A versatile toolbox for PCR-based tagging of yeast genes: new
... protein complex purification (Gavin et al., 2002; Ho et al., 2002), as well as for single gene-function analysis. The strategy requires: (a) a pair of primers that contain within their 5 region sequences of homology to the genomic target location; and (b) PCR-cassettes (also termed ‘modules’) that ...
... protein complex purification (Gavin et al., 2002; Ho et al., 2002), as well as for single gene-function analysis. The strategy requires: (a) a pair of primers that contain within their 5 region sequences of homology to the genomic target location; and (b) PCR-cassettes (also termed ‘modules’) that ...
Developmental and genetic disorders in
... et al., 1997). Such disorders can be treated successfully by replacement therapy with either GnRH or a combination of follicle stimulating hormone (FSH) and luteinizing hormone (LH) (Behre et al., 1997). Other disorders in spermatogenesis fail to respond to endocrine therapy and require intervention ...
... et al., 1997). Such disorders can be treated successfully by replacement therapy with either GnRH or a combination of follicle stimulating hormone (FSH) and luteinizing hormone (LH) (Behre et al., 1997). Other disorders in spermatogenesis fail to respond to endocrine therapy and require intervention ...
Gene Section LPP (lipoma preferred partner) Atlas of Genetics and Cytogenetics
... Because of their structural features (many proteinprotein interaction domains) and their characteristic to shuttle between the nucleus and the cytoplasm, LPP and its family members (see below) have been proposed to be scaffolding proteins involved in signal transduction from sites of cell adhesion t ...
... Because of their structural features (many proteinprotein interaction domains) and their characteristic to shuttle between the nucleus and the cytoplasm, LPP and its family members (see below) have been proposed to be scaffolding proteins involved in signal transduction from sites of cell adhesion t ...
Role of virulence factors, cell components and adhesion in
... mononuclear cells we cocultivated human THP-1 acute monocytic leukemia cells and murine J774A.1 professional macrophages with different H. pylori wild-type strains and mutants. We have shown that H. pylori-mediated iNOS induction in J774A.1 is independent of established virulence factors but depende ...
... mononuclear cells we cocultivated human THP-1 acute monocytic leukemia cells and murine J774A.1 professional macrophages with different H. pylori wild-type strains and mutants. We have shown that H. pylori-mediated iNOS induction in J774A.1 is independent of established virulence factors but depende ...
Leukaemia Section T-lineage acute lymphoblastic leukemia (T-ALL) Atlas of Genetics and Cytogenetics
... conventional cytogenetics, this abnormality represents about 17% of all T-ALL cases (Table 1). By molecular cytogenetics studies, the incidence of TRA@/TRD@ rearrangements is about 24% of all T-ALL cases. t(1;14)(p32;q11.2). This translocation is observed in about 3% of T-ALL. The TAL1(TCL5/SCL) gen ...
... conventional cytogenetics, this abnormality represents about 17% of all T-ALL cases (Table 1). By molecular cytogenetics studies, the incidence of TRA@/TRD@ rearrangements is about 24% of all T-ALL cases. t(1;14)(p32;q11.2). This translocation is observed in about 3% of T-ALL. The TAL1(TCL5/SCL) gen ...
Cloning and Functional Studies of a Splice Variant of
... Four lesions were divided in two and incubated in DMEM/F12 enriched with 30 mg/mL of human albumin (Biovitrum AB, Stockholm, Sweden) for 6 h with 1 mM atRA (Sigma-Aldrich, St. Louis, MO, USA) or vehicle (DMSO). These studies were ...
... Four lesions were divided in two and incubated in DMEM/F12 enriched with 30 mg/mL of human albumin (Biovitrum AB, Stockholm, Sweden) for 6 h with 1 mM atRA (Sigma-Aldrich, St. Louis, MO, USA) or vehicle (DMSO). These studies were ...
Cockayne Syndrome group B protein interacts
... into a large non-coding telomere repeat-containing RNA (5), referred to as TERRA, which is implicated in maintaining the integrity of telomere heterochromatin (5,6). Disruption of the shelterin complex or the telomere heterochromatic state can lead to induction of telomere abnormalities, including t ...
... into a large non-coding telomere repeat-containing RNA (5), referred to as TERRA, which is implicated in maintaining the integrity of telomere heterochromatin (5,6). Disruption of the shelterin complex or the telomere heterochromatic state can lead to induction of telomere abnormalities, including t ...
Chapter 13 PPT
... • In the beginning of telophase I, each half of the cell has a haploid set of chromosomes; each chromosome still consists of two sister chromatids ...
... • In the beginning of telophase I, each half of the cell has a haploid set of chromosomes; each chromosome still consists of two sister chromatids ...
Full Text - G3: Genes | Genomes | Genetics
... insertion (meIs1) expressing GFP-LacI using the germline-specific pie-1 promoter (Praitis et al. 2001; Yuen et al. 2011) and a large array of lacO repeats integrated into a single genomic locus on chromosome III (meIs4). In this strain, the event that integrated the lacO array onto chromosome III als ...
... insertion (meIs1) expressing GFP-LacI using the germline-specific pie-1 promoter (Praitis et al. 2001; Yuen et al. 2011) and a large array of lacO repeats integrated into a single genomic locus on chromosome III (meIs4). In this strain, the event that integrated the lacO array onto chromosome III als ...
Operon review
... The regulation of this operon is an example of __________________ control. positive ...
... The regulation of this operon is an example of __________________ control. positive ...
13_lecture_meiosis
... • In the beginning of telophase I, each half of the cell has a haploid set of chromosomes; each chromosome still consists of two sister chromatids ...
... • In the beginning of telophase I, each half of the cell has a haploid set of chromosomes; each chromosome still consists of two sister chromatids ...
Curr. Microbiol. 42
... cry7 (Fig. 1A). A necessary condition for defining a new gene is either different interval(s) among the five conserved blocks, as found in cry8 (Fig 1B) and in other cry genes [28, 29], or homology variation in conserved blocks. This method can be practical for a distinct cry group or for several gr ...
... cry7 (Fig. 1A). A necessary condition for defining a new gene is either different interval(s) among the five conserved blocks, as found in cry8 (Fig 1B) and in other cry genes [28, 29], or homology variation in conserved blocks. This method can be practical for a distinct cry group or for several gr ...
The Differential Killing of Genes by Inversions in Prokaryotic Genomes
... seems important to analyze gene translocations regarding the asymmetrical organization of bacterial chromosomes and the leading and lagging roles of DNA strands. There is still no consensus about which strand— leading or lagging—is synthesized with higher fidelity. We have found that the divergence ...
... seems important to analyze gene translocations regarding the asymmetrical organization of bacterial chromosomes and the leading and lagging roles of DNA strands. There is still no consensus about which strand— leading or lagging—is synthesized with higher fidelity. We have found that the divergence ...
Cutting Edge: DNA Polymerases and Are Dispensable for Ig Gene
... Pol has been considered a good candidate for the elusive Ig gene mutase according to several criteria (12, 13): 1) its very strong homology with Tdt, a strictly lymphoid-specific enzyme whose contribution is crucial to diversify the third complementarity-determining region of Ig and TCR genes duri ...
... Pol has been considered a good candidate for the elusive Ig gene mutase according to several criteria (12, 13): 1) its very strong homology with Tdt, a strictly lymphoid-specific enzyme whose contribution is crucial to diversify the third complementarity-determining region of Ig and TCR genes duri ...
two loci affecting b cell responses to b cell
... B cells to IFN-~, is therefore not present, or is much weaker, in DBA/2Ha macrophages. To characterize the genetic basis for the unresponsiveness of DBA/2Ha B cells to BMF, F1 progeny were produced by crossing nonresponsive DBA/2Ha and responsive C57BL/6J parents (Fig. 2). When challenged with any o ...
... B cells to IFN-~, is therefore not present, or is much weaker, in DBA/2Ha macrophages. To characterize the genetic basis for the unresponsiveness of DBA/2Ha B cells to BMF, F1 progeny were produced by crossing nonresponsive DBA/2Ha and responsive C57BL/6J parents (Fig. 2). When challenged with any o ...
Genome-Wide Analysis of In Vivo Binding of the Master Regulator
... growth-phase dependent manner [7]. A pivotal question to address is how primary environmental signals are relayed to global carbon and nitrogen control and further to the control of development and antibiotic production. The elucidation of the S. coelicolor genome sequence— the first from an antibio ...
... growth-phase dependent manner [7]. A pivotal question to address is how primary environmental signals are relayed to global carbon and nitrogen control and further to the control of development and antibiotic production. The elucidation of the S. coelicolor genome sequence— the first from an antibio ...
Gene prediction and Genome Annotation
... • New genomes require new approaches or at least require re-optimilisation of software • Isoforms (NGS data) • “New genes” , eg. lncRNAs, anti-sense RNAs, … • New “organisation” of genes – Overlapping genes (transcript, CDS) – Nested genes ...
... • New genomes require new approaches or at least require re-optimilisation of software • Isoforms (NGS data) • “New genes” , eg. lncRNAs, anti-sense RNAs, … • New “organisation” of genes – Overlapping genes (transcript, CDS) – Nested genes ...
Autosomal and X-chromosome imprinting
... between TISn and T28H (shown in italics in Fig. 2), and studies with these may further define the exact region subject to imprinting. Table 1 summarizes the frequencies with which the hypo- and hyperkinetic classes have been detected in studies with a series of different chromosome 2 translocations. ...
... between TISn and T28H (shown in italics in Fig. 2), and studies with these may further define the exact region subject to imprinting. Table 1 summarizes the frequencies with which the hypo- and hyperkinetic classes have been detected in studies with a series of different chromosome 2 translocations. ...
Homologous Recombination Generates T-Loop
... to cancer and aging. Telomere attrition represents a tumor suppressor pathway that limits the replicative potential of potential cancer cells. The gradual loss of telomeric DNA with each round of DNA replication depletes the telomere reserve and leads to a growth arrest that is accompanied by senesc ...
... to cancer and aging. Telomere attrition represents a tumor suppressor pathway that limits the replicative potential of potential cancer cells. The gradual loss of telomeric DNA with each round of DNA replication depletes the telomere reserve and leads to a growth arrest that is accompanied by senesc ...
Editorial - Ontario Association on Developmental Disabilities
... pregnancy, then fetal chromosomes can be studied in cells from amniotic fluid or from a chorionic villus biopsy. Down syndrome can also be diagnosed by studying chromosomes shortly after birth. In opposition to commonly held beliefs, individuals with Down syndrome vary considerable in their appearan ...
... pregnancy, then fetal chromosomes can be studied in cells from amniotic fluid or from a chorionic villus biopsy. Down syndrome can also be diagnosed by studying chromosomes shortly after birth. In opposition to commonly held beliefs, individuals with Down syndrome vary considerable in their appearan ...
Promega Notes 49: Firefly Luciferase Engineered for Improved
... Normally, in the firefly light organ, luciferase is located in specialized peroxisomes of the photocytic cells. When expressed in foreign hosts, a conserved translocation signal within the enzyme structure causes it to accumulate in peroxisomes and glyoxisomes. In moderate to high levels of expressi ...
... Normally, in the firefly light organ, luciferase is located in specialized peroxisomes of the photocytic cells. When expressed in foreign hosts, a conserved translocation signal within the enzyme structure causes it to accumulate in peroxisomes and glyoxisomes. In moderate to high levels of expressi ...
Incomplete penetrance of MHC susceptibility genes
... such traits prospectively in persons presumed to be homozygous, heterozygous or non-carrying for susceptibility genes by determining trait expression in homozygotes, heterozygotes or non-carriers of trait-associated conserved extended MHC haplotypes. The method provides direct estimates of apparent ...
... such traits prospectively in persons presumed to be homozygous, heterozygous or non-carrying for susceptibility genes by determining trait expression in homozygotes, heterozygotes or non-carriers of trait-associated conserved extended MHC haplotypes. The method provides direct estimates of apparent ...
msb201035-sup
... (Fisher exact test, p<0.05). The fact that the timing of Tinman binding is not highly correlation with the timing of its target genes’ expression most likely reflects the importance of co-operativity with other TFs for Tinman function, as previously reported (e.g. HsiuHsiang Lee and Manfred Frasch, ...
... (Fisher exact test, p<0.05). The fact that the timing of Tinman binding is not highly correlation with the timing of its target genes’ expression most likely reflects the importance of co-operativity with other TFs for Tinman function, as previously reported (e.g. HsiuHsiang Lee and Manfred Frasch, ...