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Chapter3 atoms
Chapter3 atoms

... Dalton’s Atomic Theory (1808)  All matter is composed of extremely small particles called atoms  Atoms of a given element are identical in size, mass, and other properties; atoms of different John Dalton elements differ in size, mass, and other properties  Atoms cannot be subdivided, created, or ...
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... properties as their parent element since neutrons do not affect how they react. Isotopes with more neutrons are heavier and move more slowly at a given temperature. This can be used as a means to separate them. ...
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... 1. amu appropriate unit for measuring the size of a particle 2. equals one-twelfth the mass of a carbon atom C. Protons 1. number of protons is used to identify elements 2. each element has different number of protons 3. number of electrons equals the number of protons in an element ...
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Isotopic labeling



Isotopic labeling (or isotopic labelling) is a technique used to track the passage of an isotope, or an atom with a variation, through a reaction, metabolic pathway, or cell. The reactant is 'labeled' by replacing specific atoms by their isotope. The reactant is then allowed to undergo the reaction. The position of the isotopes in the products is measured to determine the sequence the isotopic atom followed in the reaction or the cell's metabolic pathway. The nuclides used in isotopic labeling may be stable nuclides or radionuclides. In the latter case, the labeling is called radiolabeling.In isotopic labeling, there are multiple ways to detect the presence of labeling isotopes; through their mass, vibrational mode, or radioactive decay. Mass spectrometry detects the difference in an isotope's mass, while infrared spectroscopy detects the difference in the isotope's vibrational modes. Nuclear magnetic resonance detects atoms with different gyromagnetic ratios. The radioactive decay can be detected through an ionization chamber or autoradiographs of gels.An example of the use of isotopic labeling is the study of phenol (C6H5OH) in water by replacing common hydrogen (protium) with deuterium (deuterium labeling). Upon adding phenol to deuterated water (water containing D2O in addition to the usual H2O), the substitution of deuterium for the hydrogen is observed in phenol's hydroxyl group (resulting in C6H5OD), indicating that phenol readily undergoes hydrogen-exchange reactions with water. Only the hydroxyl group was affected, indicating that the other 5 hydrogen atoms did not participate in these exchange reactions.
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