Getting Started
... which tools have data for your species of interest? what type of accessions are accepted? availability (commercial and freely available) ...
... which tools have data for your species of interest? what type of accessions are accepted? availability (commercial and freely available) ...
splicing.pdf
... One of the ways in which the vertebrate genome is more complex than that of other organisms is by increased use of alternative splicing. In alternative splicing, more than one protein product is made from one gene. This explains how vertebrates are able to make 5 times as many proteins as flies or w ...
... One of the ways in which the vertebrate genome is more complex than that of other organisms is by increased use of alternative splicing. In alternative splicing, more than one protein product is made from one gene. This explains how vertebrates are able to make 5 times as many proteins as flies or w ...
Predicting_tRNA_and_tmRNA_genes_12-2-16
... The phages that contain more than 1 tRNA within their genomes tend to localize the tRNAs to certain regions of the genomes (also called “tRNA clusters” in the phage tRNA literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the other ...
... The phages that contain more than 1 tRNA within their genomes tend to localize the tRNAs to certain regions of the genomes (also called “tRNA clusters” in the phage tRNA literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the other ...
Predicting tRNA and tmRNA genes Aragorn - SEA
... The phages that contain more than 1 tRNA within their genomes tend to localize the tRNAs to certain regions of the genomes (also called “tRNA clusters” in the phage tRNA literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the other ...
... The phages that contain more than 1 tRNA within their genomes tend to localize the tRNAs to certain regions of the genomes (also called “tRNA clusters” in the phage tRNA literature.) It is highly unusual that a phage with multiple tRNAs will contain a sole tRNA distant genomically from all the other ...
Doc-Help - MB DNA Analysis
... make a special section in the report with a list of the enzymes which have their cutting sequences somewhere in the DNA, but NOT in the specified region. 7. Check the “Plot GC percentage on the map” to be able to see the GC content of every 10 bp block for the sequences under 7000 bp. If the sequenc ...
... make a special section in the report with a list of the enzymes which have their cutting sequences somewhere in the DNA, but NOT in the specified region. 7. Check the “Plot GC percentage on the map” to be able to see the GC content of every 10 bp block for the sequences under 7000 bp. If the sequenc ...
Bacterial physiological adaptations to contrasting edaphic
... were found to reflect survival and nutrient acquisition strategies, with organic-rich acidic soils harboring a greater abundance of cation efflux pumps, C and N direct fixation systems and fermentation pathways indicative of anaerobiosis. Conversely, high pH soils possessed more direct transporter-m ...
... were found to reflect survival and nutrient acquisition strategies, with organic-rich acidic soils harboring a greater abundance of cation efflux pumps, C and N direct fixation systems and fermentation pathways indicative of anaerobiosis. Conversely, high pH soils possessed more direct transporter-m ...
Gene Section PDGFRA (platelet-derived growth factor receptor, alpha polypeptide)
... growth factor receptor alpha (PDGFRA) kinase in patients with gastrointestinal stromal tumors. Most common mutations are in exon 18, such as the D842V substitution that shows resistance to imatinib. Mutations in the juxtamembrane domain (exon 12; V561D most common) and in exon 14 tyrosine kinase 1 ( ...
... growth factor receptor alpha (PDGFRA) kinase in patients with gastrointestinal stromal tumors. Most common mutations are in exon 18, such as the D842V substitution that shows resistance to imatinib. Mutations in the juxtamembrane domain (exon 12; V561D most common) and in exon 14 tyrosine kinase 1 ( ...
Co-dominant SCAR marker for detection of the begomovirus
... different from those for both M82-1-8 and H24. Most notably, the 120-nt indel associated with the fragments from M82-1-8 and H24 was not present between the M82-1-8 and LA2779 sequences for T0302. These results support the presence of an introgression from S. habrochaites in H24 at this marker. This ...
... different from those for both M82-1-8 and H24. Most notably, the 120-nt indel associated with the fragments from M82-1-8 and H24 was not present between the M82-1-8 and LA2779 sequences for T0302. These results support the presence of an introgression from S. habrochaites in H24 at this marker. This ...
5 Genetic Analysis of Kidney Disease in Mice
... genes in these small regions were tested using two expression databases to determine if the genes were expressed in the tissues relevant to the phenotype. Those genes meeting this criterion were then examined for coding sequence differences using first a database and then actual sequencing. These ge ...
... genes in these small regions were tested using two expression databases to determine if the genes were expressed in the tissues relevant to the phenotype. Those genes meeting this criterion were then examined for coding sequence differences using first a database and then actual sequencing. These ge ...
Gene list - Bioinformatics.ca
... if only portion of the total gene complement is queried (or available for annotation), only use that population as background. • To test for enrichment of more than one independent types of annotation (red vs black and circle vs square), apply Fisher’s exact test separately for each type. ***More on ...
... if only portion of the total gene complement is queried (or available for annotation), only use that population as background. • To test for enrichment of more than one independent types of annotation (red vs black and circle vs square), apply Fisher’s exact test separately for each type. ***More on ...
Phenotypic plasticity can potentiate rapid evolutionary change
... draw attention from evolutionary and developmental biologists (see Gilbert, 2000). A typical experiment, sketched here to illustrate the principle rather than describe details, goes as follows. To begin with, independent lines of brother–sister matings are set up between true-breeding wild-type flies ...
... draw attention from evolutionary and developmental biologists (see Gilbert, 2000). A typical experiment, sketched here to illustrate the principle rather than describe details, goes as follows. To begin with, independent lines of brother–sister matings are set up between true-breeding wild-type flies ...
Note: Alleles are alternative forms of a, gene which occupies a
... 8 In humans, maleness or femaleness is determined by a pair of sex chromosomes called X and Y. (a) What is the genotype for males? XY (1) (b) What is the genotype for females? XX (1) 9 (a) In humans, is it the sperm or the ovum which determines the sex of the offspring? Sperm (1) (b) Give a reason f ...
... 8 In humans, maleness or femaleness is determined by a pair of sex chromosomes called X and Y. (a) What is the genotype for males? XY (1) (b) What is the genotype for females? XX (1) 9 (a) In humans, is it the sperm or the ovum which determines the sex of the offspring? Sperm (1) (b) Give a reason f ...
Woods Hole – Zebrafish Genetics and Development Bioinformatics
... pattern consistent with the in situ data: expression in some tissues is absent, and many tissues express GFP where the gene is not normally expressed. How might you explain these results? You decide to make a new reporter line by BAC recombination: you will obtain a large (~200kb) chunk of genomic D ...
... pattern consistent with the in situ data: expression in some tissues is absent, and many tissues express GFP where the gene is not normally expressed. How might you explain these results? You decide to make a new reporter line by BAC recombination: you will obtain a large (~200kb) chunk of genomic D ...
DNA Specificity of the Bicoid Activator Protein Is Determined by
... at position 1 and still activated a Bicoid site target. LexABicoid mutant A5 contained a Lys+Ala substitution at position 5 and also activated a Bicoid site target but at reduced levels. However, activation by this mutant was also reduced using a LexA site target, perhaps because the protein is less ...
... at position 1 and still activated a Bicoid site target. LexABicoid mutant A5 contained a Lys+Ala substitution at position 5 and also activated a Bicoid site target but at reduced levels. However, activation by this mutant was also reduced using a LexA site target, perhaps because the protein is less ...
Drought response in the spikes of barley: gene expression in the
... Differential gene expression among drought-stressed spike organs was analyzed using a mixed linear model analysis (Nettleton 2006). The log2 expression values were the response variables, drought stress and organ type were fixed treatment effects, and date of sample collection (block) and residuals ...
... Differential gene expression among drought-stressed spike organs was analyzed using a mixed linear model analysis (Nettleton 2006). The log2 expression values were the response variables, drought stress and organ type were fixed treatment effects, and date of sample collection (block) and residuals ...
Extrapolation to the whole human genome
... pseudogenes (Figure 3). There is little similarity between all three lists (genes, processed pseudogenes and non-processed pseudogenes for chromosomes 21 and 22 combined); the ‘transcription factor’ functional classes occurs in the top five of all three (processed pseudogenes, non-processed pseudoge ...
... pseudogenes (Figure 3). There is little similarity between all three lists (genes, processed pseudogenes and non-processed pseudogenes for chromosomes 21 and 22 combined); the ‘transcription factor’ functional classes occurs in the top five of all three (processed pseudogenes, non-processed pseudoge ...
Use of Gene-Specific Primer Cocktails for First
... synthesis. The 5′ or 3′ bias is up to the experiSensitivity of cocktail-primed RT-PCR. a) Cocktail RT product was used in this study flank the intron; therefore, α1AR Figure 2 menter because the primer can be designed to anneal anywhere in the transcript. message is the only possible template for PC ...
... synthesis. The 5′ or 3′ bias is up to the experiSensitivity of cocktail-primed RT-PCR. a) Cocktail RT product was used in this study flank the intron; therefore, α1AR Figure 2 menter because the primer can be designed to anneal anywhere in the transcript. message is the only possible template for PC ...
The Arabidopsis NAC Transcription Factor VNI2
... Among the NAC transcription factor genes identified in Arabidopsis, VNI2 (AT5G13180) is of particular interest in that it is influenced by both developmental and environmental cues. Gene expression analysis using the GENEVESTIGATOR database (https://www.genevestigator.com/gv/index.jsp) revealed that ...
... Among the NAC transcription factor genes identified in Arabidopsis, VNI2 (AT5G13180) is of particular interest in that it is influenced by both developmental and environmental cues. Gene expression analysis using the GENEVESTIGATOR database (https://www.genevestigator.com/gv/index.jsp) revealed that ...
Meristematic sculpting in fruit development
... Introduction To understand the end, we must return to the beginning and, for most plants, the beginning is the meristem. The meristem is a tissue in which cells are actively dividing and giving rise to relatively undifferentiated cells (Oxford English Dictionary, Online edition http://dictionary.oed ...
... Introduction To understand the end, we must return to the beginning and, for most plants, the beginning is the meristem. The meristem is a tissue in which cells are actively dividing and giving rise to relatively undifferentiated cells (Oxford English Dictionary, Online edition http://dictionary.oed ...
Gene Expression
... Pairing of complementary bases is the key to the transfer of information from DNA to RNA and from RNA to protein Polarities of DNA, RNA, and polypeptides help guide the mechanisms of gene expression Gene expression requires input of energy and participation of specific proteins and macromolecular as ...
... Pairing of complementary bases is the key to the transfer of information from DNA to RNA and from RNA to protein Polarities of DNA, RNA, and polypeptides help guide the mechanisms of gene expression Gene expression requires input of energy and participation of specific proteins and macromolecular as ...
Promega Notes: Technically Speaking: Tips for Working with RNA
... phenol:chloroform:isoamyl alcohol, adequate extraction reagents for the amount and type of tissue used and proper pH for the extraction reagents. Commercial reagents are preferred because of the difficulty in making adequately pure and consistent reagents at the exact pH necessary to partition DNA ...
... phenol:chloroform:isoamyl alcohol, adequate extraction reagents for the amount and type of tissue used and proper pH for the extraction reagents. Commercial reagents are preferred because of the difficulty in making adequately pure and consistent reagents at the exact pH necessary to partition DNA ...
Roles of BRCA1 and BRCA2 in homologous recombination, DNA
... Identification of the BRCA genes The genetic basis for familial breast cancer predisposition has become established over the past decade with the cloning of two major breast cancer susceptibility genes, BRCA1 and BRCA2 (Miki et al., 1994; Wooster et al., 1995). The BRCA1 gene was first cloned in 1994 ...
... Identification of the BRCA genes The genetic basis for familial breast cancer predisposition has become established over the past decade with the cloning of two major breast cancer susceptibility genes, BRCA1 and BRCA2 (Miki et al., 1994; Wooster et al., 1995). The BRCA1 gene was first cloned in 1994 ...
Depleting Gene Activities in Early Drosophila Embryos
... cannot, reflecting the early activity of Torso signaling and the later function of EGFR signaling. The D-raf example illustrates how different embryonic phenotypes can be observed depending on the level of either maternal or zygotic gene activity present at a specific developmental stage. Recently, we ...
... cannot, reflecting the early activity of Torso signaling and the later function of EGFR signaling. The D-raf example illustrates how different embryonic phenotypes can be observed depending on the level of either maternal or zygotic gene activity present at a specific developmental stage. Recently, we ...
Drosophila windpipe codes for a leucine
... Fig. 3. Embryonic expression pattern of wdp. wdp transcripts were detected in whole-mount embryos by in situ hybridization of digoxygenin-labeled anti-sense RNA derived from lgt10 clone 8.2-2. (A–G) wdp expression was first detected at early stage 15. Images (A–G) are of the same embryo. (A) Lateral ...
... Fig. 3. Embryonic expression pattern of wdp. wdp transcripts were detected in whole-mount embryos by in situ hybridization of digoxygenin-labeled anti-sense RNA derived from lgt10 clone 8.2-2. (A–G) wdp expression was first detected at early stage 15. Images (A–G) are of the same embryo. (A) Lateral ...
Gene
A gene is a locus (or region) of DNA that encodes a functional RNA or protein product, and is the molecular unit of heredity. The transmission of genes to an organism's offspring is the basis of the inheritance of phenotypic traits. Most biological traits are under the influence of polygenes (many different genes) as well as the gene–environment interactions. Some genetic traits are instantly visible, such as eye colour or number of limbs, and some are not, such as blood type, risk for specific diseases, or the thousands of basic biochemical processes that comprise life.Genes can acquire mutations in their sequence, leading to different variants, known as alleles, in the population. These alleles encode slightly different versions of a protein, which cause different phenotype traits. Colloquial usage of the term ""having a gene"" (e.g., ""good genes,"" ""hair colour gene"") typically refers to having a different allele of the gene. Genes evolve due to natural selection or survival of the fittest of the alleles.The concept of a gene continues to be refined as new phenomena are discovered. For example, regulatory regions of a gene can be far removed from its coding regions, and coding regions can be split into several exons. Some viruses store their genome in RNA instead of DNA and some gene products are functional non-coding RNAs. Therefore, a broad, modern working definition of a gene is any discrete locus of heritable, genomic sequence which affect an organism's traits by being expressed as a functional product or by regulation of gene expression.