Ion exchange chromatography File
... Resins with low degree of cross-linking are more permeable to high molecular weight compounds, but they are less rigid and swell more when placed in buffer Sulphonation of cross-linked polystyrene results in sulphonated polystyrene resin such as Dowex 50- strong acidic exchanger Basic exchangers are ...
... Resins with low degree of cross-linking are more permeable to high molecular weight compounds, but they are less rigid and swell more when placed in buffer Sulphonation of cross-linked polystyrene results in sulphonated polystyrene resin such as Dowex 50- strong acidic exchanger Basic exchangers are ...
The QIAexpressionist™
... the tag is small, uncharged, and therefore does not generally affect secretion, compartmentalization, or folding of the fusion protein within the cell. In most cases, the 6xHis tag does not interfere with the structure or function of the purified protein as demonstrated for a wide variety of protein ...
... the tag is small, uncharged, and therefore does not generally affect secretion, compartmentalization, or folding of the fusion protein within the cell. In most cases, the 6xHis tag does not interfere with the structure or function of the purified protein as demonstrated for a wide variety of protein ...
Document
... Monomer: nucleotide (made of 3 parts) a. 5 carbon sugar b. Phosphate group c. Nitrogen base ...
... Monomer: nucleotide (made of 3 parts) a. 5 carbon sugar b. Phosphate group c. Nitrogen base ...
The OSBP-related proteins (ORPs) - Biochemical Society Transactions
... been implicated in the function of ERJs (endoplasmic reticulum junctions), it is evident that any single model for their mechanism of action is insufficient. The existing evidence points in many different directions, such as integration of sterol and sphingomyelin metabolism, regulation of neutral li ...
... been implicated in the function of ERJs (endoplasmic reticulum junctions), it is evident that any single model for their mechanism of action is insufficient. The existing evidence points in many different directions, such as integration of sterol and sphingomyelin metabolism, regulation of neutral li ...
Crenarchaeal CdvA Forms Double-Helical Filaments Containing
... microscopy, we evidenced for the first time that CdvA forms polymers in association with DNA, similar to known bacterial DNA partitioning proteins. We also observed that, in contrast to full-lengh CdvB that was purified as a monodisperse protein, the C-terminally deleted CdvB construct forms filamen ...
... microscopy, we evidenced for the first time that CdvA forms polymers in association with DNA, similar to known bacterial DNA partitioning proteins. We also observed that, in contrast to full-lengh CdvB that was purified as a monodisperse protein, the C-terminally deleted CdvB construct forms filamen ...
BIOLOGICAL MACROMOLECULES
... The advanced course on Biological Macromolecules is an introduction to various aspects of structure and function of proteins, nucleic acids, and their complexes. The topics addressed are a selection of modern biophysical methods applied to current questions in macromolecular biochemistry. In particu ...
... The advanced course on Biological Macromolecules is an introduction to various aspects of structure and function of proteins, nucleic acids, and their complexes. The topics addressed are a selection of modern biophysical methods applied to current questions in macromolecular biochemistry. In particu ...
lecture09_09
... • A combination of secondary structural units – Forms basic level of classification ...
... • A combination of secondary structural units – Forms basic level of classification ...
Investigating the effects of different types of mutations
... Message 4 ACCGTAATATACCTC? TGGCATTATATGGAG ...
... Message 4 ACCGTAATATACCTC? TGGCATTATATGGAG ...
Toxic Effects of Nitric Oxide
... Pryor, W., Jin, X., and Squadrito, G. 1994. One and Two Electron Oxidations of Methionine by Peroxynitrite. PNAS 91: 11173-11177. Rogstam, A., Larsson, J. T., Kjelgaard, P., and von Washenfeldt, C. 2007. Mechanism of Adaptation to Nitrosative Stress in Bacillus subtilis. Journal of Bacteriology 189: ...
... Pryor, W., Jin, X., and Squadrito, G. 1994. One and Two Electron Oxidations of Methionine by Peroxynitrite. PNAS 91: 11173-11177. Rogstam, A., Larsson, J. T., Kjelgaard, P., and von Washenfeldt, C. 2007. Mechanism of Adaptation to Nitrosative Stress in Bacillus subtilis. Journal of Bacteriology 189: ...
6b. Thermodynamics
... • Keep in mind that energy released in an exergonic reaction is not necessarily released as heat. an exergonic (energy releasing) reaction is not necessarily exothermic (heat releasing). • a reaction with a negative Δ G (free energy) value may have either a positive or negative ΔH (enthalpy) valu ...
... • Keep in mind that energy released in an exergonic reaction is not necessarily released as heat. an exergonic (energy releasing) reaction is not necessarily exothermic (heat releasing). • a reaction with a negative Δ G (free energy) value may have either a positive or negative ΔH (enthalpy) valu ...
Amino Acid Alphabet
... Designing/engineering an enzyme is difficult because of the precise positioning of residues required for catalysis Chorismate mutase (CM) catalyzes the rearrangement of chorismate to prephenate and is essential for the biosynthesis of aromatic residues The enzyme is a domain-swapped homodimer of thr ...
... Designing/engineering an enzyme is difficult because of the precise positioning of residues required for catalysis Chorismate mutase (CM) catalyzes the rearrangement of chorismate to prephenate and is essential for the biosynthesis of aromatic residues The enzyme is a domain-swapped homodimer of thr ...
Bma: Visual Tool for Modeling and Analyzing Biological Networks
... Thus, a QN defines a transition system over Σ. All variables change their value synchronously by following their target functions. We abuse notation and write also T ∶ Σ → Σ as the function that associates with a state s its successor s′ . Each protein in the design corresponds to a variable in the ...
... Thus, a QN defines a transition system over Σ. All variables change their value synchronously by following their target functions. We abuse notation and write also T ∶ Σ → Σ as the function that associates with a state s its successor s′ . Each protein in the design corresponds to a variable in the ...
In Vitro Protein Synthesis of Perdeuterated Proteins for NMR Studies
... selectively labeled amino acids into polypeptide chains in situations where in vivo protein expression typically results in isotope scrambling or isotope dilution (Kigawa et al. 1995; Ozawa et al. 2004; Waugh 1996), and its use can extend to cytotoxic proteins, such as proteases or apoptosis-related ...
... selectively labeled amino acids into polypeptide chains in situations where in vivo protein expression typically results in isotope scrambling or isotope dilution (Kigawa et al. 1995; Ozawa et al. 2004; Waugh 1996), and its use can extend to cytotoxic proteins, such as proteases or apoptosis-related ...
protein metabolism - cmb
... individual molecules are degraded at random, such that a semilogarithmic plot of isotope remaining in a protein versus time is linear. Thus, we can determine the metabolic half-life of a particular protein. In a rat the average protein has a half-life of 1 or 2 days. ...
... individual molecules are degraded at random, such that a semilogarithmic plot of isotope remaining in a protein versus time is linear. Thus, we can determine the metabolic half-life of a particular protein. In a rat the average protein has a half-life of 1 or 2 days. ...
Moonlighting proteins—an update
... proteins make use of the general methods seen in previously identified moonlighting proteins to switch between functions, although the details differ, for example how interacting with a different protein partner or cofactor results in a conformational change that then affects function. For some of the n ...
... proteins make use of the general methods seen in previously identified moonlighting proteins to switch between functions, although the details differ, for example how interacting with a different protein partner or cofactor results in a conformational change that then affects function. For some of the n ...
Application Note: Using the NanoDrop One to Quantify Protein and
... quantification methods to choose from including gravimetric approaches, colorimetric assays, direct spectrophotometric UV measurements (such as A280), and amino acid analysis. All of these methods have their strengths and weaknesses. Direct spectrophotometric microvolume UV measurements are a popula ...
... quantification methods to choose from including gravimetric approaches, colorimetric assays, direct spectrophotometric UV measurements (such as A280), and amino acid analysis. All of these methods have their strengths and weaknesses. Direct spectrophotometric microvolume UV measurements are a popula ...
Lecture 32: Spectroscopy (continued)
... Example of the isobestic points Since isobestic points occur if and only if there are only two absorbing species, it can be a very useful diagnostic technique, as shown below. ...
... Example of the isobestic points Since isobestic points occur if and only if there are only two absorbing species, it can be a very useful diagnostic technique, as shown below. ...
19-9-ET-V1-S1__preci..
... hence produce minimal denaturation effect on macromolecules. Lower concentration of PEG precipitates higher molecular weight proteins and nucleic acids and vice versa. PEG with different MW is available but PEG with MW more than 4000 are very effective for precipitation. The most important factors f ...
... hence produce minimal denaturation effect on macromolecules. Lower concentration of PEG precipitates higher molecular weight proteins and nucleic acids and vice versa. PEG with different MW is available but PEG with MW more than 4000 are very effective for precipitation. The most important factors f ...
Definition of a RACK1 Interaction Network in Drosophila
... Identification of 37 RACK1 interacting proteins using Data-dependent acquisition In order to define the RACK1 interactome in Drosophila melanogaster, N- or C-terminal FLAG-tagged RACK1 were transiently expressed in Drosophila S2 cells, in mock or virus-infected conditions (Figure 1). A vector expres ...
... Identification of 37 RACK1 interacting proteins using Data-dependent acquisition In order to define the RACK1 interactome in Drosophila melanogaster, N- or C-terminal FLAG-tagged RACK1 were transiently expressed in Drosophila S2 cells, in mock or virus-infected conditions (Figure 1). A vector expres ...
chapter 18 - rci.rutgers.edu
... to alpha-ketoglutarate by specific aminotransferase enzymes (656). The resulting glutamate is then oxidatively deaminated by glutamate DH which can use either NAD+ or NADP+. The ammonia produced is generally incorporated into urea for excretion. Ammonia is toxic if allowed to build up. You should un ...
... to alpha-ketoglutarate by specific aminotransferase enzymes (656). The resulting glutamate is then oxidatively deaminated by glutamate DH which can use either NAD+ or NADP+. The ammonia produced is generally incorporated into urea for excretion. Ammonia is toxic if allowed to build up. You should un ...
Publications
... Binding of the Schiff base metal complexes with the most abundant carrier proteins (serum albumins) have been a subject of interest as such drug–protein binding greatly influences absorption, drug transport, storage, metabolism and excretion properties of typical drugs in vertebrates. Serum albumins ...
... Binding of the Schiff base metal complexes with the most abundant carrier proteins (serum albumins) have been a subject of interest as such drug–protein binding greatly influences absorption, drug transport, storage, metabolism and excretion properties of typical drugs in vertebrates. Serum albumins ...
Macromolecule Review
... 8. Based on your answers to questions 1-7, what simple rule(s) can you use to identify the following macromolecules? Carbohydrates Lipids Proteins Nucleic acids DNA vs. RNA ...
... 8. Based on your answers to questions 1-7, what simple rule(s) can you use to identify the following macromolecules? Carbohydrates Lipids Proteins Nucleic acids DNA vs. RNA ...
The Human Cell Poster Advertisements
... estimated to be many times more—possibly as many as a million*. This is because a single gene might produce multiple variants of a particular protein through, for example, alternative splicing of the messenger RNA. Posttranslational modification of the nascent protein, such as phosphorylation and gl ...
... estimated to be many times more—possibly as many as a million*. This is because a single gene might produce multiple variants of a particular protein through, for example, alternative splicing of the messenger RNA. Posttranslational modification of the nascent protein, such as phosphorylation and gl ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.