Identification of Bioactive Peptide Sequences from Amaranth
... is limited information about the bioactivity of peptides from amaranth proteins. The objective of this comprehensive review was to determine bioactive peptide sequences in amaranth proteins that may prevent cardiovascular disease, cancer, and diabetes. Amaranth proteins, reported in UniProt database ...
... is limited information about the bioactivity of peptides from amaranth proteins. The objective of this comprehensive review was to determine bioactive peptide sequences in amaranth proteins that may prevent cardiovascular disease, cancer, and diabetes. Amaranth proteins, reported in UniProt database ...
Sixth Southeast Enzyme Conference
... H2O2 by two mechanisms. KatG bears great structural similarity to typical peroxidases like cytochrome c peroxidase. However, it is the sole member of its superfamily with appreciable catalase activity. Contrary to the prevailing paradigm which predicts mutual antagonism between KatG’s two activities ...
... H2O2 by two mechanisms. KatG bears great structural similarity to typical peroxidases like cytochrome c peroxidase. However, it is the sole member of its superfamily with appreciable catalase activity. Contrary to the prevailing paradigm which predicts mutual antagonism between KatG’s two activities ...
The AP-3 adaptor complex is required for vacuolar function
... Subcellular trafficking is required for a multitude of functions in eukaryotic cells. It involves regulation of cargo sorting, vesicle formation, trafficking and fusion processes at multiple levels. Adaptor protein (AP) complexes are key regulators of cargo sorting into vesicles in yeast and mammals ...
... Subcellular trafficking is required for a multitude of functions in eukaryotic cells. It involves regulation of cargo sorting, vesicle formation, trafficking and fusion processes at multiple levels. Adaptor protein (AP) complexes are key regulators of cargo sorting into vesicles in yeast and mammals ...
Optimizing cofactor availability for the production of recombinant
... Co-overexpressions of HEM genes with HRP. HRP overexpression was regulated by PAOX1, co-overexpressions of eGFP and HEM were regulated by PGAP. Bars are average values of HRP production landscapes from microscale cultivations using 1 mM FeSO4-supplemented medium. Error bars are standard deviations f ...
... Co-overexpressions of HEM genes with HRP. HRP overexpression was regulated by PAOX1, co-overexpressions of eGFP and HEM were regulated by PGAP. Bars are average values of HRP production landscapes from microscale cultivations using 1 mM FeSO4-supplemented medium. Error bars are standard deviations f ...
Candida antarctica Anders G. Sandström
... understand that it is Saccharomyces cerevisiae, bakers‟ yeast, which carries out an anaerobic oxidation of carbohydrates to form ethanol. Saccharomyces cerevisiae was used by the Bayer corporation already in the 1930‟s to form a precursor to ephedrine, L-phenylacetylcarbinol via whole-cell biotransf ...
... understand that it is Saccharomyces cerevisiae, bakers‟ yeast, which carries out an anaerobic oxidation of carbohydrates to form ethanol. Saccharomyces cerevisiae was used by the Bayer corporation already in the 1930‟s to form a precursor to ephedrine, L-phenylacetylcarbinol via whole-cell biotransf ...
Coenzyme B 12-Dependent Ribonucleotide Reductase: Evidence
... ABSTRACT: Ribonucleoside triphosphate reductase (RTPR) from Lactobacillus leichmannii catalyzes the conversion of ribonucleotides to 2’-deoxyribonucleotides and requires adenosylcobalamin (AdoCbl) as a cofactor. Recent cloning, sequencing, and expression of this protein [Booker, S., & Stubbe, J. (19 ...
... ABSTRACT: Ribonucleoside triphosphate reductase (RTPR) from Lactobacillus leichmannii catalyzes the conversion of ribonucleotides to 2’-deoxyribonucleotides and requires adenosylcobalamin (AdoCbl) as a cofactor. Recent cloning, sequencing, and expression of this protein [Booker, S., & Stubbe, J. (19 ...
Conceptual Questions C1. Answer: The start codon begins at the
... Initiation: The mRNA, initiator tRNA, and initiation factors associate with the small ribosomal subunit; then the large subunit associates. Elongation: The ribosome moves one codon at a time down the mRNA, adding one amino acid at a time to the growing polypeptide chain. Three sites on the ribosome, ...
... Initiation: The mRNA, initiator tRNA, and initiation factors associate with the small ribosomal subunit; then the large subunit associates. Elongation: The ribosome moves one codon at a time down the mRNA, adding one amino acid at a time to the growing polypeptide chain. Three sites on the ribosome, ...
Evolution of the Aldose Reductase-Related Gecko Eye Lens Protein
... the overexpressed lens version of a gecko housekeeping aldose reductase, because B-crystallin does not display the characteristic aldose reductase activity (see below). It thus may have arisen by gene duplication of a functional aldose reductase gene. If so, this duplication likely has been a relat ...
... the overexpressed lens version of a gecko housekeeping aldose reductase, because B-crystallin does not display the characteristic aldose reductase activity (see below). It thus may have arisen by gene duplication of a functional aldose reductase gene. If so, this duplication likely has been a relat ...
Cobalt Biology Discussion - 1-29-15
... binding to an organic agent Mg2+ (Ca2+) < Mn2+ < Fe2+ < Co2+ < Ni2+ < Cu2+ > Zn2+, but becomes much more stable in the trivalent form Al3+ << Cr3+ < Fe3+ Mn3+ < Co3+ (Williams, 1981). These trivalent ions, except Al3+ can form very strong complexes to N/O- side chain groups of proteins, but this is ...
... binding to an organic agent Mg2+ (Ca2+) < Mn2+ < Fe2+ < Co2+ < Ni2+ < Cu2+ > Zn2+, but becomes much more stable in the trivalent form Al3+ << Cr3+ < Fe3+ Mn3+ < Co3+ (Williams, 1981). These trivalent ions, except Al3+ can form very strong complexes to N/O- side chain groups of proteins, but this is ...
Protein composition and function of red and white skeletal - AJP-Cell
... white skeletal muscle have very similar protein composition because nearly all the protein spots are yellow or the same between mitochondrial types. Proteins listed in Fig. 1, A and D, are those that have been identified many times by our laboratory (4, 5, 8, 20, 27, 41, 45) as well as by Kim et al. ...
... white skeletal muscle have very similar protein composition because nearly all the protein spots are yellow or the same between mitochondrial types. Proteins listed in Fig. 1, A and D, are those that have been identified many times by our laboratory (4, 5, 8, 20, 27, 41, 45) as well as by Kim et al. ...
A: _____/18
... transition state, enzyme will increase the concentration of the transition state, thus increasing the rate of the reaction (6 pts). The reduction in energy of the transition state can be due to two factors: enthalpy and entropy. enthalpy: The enzyme forms some kind of interaction in the transition s ...
... transition state, enzyme will increase the concentration of the transition state, thus increasing the rate of the reaction (6 pts). The reduction in energy of the transition state can be due to two factors: enthalpy and entropy. enthalpy: The enzyme forms some kind of interaction in the transition s ...
Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is
... (Lamzin et al., 1992) and the formate-binding site from Arg-285 are indicated. The sequences were highly conserved between barley and potato in contrast to the sequences at the N terminus, which displayed very low sequence homology. Southern Hybridization Analysis The copy number of Fdh in barley wa ...
... (Lamzin et al., 1992) and the formate-binding site from Arg-285 are indicated. The sequences were highly conserved between barley and potato in contrast to the sequences at the N terminus, which displayed very low sequence homology. Southern Hybridization Analysis The copy number of Fdh in barley wa ...
Document
... Lack of trace metals becomes limiting for growth in P-0.5G without added metals. Iron, manganese and cobalt were the most effective in relieving this limitation. A concentration of 0.1x trace metals mixture is sufficient to support maximal growth in P-0.5G. Growth in ZYP medium is not limited by lac ...
... Lack of trace metals becomes limiting for growth in P-0.5G without added metals. Iron, manganese and cobalt were the most effective in relieving this limitation. A concentration of 0.1x trace metals mixture is sufficient to support maximal growth in P-0.5G. Growth in ZYP medium is not limited by lac ...
The contribution of the Trp/Met/Phe residues to physical interactions
... of Lys residues by CBP/p300–p53 interactions [7–10]. On the other hand, proteins physically interacting with p53 can also regulate p53 function without chemical modifications. The combination of functional biological approaches and structural biological studies has provided much information about th ...
... of Lys residues by CBP/p300–p53 interactions [7–10]. On the other hand, proteins physically interacting with p53 can also regulate p53 function without chemical modifications. The combination of functional biological approaches and structural biological studies has provided much information about th ...
The standard procedure starts with a set of sequences
... such an alignment. This number is called similarity score or alignment score and serves to evaluate the significance of a potential motif occurrence. Like most similarity search techniques, a protein database search with a profile returns a sorted list of potential matches ranked by a quality score. ...
... such an alignment. This number is called similarity score or alignment score and serves to evaluate the significance of a potential motif occurrence. Like most similarity search techniques, a protein database search with a profile returns a sorted list of potential matches ranked by a quality score. ...
Sequence Specific Modeling of E. coli Cell-Free Protein
... (17, 22) and protein structures (GEM-PRO) (23, 24). These expansions have greatly increased the scope of questions these models can explore. Thus, constraint based methods are powerful tools to estimate the performance of metabolic networks with very few adjustable parameters. However, constraint ba ...
... (17, 22) and protein structures (GEM-PRO) (23, 24). These expansions have greatly increased the scope of questions these models can explore. Thus, constraint based methods are powerful tools to estimate the performance of metabolic networks with very few adjustable parameters. However, constraint ba ...
Biomarker discovery for psychiatric disorders
... established and optimized. Mouse synaptosome proteome profiling was carried out to create a reference map for quantitative proteomics experiments. Furthermore, the quantitative proteomics platform based on metabolic labeling of the HAB/NAB/LAB mouse model with the isotope was established, and the ...
... established and optimized. Mouse synaptosome proteome profiling was carried out to create a reference map for quantitative proteomics experiments. Furthermore, the quantitative proteomics platform based on metabolic labeling of the HAB/NAB/LAB mouse model with the isotope was established, and the ...
Globins in Nonvertebrate Species: Dispersal by Horizontal Gene
... John Czeluzniak, 5 Morris Goodman, 5 Mark Blaxter,ll and Serge Vinogradofl *Department of Biochemistry, University of Antwerp; TDepartment of Morphology, Systematics and Ecology, University of Ghent; *Department of Radiology and Enrico Fermi Institute, University of Chicago; SDepartment of Anatomy, ...
... John Czeluzniak, 5 Morris Goodman, 5 Mark Blaxter,ll and Serge Vinogradofl *Department of Biochemistry, University of Antwerp; TDepartment of Morphology, Systematics and Ecology, University of Ghent; *Department of Radiology and Enrico Fermi Institute, University of Chicago; SDepartment of Anatomy, ...
AP Biology 2007-2008 Chemistry of Carbon Building
... animal cell membranes precursor of all other steroids ...
... animal cell membranes precursor of all other steroids ...
Proteolytic processing in the secretory pathway of Aspergillus niger
... extracellular environment they will reach their target after a journey through the secretory pathway (Fig. 1). The endoplasmic reticulum is the entry point into the secretory pathway. The synthesized precursor proteins contain a hydrophobic prepeptide which mediates their translocation into the ER. ...
... extracellular environment they will reach their target after a journey through the secretory pathway (Fig. 1). The endoplasmic reticulum is the entry point into the secretory pathway. The synthesized precursor proteins contain a hydrophobic prepeptide which mediates their translocation into the ER. ...
view file - S
... common stay-green phenotype in several plant species, (ii) sgr1 mutants display a type C nonfunctional (cosmetic) staygreen phenotype, which affects Chl degradation, but not other aspects of senescence (Thomas and Howarth, 2000), and (iii) rice SGR1 physically interacts with light-harvesting complex ...
... common stay-green phenotype in several plant species, (ii) sgr1 mutants display a type C nonfunctional (cosmetic) staygreen phenotype, which affects Chl degradation, but not other aspects of senescence (Thomas and Howarth, 2000), and (iii) rice SGR1 physically interacts with light-harvesting complex ...
How to move an amphipathic molecule across a lipid
... coupling helices contained within the long intracellular loops linking TM helices interact with the NBD of the opposite subunit in an arrangement termed ‘domain swapping’ [47]. Interestingly, ABCD proteins have a significant number of conserved hydrophilic residues in predicted transmembrane helices ...
... coupling helices contained within the long intracellular loops linking TM helices interact with the NBD of the opposite subunit in an arrangement termed ‘domain swapping’ [47]. Interestingly, ABCD proteins have a significant number of conserved hydrophilic residues in predicted transmembrane helices ...
Purified Hexameric Epstein-Barr Virus-Encoded BARF1 Protein for Measuring Anti-BARF1 Antibody
... Amstelveen, Netherlands). Subsequently, the sBARF1 B95-8 sequence (amino acid [aa] 21 to aa 221) was cloned into the pHTA donor vector, adding a 6-His tag C terminally (BARF1-His vector). In addition, the sBARF1 sequence (aa 21 to aa 221) was cloned into the pGEX 4T-3 vector (Amersham Biosciences, G ...
... Amstelveen, Netherlands). Subsequently, the sBARF1 B95-8 sequence (amino acid [aa] 21 to aa 221) was cloned into the pHTA donor vector, adding a 6-His tag C terminally (BARF1-His vector). In addition, the sBARF1 sequence (aa 21 to aa 221) was cloned into the pGEX 4T-3 vector (Amersham Biosciences, G ...
Immunoblot Detection of Proteins That Contain Cysteine
... pathological conditions such as inflammation and ischemiareperfusion. The generation of ROS also appears to be required for many normal cellular functions, including transduction of cell surface receptor signaling. Thiols of cysteine residues in proteins are the most common targets of ROS in cells. ...
... pathological conditions such as inflammation and ischemiareperfusion. The generation of ROS also appears to be required for many normal cellular functions, including transduction of cell surface receptor signaling. Thiols of cysteine residues in proteins are the most common targets of ROS in cells. ...
Protein–protein interaction
Protein–protein interactions (PPIs) refer to physical contacts established between two or more proteins as a result of biochemical events and/or electrostatic forces.In fact, proteins are vital macromolecules, at both cellular and systemic levels, but they rarely act alone. Diverse essential molecular processes within a cell are carried out by molecular machines that are built from a large number of protein components organized by their PPIs. Indeed, these interactions are at the core of the entire interactomics system of any living cell and so, unsurprisingly, aberrant PPIs are on the basis of multiple diseases, such as Creutzfeld-Jacob, Alzheimer's disease, and cancer.PPIs have been studied from different perspectives: biochemistry, quantum chemistry, molecular dynamics, signal transduction, among others. All this information enables the creation of large protein interaction networks – similar to metabolic or genetic/epigenetic networks – that empower the current knowledge on biochemical cascades and disease pathogenesis, as well as provide putative new therapeutic targets.