Equine allogeneic bone marrow-derived mesenchymal stromal cells
... bone marrow-derived MSCs are used routinely in regenerative therapies for equine patients to treat musculoskeletal disorders including tendonitis, osteoarthritis, cartilage damage, and meniscal injuries [14-20]. In addition, the horse allows for noninvasive access to large quantities of samples such ...
... bone marrow-derived MSCs are used routinely in regenerative therapies for equine patients to treat musculoskeletal disorders including tendonitis, osteoarthritis, cartilage damage, and meniscal injuries [14-20]. In addition, the horse allows for noninvasive access to large quantities of samples such ...
Current and novel therapeutics in the treatment of systemic lupus erythematosus
... In mice rituximab targets B lymphocytes in vivo from the pre-B stage in the bone marrow when CD20 is first expressed to the mature naive and memory B-cell stages. In human subjects it clearly depletes B cells from peripheral blood, but the degree of tissue depletion is not precisely known. CD20 is n ...
... In mice rituximab targets B lymphocytes in vivo from the pre-B stage in the bone marrow when CD20 is first expressed to the mature naive and memory B-cell stages. In human subjects it clearly depletes B cells from peripheral blood, but the degree of tissue depletion is not precisely known. CD20 is n ...
production and quality control of monoclonal antibodies
... to establish that blood borne viruses such as HIV, hepatitis B and hepatitis C were not in the process of incubation. If these conditions can not be completely fulfilled, this should be justified and it should be demonstrated that the cell bank system is devoid of any relevant viruses (e.g. HIV 1/2, ...
... to establish that blood borne viruses such as HIV, hepatitis B and hepatitis C were not in the process of incubation. If these conditions can not be completely fulfilled, this should be justified and it should be demonstrated that the cell bank system is devoid of any relevant viruses (e.g. HIV 1/2, ...
CCAC guidelines on: antibody production, 2002
... animal use in research, teaching and testing. In addition to the Guide to the Care and Use of Experimental Animals,Vol. 1, 2nd Edn., 1993 and Vol. 2, 1984, which lay down general principles for the care and use of animals, the CCAC also publishes guidelines on issues of current and emerging concerns ...
... animal use in research, teaching and testing. In addition to the Guide to the Care and Use of Experimental Animals,Vol. 1, 2nd Edn., 1993 and Vol. 2, 1984, which lay down general principles for the care and use of animals, the CCAC also publishes guidelines on issues of current and emerging concerns ...
Rejection after Lung Transplantation?
... without warning. In some cases, you might notice symptoms like shortness of breath, cough or a low-grade fever. This is why you should report any new or worsening symptoms to your transplant team right away. The best way to detect rejection is by monitoring of your lung function with regular breathi ...
... without warning. In some cases, you might notice symptoms like shortness of breath, cough or a low-grade fever. This is why you should report any new or worsening symptoms to your transplant team right away. The best way to detect rejection is by monitoring of your lung function with regular breathi ...
... institutions. By the presence of Yayasan Lupus Indonesia (YLI), public awareness about Lupus increased. Many people consider Lupus as a rare disease and have few patients, but in fact, there is quite a lot of patients with this disease and is increasing. Before the existence of YLI, mortality rate d ...
Hantavirus (Hantaan)
... ELISA as well as Hantavirus Hantaan Antibody IF Test, Hantavirus Puumala Antibody IF Test, Hantavirus Seoul Antibody IF Test, revealed a diagnostic efficiencya as follows: ...
... ELISA as well as Hantavirus Hantaan Antibody IF Test, Hantavirus Puumala Antibody IF Test, Hantavirus Seoul Antibody IF Test, revealed a diagnostic efficiencya as follows: ...
Supplement to Supplement to Rheumatology News
... further proliferation. When cytokines, such as TNF, are locally produced in excess levels, enzymatic cleavage from the cell membrane releases them to enter the circulation, where they can cause remote effects, including stimulatory effects on bone marrow cells. Hence, the effects of cytokine blockad ...
... further proliferation. When cytokines, such as TNF, are locally produced in excess levels, enzymatic cleavage from the cell membrane releases them to enter the circulation, where they can cause remote effects, including stimulatory effects on bone marrow cells. Hence, the effects of cytokine blockad ...
Cell-Free Synthesis Meets Antibody Production: A Review
... selected against a desired antigen in a process called “panning”. Beyond phage display, several other display techniques have been evolved in the past, such as bacterial surface display [21], yeast surface display [22], messenger RNA (mRNA) display [23,24] and ribosome display [25,26], all enabling ...
... selected against a desired antigen in a process called “panning”. Beyond phage display, several other display techniques have been evolved in the past, such as bacterial surface display [21], yeast surface display [22], messenger RNA (mRNA) display [23,24] and ribosome display [25,26], all enabling ...
Cell-Free Synthesis Meets Antibody Production: A Review
... selected against a desired antigen in a process called “panning”. Beyond phage display, several other display techniques have been evolved in the past, such as bacterial surface display [21], yeast surface display [22], messenger RNA (mRNA) display [23,24] and ribosome display [25,26], all enabling ...
... selected against a desired antigen in a process called “panning”. Beyond phage display, several other display techniques have been evolved in the past, such as bacterial surface display [21], yeast surface display [22], messenger RNA (mRNA) display [23,24] and ribosome display [25,26], all enabling ...
Immunogenicity - Biologics and Biosimilars
... ADA Assay: The assay should: Run in parallel with PK studies To have sufficient sensitivity and specificity for non GLP Be validated for GLP studies Neutralizing Abs are typically not done in non-human primates but is considered in a case by case basis and depends upon a variety of factors that ...
... ADA Assay: The assay should: Run in parallel with PK studies To have sufficient sensitivity and specificity for non GLP Be validated for GLP studies Neutralizing Abs are typically not done in non-human primates but is considered in a case by case basis and depends upon a variety of factors that ...
Licentiate thesis from the Department of Immunology,
... Malaria is the most prevalent infectious disease in the world today, in regard to morbidity and mortality, and it is mostly affecting sub Saharan Africa. High priority is put on the development of a vaccine against the malaria parasite Plasmodium falciparum, which due to its prevalence, virulence an ...
... Malaria is the most prevalent infectious disease in the world today, in regard to morbidity and mortality, and it is mostly affecting sub Saharan Africa. High priority is put on the development of a vaccine against the malaria parasite Plasmodium falciparum, which due to its prevalence, virulence an ...
Bacterial Kidney Disease in salmonid fish
... giant cell (arrow), HE X 400. Photo: Paul Mbuthia. ...
... giant cell (arrow), HE X 400. Photo: Paul Mbuthia. ...
Improved Clonal Selection Algorithm (ICLONALG)
... 6) Recognition: of foreigners: the (harmful) molecules that are not native to the body are recognized and eliminated by the immune system. ...
... 6) Recognition: of foreigners: the (harmful) molecules that are not native to the body are recognized and eliminated by the immune system. ...
Immunity and how vaccines work
... Time intervals between vaccine doses • 2 Live vaccines - Minimum one month interval required – Allows each immune response to develop – Allows the next response to be a true secondary response – i.e. faster and bigger and with higher affinity IgG – Diminishes immune interference ...
... Time intervals between vaccine doses • 2 Live vaccines - Minimum one month interval required – Allows each immune response to develop – Allows the next response to be a true secondary response – i.e. faster and bigger and with higher affinity IgG – Diminishes immune interference ...
PDF - Journal of Veterinary Science
... using OMP and DNT of B. bronchiseptica as antigens. Generally, antibody titers peaked at 4 and 6 weeks in sera and egg yolks, respectively, after the first injection (Fig. 1 and 2). The titers of the vaccinated groups were three times higher than those of the control group. Antibody profiles against ...
... using OMP and DNT of B. bronchiseptica as antigens. Generally, antibody titers peaked at 4 and 6 weeks in sera and egg yolks, respectively, after the first injection (Fig. 1 and 2). The titers of the vaccinated groups were three times higher than those of the control group. Antibody profiles against ...
Cutaneous Lupus Erythematosus
... “housekeeping” macrophages,” and their intercellular contents are not available for immune surveillance. Dysregulation of this step can lead to induction of SLE in two ways Increased apoptosis - If clearance of apoptotic material is not accomplished quickly, cells become necrotic and release previ ...
... “housekeeping” macrophages,” and their intercellular contents are not available for immune surveillance. Dysregulation of this step can lead to induction of SLE in two ways Increased apoptosis - If clearance of apoptotic material is not accomplished quickly, cells become necrotic and release previ ...
The phenotype of alveolar macrophages ... with immune cells in bronchoalveolar ...
... Distinct phenotypic subpopulations of AMs have recently been identified [15, 16]. Macrophages with properties of dendritic, phagocytic or suppressive cells may be separated on the basis of the eo-expression of membrane antigens RFD1, RFD7 and RFD9 [17, 18]. Other subpopulations of AMs can be disting ...
... Distinct phenotypic subpopulations of AMs have recently been identified [15, 16]. Macrophages with properties of dendritic, phagocytic or suppressive cells may be separated on the basis of the eo-expression of membrane antigens RFD1, RFD7 and RFD9 [17, 18]. Other subpopulations of AMs can be disting ...
Immunological aspects of liver disease
... 1969, and providing important critical and enthusiastic support. In 1970, to add basic science depth to the research, Adrian, with the help of an MRC Travelling Fellowship, went to work with Bob Good in Minneapolis, USA, as an immunology research fellow, and was exposed for a year and a half to this ...
... 1969, and providing important critical and enthusiastic support. In 1970, to add basic science depth to the research, Adrian, with the help of an MRC Travelling Fellowship, went to work with Bob Good in Minneapolis, USA, as an immunology research fellow, and was exposed for a year and a half to this ...
IOSR Journal of Electrical and Electronics Engineering (IOSR-JEEE)
... encounter the repeated attacks by the same antigen during the entire lifetime of the individual.The initial exposure to an antigen induces an adaptive immune response that is initially handled by a small number of B-cells that produce antibodies of varying affinities.If we can store some high affini ...
... encounter the repeated attacks by the same antigen during the entire lifetime of the individual.The initial exposure to an antigen induces an adaptive immune response that is initially handled by a small number of B-cells that produce antibodies of varying affinities.If we can store some high affini ...
Sequential follow up observations of a patient with rubella
... Peptide coated microplate wells were subsequently exposed to 1:50 dilutions of patient or reference (15 IU/ml) or positive and negative control serum samples and incubated for 30 minutes at room temperature. After washing the plates with PBST, bound specific antibodies were detected by the sequentia ...
... Peptide coated microplate wells were subsequently exposed to 1:50 dilutions of patient or reference (15 IU/ml) or positive and negative control serum samples and incubated for 30 minutes at room temperature. After washing the plates with PBST, bound specific antibodies were detected by the sequentia ...
Review Article Bridging Innate and Adaptive Antitumor Immunity
... Figure 1: A. ELISA plates were coated with blood type B antigen and reactivity of Gal purified human antibodies detected. Human IVIg was used as control. ...
... Figure 1: A. ELISA plates were coated with blood type B antigen and reactivity of Gal purified human antibodies detected. Human IVIg was used as control. ...
Drug-induced immune thrombocytopenia
... with gold salts for rheumatoid arthritis [40]. Other medications implicated as possible triggers for AITP include L-dopa, procaine amide, penicillamine, and sulfamethoxazole (Fig. 2) [1,2,39]. How selected medications induce an autoimmune response against platelets is unknown. The suggestion has bee ...
... with gold salts for rheumatoid arthritis [40]. Other medications implicated as possible triggers for AITP include L-dopa, procaine amide, penicillamine, and sulfamethoxazole (Fig. 2) [1,2,39]. How selected medications induce an autoimmune response against platelets is unknown. The suggestion has bee ...
PDF
... palatal tissues of B10.A(H-2Kk) embryonic mice. In addition the fluorescent staining appeared to be cell specific, being associated with mesenchymal but not with epithelial cells. It remains obscure as to why the H-2 staining finally localized in the mesenchyme immediately subjacent to the nasal epi ...
... palatal tissues of B10.A(H-2Kk) embryonic mice. In addition the fluorescent staining appeared to be cell specific, being associated with mesenchymal but not with epithelial cells. It remains obscure as to why the H-2 staining finally localized in the mesenchyme immediately subjacent to the nasal epi ...
Anti-nuclear antibody
Antinuclear antibodies (ANAs, also known as antinuclear factor or ANF) are autoantibodies that bind to contents of the cell nucleus. In normal individuals, the immune system produces antibodies to foreign proteins (antigens) but not to human proteins (autoantigens). In some individuals, antibodies to human antigens are produced.There are many subtypes of ANAs such as anti-Ro antibodies, anti-La antibodies, anti-Sm antibodies, anti-nRNP antibodies, anti-Scl-70 antibodies, anti-dsDNA antibodies, anti-histone antibodies, antibodies to nuclear pore complexes, anti-centromere antibodies and anti-sp100 antibodies. Each of these antibody subtypes binds to different proteins or protein complexes within the nucleus. They are found in many disorders including autoimmunity, cancer and infection, with different prevalences of antibodies depending on the condition. This allows the use of ANAs in the diagnosis of some autoimmune disorders, including systemic lupus erythematosus, Sjögren's syndrome, scleroderma, mixed connective tissue disease, polymyositis, dermatomyositis, autoimmune hepatitis and drug induced lupus.The ANA test detects the autoantibodies present in an individual's blood serum. The common tests used for detecting and quantifying ANAs are indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA). In immunofluorescence, the level of autoantibodies is reported as a titre. This is the highest dilution of the serum at which autoantibodies are still detectable. Positive autoantibody titres at a dilution equal to or greater than 1:160 are usually considered as clinically significant. Positive titres of less than 1:160 are present in up to 20% of the healthy population, especially the elderly. Although positive titres of 1:160 or higher are strongly associated with autoimmune disorders, they are also found in 5% of healthy individuals. Autoantibody screening is useful in the diagnosis of autoimmune disorders and monitoring levels helps to predict the progression of disease. A positive ANA test is seldom useful if other clinical or laboratory data supporting a diagnosis are not present.