Supplementary Materials and Methods (doc 73K)
... polystyrene flasks and cultured in DMEM medium (Euroclone, Pero, Milan, Italy) with the addition of 10% fetal bovine serum (FBS; Sigma, St. Louis, MO, USA). Primary MM plasma cells were isolated from non-adherent BMMCs using magnetic cell sorting with anti-CD138-conjugated microbeads (MACS; Miltenyi ...
... polystyrene flasks and cultured in DMEM medium (Euroclone, Pero, Milan, Italy) with the addition of 10% fetal bovine serum (FBS; Sigma, St. Louis, MO, USA). Primary MM plasma cells were isolated from non-adherent BMMCs using magnetic cell sorting with anti-CD138-conjugated microbeads (MACS; Miltenyi ...
Stem cells and their role in regenerative processes of the liver
... human livers are c-kit positive, suggests that the c-kit/stem cell factor receptor-ligand system may also play a role. Other factors responsible for homing of circulating cells to the liver as well ...
... human livers are c-kit positive, suggests that the c-kit/stem cell factor receptor-ligand system may also play a role. Other factors responsible for homing of circulating cells to the liver as well ...
Embryology and Stem Cells
... ball of 64 cells called a morula. Human embryology is just like the embryology of all other animals until the morula stage. Cells in the morula stage are totipotent stem cells. ...
... ball of 64 cells called a morula. Human embryology is just like the embryology of all other animals until the morula stage. Cells in the morula stage are totipotent stem cells. ...
Stem Cell Mobilization And Procurement
... – Rich source of haemopoeitic stem and progenitor cells. – Contains more naive T-cells than bone marrow which allows for a greater mis- match without increased risk of GVHD – No risk to donors – Decreased risk of infection ...
... – Rich source of haemopoeitic stem and progenitor cells. – Contains more naive T-cells than bone marrow which allows for a greater mis- match without increased risk of GVHD – No risk to donors – Decreased risk of infection ...
PHS 398/2590 (Rev. 06/09), Biographical Sketch Format
... significant exchanges between all of our COBREs and our diverse and talented pool of investigators continue to benefit from the varied core services that are offered and the excellent mentoring that we as a united group provide. I have extensive experience in studies of stem cell biology and have ev ...
... significant exchanges between all of our COBREs and our diverse and talented pool of investigators continue to benefit from the varied core services that are offered and the excellent mentoring that we as a united group provide. I have extensive experience in studies of stem cell biology and have ev ...
Biological characterization and in vitro culture of human
... been considered dissimilar with important biological differences in the identity and behavior of stem cells, recent studies from our laboratory and others recently challenged this concept. Expect for the existence of type Apale and Adark spermatogonia, and that Ada ...
... been considered dissimilar with important biological differences in the identity and behavior of stem cells, recent studies from our laboratory and others recently challenged this concept. Expect for the existence of type Apale and Adark spermatogonia, and that Ada ...
Human dendritic cell differentiation from monocytes
... the PPAR (peroxisome proliferator activated receptor)-gamma stimulation can give rise to LCs in vitro starting from CD14+ monocytes, as occurs with CD133+ haematopoietic precursors (1). Circulating CD14+ monocytes have been isolated by immunomagnetic separation and cultured with GM-CSF, IL-4, TNF-α ...
... the PPAR (peroxisome proliferator activated receptor)-gamma stimulation can give rise to LCs in vitro starting from CD14+ monocytes, as occurs with CD133+ haematopoietic precursors (1). Circulating CD14+ monocytes have been isolated by immunomagnetic separation and cultured with GM-CSF, IL-4, TNF-α ...
Will stem cells be a key resource for research in IBD in the future in
... Spheroids are enriched for stem cells in 50% L-WRN CM ...
... Spheroids are enriched for stem cells in 50% L-WRN CM ...
After School Physiology review 2013
... In the lungs-between alveoli (air sacs) and capillaries (small blood vessels) • What parts are involved, in order? Nose, pharynx, larynx, trachea, bronchi, lungs • What does the diaphragm do? Contracts to bring air in, relaxes for air to go out • What is the epiglottis? Flap that prevents food from ...
... In the lungs-between alveoli (air sacs) and capillaries (small blood vessels) • What parts are involved, in order? Nose, pharynx, larynx, trachea, bronchi, lungs • What does the diaphragm do? Contracts to bring air in, relaxes for air to go out • What is the epiglottis? Flap that prevents food from ...
CELL SPECIALIZATION - Biology with Miss Amy
... of the ratio between surface area and volume; as cell size increases, the SA:V ratio decreases meaning it becomes more difficult for substances to ...
... of the ratio between surface area and volume; as cell size increases, the SA:V ratio decreases meaning it becomes more difficult for substances to ...
Columbia-2010
... (from GFP-transgenic mice) are co-cultured in 384-well format and individual shRNAs delivered in arrayed format to each component. We have developed high content microscopy to quantify cell number, morphology and cobblestoning capacity (a measure of self-renewal ability) in these co-cultures. A para ...
... (from GFP-transgenic mice) are co-cultured in 384-well format and individual shRNAs delivered in arrayed format to each component. We have developed high content microscopy to quantify cell number, morphology and cobblestoning capacity (a measure of self-renewal ability) in these co-cultures. A para ...
Test Study Guide-cell processes_ homeostasis2
... SHORT ANSWER: Know the four things that cells need to maintain homeostasis: obtain and use energy, make new cells, exchange materials, and eliminate wastes Know that cells in multicellular organisms work together to maintain homeostasis for the entire organism. SHORT ANSWER: Know the main tran ...
... SHORT ANSWER: Know the four things that cells need to maintain homeostasis: obtain and use energy, make new cells, exchange materials, and eliminate wastes Know that cells in multicellular organisms work together to maintain homeostasis for the entire organism. SHORT ANSWER: Know the main tran ...
I`m Bigger Than You
... made up of muscle tissue, but also contains connective and nerve tissue. However, each of these individual types of tissues has its own primary function, which differs from the basic function of the heart. Without the assistance of the cells in different organs, an entire organism is unable to stay ...
... made up of muscle tissue, but also contains connective and nerve tissue. However, each of these individual types of tissues has its own primary function, which differs from the basic function of the heart. Without the assistance of the cells in different organs, an entire organism is unable to stay ...
video summaries: cells
... The%movement%of%water from%an%area%of% high%concentra2on%to%low%concentra2on% ...
... The%movement%of%water from%an%area%of% high%concentra2on%to%low%concentra2on% ...
Carcinogenesis – The Development of Cancer
... The cells of the cancer, at least at its beginning, must have been derived from a single cell that went wrong. The cell must have independent ability to divide continuously and out of control. The cell must be able to separate from its neighbouring cells and move to a new location where a new mass o ...
... The cells of the cancer, at least at its beginning, must have been derived from a single cell that went wrong. The cell must have independent ability to divide continuously and out of control. The cell must be able to separate from its neighbouring cells and move to a new location where a new mass o ...
Biology Cell revision
... • Use iodine solution for plant cells (e.g. onion cells) • Use methylene blue for animal cells (e.g. human cheek cells) 4. Lower a cover slip carefully over the cells – this helps to prevent air bubbles. 5. Place prepared slide onto microscope. 6. View the slide using the low power lens first moving ...
... • Use iodine solution for plant cells (e.g. onion cells) • Use methylene blue for animal cells (e.g. human cheek cells) 4. Lower a cover slip carefully over the cells – this helps to prevent air bubbles. 5. Place prepared slide onto microscope. 6. View the slide using the low power lens first moving ...
5.5 Lecture slides
... • Organs are groups of tissues that perform a specific or related function. • Organ systems are groups of organs that carry out similar functions. ...
... • Organs are groups of tissues that perform a specific or related function. • Organ systems are groups of organs that carry out similar functions. ...
Biology Test
... 1. The basic units or building blocks of all life are cells. 2. Cells are responsible for growth, repair and reproduction. 3. Benign tumours divide uncontrollably and destroy the surrounding tissue. 4. Blood is considered to be a tissue. 5. Carcinogens prevent cancer. 6. All cells divide at the same ...
... 1. The basic units or building blocks of all life are cells. 2. Cells are responsible for growth, repair and reproduction. 3. Benign tumours divide uncontrollably and destroy the surrounding tissue. 4. Blood is considered to be a tissue. 5. Carcinogens prevent cancer. 6. All cells divide at the same ...
Poietics™ hematopoietic progenitor growth medium
... please refer to the product numbers and descriptions listed above. For a complete listing of all Poietics™ products, refer to the Lonza website or our current catalog. To obtain a catalog, additional information or scientific support you may contact Lonza by web, e-mail, telephone, fax or mail. ...
... please refer to the product numbers and descriptions listed above. For a complete listing of all Poietics™ products, refer to the Lonza website or our current catalog. To obtain a catalog, additional information or scientific support you may contact Lonza by web, e-mail, telephone, fax or mail. ...
B cells
... Other secondary lymphoid organs Less organized Peyer’s patches in small intestine contain M cells collect intestinal material transfer to lymphoid tissue below mucosa MALT – mucosal-associated lymphoid tissue includes Peyer’s patches provides “mucosal” immunity prevents organisms from entering muco ...
... Other secondary lymphoid organs Less organized Peyer’s patches in small intestine contain M cells collect intestinal material transfer to lymphoid tissue below mucosa MALT – mucosal-associated lymphoid tissue includes Peyer’s patches provides “mucosal” immunity prevents organisms from entering muco ...
DNA damage (Comet Assay) as biomarker of Cd exposure in
... Copper oxide (CuO) is one of the most widely used nanoparticle applications in consumer products. They are extensively used in microelectronics, cosmetics and catalysts. In the present study, the DNA damaging potential of CuO-NPs in the marine eastern mussel Mytilus trossulus was evaluated and compa ...
... Copper oxide (CuO) is one of the most widely used nanoparticle applications in consumer products. They are extensively used in microelectronics, cosmetics and catalysts. In the present study, the DNA damaging potential of CuO-NPs in the marine eastern mussel Mytilus trossulus was evaluated and compa ...
Slide 1 - Catawba County Schools
... Which of the following statements about hand-washing is true? • A) You don’t need to wash your hands if you wear gloves • B) You must use an antimicrobial soap to be effective • C) Wash hands for a minimum of 5 seconds • D) Wash hands immediately after touching body fluids or secretions ...
... Which of the following statements about hand-washing is true? • A) You don’t need to wash your hands if you wear gloves • B) You must use an antimicrobial soap to be effective • C) Wash hands for a minimum of 5 seconds • D) Wash hands immediately after touching body fluids or secretions ...
Hematopoiesis: Stem Cells
... transformation. Unlike normal haematopoiesis, where signalling pathways that have been proposed to regulate self-renewal are tightly regulated (top), during transformation of stem cells, the same mechanisms may be dysregulated to allow uncontrolled self-renewal (middle). Furthermore, if the transfor ...
... transformation. Unlike normal haematopoiesis, where signalling pathways that have been proposed to regulate self-renewal are tightly regulated (top), during transformation of stem cells, the same mechanisms may be dysregulated to allow uncontrolled self-renewal (middle). Furthermore, if the transfor ...
Name - Valhalla High School
... Finding shelter, avoiding predators, and obtaining food are most closely related to the ability of an animal to a. increase the rate of cell division c. use structures adapted for movement b. transport carbon dioxide to cells d. excrete waste products to cells 5. _______ If a human system fails to f ...
... Finding shelter, avoiding predators, and obtaining food are most closely related to the ability of an animal to a. increase the rate of cell division c. use structures adapted for movement b. transport carbon dioxide to cells d. excrete waste products to cells 5. _______ If a human system fails to f ...
Hematopoietic stem cell
Hematopoietic stem cells (HSCs) are the blood cells that give rise to all the other blood cells and are derived from mesoderm. They are located in the red bone marrow, which is contained in the core of most bones.They give rise to both the myeloid and lymphoid lineages of blood cells. (Myeloid cells include monocytes, macrophages, neutrophils, basophils, eosinophils, erythrocytes, dendritic cells, and megakaryocytes or platelets. Lymphoid cells include T cells, B cells, and natural killer cells.) The definition of hematopoietic stem cells has changed in the last two decades. The hematopoietic tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent progenitors. HSCs constitute 1:10.000 of cells in myeloid tissue.HSCs are a heterogeneous population. The third category consists of the balanced (Bala) HSC, whose L/M ratio is between 3 and 10. Only the myeloid-biased and -balanced HSCs have durable self-renewal properties. In addition, serial transplantation experiments have shown that each subtype preferentially re-creates its blood cell type distribution, suggesting an inherited epigenetic program for each subtype.HSC studies through much of the past half century have led to a much deeper understanding. More recent advances have resulted in the use of HSC transplants in the treatment of cancers and other immune system disorders.