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... on the day of injection and fixed in Bouin's fixative, after which they were embedded in paraffin, sectioned at 6 jum and then stained with haematoxylin and eosin. These larvae were examined microscopically to ascertain the degree of maturation of the thymus and spleen. Immuno-cytoadherence assays w ...
... on the day of injection and fixed in Bouin's fixative, after which they were embedded in paraffin, sectioned at 6 jum and then stained with haematoxylin and eosin. These larvae were examined microscopically to ascertain the degree of maturation of the thymus and spleen. Immuno-cytoadherence assays w ...
In vitro effects of different innate-immune stimulants on
... In addition, we tried to acquire an antibody for Atlantic salmon cathelicidin by immunization of rabbit with synthetic peptides derived from cathelicidin. Specific cathelicidin antibodies were not obtained in this experiment. The findings might contribute to the development of alternatives to the cl ...
... In addition, we tried to acquire an antibody for Atlantic salmon cathelicidin by immunization of rabbit with synthetic peptides derived from cathelicidin. Specific cathelicidin antibodies were not obtained in this experiment. The findings might contribute to the development of alternatives to the cl ...
Identification of the yaa lupus as a IIr
... sera strongly reacted with RNA-containing antigens [9]. Thus, it appears that the Yaa locus bears gene(s) that influence antigenicity of RNA-containing antigens. Since Yaa was known to be associated with a marginal zone defect and generalized B-cell hyper-reactivity [10], Pisitkun and colleagues rea ...
... sera strongly reacted with RNA-containing antigens [9]. Thus, it appears that the Yaa locus bears gene(s) that influence antigenicity of RNA-containing antigens. Since Yaa was known to be associated with a marginal zone defect and generalized B-cell hyper-reactivity [10], Pisitkun and colleagues rea ...
Identificatio of CT521 as a Frequent Target of Th1 Cells in Patients
... analyzed by measuring the IFN-g response to 30 narrow protein fractions with a minimal overlap between neighboring fractions (figu e 1). PBMCs from a total of 16 patients were stimulated with the panel of protein fractions (data not shown). The immune responses showed a marked heterogeneity, and in ...
... analyzed by measuring the IFN-g response to 30 narrow protein fractions with a minimal overlap between neighboring fractions (figu e 1). PBMCs from a total of 16 patients were stimulated with the panel of protein fractions (data not shown). The immune responses showed a marked heterogeneity, and in ...
Folie 1 - German Cancer Research Center
... Expression of MHC alleles is co dominant 4 possible combinations of haplotypes are found in the offspring, there being one chance in four that an individual will share both haplotypes with a sibling. ...
... Expression of MHC alleles is co dominant 4 possible combinations of haplotypes are found in the offspring, there being one chance in four that an individual will share both haplotypes with a sibling. ...
Whey and Glutathione - Part III
... with cancer patients showed a regression in some patient's tumors when fed whey protein concentrate at 30 grams per day. Whey and Glutathione New research using whey protein concentrate led researchers to a discovery regarding the relationship between cancerous cells, glutathione (GSH) and whey prot ...
... with cancer patients showed a regression in some patient's tumors when fed whey protein concentrate at 30 grams per day. Whey and Glutathione New research using whey protein concentrate led researchers to a discovery regarding the relationship between cancerous cells, glutathione (GSH) and whey prot ...
Network-based analysis reveals novel gene signatures in peripheral
... there is a huge unmet clinical need to identify disease biomarkers in peripheral blood. Compared to gene level differential expression approaches to identify gene signatures, network analyses provide a biologically intuitive approach which leverages the co-expression patterns in the transcriptome to ...
... there is a huge unmet clinical need to identify disease biomarkers in peripheral blood. Compared to gene level differential expression approaches to identify gene signatures, network analyses provide a biologically intuitive approach which leverages the co-expression patterns in the transcriptome to ...
on MHC & Leukemia Associations in Humans
... Epigenetics Regulatory region polymorphisms Allelic expression differences Epistatic interactions ...
... Epigenetics Regulatory region polymorphisms Allelic expression differences Epistatic interactions ...
University of Groningen Experimental studies on signal transduction
... activated protein kinase). Furthermore MAPKAPK-2 deficient mice show diminished production of IL-6 and TNF-α 41. These effects are thought to be mediated by a mechanism involving mRNA turnover and protein translation. The p38 MAPK/MAPKAPK-2 pathway is crucial to inflammatory cytokine production. 3.2 ...
... activated protein kinase). Furthermore MAPKAPK-2 deficient mice show diminished production of IL-6 and TNF-α 41. These effects are thought to be mediated by a mechanism involving mRNA turnover and protein translation. The p38 MAPK/MAPKAPK-2 pathway is crucial to inflammatory cytokine production. 3.2 ...
Human immune responses to Plasmodium
... IL-6 family gene (oncostatin M), both of which induce TH17 immune response. This study also observed an elevation in the expression of interferon gamma, NK cell cytotoxicity, antibody-dependent cell cytotoxicity (ADCC), which are triggered by interferon alpha/beta (THαβ), instead of IL-12 (TH1). The ...
... IL-6 family gene (oncostatin M), both of which induce TH17 immune response. This study also observed an elevation in the expression of interferon gamma, NK cell cytotoxicity, antibody-dependent cell cytotoxicity (ADCC), which are triggered by interferon alpha/beta (THαβ), instead of IL-12 (TH1). The ...
Hepatocytes: a key cell type for innate immunity
... patients.23 However, whether CRP inhibits or promotes systemic inflammatory response syndrome in patients with alcoholic hepatitis remains obscure. SAA is a group of four genes with multiple functions from lipid metabolism to immunity in humans. SAA1 and SAA2 are acute response genes that are collec ...
... patients.23 However, whether CRP inhibits or promotes systemic inflammatory response syndrome in patients with alcoholic hepatitis remains obscure. SAA is a group of four genes with multiple functions from lipid metabolism to immunity in humans. SAA1 and SAA2 are acute response genes that are collec ...
Arthritis Res. Ther. 13:R129 - Centre for Microbial Diseases and
... suppressing certain pro-inflammatory pathways, upregulating anti-inflammatory mechanisms (for example, IL10), and intervening in the activation of nuclear factor (NF)-B via multiple mechanisms [3]. A broad spectrum of cationic HDPs are expressed in human synovium tissues with differential expressio ...
... suppressing certain pro-inflammatory pathways, upregulating anti-inflammatory mechanisms (for example, IL10), and intervening in the activation of nuclear factor (NF)-B via multiple mechanisms [3]. A broad spectrum of cationic HDPs are expressed in human synovium tissues with differential expressio ...
How some mycoplasmas evade host immune responses. Microbe 2
... membranes of these wall-less bacteria. These requirements reflect their relative genomic simplicity. The 580-kb genome of Mycoplasma genitalium, for example, is comparable in size to that of a large virus. Nonetheless, this highly successful group containing both commensals and pathogens withstands ...
... membranes of these wall-less bacteria. These requirements reflect their relative genomic simplicity. The 580-kb genome of Mycoplasma genitalium, for example, is comparable in size to that of a large virus. Nonetheless, this highly successful group containing both commensals and pathogens withstands ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008, p-ISSN:2319-7676.
... of 100 uL cell suspension was spread over the glass slide, air-dried and fixed with absolute methanol. The slides were then treated with 3% H2O2 in distilled water for 5 min to block endogenous peroxidase, then washed an additional 3 times with distilled water. Subsequently, 100 µl of the 49,6 kDa s ...
... of 100 uL cell suspension was spread over the glass slide, air-dried and fixed with absolute methanol. The slides were then treated with 3% H2O2 in distilled water for 5 min to block endogenous peroxidase, then washed an additional 3 times with distilled water. Subsequently, 100 µl of the 49,6 kDa s ...
Respiratory and Metabolic Impacts of Crustacean Immunity: Are
... 1983), but hemolymph is distributed rapidly along specific circuits and oxygenation is highly efficient (Pirow et al. 1999, 2004; McMahon 2001). Simple crustaceans often have body segments equipped with unspecialized phyllopod appendages that are used primarily for filter-feeding and/or locomotion b ...
... 1983), but hemolymph is distributed rapidly along specific circuits and oxygenation is highly efficient (Pirow et al. 1999, 2004; McMahon 2001). Simple crustaceans often have body segments equipped with unspecialized phyllopod appendages that are used primarily for filter-feeding and/or locomotion b ...
- Journal of Clinical Investigation
... factors generally bind DNA as homo- or heterodimers. The HLH domain is required for the protein-protein interactions involved in dimerization, while the adjacent basic domain interacts directly with DNA. HLH transcription factors have been shown to be involved in the regulation of transcription of s ...
... factors generally bind DNA as homo- or heterodimers. The HLH domain is required for the protein-protein interactions involved in dimerization, while the adjacent basic domain interacts directly with DNA. HLH transcription factors have been shown to be involved in the regulation of transcription of s ...
A Cyclin A-Protein Kinase Complex Possesses
... There are some indications that cyclin A, originally identified as a mitotic cyclin, may function in S phase. In mammalian cells, the cyclin A protein and its associated kinase activity appear during S phase, do not show as dramatic a peak in mitosis as cyclin B, and disappear prior to the disappear ...
... There are some indications that cyclin A, originally identified as a mitotic cyclin, may function in S phase. In mammalian cells, the cyclin A protein and its associated kinase activity appear during S phase, do not show as dramatic a peak in mitosis as cyclin B, and disappear prior to the disappear ...
Challenges and strategies: The immune responses in gene therapy
... genome is always comprised of gene promoters and (or) exons. CpGs within CpG islands are normally unmethylated whereas most CpGs outside CpG islands are methylated. The genomic DNAs of bacteria and vertebrates differ from the frequency and methylation of CpGs, which are relatively common in bacteria ...
... genome is always comprised of gene promoters and (or) exons. CpGs within CpG islands are normally unmethylated whereas most CpGs outside CpG islands are methylated. The genomic DNAs of bacteria and vertebrates differ from the frequency and methylation of CpGs, which are relatively common in bacteria ...
A profile of fertilization in mammals
... EBPs? It is now generally held that, for a given species, more than one kind of EBP may be involved in binding of sperm to eggs. In addition, it is often proposed that sperm from different mammalian species use different EBPs during fertilization. This situation has been described recently29 as refl ...
... EBPs? It is now generally held that, for a given species, more than one kind of EBP may be involved in binding of sperm to eggs. In addition, it is often proposed that sperm from different mammalian species use different EBPs during fertilization. This situation has been described recently29 as refl ...
Vibrio Amalia A. Almada, Ann M. Tarrant#
... and the dramatic impacts of these associations on the proliferation, virulence and physiology of vibrios (Kirn, Jude and Taylor 2005; Colwell 2009). However, whether copepods are in turn impacted by or further regulate colonizing vibrios is unknown. In light of copepods’ abundance across aquatic hab ...
... and the dramatic impacts of these associations on the proliferation, virulence and physiology of vibrios (Kirn, Jude and Taylor 2005; Colwell 2009). However, whether copepods are in turn impacted by or further regulate colonizing vibrios is unknown. In light of copepods’ abundance across aquatic hab ...
Epigenetic Regulation of Innate Immunity by microRNAs
... cells. Upon transfection of these cells with Dicer-specific siRNA, increased accumulation of let-7 precursor products were observed [6]. The RNA binding protein Lin28, which was identified as an inhibitor of pri-miRNA processing, binds to different members of let-7 precursor miRNAs and prevents proc ...
... cells. Upon transfection of these cells with Dicer-specific siRNA, increased accumulation of let-7 precursor products were observed [6]. The RNA binding protein Lin28, which was identified as an inhibitor of pri-miRNA processing, binds to different members of let-7 precursor miRNAs and prevents proc ...
honours thesis - University of Canberra
... functional groups, such as phosphor groups, to peptides residues of the histone tail can aid/obstruct in the recruitment of enhancers, repressors and chromatin remodelers. Histone phosphorylation has been implicated in the regulation of gene transcription in eukaryotic cells. Protein Kinas C β (PKCβ ...
... functional groups, such as phosphor groups, to peptides residues of the histone tail can aid/obstruct in the recruitment of enhancers, repressors and chromatin remodelers. Histone phosphorylation has been implicated in the regulation of gene transcription in eukaryotic cells. Protein Kinas C β (PKCβ ...
Reproduction - Male
... Prevents large molecules such as antibodies and large amounts of fat soluble vitamins from passing in large amounts from mother to young. Viruses that are small enough to penetrate the Placental Barrier can cause defects in young. ...
... Prevents large molecules such as antibodies and large amounts of fat soluble vitamins from passing in large amounts from mother to young. Viruses that are small enough to penetrate the Placental Barrier can cause defects in young. ...
Transplant Outcome in Mice Effects of T Cell Frequency and Graft
... The features that determine whether graft-reactive T lymphocytes develop into effector cells capable of mediating organ destruction are not well understood. To investigate potential factors involved in this process, we first confirmed that female recipient mice acutely rejected minor Ag-disparate ma ...
... The features that determine whether graft-reactive T lymphocytes develop into effector cells capable of mediating organ destruction are not well understood. To investigate potential factors involved in this process, we first confirmed that female recipient mice acutely rejected minor Ag-disparate ma ...
Drosophila melanogaster
Drosophila melanogaster is a species of fly (the taxonomic order Diptera) in the family Drosophilidae. The species is known generally as the common fruit fly or vinegar fly. Starting with Charles W. Woodworth's proposal of the use of this species as a model organism, D. melanogaster continues to be widely used for biological research in studies of genetics, physiology, microbial pathogenesis, and life history evolution. It is typically used because it is an animal species that is easy to care for, has four pairs of chromosomes, breeds quickly, and lays many eggs. D. melanogaster is a common pest in homes, restaurants, and other occupied places where food is served.Flies belonging to the family Tephritidae are also called ""fruit flies"". This can cause confusion, especially in Australia and South Africa, where the Mediterranean fruit fly Ceratitis capitata is an economic pest.