Formate Dehydrogenase, Molecular Modeling and Docking with
... Wu et al., 2013). It also has been used in production of other chemicals such as L-lactic acid and aromatic alpha-keto esters (Jayabalan et al., 2012; Kratzer et al., 2008). FDH is widely presented in methanol-assimilating bacteria and yeasts. It was produced from Pseudomonas sp. 101 (PseFDH), which ...
... Wu et al., 2013). It also has been used in production of other chemicals such as L-lactic acid and aromatic alpha-keto esters (Jayabalan et al., 2012; Kratzer et al., 2008). FDH is widely presented in methanol-assimilating bacteria and yeasts. It was produced from Pseudomonas sp. 101 (PseFDH), which ...
Canola Meal for Pig Feeding
... project work undertaken jointly by the Pork CRC and Australian Oilseeds Federation. This work was able to demonstrate that under typical processing conditions as used by GrainCorp Oilseeds, the effects of processing have a minimal impact upon meal quality. It is during more extreme or less controlle ...
... project work undertaken jointly by the Pork CRC and Australian Oilseeds Federation. This work was able to demonstrate that under typical processing conditions as used by GrainCorp Oilseeds, the effects of processing have a minimal impact upon meal quality. It is during more extreme or less controlle ...
EVOLUTIONARILY RELATED INSERTION PATHWAYS OF
... membranes appears to be different in that it involves no SecYE-like components. A conservative pathway, recently identified in mitochondria, involves the Oxa1 protein for the insertion of proteins from the matrix. The presence of Oxa1 homologues in eubacteria and chloroplasts suggests that this path ...
... membranes appears to be different in that it involves no SecYE-like components. A conservative pathway, recently identified in mitochondria, involves the Oxa1 protein for the insertion of proteins from the matrix. The presence of Oxa1 homologues in eubacteria and chloroplasts suggests that this path ...
Biochemical studies on a versatile esterase that is most catalytically
... Because the active clone with CN1E1 actively hydrolysed naphthalene carboxylic acid methyl ester on an agar plate (Fig. 1), we further tested and quantified hydrolysis for the pure enzyme using this and other model PAH esters; the pH-indicator protocol (at 40°C and pH 8.0) used to examine hydrolysis ...
... Because the active clone with CN1E1 actively hydrolysed naphthalene carboxylic acid methyl ester on an agar plate (Fig. 1), we further tested and quantified hydrolysis for the pure enzyme using this and other model PAH esters; the pH-indicator protocol (at 40°C and pH 8.0) used to examine hydrolysis ...
Sequence - BIOTEC - Biotechnology Center TU Dresden
... clues about their functions Screen for similar proteins sequences with known structure [15] If any are found Then interpret difference (homology modelling) [25] Else predict structure from sequence [55] ...
... clues about their functions Screen for similar proteins sequences with known structure [15] If any are found Then interpret difference (homology modelling) [25] Else predict structure from sequence [55] ...
List of abbreviations
... Patients submitted to invasive procedures or admitted into intensive care units (ICU) become more susceptible to acquire nosocomial infections in comparison to other hospitalized patients. Implantable medical apparatus, such as urinary catheters, central venous and arterial catheters and endotrachea ...
... Patients submitted to invasive procedures or admitted into intensive care units (ICU) become more susceptible to acquire nosocomial infections in comparison to other hospitalized patients. Implantable medical apparatus, such as urinary catheters, central venous and arterial catheters and endotrachea ...
Effects of mutation on key amino acid residues in
... stabilizes p53 from degradation by MDM2 mediated pathway [3]. The central DNA binding core domain (amino acid residues 102-292), most conserved among all the domains is responsible for sequence specific binding to DNA and it is characterized by two copies of 10 bp consensus sequence 5’-PuPuPuC(A/T)- ...
... stabilizes p53 from degradation by MDM2 mediated pathway [3]. The central DNA binding core domain (amino acid residues 102-292), most conserved among all the domains is responsible for sequence specific binding to DNA and it is characterized by two copies of 10 bp consensus sequence 5’-PuPuPuC(A/T)- ...
The Structure of the PanD/PanZ Protein Complex
... cleavage of the DNA-binding protein umuD (McDonald et al., 1998), and autoproteolysis of SEA domains (Johannson et al., 2009; Levitin et al., 2005). In most cases these post-translational modifications are autocatalytic, but the reaction rates of a subset of enzymes have been shown to be enhanced by ...
... cleavage of the DNA-binding protein umuD (McDonald et al., 1998), and autoproteolysis of SEA domains (Johannson et al., 2009; Levitin et al., 2005). In most cases these post-translational modifications are autocatalytic, but the reaction rates of a subset of enzymes have been shown to be enhanced by ...
FtsZ - Cytoskeleton, Inc.
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
Elucidating Substrate and Inhibitor Binding Sites on the Surface of
... Fig. 1. The 89–95 loop defines a substrate binding subsite for GSK-3β. (a) Features of the substrate binding site of GSK-3β. The structure of GSK-3β is based on the available crystal structures,30,40 as described in Ref. 39. The surface of GSK-3β is shown in gray, with the loop 89–95 shown in yellow ...
... Fig. 1. The 89–95 loop defines a substrate binding subsite for GSK-3β. (a) Features of the substrate binding site of GSK-3β. The structure of GSK-3β is based on the available crystal structures,30,40 as described in Ref. 39. The surface of GSK-3β is shown in gray, with the loop 89–95 shown in yellow ...
Ultrasonic Absorption Mechanisms in Aqueous Solutions of Bovine
... the former, and not present in the latter, which may also possess relaxational behavior. A similar situation has been reported for bovine serum albumin.22 The composite ultrasonic absorption titration curve is shown in Figure 2 for the frequency range 8.9-50.5 MHz and over the pH range 1.5-12.3. Sim ...
... the former, and not present in the latter, which may also possess relaxational behavior. A similar situation has been reported for bovine serum albumin.22 The composite ultrasonic absorption titration curve is shown in Figure 2 for the frequency range 8.9-50.5 MHz and over the pH range 1.5-12.3. Sim ...
Word
... Pyridoxal 5'-phosphate (PLP) is an essential cofactor for nearly 60 Escherichia coli enzymes but ...
... Pyridoxal 5'-phosphate (PLP) is an essential cofactor for nearly 60 Escherichia coli enzymes but ...
Deciphering the molecular basis of the specificity of protein
... determine their amino acid preferences. Two datasets have been examined. Firstly, one composed of non-covalently bound carbohydrates ligands. The results of this analysis is compared to the second dataset, obtained from the study of the spatial vicinity of the monosaccharides that form the common st ...
... determine their amino acid preferences. Two datasets have been examined. Firstly, one composed of non-covalently bound carbohydrates ligands. The results of this analysis is compared to the second dataset, obtained from the study of the spatial vicinity of the monosaccharides that form the common st ...
Understanding the acetylome: translating targeted
... activity of numerous metabolic proteins, in addition to substrate transporters, receptors, and scaffolding proteins. Furthermore, the functional specificity of acetylation was highlighted, as identified acetylation sites were frequently located in regions with ordered secondary structure. When acety ...
... activity of numerous metabolic proteins, in addition to substrate transporters, receptors, and scaffolding proteins. Furthermore, the functional specificity of acetylation was highlighted, as identified acetylation sites were frequently located in regions with ordered secondary structure. When acety ...
Capacitation-associated Changes in Protein
... mammalian sperm can be capacitated in vitro by using a defined medium, the in vitro capacitation system has been used to understand the molecular basis of capacitation. It has been shown that certain components in the medium promote capacitation, such as Ca2+, HCO3- and serum albumin (as the primary ...
... mammalian sperm can be capacitated in vitro by using a defined medium, the in vitro capacitation system has been used to understand the molecular basis of capacitation. It has been shown that certain components in the medium promote capacitation, such as Ca2+, HCO3- and serum albumin (as the primary ...
Translation
... • During and after synthesis, a polypeptide chain spontaneously coils and folds into its threedimensional shape • Proteins may also require post-translational modifications before doing their job • Some polypeptides are activated by enzymes that cleave them • Other polypeptides come together to form ...
... • During and after synthesis, a polypeptide chain spontaneously coils and folds into its threedimensional shape • Proteins may also require post-translational modifications before doing their job • Some polypeptides are activated by enzymes that cleave them • Other polypeptides come together to form ...
the PDF - JILA - University of Colorado Boulder
... dressed states jm0 i and jm1 i out of bare rotor states using microwave fields. This, in turn, allows for the full controllability of Jz , J? , V, and W, which is one of the main results of the present Letter. Lattice Hamiltonians based on more than one molecular rotational state have been consider ...
... dressed states jm0 i and jm1 i out of bare rotor states using microwave fields. This, in turn, allows for the full controllability of Jz , J? , V, and W, which is one of the main results of the present Letter. Lattice Hamiltonians based on more than one molecular rotational state have been consider ...
Identification of a novel N-terminal hydrophobic sequence that
... is the proline knot, a ~72 amino acid hydrophobic sequence found in the middle of plant oleosins. The proline knot is thought to direct the protein to the droplet from its site of insertion in the ER, leaving flanking hydrophilic residues in the cytoplasm. The flanking sequences may also participate ...
... is the proline knot, a ~72 amino acid hydrophobic sequence found in the middle of plant oleosins. The proline knot is thought to direct the protein to the droplet from its site of insertion in the ER, leaving flanking hydrophilic residues in the cytoplasm. The flanking sequences may also participate ...
Structure and function of the eukaryotic ADP
... extensively tested by 31P-NMR, where the enzyme proved to be highly specific for Dglucose. Residues important for catalysis have been modified by site-directed mutagenesis and the variants of H. sapiens ADPGK were purified and kinetic parameters determined. A single crystal was obtained from a trunc ...
... extensively tested by 31P-NMR, where the enzyme proved to be highly specific for Dglucose. Residues important for catalysis have been modified by site-directed mutagenesis and the variants of H. sapiens ADPGK were purified and kinetic parameters determined. A single crystal was obtained from a trunc ...
Translation - Advanced
... into secondary and then tertiary structures. These levels of organization were discussed in the Organic Compounds: Proteins (Advanced) concept. Briefly, the primary structure of the protein is the sequence of amino acids determined by the gene and mRNA. The secondary and tertiary structures are dete ...
... into secondary and then tertiary structures. These levels of organization were discussed in the Organic Compounds: Proteins (Advanced) concept. Briefly, the primary structure of the protein is the sequence of amino acids determined by the gene and mRNA. The secondary and tertiary structures are dete ...
Utilisation of Whey
... IgA, IgG, IgE and IgM, with molecular weights of 150,000 to 450,000 or greater. Some of them represent the normal antibody components of the cow and are the same as those found in the bloodstream; however, there is a secretory form of IgA which is produced in the mammary gland in response to infecti ...
... IgA, IgG, IgE and IgM, with molecular weights of 150,000 to 450,000 or greater. Some of them represent the normal antibody components of the cow and are the same as those found in the bloodstream; however, there is a secretory form of IgA which is produced in the mammary gland in response to infecti ...
NHS ester - BroadPharm
... suitable for modification of amino groups which exist in proteins, peptides, amino-biomolecules, and DNA. The reaction of NHS esters with amines is pH-dependent. At low pH, the amino group is protonated, and no modification takes place. At pH that is higher than optimal, the quick hydrolysis of NHS ...
... suitable for modification of amino groups which exist in proteins, peptides, amino-biomolecules, and DNA. The reaction of NHS esters with amines is pH-dependent. At low pH, the amino group is protonated, and no modification takes place. At pH that is higher than optimal, the quick hydrolysis of NHS ...
A Mutation in the Anticodon of a Single tRNA Is
... were more rounded with shortened petioles, but it is distinct in that it appears to retain sensitivity to ethylene and the mutation is homozygous lethal. Surprisingly, positional cloning on the basis of 2,4-D resistance revealed that the mutation linked to the observed phenotypes was not found in an ...
... were more rounded with shortened petioles, but it is distinct in that it appears to retain sensitivity to ethylene and the mutation is homozygous lethal. Surprisingly, positional cloning on the basis of 2,4-D resistance revealed that the mutation linked to the observed phenotypes was not found in an ...
subset seed extension to protein blast
... written in C++, relies on the template mechanism, and is prone to compiler optimizations (most functions can be inlined). An important advantage of our implementation is that being developed within the NCBI-BLAST framework, it inherits all stable and tested features of this implementation. The first ...
... written in C++, relies on the template mechanism, and is prone to compiler optimizations (most functions can be inlined). An important advantage of our implementation is that being developed within the NCBI-BLAST framework, it inherits all stable and tested features of this implementation. The first ...
Automated extraction of mutation data from the literature: application
... terms with their true contextual meanings. For example, the term ‘T47D’ will be ignored if it is found close to the terms ‘cell line’, ‘tumour’, ‘tumor’ or ‘cancer’. This list will have to be updated manually as other point mutation-like terms are detected in the documents subsequently processed. Ho ...
... terms with their true contextual meanings. For example, the term ‘T47D’ will be ignored if it is found close to the terms ‘cell line’, ‘tumour’, ‘tumor’ or ‘cancer’. This list will have to be updated manually as other point mutation-like terms are detected in the documents subsequently processed. Ho ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.