Success Story - Henry M. Jackson Foundation
... antibodies could prevent RSV infection. During an initial clinical study, a baby with severe pneumonia made a remarkable recovery after receiving IVIG. Fischer and his University collaborator, Colonel (Ret.) Val Hemming (chair, USU Department of Pediatrics; later Dean, School of Medicine), began ...
... antibodies could prevent RSV infection. During an initial clinical study, a baby with severe pneumonia made a remarkable recovery after receiving IVIG. Fischer and his University collaborator, Colonel (Ret.) Val Hemming (chair, USU Department of Pediatrics; later Dean, School of Medicine), began ...
Dilated cardiomyopathy (DCM) and myocarditis: Classification
... younger adults, is characterized by dilatation and impaired contraction of the left or both ventricles; it may be idiopathic, familial/genetic (20-30%), viral, and/or immune. On endomyocardial biopsy there is chronic inflammation in 30-40% of cases. Mutations in genes encoding myocyte structural pro ...
... younger adults, is characterized by dilatation and impaired contraction of the left or both ventricles; it may be idiopathic, familial/genetic (20-30%), viral, and/or immune. On endomyocardial biopsy there is chronic inflammation in 30-40% of cases. Mutations in genes encoding myocyte structural pro ...
In Vivo CD40-gp39 Interactions Are Essential for Thymus
... of gp39 by CD4 + T cells is the result of antigen presentation (16). Once expressed, gp39 binds to CD40 and reciprocally triggers B cell activation. The ability of a mAb specific for gp39, MR1, to block the capacity of gp39-bearing Th to activate B cells in vitro has implicated gp39 as an important ...
... of gp39 by CD4 + T cells is the result of antigen presentation (16). Once expressed, gp39 binds to CD40 and reciprocally triggers B cell activation. The ability of a mAb specific for gp39, MR1, to block the capacity of gp39-bearing Th to activate B cells in vitro has implicated gp39 as an important ...
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... in a wide variety of tissues from chimaeric mice. The results indicate that H-2 antigens satisfy, at least in part, most of the criteria required of an ideal cellular marker. These are that the marker should be cell-autonomous, cell-localized, easily demonstrated in tissue sections, ubiquitous in em ...
... in a wide variety of tissues from chimaeric mice. The results indicate that H-2 antigens satisfy, at least in part, most of the criteria required of an ideal cellular marker. These are that the marker should be cell-autonomous, cell-localized, easily demonstrated in tissue sections, ubiquitous in em ...
Immunohaematology - The Carter Center
... the needs of Medical Laboratory professionals and Blood Bank personnel for a material that comprise the theories and laboratory techniques concerning blood transfusion service. The Lecture Note is also important for health professionals in other disciplines as a reference related to blood transfusio ...
... the needs of Medical Laboratory professionals and Blood Bank personnel for a material that comprise the theories and laboratory techniques concerning blood transfusion service. The Lecture Note is also important for health professionals in other disciplines as a reference related to blood transfusio ...
Paraneoplastic Syndromes
... ● It is often difficult to diagnose a patient with a paraneoplastic syndrome because the signs and symptoms associated with the syndrome can present before cancer is detected. ● Paraneoplastic syndromes can affect any organ system in the body. Patients are often referred to a specialist in the field ...
... ● It is often difficult to diagnose a patient with a paraneoplastic syndrome because the signs and symptoms associated with the syndrome can present before cancer is detected. ● Paraneoplastic syndromes can affect any organ system in the body. Patients are often referred to a specialist in the field ...
Characterization of a surface antigen of Type="Italic
... E. nieschulzi sporozoites, we conclude that mcab 3C3 recognizes a surface antigen on these organisms. This was also confirmed by immunoprecipitation data, since mcab 3C3 immunoprecipitates a radioactive protein comigrating with polypeptide found by iodinating sporozoites via the lactoperoxidase proc ...
... E. nieschulzi sporozoites, we conclude that mcab 3C3 recognizes a surface antigen on these organisms. This was also confirmed by immunoprecipitation data, since mcab 3C3 immunoprecipitates a radioactive protein comigrating with polypeptide found by iodinating sporozoites via the lactoperoxidase proc ...
A robust, high-throughput assay to determine the phagocytic activity
... Biotinylated antigen was incubated with 1 μm fluorescent neutravidin beads (Invitrogen, F8776) overnight at 4°C. Beads were subsequently spun down and washed twice in PBSBSA in order to remove excess unbound antigen, and then resuspended at a final dilution of 1:100 in PBS-BSA. Antigen-coated beads ...
... Biotinylated antigen was incubated with 1 μm fluorescent neutravidin beads (Invitrogen, F8776) overnight at 4°C. Beads were subsequently spun down and washed twice in PBSBSA in order to remove excess unbound antigen, and then resuspended at a final dilution of 1:100 in PBS-BSA. Antigen-coated beads ...
chapter 5 complement
... lytic and inflammatory properties of antibodies. The CLASSICAL pathway is initiated by antigen-antibody complexes (via complement components C1, C4, and C2), while the activation of the ALTERNATE pathway (via components B, D and P), and the MBLECTIN ("mannan-binding lectin") pathway may be initiated ...
... lytic and inflammatory properties of antibodies. The CLASSICAL pathway is initiated by antigen-antibody complexes (via complement components C1, C4, and C2), while the activation of the ALTERNATE pathway (via components B, D and P), and the MBLECTIN ("mannan-binding lectin") pathway may be initiated ...
An Update on Immunosuppressive Medications in Transplantation
... was characterized by high incidence of acute rejection and low graft survival rate. The introduction of cyclosporine A in the 1980s represented a major advance in immunosuppressive therapy and substantially improved the 1-year graft survival rate. The so call triple immunosuppressive regimen, consis ...
... was characterized by high incidence of acute rejection and low graft survival rate. The introduction of cyclosporine A in the 1980s represented a major advance in immunosuppressive therapy and substantially improved the 1-year graft survival rate. The so call triple immunosuppressive regimen, consis ...
Antibody-Directed Phototherapy (ADP)
... Figure 2. Schematic diagram illustrating non-targeted and targeted PDT. After extravasation from the vasculature system PS-drugs pass from the blood into the tumour microenvironment where depending on their physical properties and to what (if anything) they are attached, they can either diffuse into ...
... Figure 2. Schematic diagram illustrating non-targeted and targeted PDT. After extravasation from the vasculature system PS-drugs pass from the blood into the tumour microenvironment where depending on their physical properties and to what (if anything) they are attached, they can either diffuse into ...
Bacillus anthracis Protective Antigen Bound by Neutralizing Antibodies *
... this region has been described to be recognized by two neutralizing mAbs (7, 9). With the exception of a neutralizing mAb that bound to PA20 (13), no B-cell epitopes have been reported in domain 1. Therefore, identification of further dominant antigenic epitopes is pivotal for understanding the mini ...
... this region has been described to be recognized by two neutralizing mAbs (7, 9). With the exception of a neutralizing mAb that bound to PA20 (13), no B-cell epitopes have been reported in domain 1. Therefore, identification of further dominant antigenic epitopes is pivotal for understanding the mini ...
In immunology, an adjuvant is an agent that may stimulate the
... on the unique ability of an antibody to bind with high specificity to one or a very limited group of molecules. A molecule that binds to an antibody is called an antigen. Immunoassays can be carried out for either member of an antigen/antibody pair. For antigen analytes, an antibody that specificall ...
... on the unique ability of an antibody to bind with high specificity to one or a very limited group of molecules. A molecule that binds to an antibody is called an antigen. Immunoassays can be carried out for either member of an antigen/antibody pair. For antigen analytes, an antibody that specificall ...
Epitope Specificity of Anti-Citrullinated Protein Antibodies
... In general, the pathogenesis of RA involves a number of cellular responses. Autoimmune responses mediated by T and B cells are important in the initiation of the inflammatory cascade. Then T cells, B cells, macrophages and neutrophils migrate into synovial tissues, where they, among others, produce ...
... In general, the pathogenesis of RA involves a number of cellular responses. Autoimmune responses mediated by T and B cells are important in the initiation of the inflammatory cascade. Then T cells, B cells, macrophages and neutrophils migrate into synovial tissues, where they, among others, produce ...
Detection of antibodies to common antigens of pathogenic and
... Neisseria spp. Bands in the 55-Kda and 35-Kda regions, specific to N . meningitidis, and one at 43 Kda specificto N . lactamica, were also frequently seen. The patterns of reactivity of antibodies in sera from carriers and non-carriers to antigens of three species of Neisseria are shown in fig. 2A a ...
... Neisseria spp. Bands in the 55-Kda and 35-Kda regions, specific to N . meningitidis, and one at 43 Kda specificto N . lactamica, were also frequently seen. The patterns of reactivity of antibodies in sera from carriers and non-carriers to antigens of three species of Neisseria are shown in fig. 2A a ...
Thyroid autoimmunity and polyglandular endocrine syndromes
... recognizes peptides of target organs. HLA alleles determine specific tissue targeting. B lymphocytes stimulated by T lymphocytes produce autoantibodies. T lymphocytes also contribute to cellular damage.22 The Major Histocompatibility System has the highest implication, being dependent on polymorphis ...
... recognizes peptides of target organs. HLA alleles determine specific tissue targeting. B lymphocytes stimulated by T lymphocytes produce autoantibodies. T lymphocytes also contribute to cellular damage.22 The Major Histocompatibility System has the highest implication, being dependent on polymorphis ...
handout
... complex formation) are not mutually exclusive and in humans more than one mechanism may contribute to injury. For example, in the case of postinfectious glomerulonephritis, in addition to glomerular injury caused by circulating immune complexes, there is also a component of in-situ immune complex de ...
... complex formation) are not mutually exclusive and in humans more than one mechanism may contribute to injury. For example, in the case of postinfectious glomerulonephritis, in addition to glomerular injury caused by circulating immune complexes, there is also a component of in-situ immune complex de ...
Natural Medicine and Nutritional Therapy as an Alternative
... agent of choice for active renal and neurological involvement. Indications for azathioprine were wide in spectrum and more generalized. At the conclusion of this study, 54 percent of the sample terminated the use of the cytotoxic agents, citing toxic side effects, remission, and failure to achieve a ...
... agent of choice for active renal and neurological involvement. Indications for azathioprine were wide in spectrum and more generalized. At the conclusion of this study, 54 percent of the sample terminated the use of the cytotoxic agents, citing toxic side effects, remission, and failure to achieve a ...
Epstein-Barr Virus (Powerpoint presentation)
... In 1968, Epstein-Barr Virus was actually identified as the cause of Infectious Mononucleosis Since the initial discovery, EBV has been implicated in a wide variety of both benign and malignant diseases ...
... In 1968, Epstein-Barr Virus was actually identified as the cause of Infectious Mononucleosis Since the initial discovery, EBV has been implicated in a wide variety of both benign and malignant diseases ...
Effect of Antigen/Antibody Ratio on Macrophage
... in extreme antibody excess, on the contrary, did not yield T cell activation although taken up by APC efficiently. The effect of antibodies at extreme excess was observed with substimulatory dose of antigen (loss of potentiation) and with optimal dose of antigen (loss of stimulation). An excess of s ...
... in extreme antibody excess, on the contrary, did not yield T cell activation although taken up by APC efficiently. The effect of antibodies at extreme excess was observed with substimulatory dose of antigen (loss of potentiation) and with optimal dose of antigen (loss of stimulation). An excess of s ...
Review Cytomegalovirus infection in patients with systemic lupus
... SLE patients have been performed by the measurement of viral antibodies and antigens using the enzyme-linked immunosorbent assay (ELISA) and the PCR, respectively (7, 18-20). Some investigations have suggested the possibility that virus-related antibodies are produced in SLE as a consequence of poly ...
... SLE patients have been performed by the measurement of viral antibodies and antigens using the enzyme-linked immunosorbent assay (ELISA) and the PCR, respectively (7, 18-20). Some investigations have suggested the possibility that virus-related antibodies are produced in SLE as a consequence of poly ...
“Best o Best o f DDW” f DDW
... Certolizumab pegol was detected in the e fetal circuit of 2 placentas placentas, certolizumab pegol in these samples (0.15% and 0.009%) was lower than the amount of anti-D IgG (0.60% and 0.39%, respectively) ...
... Certolizumab pegol was detected in the e fetal circuit of 2 placentas placentas, certolizumab pegol in these samples (0.15% and 0.009%) was lower than the amount of anti-D IgG (0.60% and 0.39%, respectively) ...
Antibody phage-displayed libraries derived from chicken
... A process known as hybridoma technology has been widely used to generate mAbs since its introduction by Köhler and Milstein in 1975. This method is laborious, often inefficient and is usually used to generate murine antibodies. This means that when used therapeutically they are likely to be immunoge ...
... A process known as hybridoma technology has been widely used to generate mAbs since its introduction by Köhler and Milstein in 1975. This method is laborious, often inefficient and is usually used to generate murine antibodies. This means that when used therapeutically they are likely to be immunoge ...
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... self-antigens are selected against to maintain tolerance; however, CNS antigens have been thought to be excluded from immune monitoring (17). Although this thinking has been recently challenged with the identification of both glymphatic and lymphatic circulation in the CNS (18, 19), re-exposure of t ...
... self-antigens are selected against to maintain tolerance; however, CNS antigens have been thought to be excluded from immune monitoring (17). Although this thinking has been recently challenged with the identification of both glymphatic and lymphatic circulation in the CNS (18, 19), re-exposure of t ...
Anti-nuclear antibody
Antinuclear antibodies (ANAs, also known as antinuclear factor or ANF) are autoantibodies that bind to contents of the cell nucleus. In normal individuals, the immune system produces antibodies to foreign proteins (antigens) but not to human proteins (autoantigens). In some individuals, antibodies to human antigens are produced.There are many subtypes of ANAs such as anti-Ro antibodies, anti-La antibodies, anti-Sm antibodies, anti-nRNP antibodies, anti-Scl-70 antibodies, anti-dsDNA antibodies, anti-histone antibodies, antibodies to nuclear pore complexes, anti-centromere antibodies and anti-sp100 antibodies. Each of these antibody subtypes binds to different proteins or protein complexes within the nucleus. They are found in many disorders including autoimmunity, cancer and infection, with different prevalences of antibodies depending on the condition. This allows the use of ANAs in the diagnosis of some autoimmune disorders, including systemic lupus erythematosus, Sjögren's syndrome, scleroderma, mixed connective tissue disease, polymyositis, dermatomyositis, autoimmune hepatitis and drug induced lupus.The ANA test detects the autoantibodies present in an individual's blood serum. The common tests used for detecting and quantifying ANAs are indirect immunofluorescence and enzyme-linked immunosorbent assay (ELISA). In immunofluorescence, the level of autoantibodies is reported as a titre. This is the highest dilution of the serum at which autoantibodies are still detectable. Positive autoantibody titres at a dilution equal to or greater than 1:160 are usually considered as clinically significant. Positive titres of less than 1:160 are present in up to 20% of the healthy population, especially the elderly. Although positive titres of 1:160 or higher are strongly associated with autoimmune disorders, they are also found in 5% of healthy individuals. Autoantibody screening is useful in the diagnosis of autoimmune disorders and monitoring levels helps to predict the progression of disease. A positive ANA test is seldom useful if other clinical or laboratory data supporting a diagnosis are not present.