to TCR Ligand Potency Within MHC Molecule or Antigenic Peptide
... Vergelli, Nick Ling, Paul Conlon, Henry F. McFarland, Richard Houghten and Roland Martin ...
... Vergelli, Nick Ling, Paul Conlon, Henry F. McFarland, Richard Houghten and Roland Martin ...
Analyzing the antibody against H-Y antigen in hematopoietic cell
... HLA-DQ (HLA-DQA1, and HLA-DQB1), and HLA-DR (HLA-DRA), as well as four βchains (however only three are possible per person–HLA-DRB1, DRB3, DRB4, DRB5). Similar to MHC class I genes, the class II genes are highly polymorphic.8 The function of multiple MHC subunits and allele polymorphism is to allow ...
... HLA-DQ (HLA-DQA1, and HLA-DQB1), and HLA-DR (HLA-DRA), as well as four βchains (however only three are possible per person–HLA-DRB1, DRB3, DRB4, DRB5). Similar to MHC class I genes, the class II genes are highly polymorphic.8 The function of multiple MHC subunits and allele polymorphism is to allow ...
Full text - The American Association of Immunologists
... Diseases from 1968 to 1970. Desiring a return to academic life, he accepted an appointment as chair of the Department of Comparative Pathology at Harvard Medical School in 1970. In 1980, he left Harvard to serve as president and CEO of the Dana-Farber Cancer Institute, a position he held until his r ...
... Diseases from 1968 to 1970. Desiring a return to academic life, he accepted an appointment as chair of the Department of Comparative Pathology at Harvard Medical School in 1970. In 1980, he left Harvard to serve as president and CEO of the Dana-Farber Cancer Institute, a position he held until his r ...
Pro5® Pentamer Applications
... • CD1d tetramers can be used to isolate antigen-specific CD1drestricted NKT cell populations • Results of CD1d staining can be combined with other techniques, (e.g. ELISPOT) to gain a full picture of an NKT cell specific response ...
... • CD1d tetramers can be used to isolate antigen-specific CD1drestricted NKT cell populations • Results of CD1d staining can be combined with other techniques, (e.g. ELISPOT) to gain a full picture of an NKT cell specific response ...
Oct 10, 15 Chapter 6 - Signaling through immune system receptors
... Certain T and B lymphocytes exhibit only a very limited diversity of receptors that are encoded by a few common gene rearrangements. These lymphocytes, intraepithelial cells and B-1 cells, behave like intermediates between adaptive and innate immunity. Characteristics of innate immunity: act im ...
... Certain T and B lymphocytes exhibit only a very limited diversity of receptors that are encoded by a few common gene rearrangements. These lymphocytes, intraepithelial cells and B-1 cells, behave like intermediates between adaptive and innate immunity. Characteristics of innate immunity: act im ...
CSA Biopharm - Moodle Lille 2
... DCs.DCs take up the protein and process it HLA molecules present epitopes from the protein on the DC surface. Cells are lyzedand HLApeptide molecules are then recovered in an immune affinity step and analyzed by sequencing mass spectrometry. Peptides are recovered from the HLA ...
... DCs.DCs take up the protein and process it HLA molecules present epitopes from the protein on the DC surface. Cells are lyzedand HLApeptide molecules are then recovered in an immune affinity step and analyzed by sequencing mass spectrometry. Peptides are recovered from the HLA ...
Transient Reversions in MHC
... The MHC:peptide tetramer is made from recombinant Mamu-A*01-p11C complexes, bound to streptavidin via biotin, labeled with ...
... The MHC:peptide tetramer is made from recombinant Mamu-A*01-p11C complexes, bound to streptavidin via biotin, labeled with ...
Peptide-Binding Specificity Molecule, Defines a New Supertype of
... discovery of promiscuous peptides, which bind to multiple HLA II molecules, and greatly enhances the potential for the use of epitope-based vaccines (10 –15). Supertypes were initially described for the class I molecules (16 –18) and only attributed to HLA-DR molecules for the class II molecules (6, ...
... discovery of promiscuous peptides, which bind to multiple HLA II molecules, and greatly enhances the potential for the use of epitope-based vaccines (10 –15). Supertypes were initially described for the class I molecules (16 –18) and only attributed to HLA-DR molecules for the class II molecules (6, ...
The danger model in deciphering autoimmunity
... cell death, characterized by the presence or absence of certain secreted or membrane-bound antigens that bring about different immunological responses [23]. By means of one or more ill-defined ligand(s), apoptotic cells under some circumstances appear to elicit an anti-inflammatory response in conta ...
... cell death, characterized by the presence or absence of certain secreted or membrane-bound antigens that bring about different immunological responses [23]. By means of one or more ill-defined ligand(s), apoptotic cells under some circumstances appear to elicit an anti-inflammatory response in conta ...
Exosomes with major histocompatibility complex class II and
... ABSTRACT: Exosomes are 30–100 nm diameter vesicles formed by inward budding of endosomal compartments and are produced by several cell types, including T-cells, Bcells and dendritic cells (DC)s. Exosomes from DCs express major histocompatibility complexes (MHC) class I and II, and co-stimulatory mol ...
... ABSTRACT: Exosomes are 30–100 nm diameter vesicles formed by inward budding of endosomal compartments and are produced by several cell types, including T-cells, Bcells and dendritic cells (DC)s. Exosomes from DCs express major histocompatibility complexes (MHC) class I and II, and co-stimulatory mol ...
by a Novel Mass Spectrometric Technique Cysteinylated Cysteine
... homologue required 1500-fold more peptide than did recognition of the nonoxidized Y peptide, regardless of whether the latter was cysteinylated (Fig. 4). These observations suppport the conclusion that the sequence of the A1-HY epitope is IVDC*LTEMY and is derived from the DFFRY gene. We performed p ...
... homologue required 1500-fold more peptide than did recognition of the nonoxidized Y peptide, regardless of whether the latter was cysteinylated (Fig. 4). These observations suppport the conclusion that the sequence of the A1-HY epitope is IVDC*LTEMY and is derived from the DFFRY gene. We performed p ...
Peptide Repertoire Class I Molecule Q10 Binds a Classical The
... The biological properties of the nonclassical class I MHC molecules secreted into blood and tissue fluids are not currently understood. To address this issue, we studied the murine Q10 molecule, one of the most abundant, soluble class Ib molecules. Mass spectrometry analyses of hybrid Q10 polypeptid ...
... The biological properties of the nonclassical class I MHC molecules secreted into blood and tissue fluids are not currently understood. To address this issue, we studied the murine Q10 molecule, one of the most abundant, soluble class Ib molecules. Mass spectrometry analyses of hybrid Q10 polypeptid ...
during Epstein-Barr Virus Reactivation Transcription by Lytic
... HCMV interferes with MHC class II transcription and CIITA activation. Other strategies for the down-modulation MHC class II surface expression by HCMV are promoting the proteasome-mediated degradation of DR-␣ and DM-␣ molecules by glycoprotein US2 (18, 19). In addition, US3 protein of HCMV competes ...
... HCMV interferes with MHC class II transcription and CIITA activation. Other strategies for the down-modulation MHC class II surface expression by HCMV are promoting the proteasome-mediated degradation of DR-␣ and DM-␣ molecules by glycoprotein US2 (18, 19). In addition, US3 protein of HCMV competes ...
The Disadvantage Being Homozygous
... • Originally detected as blood groups present on white blood cells (Human Leuk(c)ocyte Antigens) but present on almost all nucleated cells and platelets. • More polymorphic than red blood groups: ABO system: 4 possible combinations (A,B,AB, O) HLA system: >1 million combinations (linkage disequilibr ...
... • Originally detected as blood groups present on white blood cells (Human Leuk(c)ocyte Antigens) but present on almost all nucleated cells and platelets. • More polymorphic than red blood groups: ABO system: 4 possible combinations (A,B,AB, O) HLA system: >1 million combinations (linkage disequilibr ...
Reprint - Institute of Biochemistry - Goethe
... secretion of cytokines, which mediate both humoral (antibody dependent) and cell-mediated immunity. MHC I molecules, on the other hand, present peptides from viruses, intracellularly replicating bacteria, or from tumor-specific proteins. CD8+ T lymphocytes recognize the complex of MHC I molecules an ...
... secretion of cytokines, which mediate both humoral (antibody dependent) and cell-mediated immunity. MHC I molecules, on the other hand, present peptides from viruses, intracellularly replicating bacteria, or from tumor-specific proteins. CD8+ T lymphocytes recognize the complex of MHC I molecules an ...
Atypical MHC class II-expressing antigen
... ‘immunogenicity’ (REF. 145), and crucial work by McDevitt, Sela and Humphrey146–148 showed that immunogenicity required ‘immune response’ genes, which mapped to the MHC locus. Zinkernagel and Doherty149 first demonstrated that the presentation of viral antigens was MHC restricted and multiple labora ...
... ‘immunogenicity’ (REF. 145), and crucial work by McDevitt, Sela and Humphrey146–148 showed that immunogenicity required ‘immune response’ genes, which mapped to the MHC locus. Zinkernagel and Doherty149 first demonstrated that the presentation of viral antigens was MHC restricted and multiple labora ...
pH stability of HLA-DR4 complexes with antigenic peptides .
... helper T cells. Consistent with this mechanism for antigen presentation, the peptide fragments that are most effective at eliciting an immune response (immunodominant peptides) generally form complexes with MHC II that are stable for hours at both mildly acidic (endosomal) and neutral (cell surface) ...
... helper T cells. Consistent with this mechanism for antigen presentation, the peptide fragments that are most effective at eliciting an immune response (immunodominant peptides) generally form complexes with MHC II that are stable for hours at both mildly acidic (endosomal) and neutral (cell surface) ...
Excess of Deleterious Mutations around HLA
... frequencies of variants residing on the same haplotype as the positively selected variant (Maynard Smith and Haigh 1974; Fay and Wu 2000; Chun and Fay 2011), and theoretical analysis and empirical data suggest that hitchhiking events can also elevate the frequency of deleterious variants (Chun and F ...
... frequencies of variants residing on the same haplotype as the positively selected variant (Maynard Smith and Haigh 1974; Fay and Wu 2000; Chun and Fay 2011), and theoretical analysis and empirical data suggest that hitchhiking events can also elevate the frequency of deleterious variants (Chun and F ...
T cell
... • A single B or T cell has about 100,000 or these receptors. • The variable region at the tip of each antigen receptor chain (antigen binding site) forms this diversity. • The sequence of a.a. in these regions varies from cell to cell, thus it is estimated that each individual has as many as 1 milli ...
... • A single B or T cell has about 100,000 or these receptors. • The variable region at the tip of each antigen receptor chain (antigen binding site) forms this diversity. • The sequence of a.a. in these regions varies from cell to cell, thus it is estimated that each individual has as many as 1 milli ...
Increased Generation of HIV-1 gp120-Reactive Encoding the Chemokine CCL3
... target HIV-1 surface envelope glycoprotein (Env) gp120 to APC, could increase the immune responses towards the antigen. To protect against HIV-1 and development of AIDS, antibodies that can neutralize a broad number of virus variants, and CD8+ T cells that can eradicate virus-infected cells, are lik ...
... target HIV-1 surface envelope glycoprotein (Env) gp120 to APC, could increase the immune responses towards the antigen. To protect against HIV-1 and development of AIDS, antibodies that can neutralize a broad number of virus variants, and CD8+ T cells that can eradicate virus-infected cells, are lik ...
lymphatic - Ruhr-Universität Bochum
... Topic 3: Immune response to infection (Prof. Bufe) - 22.06.16 1. Phases of infection (Janeway 430, 11.1); Role of innate immune response for adaptive response (Janeway 432; 11.2); Cytokines and different T-cell subsets in response to different pathogens (Janeway 434-439; 11.3-11.5) ...
... Topic 3: Immune response to infection (Prof. Bufe) - 22.06.16 1. Phases of infection (Janeway 430, 11.1); Role of innate immune response for adaptive response (Janeway 432; 11.2); Cytokines and different T-cell subsets in response to different pathogens (Janeway 434-439; 11.3-11.5) ...
Efficient Presentation of Phagocytosed Cellular Fragments on the
... Apoptosis was induced by exposure to UV light. The surface of most cells became annexin V–positive after 8–10 h. Antibodies. The Y-Ae hybridoma was provided by Dr. C. Janeway, Jr. (Yale University, New Haven, CT) and grown serum-free. The IgG2b antibody was purified on protein A–Sepharose, biotinyla ...
... Apoptosis was induced by exposure to UV light. The surface of most cells became annexin V–positive after 8–10 h. Antibodies. The Y-Ae hybridoma was provided by Dr. C. Janeway, Jr. (Yale University, New Haven, CT) and grown serum-free. The IgG2b antibody was purified on protein A–Sepharose, biotinyla ...
Role of Endoplasmic Reticulum Aminopeptidases in Health and
... risk of preeclampsia, a heritable pregnancy specific disorder characterized by new-onset hypertension and proteinuria [46,47]. Of note, ERAP2 expression was previously found altered in first trimester placentas of women prone to develop preeclampsia [48]. 4.2. Bacterial and Viral Infections The firs ...
... risk of preeclampsia, a heritable pregnancy specific disorder characterized by new-onset hypertension and proteinuria [46,47]. Of note, ERAP2 expression was previously found altered in first trimester placentas of women prone to develop preeclampsia [48]. 4.2. Bacterial and Viral Infections The firs ...
Major histocompatibility complex
The major histocompatibility complex (MHC) is a set of cell surface molecules encoded by a large gene family which controls a major part of the immune system in all vertebrates. The major function of major histocompatibility complexes is to bind to peptide fragments derived from pathogens and display them on the cell surface for recognition by the appropriate T-cells. MHC molecules mediate interactions of leukocytes, also called white blood cells (WBCs), which are immune cells, with other leukocytes or with body cells. The MHC determines compatibility of donors for organ transplant, as well as one's susceptibility to an autoimmune disease via crossreacting immunization. In humans, the MHC is also called the human leukocyte antigen (HLA).In a cell, protein molecules of the host's own phenotype or of other biologic entities are continually synthesized and degraded. Each MHC molecule on the cell surface displays a molecular fraction of a protein, called epitope. The presented antigen can be either 'self' or 'nonself', thus preventing an organism`s immune system targeting its own cells. In its entirety, the MHC population is like a meter indicating the balance of proteins within the cell.The MHC gene family is divided into three subgroups: class I, class II, and class III. Class I MHC molecules have β2 subunits so can only be recognised by CD8 co-receptors. Class II MHC molecules have no β2 subunits so can be recognised by CD4 co-receptors. In this way MHC molecules chaperones which type of lymphocytes may bind to the given antigen with high affinity, since different lymphocytes express different TCR co-receptors. Diversity of antigen presentation, mediated by MHC classes I and II, is attained in at least three ways: (1) an organism's MHC repertoire is polygenic (via multiple, interacting genes); (2) MHC expression is codominant (from both sets of inherited alleles); (3) MHC gene variants are highly polymorphic (diversely varying from organism to organism within a species). Major histocompatibility complex and sexual selection has been observed in male mice making mate choices of females with different MHCs and thus demonstrating sexual selection.