ppt
... Cas9 functions as an RNA-guided DNA binding protein when engineered to contain inactivating mutations in both of its active sites. Dead Cas9 (dCas9) can be fused to: •activator or repressor domains for transcriptional down-regulation or activation . •fused to fluorescent domains, (eg. GFP), for liv ...
... Cas9 functions as an RNA-guided DNA binding protein when engineered to contain inactivating mutations in both of its active sites. Dead Cas9 (dCas9) can be fused to: •activator or repressor domains for transcriptional down-regulation or activation . •fused to fluorescent domains, (eg. GFP), for liv ...
Multi-copy suppressor screen
... fates, depending on the kinds of sequences present on the DNA and whether and where the DNA is linearized. In the experiment today, the DNA you are using is a circular, supercoiled DNA isolated from E. coli as a plasmid. Because these plasmids (for example pRS316) have an ARS (autonomously replicati ...
... fates, depending on the kinds of sequences present on the DNA and whether and where the DNA is linearized. In the experiment today, the DNA you are using is a circular, supercoiled DNA isolated from E. coli as a plasmid. Because these plasmids (for example pRS316) have an ARS (autonomously replicati ...
Daily Warm Ups, Q1
... • What happens during the “S” phase of Interphase? • Diploid or Haploid? Gametes Zygote ...
... • What happens during the “S” phase of Interphase? • Diploid or Haploid? Gametes Zygote ...
Polymerase Chain Reaction
... Base pair (bp): Two nitrogenous bases ( adenine and thymine or guanine and cytosine) held together by weak bonds. Two strands of DNA are held together in the shape of a double helix by the bonds between base pairs. Base sequence: The order of nucleotide bases in a DNA molecule. Centromere: A special ...
... Base pair (bp): Two nitrogenous bases ( adenine and thymine or guanine and cytosine) held together by weak bonds. Two strands of DNA are held together in the shape of a double helix by the bonds between base pairs. Base sequence: The order of nucleotide bases in a DNA molecule. Centromere: A special ...
PowerPoint Presentation - Meiosis
... http://gslc.genetics.utah.edu/units/disorders/karyotype/karyotype.cfm ...
... http://gslc.genetics.utah.edu/units/disorders/karyotype/karyotype.cfm ...
Phenotype/Genotype Phenotype/Genotype cont. The sickle cell
... will “express” (have) type A blood. An individual with EITHER 2 B alleles or 1 B and 1 O will have type B blood. An individual with one A and one B allele will have AB ...
... will “express” (have) type A blood. An individual with EITHER 2 B alleles or 1 B and 1 O will have type B blood. An individual with one A and one B allele will have AB ...
S E X and G E N E S
... Humankind has been playing with the genetic code of organisms for thousands of years. Animal husbandry: the mating of domesticated animals to produce offspring with desirable ...
... Humankind has been playing with the genetic code of organisms for thousands of years. Animal husbandry: the mating of domesticated animals to produce offspring with desirable ...
Genetics- Part 1- Genes
... Mendel was an Austrian monk who taught natural science and worked on plant breeding experiments. He developed a basic understanding of genetics and inheritance. Mendel’s Work It took him 2 years to select the pea plant as his subject. He collected data for 10 years. His sample sizes were large; he t ...
... Mendel was an Austrian monk who taught natural science and worked on plant breeding experiments. He developed a basic understanding of genetics and inheritance. Mendel’s Work It took him 2 years to select the pea plant as his subject. He collected data for 10 years. His sample sizes were large; he t ...
Learning Grid Cellular control
... same chromosome they are referred to as what? What is the significance of linkage during meiosis? Where are most sex-linked genes found? Which of these denotes that the two alleles (A/a and B/b) are linked? Circle the correct ...
... same chromosome they are referred to as what? What is the significance of linkage during meiosis? Where are most sex-linked genes found? Which of these denotes that the two alleles (A/a and B/b) are linked? Circle the correct ...
Acute diarrhea
... A chromosome is very much wider than the diameter of a DNA double helix. In addition, the amount of DNA in the nucleus of each cell in humans means that the total length of DNA contained in the chromosomes, if fully extended, would be several meters long . Under the electron microscope chromosomes c ...
... A chromosome is very much wider than the diameter of a DNA double helix. In addition, the amount of DNA in the nucleus of each cell in humans means that the total length of DNA contained in the chromosomes, if fully extended, would be several meters long . Under the electron microscope chromosomes c ...
Proteins and Mutations
... Note – Most cases of Down’s syndrome involve the person having an extra chromosome 21. This is not a translocation mutation but you may have noticed that some people with Down’s are affected to a different degree. This is called familial Down’s syndrome which is caused by translocation 5 % of Down’s ...
... Note – Most cases of Down’s syndrome involve the person having an extra chromosome 21. This is not a translocation mutation but you may have noticed that some people with Down’s are affected to a different degree. This is called familial Down’s syndrome which is caused by translocation 5 % of Down’s ...
Chapter13 Section03 cell transformation ppt
... In nature, a bacterium exists that produces tumors in plant cells. Researchers can inactivate the tumor-producing gene found in this bacterium and insert a piece of foreign DNA into the plasmid. The recombinant plasmid can then be used to ...
... In nature, a bacterium exists that produces tumors in plant cells. Researchers can inactivate the tumor-producing gene found in this bacterium and insert a piece of foreign DNA into the plasmid. The recombinant plasmid can then be used to ...
3 Cell Transformation
... In nature, a bacterium exists that produces tumors in plant cells. Researchers can inactivate the tumor-producing gene found in this bacterium and insert a piece of foreign DNA into the plasmid. The recombinant plasmid can then be used to ...
... In nature, a bacterium exists that produces tumors in plant cells. Researchers can inactivate the tumor-producing gene found in this bacterium and insert a piece of foreign DNA into the plasmid. The recombinant plasmid can then be used to ...
AP Biology 2015 - 2016 Cerveny Lab Bench Investigation: Mitosis
... Once you have accessed the site complete: Key Concepts, Design of Experiments, Analysis of Results and Lab Quiz by reading the information presented, watching the animations. For the meiosis section answer all questions in your lab notebook. The questions are in italics. ...
... Once you have accessed the site complete: Key Concepts, Design of Experiments, Analysis of Results and Lab Quiz by reading the information presented, watching the animations. For the meiosis section answer all questions in your lab notebook. The questions are in italics. ...
Human Genetics
... All humans have testosterone, but males have much higher levels of this hormone than females do. Dominance only matters in the heterozygote, so heterozygous males experience hair loss and heterozygous females don’t. Homozygous females may experience no more than a thinning of their hair, altho ...
... All humans have testosterone, but males have much higher levels of this hormone than females do. Dominance only matters in the heterozygote, so heterozygous males experience hair loss and heterozygous females don’t. Homozygous females may experience no more than a thinning of their hair, altho ...
Q1. The diagrams show four cells, A, B, C and D. Use letters A, B, C
... from hard parts / bones / imprints e.g. footprints / parts replaced by other materials / conditions for ...
... from hard parts / bones / imprints e.g. footprints / parts replaced by other materials / conditions for ...
No Slide Title
... A cell with its selectively permeable membrane is quickly affected by osmotic pressure changes. It is important for body fluid concentrations to be maintained within narrow limits to stop cells shrinking or swelling and bursting. A salt solution that develops the same osmotic pressure as normal body ...
... A cell with its selectively permeable membrane is quickly affected by osmotic pressure changes. It is important for body fluid concentrations to be maintained within narrow limits to stop cells shrinking or swelling and bursting. A salt solution that develops the same osmotic pressure as normal body ...
5. Why are there several children with Down syndrome in my family?
... that a translocation may be being inherited in this family. It would therefore be extremely important to check the chromosomes of the two people affected with Down syndrome to confirm their karyotypes. If both had trisomy 21, due to unrelated errors in meiosis in different people, other family membe ...
... that a translocation may be being inherited in this family. It would therefore be extremely important to check the chromosomes of the two people affected with Down syndrome to confirm their karyotypes. If both had trisomy 21, due to unrelated errors in meiosis in different people, other family membe ...
Unit Test: Genetics Name: Date: Period: The diagram shows a plant
... As a result, one flatworm will have produced three offspring. What conclusion can you make from these observations? The flatworm produces — A. offspring identical to one another but different from the parent B. offspring that are identical to each other and the parent C. three diverse offspring D. o ...
... As a result, one flatworm will have produced three offspring. What conclusion can you make from these observations? The flatworm produces — A. offspring identical to one another but different from the parent B. offspring that are identical to each other and the parent C. three diverse offspring D. o ...
It this a DNA or RNA virus? Is it single
... Draw the cell pedigree, showing this chromosome in the two daughter and four granddaughter cells. (For the purposes of this question, assume that replication uses a primer that is only 3 bases long. Also, real chromosomes would have specific sequences at the ends- I just used these because they are ...
... Draw the cell pedigree, showing this chromosome in the two daughter and four granddaughter cells. (For the purposes of this question, assume that replication uses a primer that is only 3 bases long. Also, real chromosomes would have specific sequences at the ends- I just used these because they are ...
Chromosome
A chromosome (chromo- + -some) is a packaged and organized structure containing most of the DNA of a living organism. It is not usually found on its own, but rather is complexed with many structural proteins called histones as well as associated transcription (copying of genetic sequences) factors and several other macromolecules. Two ""sister"" chromatids (half a chromosome) join together at a protein junction called a centromere. Chromosomes are normally visible under a light microscope only when the cell is undergoing mitosis. Even then, the full chromosome containing both joined sister chromatids becomes visible only during a sequence of mitosis known as metaphase (when chromosomes align together, attached to the mitotic spindle and prepare to divide). This DNA and its associated proteins and macromolecules is collectively known as chromatin, which is further packaged along with its associated molecules into a discrete structure called a nucleosome. Chromatin is present in most cells, with a few exceptions - erythrocytes for example. Occurring only in the nucleus of eukaryotic cells, chromatin composes the vast majority of all DNA, except for a small amount inherited maternally which is found in mitochondria. In prokaryotic cells, chromatin occurs free-floating in cytoplasm, as these cells lack organelles and a defined nucleus. The main information-carrying macromolecule is a single piece of coiled double-stranded DNA, containing many genes, regulatory elements and other noncoding DNA. The DNA-bound macromolecules are proteins, which serve to package the DNA and control its functions. Chromosomes vary widely between different organisms. Some species such as certain bacteria also contain plasmids or other extrachromosomal DNA. These are circular structures in the cytoplasm which contain cellular DNA and play a role in horizontal gene transfer.Compaction of the duplicated chromosomes during cell division (mitosis or meiosis) results either in a four-arm structure (pictured to the right) if the centromere is located in the middle of the chromosome or a two-arm structure if the centromere is located near one of the ends. Chromosomal recombination during meiosis and subsequent sexual reproduction plays a vital role in genetic diversity. If these structures are manipulated incorrectly, through processes known as chromosomal instability and translocation, the cell may undergo mitotic catastrophe and die, or it may unexpectedly evade apoptosis leading to the progression of cancer.In prokaryotes (see nucleoids) and viruses, the DNA is often densely packed and organized. In the case of archaea by homologs to eukaryotic histones, in the case of bacteria by histone-like proteins. Small circular genomes called plasmids are often found in bacteria and also in mitochondria and chloroplasts, reflecting their bacterial origins.