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PowerPoint Presentation - The Atom: Chp 12 sect 2
PowerPoint Presentation - The Atom: Chp 12 sect 2

... the same, • although the physical properties of some isotopes may be different. • Some isotopes are radioactivemeaning they "radiate" energy as they decay to a more stable form, • perhaps another element half-life: time required for half of the atoms of an element to decay into stable form. ...
Atomic Structure
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... 2. Atoms of the same element are identical. Atoms of different elements differ. 3. Atoms cannot be divided, created, nor destroyed. 4. Atoms combine in simple whole number ratios to form compounds. 5. Atoms are rearranged, separated, or combined in chemical reactions. ...
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Chemistry Worksheet: Atomic Structure and Isotopes

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Isotopic labeling



Isotopic labeling (or isotopic labelling) is a technique used to track the passage of an isotope, or an atom with a variation, through a reaction, metabolic pathway, or cell. The reactant is 'labeled' by replacing specific atoms by their isotope. The reactant is then allowed to undergo the reaction. The position of the isotopes in the products is measured to determine the sequence the isotopic atom followed in the reaction or the cell's metabolic pathway. The nuclides used in isotopic labeling may be stable nuclides or radionuclides. In the latter case, the labeling is called radiolabeling.In isotopic labeling, there are multiple ways to detect the presence of labeling isotopes; through their mass, vibrational mode, or radioactive decay. Mass spectrometry detects the difference in an isotope's mass, while infrared spectroscopy detects the difference in the isotope's vibrational modes. Nuclear magnetic resonance detects atoms with different gyromagnetic ratios. The radioactive decay can be detected through an ionization chamber or autoradiographs of gels.An example of the use of isotopic labeling is the study of phenol (C6H5OH) in water by replacing common hydrogen (protium) with deuterium (deuterium labeling). Upon adding phenol to deuterated water (water containing D2O in addition to the usual H2O), the substitution of deuterium for the hydrogen is observed in phenol's hydroxyl group (resulting in C6H5OD), indicating that phenol readily undergoes hydrogen-exchange reactions with water. Only the hydroxyl group was affected, indicating that the other 5 hydrogen atoms did not participate in these exchange reactions.
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