Profilin regulates the activity of p42 , a novel Myb
... plasma membrane is clearly only part of profilin’s multifaceted activities. Recently, it was shown that binding of profilin to nuclear actin is a prerequisite for the exportin-dependent transport of actin to the cytoplasm (Stuven et al., 2003). Furthermore, the actin-binding site of profilins is not ...
... plasma membrane is clearly only part of profilin’s multifaceted activities. Recently, it was shown that binding of profilin to nuclear actin is a prerequisite for the exportin-dependent transport of actin to the cytoplasm (Stuven et al., 2003). Furthermore, the actin-binding site of profilins is not ...
A Quaternion-Based Definition of Protein
... • Strings of local helical axes identify actual “helices.” ...
... • Strings of local helical axes identify actual “helices.” ...
IMGT Colliers de Perles: Standardized Sequence
... (TR), major histocompatibility complex (MHC) of human and other vertebrates, and related proteins of the immune system (RPI) of any species. RPI include proteins that belong to the immunoglobulin superfamily (IgSF) and MHC superfamily (MhcSF). IMGT has set up a unique numbering system, which takes i ...
... (TR), major histocompatibility complex (MHC) of human and other vertebrates, and related proteins of the immune system (RPI) of any species. RPI include proteins that belong to the immunoglobulin superfamily (IgSF) and MHC superfamily (MhcSF). IMGT has set up a unique numbering system, which takes i ...
Characterization of Saccharomyces cerevisiae deficient in
... The fact that stretches of identical amino acids are present in PLDs expressed by both plants and yeast suggests that these sequences may represent a consensus sequence for PLDs. Until the regions of the enzyme involved in substrate recognition and catalysis have been identified, and PLDs have been ...
... The fact that stretches of identical amino acids are present in PLDs expressed by both plants and yeast suggests that these sequences may represent a consensus sequence for PLDs. Until the regions of the enzyme involved in substrate recognition and catalysis have been identified, and PLDs have been ...
Chapter 5.9 THE USE OF D-AMINO ACIDS IN PEPTIDE DESIGN
... structures formed by all-L polypeptides adopt a right-handed twist (310-R/αR), while the corresponding Dpolypeptides form helices with a left-handed twist (310-L/αL). Notably, there are small sterically allowed regions, which correspond to positive φ values for L-residues and negative φ values for D ...
... structures formed by all-L polypeptides adopt a right-handed twist (310-R/αR), while the corresponding Dpolypeptides form helices with a left-handed twist (310-L/αL). Notably, there are small sterically allowed regions, which correspond to positive φ values for L-residues and negative φ values for D ...
Microsomes and S9 Prepared from Renal Tissue Yield
... subcellular fractions, pooled samples were subjected to several freeze/thaw cycles, and analyzed for lauric acid 12-hydroxylation, umbelliferone glucuronidation and NADPH-cytochrome c reduction. As shown in Figure 3, renal samples prepared by XenoTech's subcellular fractionation protocol can be repe ...
... subcellular fractions, pooled samples were subjected to several freeze/thaw cycles, and analyzed for lauric acid 12-hydroxylation, umbelliferone glucuronidation and NADPH-cytochrome c reduction. As shown in Figure 3, renal samples prepared by XenoTech's subcellular fractionation protocol can be repe ...
The characterization of Amino Acids and the Purification of Proteins
... • Of these acids, 20 serve as the building blocks of proteins . • Known as the standard, or alpha, amino acids, they comprise serine and tryptophan which are 2 of the 20 that are constructed according to a ...
... • Of these acids, 20 serve as the building blocks of proteins . • Known as the standard, or alpha, amino acids, they comprise serine and tryptophan which are 2 of the 20 that are constructed according to a ...
Studier`s autoinduction media
... fresh overnight culture grown in PA-0.5G. Growth at 37C from a thousand-fold dilution into PASM5052 typically reaches saturation in 14-16 hours. Growth at 20C is much slower and a culture can take 3 days or longer to become induced and reach saturation. Although still testing, we expect that the c ...
... fresh overnight culture grown in PA-0.5G. Growth at 37C from a thousand-fold dilution into PASM5052 typically reaches saturation in 14-16 hours. Growth at 20C is much slower and a culture can take 3 days or longer to become induced and reach saturation. Although still testing, we expect that the c ...
Antibody purification handbook
... each other by disulphide bonds. These disulphide bonds are located in a flexible region of the heavy chain known as the hinge, a region of approximately 12 amino acids that is exposed to enzymatic or chemical cleavage. Each globular region formed by the folding of the polypeptide chains as a result ...
... each other by disulphide bonds. These disulphide bonds are located in a flexible region of the heavy chain known as the hinge, a region of approximately 12 amino acids that is exposed to enzymatic or chemical cleavage. Each globular region formed by the folding of the polypeptide chains as a result ...
Case Study: BPTI
... • The two β-strands forming an anti-parallel β-sheet (Ile18 to Asn24 and Leu29 to Try35). • The three disulfide bonds D1, D2, and D3 (Cys5/55, Cys14/38 and Cys30/51). • The reactive site which binds trypsin or other proteolytic enzymes, located between residues Lys15 and Ala16, near the disulfide bo ...
... • The two β-strands forming an anti-parallel β-sheet (Ile18 to Asn24 and Leu29 to Try35). • The three disulfide bonds D1, D2, and D3 (Cys5/55, Cys14/38 and Cys30/51). • The reactive site which binds trypsin or other proteolytic enzymes, located between residues Lys15 and Ala16, near the disulfide bo ...
Functional analysis of hyperthermophilic endocellulase from
... the C-terminus, and determined the apo-enzyme structure [15]. However, we could not obtain X-ray diffraction data suitable for determination of the structure of the enzyme–substrate ligand complex. Failure to obtain suitable diffraction data might have been caused by the presence of zinc ions tightl ...
... the C-terminus, and determined the apo-enzyme structure [15]. However, we could not obtain X-ray diffraction data suitable for determination of the structure of the enzyme–substrate ligand complex. Failure to obtain suitable diffraction data might have been caused by the presence of zinc ions tightl ...
Neonatal Parenteral Nutrition - UCSF Benioff Children`s Hospital
... Very preterm infants may not tolerate that much dextrose and may even need insulin as an infusion to achieve adequate caloric intake without hyperglycemia. Advance by 1-3 mg/kg/min daily to a maximum of 12 mg/kg/min (up to 15 mg/kg/min in selected cases). Dextrose yields 3.4 kcal/g. Consult reverse ...
... Very preterm infants may not tolerate that much dextrose and may even need insulin as an infusion to achieve adequate caloric intake without hyperglycemia. Advance by 1-3 mg/kg/min daily to a maximum of 12 mg/kg/min (up to 15 mg/kg/min in selected cases). Dextrose yields 3.4 kcal/g. Consult reverse ...
Homology Modelling
... CENTER FOR BIOLOGICAL SEQUENCE ANALYSIS TECHNICAL UNIVERSITY OF DENMARK DTU ...
... CENTER FOR BIOLOGICAL SEQUENCE ANALYSIS TECHNICAL UNIVERSITY OF DENMARK DTU ...
INOTROPIC AGENTS - Dr Ted Williams
... Both block regeneration of reduced form of vitamin K. Termed indirect acting agents SLOW ONSET: already functional coagulation cofactors are not affected by these compounds depletion of circulating Vitamin K-dependent clotting factors must occur via normal protein catabolism ...
... Both block regeneration of reduced form of vitamin K. Termed indirect acting agents SLOW ONSET: already functional coagulation cofactors are not affected by these compounds depletion of circulating Vitamin K-dependent clotting factors must occur via normal protein catabolism ...
JNK1 plays an important part in this process provides an
... archaeal H/ACA RNA and the Cbf5–Nop10 dimer [5]. Moreover, the PUA domain is essential for RNP assembly and activity because (in the presence of substrate RNA) it supports both processes, even when added in trans [6]. By using the coordinates of the TruB–tRNA complex and a previously solved complex ...
... archaeal H/ACA RNA and the Cbf5–Nop10 dimer [5]. Moreover, the PUA domain is essential for RNP assembly and activity because (in the presence of substrate RNA) it supports both processes, even when added in trans [6]. By using the coordinates of the TruB–tRNA complex and a previously solved complex ...
Quantum binding energy features of the T3-785
... peptide T3-785, as well as the details of how they are organized into the three structural zones (Fig. ES4‡), namely N-terminal (amino-acid residues comprising three Pro–Hyp–Gly repetitions), central (sequence Ile–Thr–Gly–Ala–Arg–Gly–Leu–Ala–Gly of human type III collagen) and C-terminal (four Pro–H ...
... peptide T3-785, as well as the details of how they are organized into the three structural zones (Fig. ES4‡), namely N-terminal (amino-acid residues comprising three Pro–Hyp–Gly repetitions), central (sequence Ile–Thr–Gly–Ala–Arg–Gly–Leu–Ala–Gly of human type III collagen) and C-terminal (four Pro–H ...
mammalian hibernation: biochemical adaptation
... being free fatty acid (FFA) anions. Fatty acid anions are transported out of the matrix by UCP1. They are protonated in the acidic intermembrane space, and then neutral FFA-H diffuse across the inner membrane and dissociate again in the more basic pH environment of the matrix. The net effect is that ...
... being free fatty acid (FFA) anions. Fatty acid anions are transported out of the matrix by UCP1. They are protonated in the acidic intermembrane space, and then neutral FFA-H diffuse across the inner membrane and dissociate again in the more basic pH environment of the matrix. The net effect is that ...
Nitrogen Cycle
... o Nitrogen present in the air is in the static form N2 that is it cannot be used by any organism until it is fixed by a process called nitrogen cycle. o Through precipitation, nitrogen enters the soil and surface waters. oWhen it settles down in the soil or water, it converts into two separate atoms ...
... o Nitrogen present in the air is in the static form N2 that is it cannot be used by any organism until it is fixed by a process called nitrogen cycle. o Through precipitation, nitrogen enters the soil and surface waters. oWhen it settles down in the soil or water, it converts into two separate atoms ...
Free tyrosine and tyrosine-rich peptide
... Previously, generation of superoxide anion (O2•-) catalyzed by Cu-binding peptides derived from human prion protein (model sequence for helical Cu-binding motif VNITKQHTVTTTT was most active) in the presence of catecholamines and related aromatic monoamines such as phenylethylamine and tyramine, has ...
... Previously, generation of superoxide anion (O2•-) catalyzed by Cu-binding peptides derived from human prion protein (model sequence for helical Cu-binding motif VNITKQHTVTTTT was most active) in the presence of catecholamines and related aromatic monoamines such as phenylethylamine and tyramine, has ...
RELIC – A bioinformatics server for combinatorial
... motifs for small molecules have been identified and are compiled within databases of sequence motifs such as PROSITE [8], small molecule binding motifs have been characterized for only a small fraction of biological ligands, and even in those cases are far from exhaustive. For instance, although the ...
... motifs for small molecules have been identified and are compiled within databases of sequence motifs such as PROSITE [8], small molecule binding motifs have been characterized for only a small fraction of biological ligands, and even in those cases are far from exhaustive. For instance, although the ...
Cell-Free Phospholipid Biosynthesis by Gene
... synthesis in E. coli entails two acyltransferase enzymes: the glycerol-3-phosphate (G3P) acyltransferase (GPAT) and the lysophosphatidic acid (LPA) acyltransferase (LPAAT) [8,9]. The enzyme GPAT is an integral membrane protein that uses G3P and either acyl-CoA (CoA, coenzyme A) or acyl-ACP (ACP, acy ...
... synthesis in E. coli entails two acyltransferase enzymes: the glycerol-3-phosphate (G3P) acyltransferase (GPAT) and the lysophosphatidic acid (LPA) acyltransferase (LPAAT) [8,9]. The enzyme GPAT is an integral membrane protein that uses G3P and either acyl-CoA (CoA, coenzyme A) or acyl-ACP (ACP, acy ...
Comparative Analysis of Prothrombin Activators
... possessing either group C or D prothrombin activators. The overall domain arrangement in these proteins was highly conserved between all elapids and with the corresponding mammalian clotting factors. The deduced protein sequence for the factor X–like protease precursor, identified in elapids contain ...
... possessing either group C or D prothrombin activators. The overall domain arrangement in these proteins was highly conserved between all elapids and with the corresponding mammalian clotting factors. The deduced protein sequence for the factor X–like protease precursor, identified in elapids contain ...
Gene Section DRAM (damage-regulated autophagy modulator) Atlas of Genetics and Cytogenetics
... DRAM shares no homology with any proteins of known function. DRAM has no known functional domains. Human DRAM shares significant homology with other 6 transmembrane proteins of unknown function, including TMEM77, TMEM150 (TM6P1), and FLJ12993. TM6P1 was cloned by subtractive hybridization as induced ...
... DRAM shares no homology with any proteins of known function. DRAM has no known functional domains. Human DRAM shares significant homology with other 6 transmembrane proteins of unknown function, including TMEM77, TMEM150 (TM6P1), and FLJ12993. TM6P1 was cloned by subtractive hybridization as induced ...
Proteins of Human Milk. I. Identification of Major Components
... about 90% of human colostral immunoglobulins are IgA, about 2% IgG (58-60). The immunoglobulin concentration is very high in colostrum, but declines rapidly in the first few postnatal days as the milk matures (58- 60) . Other milk proteins. Lactoferrin, an iron-binding protein, is found in both bovi ...
... about 90% of human colostral immunoglobulins are IgA, about 2% IgG (58-60). The immunoglobulin concentration is very high in colostrum, but declines rapidly in the first few postnatal days as the milk matures (58- 60) . Other milk proteins. Lactoferrin, an iron-binding protein, is found in both bovi ...
Structure based hypothesis of a mitochondrial
... of Ser-195 to glycine in mtRF1, most likely to accommodate the inserted threonine side chain. It should be noted that this is also one of the identified critical changes between mtRF1 and mtRF1a that we identified. The altered loop conformation that results from the RTinsert seems to completely bloc ...
... of Ser-195 to glycine in mtRF1, most likely to accommodate the inserted threonine side chain. It should be noted that this is also one of the identified critical changes between mtRF1 and mtRF1a that we identified. The altered loop conformation that results from the RTinsert seems to completely bloc ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.