomes consist of functional RNAs Genes can be disrupted by RNA interference The genetic code includes 1 termination codon 3’ ends of genes can be identified by rapid amplification of c ...
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... • DNA  must  be  read  from  5’  to  3’  end   • RNA  usually  single-­‐stranded   o Uracil  replaces  Thymine  in  RNA   • Gene  transcription:  Transcription  factors  recognize  binding  sites  in  DNA,   recruits  RNA  polymerase   o RNA ...
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... Genes from other organisms can work together and still function (ex. luciferase and tobacco plants)  Transgenic—contain genes from other organisms  A gene from one organism can be inserted into cells from another organism.  Genetic engineering has spurred the growth of biotechnology, a new indust ...
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Gene



A gene is a locus (or region) of DNA that encodes a functional RNA or protein product, and is the molecular unit of heredity. The transmission of genes to an organism's offspring is the basis of the inheritance of phenotypic traits. Most biological traits are under the influence of polygenes (many different genes) as well as the gene–environment interactions. Some genetic traits are instantly visible, such as eye colour or number of limbs, and some are not, such as blood type, risk for specific diseases, or the thousands of basic biochemical processes that comprise life.Genes can acquire mutations in their sequence, leading to different variants, known as alleles, in the population. These alleles encode slightly different versions of a protein, which cause different phenotype traits. Colloquial usage of the term ""having a gene"" (e.g., ""good genes,"" ""hair colour gene"") typically refers to having a different allele of the gene. Genes evolve due to natural selection or survival of the fittest of the alleles.The concept of a gene continues to be refined as new phenomena are discovered. For example, regulatory regions of a gene can be far removed from its coding regions, and coding regions can be split into several exons. Some viruses store their genome in RNA instead of DNA and some gene products are functional non-coding RNAs. Therefore, a broad, modern working definition of a gene is any discrete locus of heritable, genomic sequence which affect an organism's traits by being expressed as a functional product or by regulation of gene expression.
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