Fatty Acid-Mediated Quorum Sensing Systems Stenotrophomonas maltophilia DOCTORAL THESIS
... 4.1.1 Two variants of rpf cluster with main differences in rpfF and rpfC genes are distributed among S. maltophilia population. ......................................................... 48 4.1.2 RpfC-1 but not RpfC-2 contains a transmembrane sensor input domain highly related to the Xcc RpfH-RpfC co ...
... 4.1.1 Two variants of rpf cluster with main differences in rpfF and rpfC genes are distributed among S. maltophilia population. ......................................................... 48 4.1.2 RpfC-1 but not RpfC-2 contains a transmembrane sensor input domain highly related to the Xcc RpfH-RpfC co ...
Pseudomonas diminuta - International Journal of Systematic and
... The original description of the genus Pseudomonas Migula 1894 allowed the inclusion of an extremely wide variety of aerobic gram-negative bacteria (24). The use of rRNA-based techniques, such as DNA-rRNA hybridization (12-14, 26) and 16s rRNA oligonucleotide cataloging (42), revealed the phylogeneti ...
... The original description of the genus Pseudomonas Migula 1894 allowed the inclusion of an extremely wide variety of aerobic gram-negative bacteria (24). The use of rRNA-based techniques, such as DNA-rRNA hybridization (12-14, 26) and 16s rRNA oligonucleotide cataloging (42), revealed the phylogeneti ...
Deoxyribonucleic Acid Homology Studies of Lactobacillus casei
... Agricultural Food Research Council Institute of Food Research, Reading Laboratory, ShinJield, Reading RG2 9AT, England Deoxyribonucleic acid (DNA)-DNA hybridizations were performed on strains of Lactobacillus casei. Our results indicate that this species as presently constituted is genomically very ...
... Agricultural Food Research Council Institute of Food Research, Reading Laboratory, ShinJield, Reading RG2 9AT, England Deoxyribonucleic acid (DNA)-DNA hybridizations were performed on strains of Lactobacillus casei. Our results indicate that this species as presently constituted is genomically very ...
Multiple sequence alignment for phylogenetic purposes
... (and therefore perversely fascinating) because I have always believed that there should be as much biology in a sequence alignment as there is mathematics and computing. This review is about just that idea, and is dedicated to putting biology back into the sequence alignment procedures used as part ...
... (and therefore perversely fascinating) because I have always believed that there should be as much biology in a sequence alignment as there is mathematics and computing. This review is about just that idea, and is dedicated to putting biology back into the sequence alignment procedures used as part ...
"Vinegar" as Anti-bacterial Biofilm formed by Streptococcus
... attached to living tissues that can be considered as an etiologic factor, among others. The knowledge about biofilm existence supports a new concept to explain chronic infections. Tonsillectomy is often the required choice as a consequence of frequent tonsillitis during the previous years, subsequen ...
... attached to living tissues that can be considered as an etiologic factor, among others. The knowledge about biofilm existence supports a new concept to explain chronic infections. Tonsillectomy is often the required choice as a consequence of frequent tonsillitis during the previous years, subsequen ...
Patterns, motifs, PSI-BLAST
... Consensus sequences • The consensus sequence method is the simplest method to build a model from a multiple sequence alignment. ...
... Consensus sequences • The consensus sequence method is the simplest method to build a model from a multiple sequence alignment. ...
Journal of Applied Microbiology
... of the 16S and the 5¢ end of the 23S rRNA genes was obtained by the band stab amplification technique, in which resolved PCR products, based on the 1491f (5¢-GAA GTC GTA ACA AGG TA-3¢) and L1r [5¢-CA(A/G) GGC ATC CAC CGT-3¢] primers, were retrieved directly from an agarose gel with a pipet tip aft ...
... of the 16S and the 5¢ end of the 23S rRNA genes was obtained by the band stab amplification technique, in which resolved PCR products, based on the 1491f (5¢-GAA GTC GTA ACA AGG TA-3¢) and L1r [5¢-CA(A/G) GGC ATC CAC CGT-3¢] primers, were retrieved directly from an agarose gel with a pipet tip aft ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... PCR products were separated by 2% agarose gel electrophoresis and examined under UV illumination. The PCR products were then sequenced. 2.3. Phylogenetic Analysis and Determination of the EPIYA Pattern The analysis included cagA 3′ region nucleotide sequences of 44 isolates of which 36 were Lombok i ...
... PCR products were separated by 2% agarose gel electrophoresis and examined under UV illumination. The PCR products were then sequenced. 2.3. Phylogenetic Analysis and Determination of the EPIYA Pattern The analysis included cagA 3′ region nucleotide sequences of 44 isolates of which 36 were Lombok i ...
Characterization of an Immuno-dominant Variable Surface
... was fractionated by preparative 5-15% gradient SDS-PAGE . After electrophoretic transfer to nitrocellulose the 125-kD region was excised from the blot, ground to a powder, and suspended in PBS. 100 141 of the suspension were diluted 1:9 with PBS and injected three times intraperitoneally into mice a ...
... was fractionated by preparative 5-15% gradient SDS-PAGE . After electrophoretic transfer to nitrocellulose the 125-kD region was excised from the blot, ground to a powder, and suspended in PBS. 100 141 of the suspension were diluted 1:9 with PBS and injected three times intraperitoneally into mice a ...
C. jejuni
... the largest and most ancient families with representatives in all extant phyla from prokaryotes to humans. ABC transporters are transmembrane proteins that utilize the energy of ATP hydrolysis to carry out certain biological processes including translocation of various substrates across membranes an ...
... the largest and most ancient families with representatives in all extant phyla from prokaryotes to humans. ABC transporters are transmembrane proteins that utilize the energy of ATP hydrolysis to carry out certain biological processes including translocation of various substrates across membranes an ...
Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3
... Background: Campylobacter jejuni and C. coli share a multitude of risk factors associated with human gastrointestinal disease, yet their phylogeny differs significantly. C. jejuni is scattered into several lineages, with no apparent linkage, whereas C. coli clusters into three distinct phylogenetic ...
... Background: Campylobacter jejuni and C. coli share a multitude of risk factors associated with human gastrointestinal disease, yet their phylogeny differs significantly. C. jejuni is scattered into several lineages, with no apparent linkage, whereas C. coli clusters into three distinct phylogenetic ...
Analyzing Amino-Acid Sequences
... Biochemical evidence of evolution compares favorably with structural evidence of evolution. Even organisms that appear to have few physical similarities may have similar sequences of amino acids in their proteins and be closely related through evolution. Many researchers believe that the greater the ...
... Biochemical evidence of evolution compares favorably with structural evidence of evolution. Even organisms that appear to have few physical similarities may have similar sequences of amino acids in their proteins and be closely related through evolution. Many researchers believe that the greater the ...
CHARACTERIZATION OF B-LACTAMASES FROM TWO B
... P.aeruginosa isolates (Z 33 and Z 90), where the maximum P-lactamase activities were 5.8 and 2.9 u mol/min/ml for the two isolates, respectively. This result was in agreement with the result of Cruchural et al., 1986. The results of the purification steps (Table 2, Fig 2(a.b) and Fig. 3(a,b) indicat ...
... P.aeruginosa isolates (Z 33 and Z 90), where the maximum P-lactamase activities were 5.8 and 2.9 u mol/min/ml for the two isolates, respectively. This result was in agreement with the result of Cruchural et al., 1986. The results of the purification steps (Table 2, Fig 2(a.b) and Fig. 3(a,b) indicat ...
Patent constraints
... story…a single patent may cover thousands of genes (Incyte Pharmaceuticals has IP rights over >2000 genes) Jensen and Murray, Science 310:239-240 (14 Oct. 2005) ...
... story…a single patent may cover thousands of genes (Incyte Pharmaceuticals has IP rights over >2000 genes) Jensen and Murray, Science 310:239-240 (14 Oct. 2005) ...
New Host Plants of Erwinia amylovora in Bulgaria
... der Zwet and Keil, 1979; Van der Zwet, 1996; Bonn and Van der Zwet, 2000). The causative agent is Erwinia amylovora. The bacterium affects mainly apple and pear, and other rosaceous plants of economic importance. Fire blight originated in North America but it has spread in many parts of the world, i ...
... der Zwet and Keil, 1979; Van der Zwet, 1996; Bonn and Van der Zwet, 2000). The causative agent is Erwinia amylovora. The bacterium affects mainly apple and pear, and other rosaceous plants of economic importance. Fire blight originated in North America but it has spread in many parts of the world, i ...
PDF
... PSSM for another similarity search. It has been demonstrated that much more distant homologs can be detected by iterating these steps. Because of its usefulness and availability, many modifications have been proposed since PSI-BLAST was first published, including introduction of compositionbased sta ...
... PSSM for another similarity search. It has been demonstrated that much more distant homologs can be detected by iterating these steps. Because of its usefulness and availability, many modifications have been proposed since PSI-BLAST was first published, including introduction of compositionbased sta ...
Extended spectrum beta-lactamases - Micro-Rao
... coli isolate obtained from urine specimen of a woman named Temoniera in Greece in 1965. This enzyme was designated as TEM-1. Within a decade this enzyme had spread to several members of Enterobacteriaceae as well as to Haemophilus influenzae and Neisseria gonnorheae. In 1969, TEM-2, a closely relate ...
... coli isolate obtained from urine specimen of a woman named Temoniera in Greece in 1965. This enzyme was designated as TEM-1. Within a decade this enzyme had spread to several members of Enterobacteriaceae as well as to Haemophilus influenzae and Neisseria gonnorheae. In 1969, TEM-2, a closely relate ...
Prokaryotic features of a nucleus
... the interesting question of whether or not these nuclear genes are of prokaryotic origin. Nucleus-encoded enzymes of the photosynthetic Calvin cycle are excellent marker molecules with which to investigate this question because their primary structures may be directly compared to the primary structu ...
... the interesting question of whether or not these nuclear genes are of prokaryotic origin. Nucleus-encoded enzymes of the photosynthetic Calvin cycle are excellent marker molecules with which to investigate this question because their primary structures may be directly compared to the primary structu ...
Synonomy of Peptococcus glycinophilus
... Peptococcus glycinophilus (Cardon and Barker 1946) Douglas 1957, was identical to that of strain ATCC 33270 (= VPI 5464), the type strain of Peptostreptococcus micros (Prevot 1933) Smith 1957. Because these two strains were 84% homologous as determined by deoxyribonucleic acid-deoxyribonucleic acid ...
... Peptococcus glycinophilus (Cardon and Barker 1946) Douglas 1957, was identical to that of strain ATCC 33270 (= VPI 5464), the type strain of Peptostreptococcus micros (Prevot 1933) Smith 1957. Because these two strains were 84% homologous as determined by deoxyribonucleic acid-deoxyribonucleic acid ...
Are You suprised ?
... organisms that appear to have few physical similarities may have similar sequences of amino acids in their proteins and be closely related through evolution. Many researchers believe that the greater the similarity in the amino-acid sequences of two organisms, the more closely related they are in an ...
... organisms that appear to have few physical similarities may have similar sequences of amino acids in their proteins and be closely related through evolution. Many researchers believe that the greater the similarity in the amino-acid sequences of two organisms, the more closely related they are in an ...
BIF CH4 5th proofs.qxd
... No one has yet devised a scoring scheme that perfectly models the evolutionary process, which is so complex that it defies any practical method of modeling. The implication of this is that the best-scoring alignment will not necessarily be the correct one, and conversely, that the correct alignment ...
... No one has yet devised a scoring scheme that perfectly models the evolutionary process, which is so complex that it defies any practical method of modeling. The implication of this is that the best-scoring alignment will not necessarily be the correct one, and conversely, that the correct alignment ...
Are You suprised ?
... organisms that appear to have few physical similarities may have similar sequences of amino acids in their proteins and be closely related through evolution. Many researchers believe that the greater the similarity in the amino-acid sequences of two organisms, the more closely related they are in an ...
... organisms that appear to have few physical similarities may have similar sequences of amino acids in their proteins and be closely related through evolution. Many researchers believe that the greater the similarity in the amino-acid sequences of two organisms, the more closely related they are in an ...
AMP-forming acetyl-CoA synthetases in Archaea show
... (Grayson and Westkaemper 1988, Horswill and EscalanteSemerena 2002). These structures demonstrate the enzyme in two different conformations. The structure of the yeast enzyme is thought to represent the conformation of the enzyme in the first step of the reaction, which involves acetate and ATP but ...
... (Grayson and Westkaemper 1988, Horswill and EscalanteSemerena 2002). These structures demonstrate the enzyme in two different conformations. The structure of the yeast enzyme is thought to represent the conformation of the enzyme in the first step of the reaction, which involves acetate and ATP but ...
Identifying producers of antibacterial compounds by
... The fingerprint GPA amplicons were sequenced and used to construct an unrooted Bayesian phylogenetic tree for the concatenated translated sequences of the three core GPA biosynthetic genes (oxyB, oxyC and dpgC) (Fig. 3c). The relatively short amplicons make estimation of phylogenetic trees for indiv ...
... The fingerprint GPA amplicons were sequenced and used to construct an unrooted Bayesian phylogenetic tree for the concatenated translated sequences of the three core GPA biosynthetic genes (oxyB, oxyC and dpgC) (Fig. 3c). The relatively short amplicons make estimation of phylogenetic trees for indiv ...
Shelef, Katie: A Critical Analysis of Degenerate Primer Design Programs
... reaction (PCR) has revolutionized the biological sciences. PCR can be used as a diagnostic tool in pathogen detection, genotyping, screening genes for mutations and identification of single nucleotide polymorphisms (SNPs), determining molecularly-defined evolutionary relationships among organisms, a ...
... reaction (PCR) has revolutionized the biological sciences. PCR can be used as a diagnostic tool in pathogen detection, genotyping, screening genes for mutations and identification of single nucleotide polymorphisms (SNPs), determining molecularly-defined evolutionary relationships among organisms, a ...