Pinto, A. K., A. M. Jamieson, D. H. Raulet, and A. B. Hill. 2007. The role of NKG2D signaling in inhibition of cytotoxic T-lymphocyte lysis by the Murine cytomegalovirus immunoevasin m152/gp40. J. Viro 81:12564-12571 .
... primed cytotoxic CD8 T lymphocytes (CTL) to kill virus-infected cells. Of these three, the impact of m152/gp40 on CTL lysis appears greater than would be expected based on its impact on cell surface major histocompatibility complex (MHC) class I. In addition to MHC class I, m152/gp40 also downregula ...
... primed cytotoxic CD8 T lymphocytes (CTL) to kill virus-infected cells. Of these three, the impact of m152/gp40 on CTL lysis appears greater than would be expected based on its impact on cell surface major histocompatibility complex (MHC) class I. In addition to MHC class I, m152/gp40 also downregula ...
Curriculum Vitae
... 45. Wang P, Sidney J, Dow C, Mothé B, Sette A, Peters B. A systematic assessment of MHC class II peptide binding predictions and evaluation of a consensus approach. PLoS Comput Biol. 2008 Apr 4;4(4):e1000048. PMCID: PMC2267221 46. Assarsson E, Greenbaum JA, Sundström M, Schaffer L, Hammond JA, Pasqu ...
... 45. Wang P, Sidney J, Dow C, Mothé B, Sette A, Peters B. A systematic assessment of MHC class II peptide binding predictions and evaluation of a consensus approach. PLoS Comput Biol. 2008 Apr 4;4(4):e1000048. PMCID: PMC2267221 46. Assarsson E, Greenbaum JA, Sundström M, Schaffer L, Hammond JA, Pasqu ...
Expression of CD1d in human scalp skin and hair follicles: hair cycle
... CD1c isotypes; and group II, which includes CD1d. Only the group II CD1d isotypes are preserved in humans, mice, rats, rabbits, and monkeys.1 6 Sequence similarity is substantially higher for the same isotype from different species than for different isotypes within the same species,5 suggesting tha ...
... CD1c isotypes; and group II, which includes CD1d. Only the group II CD1d isotypes are preserved in humans, mice, rats, rabbits, and monkeys.1 6 Sequence similarity is substantially higher for the same isotype from different species than for different isotypes within the same species,5 suggesting tha ...
Seminar Winter Semester 2002/2003
... • Important to study what parts of a protein that binds to MHC molecules. • MHC I binds peptides with 8-10 aa • MHC II bind peptides with 12-25 aa • Potentials of peptide vaccines • Prediction of peptides is important!!!! ...
... • Important to study what parts of a protein that binds to MHC molecules. • MHC I binds peptides with 8-10 aa • MHC II bind peptides with 12-25 aa • Potentials of peptide vaccines • Prediction of peptides is important!!!! ...
Document
... vaccine antigens such as tetanus toxoid, diphtheria toxoid, pertussis toxoid at doses which are not maximal for that animal model or minimal threshold doses is recommended. Diphtheria toxoid has been found particularly useful, as it is a poor immunogen. ...
... vaccine antigens such as tetanus toxoid, diphtheria toxoid, pertussis toxoid at doses which are not maximal for that animal model or minimal threshold doses is recommended. Diphtheria toxoid has been found particularly useful, as it is a poor immunogen. ...
Peptide Vaccine: Progress and Challenges
... 3. Induction of Protective T-Cell and B-Cell Mediated Immunity by Peptide Vaccines A successful vaccine must induce a strong and long memory humoral and cellular immune response, but more importantly, protect against the disease being targeted. Therefore, it is important to evaluate whether the pept ...
... 3. Induction of Protective T-Cell and B-Cell Mediated Immunity by Peptide Vaccines A successful vaccine must induce a strong and long memory humoral and cellular immune response, but more importantly, protect against the disease being targeted. Therefore, it is important to evaluate whether the pept ...
IFN-γ + CD4 T Cells
... The HLA type of donors was performed by low resolution PCR with sequence-specific primers (PCR-SSP). All presenting HLA-DR-homozygous L-BCL were chosen to conform at least to the donor’s major serological, and usually to molecular, type. The affinity of the presenting MHC class II molecule for pepti ...
... The HLA type of donors was performed by low resolution PCR with sequence-specific primers (PCR-SSP). All presenting HLA-DR-homozygous L-BCL were chosen to conform at least to the donor’s major serological, and usually to molecular, type. The affinity of the presenting MHC class II molecule for pepti ...
Identification of novel CTL epitopes of CMV-pp65
... CMV disease, indicating that T-cell immunity plays an important role in controlling CMV infection. Thus, immunologic monitoring of T-cell immunity against CMV is crucial to evaluate the status of immunocompromised patients. Identification of the CTL epitopes derived from CMV is very valuable not onl ...
... CMV disease, indicating that T-cell immunity plays an important role in controlling CMV infection. Thus, immunologic monitoring of T-cell immunity against CMV is crucial to evaluate the status of immunocompromised patients. Identification of the CTL epitopes derived from CMV is very valuable not onl ...
Mechanisms involved in regulation of MHC class I molecules in
... species to the environment. These molecules are not only crucial for adaptive and innate immune responses against microorganisms and cancer cells but also play an important role in reproduction process and development of embryo during the preimplantation period. In the present study, we use murine e ...
... species to the environment. These molecules are not only crucial for adaptive and innate immune responses against microorganisms and cancer cells but also play an important role in reproduction process and development of embryo during the preimplantation period. In the present study, we use murine e ...
Immunogenic Consensus Sequence T Helper Epitopes for a Pan
... cystic fibrosis patients. Since BPM, BM and BC are all intracellular bacteria, they are excellent targets for T cell-based vaccines. However, the sheer volume of available genomic data requires the aid of immunoinformatics for vaccine design. Using EpiMatrix, ClustiMer and EpiAssembler, a set of imm ...
... cystic fibrosis patients. Since BPM, BM and BC are all intracellular bacteria, they are excellent targets for T cell-based vaccines. However, the sheer volume of available genomic data requires the aid of immunoinformatics for vaccine design. Using EpiMatrix, ClustiMer and EpiAssembler, a set of imm ...
Human-specific evolution of killer cell immunoglobulin
... initial characterization using antibody-based serological assays that distinguish different antigens. The approximately 4.8 Mb HLA complex on chromosome 6 contains many immune system genes and is the most highly polymorphic segment of the human genome [11,12]. Of the six expressed HLA class I genes, ...
... initial characterization using antibody-based serological assays that distinguish different antigens. The approximately 4.8 Mb HLA complex on chromosome 6 contains many immune system genes and is the most highly polymorphic segment of the human genome [11,12]. Of the six expressed HLA class I genes, ...
HYBRID SKELETAL MUSCLE FIBRES: A RARE OR COMMON
... used interchangeably to define fibres that co-express more than one MHC isoform. However, there is now compelling evidence to suggest that this meaning of the term ‘hybrid fibres’ is highly inadequate because it does not apply to fibres displaying patterns of molecular heterogeneity with respect to ...
... used interchangeably to define fibres that co-express more than one MHC isoform. However, there is now compelling evidence to suggest that this meaning of the term ‘hybrid fibres’ is highly inadequate because it does not apply to fibres displaying patterns of molecular heterogeneity with respect to ...
CD4+ T Cells Targeting Dominant and Cryptic Epitopes from
... anthrax infection, it has lately become clear that LF may represent a major target not only for antibody responses, but also T cell immunity in naturally exposed individuals. Our previous work has shown that epitopes identified from domains II and IV of LF dominate a Th1 associated IFNγ response, and ...
... anthrax infection, it has lately become clear that LF may represent a major target not only for antibody responses, but also T cell immunity in naturally exposed individuals. Our previous work has shown that epitopes identified from domains II and IV of LF dominate a Th1 associated IFNγ response, and ...
ELISA technique
... combine with the very antigen that triggered its production (specific affinity) ...
... combine with the very antigen that triggered its production (specific affinity) ...
Induction of Protective Antitumor Immunity through Attenuation of ERAAP Function
... peptides, many of which are generated as N-terminally extended precursors following partial hydrolysis of substrates by cytosolic proteases, including the proteasome (1, 2). These are then transported into the endoplasmic reticulum by the TAP. A key final step of the pathway involves aminopeptidases ...
... peptides, many of which are generated as N-terminally extended precursors following partial hydrolysis of substrates by cytosolic proteases, including the proteasome (1, 2). These are then transported into the endoplasmic reticulum by the TAP. A key final step of the pathway involves aminopeptidases ...
HLA-A*02 AND ITS PROGNOSTIC TRAITS IN CANCER.
... ovarian cancer (EOC) to determine HLA-A, -B, -Cw and -DRB1. The results showed an unusual representation of HLA alleles in EOC compared to healthy Swedish bone-marrow donors where HLA-A*02 was overrepresented, also HLA-A*02 homozygotes were two-fold higher among EOC patients. The frequency of HLA-A* ...
... ovarian cancer (EOC) to determine HLA-A, -B, -Cw and -DRB1. The results showed an unusual representation of HLA alleles in EOC compared to healthy Swedish bone-marrow donors where HLA-A*02 was overrepresented, also HLA-A*02 homozygotes were two-fold higher among EOC patients. The frequency of HLA-A* ...
Primary and Secondary Immune Response: Lectuer-9
... 4. Size and Number of doses 5. Multiple antigens 6. Adjuvant 7. Immunosuppressive agent Cellular Immune Response: The term cell rnediated immunity refers to the specific immune responses that do not involve antibodies, induction of cell mediated immune response (CMI) consists of specifically sensiti ...
... 4. Size and Number of doses 5. Multiple antigens 6. Adjuvant 7. Immunosuppressive agent Cellular Immune Response: The term cell rnediated immunity refers to the specific immune responses that do not involve antibodies, induction of cell mediated immune response (CMI) consists of specifically sensiti ...
BIOGRAPHICAL SKETCH Santambrogio, Laura
... “Empty Class II MHC on dendritic cells and microglia” Principal Investigator: Laura Santambrogio (100% effort) Agency: NIH R01 AI48833 ($ 1,555,000) Period: 01/29/2001-3/31/2008 The long-term objective of this research is to characterize the physiological role and relevance in the immunological resp ...
... “Empty Class II MHC on dendritic cells and microglia” Principal Investigator: Laura Santambrogio (100% effort) Agency: NIH R01 AI48833 ($ 1,555,000) Period: 01/29/2001-3/31/2008 The long-term objective of this research is to characterize the physiological role and relevance in the immunological resp ...
What is the T-cell receptor (TCR)? (abridged
... The α and β proteins are composed of 3 or 4, respectively protein sub units. The unabridged version of this content describes how it is possible to have different combinations of these sub units. This generates a diverse set of T-cells to recognize a variety of foreign things (microbes, allergen ...
... The α and β proteins are composed of 3 or 4, respectively protein sub units. The unabridged version of this content describes how it is possible to have different combinations of these sub units. This generates a diverse set of T-cells to recognize a variety of foreign things (microbes, allergen ...
containing lysosomes in coeliac disease
... Coeliac disease is triggered by ingestion of wheat gliadin and is probably immune mediated. There is evidence by light microscopy that expression of class II major histocompatibility complex (MHC) molecules is increased in the small intestinal epithelium of patients with untreated coeliac disease an ...
... Coeliac disease is triggered by ingestion of wheat gliadin and is probably immune mediated. There is evidence by light microscopy that expression of class II major histocompatibility complex (MHC) molecules is increased in the small intestinal epithelium of patients with untreated coeliac disease an ...
antigens
... enter the body by inhalation, ingestion or injection. these are taken by the APCs and degraded into small peptides. APCs then present them to helper T cells by using MHC type II molecules. ...
... enter the body by inhalation, ingestion or injection. these are taken by the APCs and degraded into small peptides. APCs then present them to helper T cells by using MHC type II molecules. ...
Cytotoxic T-Lymphocyte–Defined Human Minor
... HLA-A2 and 80% do not express HLA-B44, most recipients would not be eligible for therapy targeting any of these four minor H antigens.31 Moreover, even for donor/recipient pairs expressing HLA-A2, the clinical use of HA-2 and HA-5 as targets for GVL therapy is limited because HA-2 and HA-5 are expre ...
... HLA-A2 and 80% do not express HLA-B44, most recipients would not be eligible for therapy targeting any of these four minor H antigens.31 Moreover, even for donor/recipient pairs expressing HLA-A2, the clinical use of HA-2 and HA-5 as targets for GVL therapy is limited because HA-2 and HA-5 are expre ...
Mutations Defining Functional Regions of the Superantigen
... SEB bound to MHC class II molecules, and did not block T cell recognition of SEB bound to D R (data not shown). ELISA for SER The amount of SEB in preparations was determined in an ELISA. Microtiter wells were coated overnight with a solution of 6 #g/ml natural SEB (Sigma Chemical Co., St. Louis, MO ...
... SEB bound to MHC class II molecules, and did not block T cell recognition of SEB bound to D R (data not shown). ELISA for SER The amount of SEB in preparations was determined in an ELISA. Microtiter wells were coated overnight with a solution of 6 #g/ml natural SEB (Sigma Chemical Co., St. Louis, MO ...
antigen-antibody reaction
... But when two antigens are bridge by two antibodies, the binding will be strong. This phenomenon of giving extra- strength to the antigen-antibody complex by the binding of two antibodies to two antigen molecules is called bonus effect. ...
... But when two antigens are bridge by two antibodies, the binding will be strong. This phenomenon of giving extra- strength to the antigen-antibody complex by the binding of two antibodies to two antigen molecules is called bonus effect. ...
Major histocompatibility complex
The major histocompatibility complex (MHC) is a set of cell surface molecules encoded by a large gene family which controls a major part of the immune system in all vertebrates. The major function of major histocompatibility complexes is to bind to peptide fragments derived from pathogens and display them on the cell surface for recognition by the appropriate T-cells. MHC molecules mediate interactions of leukocytes, also called white blood cells (WBCs), which are immune cells, with other leukocytes or with body cells. The MHC determines compatibility of donors for organ transplant, as well as one's susceptibility to an autoimmune disease via crossreacting immunization. In humans, the MHC is also called the human leukocyte antigen (HLA).In a cell, protein molecules of the host's own phenotype or of other biologic entities are continually synthesized and degraded. Each MHC molecule on the cell surface displays a molecular fraction of a protein, called epitope. The presented antigen can be either 'self' or 'nonself', thus preventing an organism`s immune system targeting its own cells. In its entirety, the MHC population is like a meter indicating the balance of proteins within the cell.The MHC gene family is divided into three subgroups: class I, class II, and class III. Class I MHC molecules have β2 subunits so can only be recognised by CD8 co-receptors. Class II MHC molecules have no β2 subunits so can be recognised by CD4 co-receptors. In this way MHC molecules chaperones which type of lymphocytes may bind to the given antigen with high affinity, since different lymphocytes express different TCR co-receptors. Diversity of antigen presentation, mediated by MHC classes I and II, is attained in at least three ways: (1) an organism's MHC repertoire is polygenic (via multiple, interacting genes); (2) MHC expression is codominant (from both sets of inherited alleles); (3) MHC gene variants are highly polymorphic (diversely varying from organism to organism within a species). Major histocompatibility complex and sexual selection has been observed in male mice making mate choices of females with different MHCs and thus demonstrating sexual selection.