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Non-cell-autonomous regulation of root hair patterning genes by
Non-cell-autonomous regulation of root hair patterning genes by

... Root hair cells (H-cells) develop from cell files over the cleft of two underlying cortical cells, whereas all other cells develop into non-root hair cells (N-cells) (Dolan et al., 1993; Dolan et al., 1994; Galway et al., 1994). The genetic dissection of the underlying pathway has enabled the isolat ...
The importance of alternative splicing in the drug discovery process
The importance of alternative splicing in the drug discovery process

... Antisense oligonucleotides can be designed to inhibit study the function of the gene. However, it turned out that abnormal, disease-causing splicing. Such an approach was not all the gene is shut down and a residual binding activused to correct an aberrant splicing of the cystic fibrosis ity remains ...
genetic studies of the human complement c4 region in mhc class iii
genetic studies of the human complement c4 region in mhc class iii

... need to be recognized and removed, an action provided by the immune system. The immune system is an organization of molecules, cells and tissues with a specific function to protect from infectious disease. Defense against microbes is mediated by the immediate innate responses and the later responses ...
Wnt Signaling and an APC-Related Gene Specify Endoderm in
Wnt Signaling and an APC-Related Gene Specify Endoderm in

... pharyngeal tissue and the E daughters divide prematurely. Thus, apr-1 and wrm-1 both appear to play a role in endoderm specification, while wrm-1 may have additional functions required for proper morphogenesis. We tested 11 other genes that showed sequence similarity to components of the Wnt/WG-sign ...
Chapter 13
Chapter 13

... • In the beginning of telophase I, each half of the cell has a haploid set of chromosomes; each chromosome still consists of two sister chromatids ...
Efficient Delivery of Dharmacon SMARTpool® siRNA
Efficient Delivery of Dharmacon SMARTpool® siRNA

... to identify gene function and elucidate biological pathways. Successful siRNA experiments involving knockdown of individual genes or collections of gene targets require efficient delivery of highly functional and specific siRNA molecules into appropriate cells. While lipid-mediated transfection is a ...
- Wiley Online Library
- Wiley Online Library

... The discovery that the ubiquitin protein ligase called the Anaphase Promoting Complex or Cyclosome (APC/C) responsible for destroying mitotic cyclins at the metaphase-toanaphase transition is also required for sister chromatid separation provided an impetus to the search for cohesion proteins using ...
File - jj-sct
File - jj-sct

... inheritance has its physical basis in the behavior of chromosomes: Scientific inquiry  The first solid evidence associating a specific gene with a specific chromosome came in the early 20th century from the work of Thomas Hunt Morgan  These early experiments provided convincing evidence that the c ...
Principal Components Analysis
Principal Components Analysis

... sense of this data is to reduce its dimensionality. Several data decomposition techniques are available for this purpose: Principal Components Analysis (PCA) is among these techniques that reduces the data into two dimensions. ...
Role of PTEN/PI3K pathway in endothelial cells
Role of PTEN/PI3K pathway in endothelial cells

... cytokines, chemokines and hormones to their receptors, as well as antigen receptor engagement. PIP3 generated by PI3K activation in turn activates PKB (protein kinase B)/ Akt, a serine/threonine kinase involved in anti-apoptosis, proliferation and oncogenesis. By dephosphorylating the D3 position of ...
Quantification of Protein Levels in Single Living
Quantification of Protein Levels in Single Living

... high protein expression. For cell biologists, accurate measurement of protein concentration ensures the accurate interpretation of gene function in certain phenotypes. The ability to quantify proteins in single cells at any given spatial and temporal moment and then associate the measured protein co ...
EIN4 and ERS2 Are Members of the Putative Ethylene Receptor
EIN4 and ERS2 Are Members of the Putative Ethylene Receptor

... yeast osmolarity sensing pathway (Maeda et al., 1994). Alternately, the ethylene receptors could interact directly with CTR1 (Clark et al., 1998). EIN3 acts downstream of ETR1 and CTR1. It encodes a novel nuclear-localized protein and probably is involved in transcriptional regulation (Chao et al., ...
meiosis - juan
meiosis - juan

... • In the beginning of telophase I, each half of the cell has a haploid set of chromosomes; each chromosome still consists of two sister chromatids ...
Comparative Analysis of Structural Diversity and
Comparative Analysis of Structural Diversity and

... cleavage sites. Results of the targeting prediction programs are shown in supplementary table S2, Supplementary Material online. We considered a sequence to be mitochondrially targeted if two or more of the prediction programs predicted mitochondrial targeting. In addition to mitochondrial targeting ...
An organism containing a normal chromosome complement and
An organism containing a normal chromosome complement and

... Other chromosome abnormalities ...
French 2003 - University of Washington
French 2003 - University of Washington

... Anterior is upper left. The dorsal side of the egg chamber is marked by the dorsal appendages, which extend anteriorly from the main body of the egg. (B) A stage-14 twk egg chamber. The arrows indicate the mutant dorsal appendages. While two appendages initially formed with proper spacing, these app ...
E.coli
E.coli

... 1. The analysis of genome organization and the identification of genes, particularly in organisms with large genome sizes (human DNA is 3  109 bp, for example) is difficult to use plasmid and bacteriophage  vectors, since the relatively small size capacity of these vectors for cloned DNA means tha ...
Regulatory Genes Controlling MPG7 Expression
Regulatory Genes Controlling MPG7 Expression

... In filamentous fungi, nitrogen utilization pathways are activated by wide-domain regulators (Arst and Cove, 1973). In A. nidulans and N. crassa, these wide-domain regulators are members of the GATA family of DNA binding proteins and have been shown to be transcriptional activators encoded by the a r ...
The Genetics of Beta-galactosidase
The Genetics of Beta-galactosidase

... several steps, from transcription to translation to the final folding and processing of the protein. Regulation can occur (and has been shown to occur) at any of these steps. Beta-Galactosidase and the lac Operon in the Molecular Biology Lab Why is the lac operon so important to molecular biologists ...
Factors Affecting synonymous codon Usage Bias in chloroplast
Factors Affecting synonymous codon Usage Bias in chloroplast

... Nevertheless, Morton found that the asymmetry of two strands of the chloroplast genome from Euglena gracilis was the major factor contributing to codon bias.12 In addition, it was considered that context dependent mutation played some roles in shaping codon usage of the chloroplast genomes of grass ...
Biology EOCEP Review
Biology EOCEP Review

... As cells divide and grow they become specialized in their structure and function. What is this process called? ...
Notch activity induces Nodal expression and mediates the
Notch activity induces Nodal expression and mediates the

The Arabidopsis WAVY GROWTH 2 Protein Modulates Root
The Arabidopsis WAVY GROWTH 2 Protein Modulates Root

... WAV2 locus causes an abnormality in the root bending in response to the touch stimulus at the root tip but that it does not cause severe defects in the root growth. Takahashi et al. (2002) reported that hydrotropic curvature was increased in the roots of wav2-1. We tested whether this mutant also sh ...
Molecular Evaluation of Vitamin D3 Receptor Agonists
Molecular Evaluation of Vitamin D3 Receptor Agonists

... than the three other VDR agonists (EC50 value of 0.2 nM), but 50 times higher concentrations (EC50 value of 10 nM) were needed to stabilize the VDR in solution. Stabilization of VDR-RXR-VDRE complexes In order to con®rm the DNA-dependent sensitivity of the four VDR agonists, ligand-dependent gel shi ...
Specification of floral organs in Arabidopsis
Specification of floral organs in Arabidopsis

... of A function in the original ABC model), it also suppresses the expression of the B function genes AP3 and PI (Krogan et al., 2012). AP2 is therefore, in all probability, not involved in promoting petal development (for which B function gene activity is essential) as predicted by the original ABC m ...
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Polycomb Group Proteins and Cancer

The Polycomb-group proteins (PcGs) are a family of proteins that use epigenetic mechanisms to maintain or repress expression of their target genes. They were originally discovered in Drosophila (fruit flies), though they've been shown to be conserved in many species due to their vital roles in embryonic development. These proteins' ability to alter gene expression has made them targets of investigation for research groups seeking to understand disease pathology and oncology.
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