Kidney Function Testing
... Increased excretion of low molecular weight proteins can occur with marked overproduction of a particular protein, leading to increased glomerular filtration and excretion of this protein This is due to (almost all causes): 1- Immunoglobulin light chains in multiple myeloma 2- lysozymes in acute ...
... Increased excretion of low molecular weight proteins can occur with marked overproduction of a particular protein, leading to increased glomerular filtration and excretion of this protein This is due to (almost all causes): 1- Immunoglobulin light chains in multiple myeloma 2- lysozymes in acute ...
The Metamorphosis of the Aleurone Protein Storage Vacuole
... (GA) and abscisic acid (ABA), but more recent investigations have focused on how these hormones regulate gene expression (Fincher, 1989 ; Jacobsen, Gubler and Chandler, 1995 ; Bethke, Schuurink and Jones, 1997). This approach has been eminently successful, and a great deal is known about how GA and ...
... (GA) and abscisic acid (ABA), but more recent investigations have focused on how these hormones regulate gene expression (Fincher, 1989 ; Jacobsen, Gubler and Chandler, 1995 ; Bethke, Schuurink and Jones, 1997). This approach has been eminently successful, and a great deal is known about how GA and ...
Plasma membrane - HCC Learning Web
... • 3-1 List the functions of the plasma membrane and the structural features that enable it to perform those functions. • 3-2 Describe the organelles of a typical cell, and indicate the specific functions of each. • 3-3 Explain the functions of the cell nucleus and discuss the nature and importance o ...
... • 3-1 List the functions of the plasma membrane and the structural features that enable it to perform those functions. • 3-2 Describe the organelles of a typical cell, and indicate the specific functions of each. • 3-3 Explain the functions of the cell nucleus and discuss the nature and importance o ...
Discovering the Interaction Propensities of Amino Acids and
... considered for forming 4 hydrogen bonds in this study were: contacts with a maximum D-A distance of 3.9 Å, maximum H-A distance of 2.5 Å, and minimum D-H-A and H-A-AA angels set to 90, where AA is an acceptor antecedent. Water-mediated ligand interactions are essential in biological processes. The p ...
... considered for forming 4 hydrogen bonds in this study were: contacts with a maximum D-A distance of 3.9 Å, maximum H-A distance of 2.5 Å, and minimum D-H-A and H-A-AA angels set to 90, where AA is an acceptor antecedent. Water-mediated ligand interactions are essential in biological processes. The p ...
Mechanisms and applications of disulfide bond formation
... these proteins contain disulfide bonds in their native state, covalent links formed between the thiol groups of cysteine residues. In many proteins, disulfide bonds play an essential role in folding, stabilizing structure and the function of the protein. Therefore, understanding the pathways of disu ...
... these proteins contain disulfide bonds in their native state, covalent links formed between the thiol groups of cysteine residues. In many proteins, disulfide bonds play an essential role in folding, stabilizing structure and the function of the protein. Therefore, understanding the pathways of disu ...
Protein Creation Pathway
... The Nucleolus The nucleolus is a dense region within the nucleus. This organelle (tiny cell part) is responsible for creating thousands of ribosomes. Ribosomes play an important role in the creation of proteins as we will see later. Click the Nucleolus to see an artists drawing. ...
... The Nucleolus The nucleolus is a dense region within the nucleus. This organelle (tiny cell part) is responsible for creating thousands of ribosomes. Ribosomes play an important role in the creation of proteins as we will see later. Click the Nucleolus to see an artists drawing. ...
INTEINS: Structure, Function, and Evolution
... Among other data it lists the inteins’ sequences, conserved motifs, host organisms, and host proteins. More than 130 inteins are known in 34 different types of proteins (68, 76, 77). The inteins are between 134 and 608 amino acids long, and they are found in members of all three domains of life: Euk ...
... Among other data it lists the inteins’ sequences, conserved motifs, host organisms, and host proteins. More than 130 inteins are known in 34 different types of proteins (68, 76, 77). The inteins are between 134 and 608 amino acids long, and they are found in members of all three domains of life: Euk ...
The DNA Binding Properties of Saccharomyces cerevisiae Rad51
... secondary structures in ssDNA thereby inhibiting formation of a contiguous nucleoprotein filament; during synapsis, Rad51 protein inactivates the homologous dsDNA partner by directly binding to it. ...
... secondary structures in ssDNA thereby inhibiting formation of a contiguous nucleoprotein filament; during synapsis, Rad51 protein inactivates the homologous dsDNA partner by directly binding to it. ...
Chapter 14 Preparing Semisynthetic and Fully Synthetic Histones
... Chemical ligation is an excellent way to prepare the homogenous samples of precisely modified histone proteins that are necessary to characterize the molecular functions of these modifications within the structured nucleosome core (5). Native chemical ligation (NCL) is the chemoselective condensatio ...
... Chemical ligation is an excellent way to prepare the homogenous samples of precisely modified histone proteins that are necessary to characterize the molecular functions of these modifications within the structured nucleosome core (5). Native chemical ligation (NCL) is the chemoselective condensatio ...
Sequence and Tissue Distribution of a Second Protein of Hepatic
... Quantitation of message levels for Cx32 and Cx26 in mouse and rat liver used similar autoradiogmms to those in Fig. 7 (see Nicholson and Zhang, 1988). Equivalent levels of total RNA, as determined by A260were loaded on the original glyoxal gel, and ethidium bromide staining was used after blotting t ...
... Quantitation of message levels for Cx32 and Cx26 in mouse and rat liver used similar autoradiogmms to those in Fig. 7 (see Nicholson and Zhang, 1988). Equivalent levels of total RNA, as determined by A260were loaded on the original glyoxal gel, and ethidium bromide staining was used after blotting t ...
Infant Formula 2015
... Factors controlling drug transfer into breast milk 1. Buffer systems: • Milk is buffered by citrate and phosphate, with a limited buffer capacity • Milk pH tends to be lower than that in plasma: 7.0-7.25 • the pH difference between milk and plasma has a substantial effect on the M/P ratio. ...
... Factors controlling drug transfer into breast milk 1. Buffer systems: • Milk is buffered by citrate and phosphate, with a limited buffer capacity • Milk pH tends to be lower than that in plasma: 7.0-7.25 • the pH difference between milk and plasma has a substantial effect on the M/P ratio. ...
The ins and outs of sphingolipid synthesis
... belongs to a family of pyridoxal 5 0 -phosphate-dependent a-oxoamine synthases, and is composed of a heterodimer of two similar, but non-identical, subunits, named Lcb1p (long chain base 1) and Lcb2p, which were first identified in yeast by genetic methods [7,8]. Eukaryotic SPTs are membrane bound, ...
... belongs to a family of pyridoxal 5 0 -phosphate-dependent a-oxoamine synthases, and is composed of a heterodimer of two similar, but non-identical, subunits, named Lcb1p (long chain base 1) and Lcb2p, which were first identified in yeast by genetic methods [7,8]. Eukaryotic SPTs are membrane bound, ...
αII-Spectrin interacts with Tes and EVL, two actin
... attached to diverse cellular membranes, is presumed to be involved in the stabilization of membranes, the establishment of membrane domains as well as in vesicle trafficking and nuclear functions. Spectrin tetramers made of α- and β-subunits are linked to actin microfilaments, forming a network that ...
... attached to diverse cellular membranes, is presumed to be involved in the stabilization of membranes, the establishment of membrane domains as well as in vesicle trafficking and nuclear functions. Spectrin tetramers made of α- and β-subunits are linked to actin microfilaments, forming a network that ...
... The interaction that is being disrupted by the salt is electrostatic, positively charged Lys and/or Arg residues on the protein are interacting with negatively charged phosphate groups on the DNA ( 3 1/2 pts). The protein still binds with high salt, so there must be additional interactions, i.e. hyd ...
Gonococcal outer-membrane protein PIB
... those from other serovars reveals that sequence variations occur in two discrete regions of the molecule centred on residues 1% (Varl) and 237 (Var2). A series of peptides spanning the amino acid sequence of the protein were synthesized on solid-phase supports and reacted with a panel of monoclonal ...
... those from other serovars reveals that sequence variations occur in two discrete regions of the molecule centred on residues 1% (Varl) and 237 (Var2). A series of peptides spanning the amino acid sequence of the protein were synthesized on solid-phase supports and reacted with a panel of monoclonal ...
Gloria Jih Biophysics 205 Semester Proposal Title: Global analysis
... In yeast, myosin moving along actin filaments seems to be the only mechanism for mRNA transport. In higher eukaryotes, kinesin and dynein motors moving along microtubules plays the major role. For example, kinesin-1 is responsible for the transport of myelin basic protein (MBP) mRNA in oligodendrocy ...
... In yeast, myosin moving along actin filaments seems to be the only mechanism for mRNA transport. In higher eukaryotes, kinesin and dynein motors moving along microtubules plays the major role. For example, kinesin-1 is responsible for the transport of myelin basic protein (MBP) mRNA in oligodendrocy ...
Structural characterization of L
... have been identified from various microorganisms: l-lysine-a-oxidase [14], l-phenylalanine oxidase [15,16], and l-aspartate oxidase [17]. These enzymes are considered to have an identical catalytic mechanism but different affinities for amino acid substrates. In this class of enzymes, the crystal stru ...
... have been identified from various microorganisms: l-lysine-a-oxidase [14], l-phenylalanine oxidase [15,16], and l-aspartate oxidase [17]. These enzymes are considered to have an identical catalytic mechanism but different affinities for amino acid substrates. In this class of enzymes, the crystal stru ...
Yeast
... electrophoresis with two pI ranges: 5·14–4·0 and 1·6–1·8. The enzyme preparation had a remarkably strong stability at pH 6·0–7·0, surviving boiling for 10 min without losing more than 60% of activity. On Western blots, this enzyme was recognized by antibodies raised in rabbits against D. hansenii ex ...
... electrophoresis with two pI ranges: 5·14–4·0 and 1·6–1·8. The enzyme preparation had a remarkably strong stability at pH 6·0–7·0, surviving boiling for 10 min without losing more than 60% of activity. On Western blots, this enzyme was recognized by antibodies raised in rabbits against D. hansenii ex ...
structure and function of bio- molecules - Aditya K Panda, PhD
... proteins. They are linked together by a peptide bond (see later). ...
... proteins. They are linked together by a peptide bond (see later). ...
Characterization of the Cytosolic Proteins Involved in the Amoeboid
... Two of the proteins had reciprocal effects on fiber growth: p38 increased fiber growth rate and p16 decreased fiber growth rate. The effects of both p38 and p16 were concentration-dependent and antagonistic. Since the rate-enhancement by p38 was not potentiated by MSP, another cytosolic protein is i ...
... Two of the proteins had reciprocal effects on fiber growth: p38 increased fiber growth rate and p16 decreased fiber growth rate. The effects of both p38 and p16 were concentration-dependent and antagonistic. Since the rate-enhancement by p38 was not potentiated by MSP, another cytosolic protein is i ...
DNA Specificity of the Bicoid Activator Protein Is Determined by
... Here we show that DNA-bound LexA-Bicoid and GAL4Bicoid fusion proteins stimulate gene expression in yeast. Bicoid fusion proteins activated target genes that carried upstream LexA operators, GAL4 sites, or Bicoid binding sites. We used the gene activation phenotype to establish which residues in the ...
... Here we show that DNA-bound LexA-Bicoid and GAL4Bicoid fusion proteins stimulate gene expression in yeast. Bicoid fusion proteins activated target genes that carried upstream LexA operators, GAL4 sites, or Bicoid binding sites. We used the gene activation phenotype to establish which residues in the ...
Henikoff, S. and Henikoff, Jorja G. Amino Acid Substitution Matrices from Protein Blocks. Proc. Natl. Acad. Sci. USA , 89, pp. 10915-10919, 1992.
... Amongthemostusefulcomputer-basedtools in modern residue, there are 8 + 7 + . . . 1 = 36 possible AA pairs, 9 biology are those that involve sequence alignments of proAS or SA pairs, and no SS pairs. Counts of all possible pairs teins, since these alignments often provide important insights in each c ...
... Amongthemostusefulcomputer-basedtools in modern residue, there are 8 + 7 + . . . 1 = 36 possible AA pairs, 9 biology are those that involve sequence alignments of proAS or SA pairs, and no SS pairs. Counts of all possible pairs teins, since these alignments often provide important insights in each c ...
Phytochemistry
... Key Word Inde~Amaranthus leucocarpus; Amaranthaceae; lectin; agglutinin; stroma columns; erythrocytes. Abstract--A lectin of M, ca 45,000 per subunit from Amaranthus leucocarpus seeds, has been isolated and purified by affinity chromatography using a stroma column. It is a glycoprotein (10% w/w carb ...
... Key Word Inde~Amaranthus leucocarpus; Amaranthaceae; lectin; agglutinin; stroma columns; erythrocytes. Abstract--A lectin of M, ca 45,000 per subunit from Amaranthus leucocarpus seeds, has been isolated and purified by affinity chromatography using a stroma column. It is a glycoprotein (10% w/w carb ...
The molecular machinery of Keilin`s respiratory chain
... binding. This has been aided recently with the recognition that some parts of the structure are likely to have evolved from pre-existing modules that were incorporated into the complex [25,30]. It is clear that the 51 kDa subunit binds FMN and has the NADH-binding site and also houses one of the Fe4 ...
... binding. This has been aided recently with the recognition that some parts of the structure are likely to have evolved from pre-existing modules that were incorporated into the complex [25,30]. It is clear that the 51 kDa subunit binds FMN and has the NADH-binding site and also houses one of the Fe4 ...
Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L
... constructed at the initial stage and fast oil accumulation stage of seed development. These reads were assembled into 70,392 unigenes; 22,179 unigenes showed a 2-fold or greater expression difference between the two libraries. Using this data we identified unigenes that may be involved in de novo FA ...
... constructed at the initial stage and fast oil accumulation stage of seed development. These reads were assembled into 70,392 unigenes; 22,179 unigenes showed a 2-fold or greater expression difference between the two libraries. Using this data we identified unigenes that may be involved in de novo FA ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.