File - western undergrad. by the students, for the students.
... 2. The DNA sequences required for transcriptional termination are called the terminator. 3. Transcription is catalyzed by the enzyme RNA polymerase. 4. Gene specific regulatory proteins (or transcription factors) are the key molecules in the differential transcription of genes. Bacterial transcripti ...
... 2. The DNA sequences required for transcriptional termination are called the terminator. 3. Transcription is catalyzed by the enzyme RNA polymerase. 4. Gene specific regulatory proteins (or transcription factors) are the key molecules in the differential transcription of genes. Bacterial transcripti ...
Systems Biology Conceptual Modeling by Means of Discrete
... process in particular can be modeled as a discrete dynamic system. Two areas can benefit from such a methodology that has been presented in this paper: to stimulate research and to assist teaching. For the teaching purposes this can assist to visualize the protein production processes model from sta ...
... process in particular can be modeled as a discrete dynamic system. Two areas can benefit from such a methodology that has been presented in this paper: to stimulate research and to assist teaching. For the teaching purposes this can assist to visualize the protein production processes model from sta ...
Molecular Biology – Final Laboratory Report
... Gene Expression Profile Gel electrophoresis was performed on EtBr-stained samples from RT-PCR with JAL1 primers in order to determine the stages of the Tetrahymena life cycle during which the JAL1 gene is actively transcribed. Transcription levels at various time points provide strong indication of ...
... Gene Expression Profile Gel electrophoresis was performed on EtBr-stained samples from RT-PCR with JAL1 primers in order to determine the stages of the Tetrahymena life cycle during which the JAL1 gene is actively transcribed. Transcription levels at various time points provide strong indication of ...
Lecture 14: Protein and Fat Synthesis
... complex. Then, there is a condensation of the latter so that a 4 – C unit is produced. This unit by next three reactions i.e. reduction, dehydration and reduction is converted into saturated 4 – C unit (i.e. butyryl – CoA). In acyl transfer reaction the fatty acid residue is transferred back to the ...
... complex. Then, there is a condensation of the latter so that a 4 – C unit is produced. This unit by next three reactions i.e. reduction, dehydration and reduction is converted into saturated 4 – C unit (i.e. butyryl – CoA). In acyl transfer reaction the fatty acid residue is transferred back to the ...
MI Unit 2 Cram Sheet
... and to understand their inner workings, their most important part – DNA. DNA can now be isolated from cells and “picked apart” to reveal disease. Genetic testing can be used to diagnose disease before a child is even born. We can test ourselves for diseases and learn the likelihood of passing them o ...
... and to understand their inner workings, their most important part – DNA. DNA can now be isolated from cells and “picked apart” to reveal disease. Genetic testing can be used to diagnose disease before a child is even born. We can test ourselves for diseases and learn the likelihood of passing them o ...
Unit 2 Study Guide
... and to understand their inner workings, their most important part – DNA. DNA can now be isolated from cells and “picked apart” to reveal disease. Genetic testing can be used to diagnose disease before a child is even born. We can test ourselves for diseases and learn the likelihood of passing them o ...
... and to understand their inner workings, their most important part – DNA. DNA can now be isolated from cells and “picked apart” to reveal disease. Genetic testing can be used to diagnose disease before a child is even born. We can test ourselves for diseases and learn the likelihood of passing them o ...
Biol120 Mock Final Examination (v2.0)
... 43. Why is sugar oxidized in many small steps rather than burned all at once? a) To release more heat energy b) To transfer energy to other molecules c) To make the reaction become exergonic d) To increase the change in free energy 44. A non-functional allele for the gene for LDL receptor production ...
... 43. Why is sugar oxidized in many small steps rather than burned all at once? a) To release more heat energy b) To transfer energy to other molecules c) To make the reaction become exergonic d) To increase the change in free energy 44. A non-functional allele for the gene for LDL receptor production ...
What do genes do? - The Open University
... A protein is a linear sequence of amino acids. There is a direct and specific relationship between the linear sequence of base pairs that makes up a gene, and the linear sequence of amino acids in a protein molecule. This relationship, presented in a very simplistic manner in Figure 1, is known as t ...
... A protein is a linear sequence of amino acids. There is a direct and specific relationship between the linear sequence of base pairs that makes up a gene, and the linear sequence of amino acids in a protein molecule. This relationship, presented in a very simplistic manner in Figure 1, is known as t ...
5 min Insect DNA/RNA Preservation and Extraction Kit
... 5 min Insect DNA/RNA Preservation and Extraction Kit Biofactories’ 5 min Insect DNA/RNA Preservation and Extraction Kit provides the fastest method for the storage/preservation and isolation/purification of total DNA/RNA from insect samples. The kit is specially designed for preservation and extract ...
... 5 min Insect DNA/RNA Preservation and Extraction Kit Biofactories’ 5 min Insect DNA/RNA Preservation and Extraction Kit provides the fastest method for the storage/preservation and isolation/purification of total DNA/RNA from insect samples. The kit is specially designed for preservation and extract ...
Decoding Genetics - Flinn Scientific
... RNA polymerase II “reads” the DNA strand and creates a strand of messenger RNA (mRNA), which then travels out through the nuclear membrane to a ribosome in the cytoplasm of the cell. The ribosome binds to the mRNA strand at the start codon. The start codon is a three base-pair nucleotide sequence—ad ...
... RNA polymerase II “reads” the DNA strand and creates a strand of messenger RNA (mRNA), which then travels out through the nuclear membrane to a ribosome in the cytoplasm of the cell. The ribosome binds to the mRNA strand at the start codon. The start codon is a three base-pair nucleotide sequence—ad ...
Transcription in Prokaryotes
... lac repressor and causes an allosteric change resulting in loss of DNA binding activity. Glucose lowers the levels of cAMP, which is an allosteric effector of CAP. CAP does not interact with its binding site and the polymerase is recruited with low affinity leading to low levels of transcription. ...
... lac repressor and causes an allosteric change resulting in loss of DNA binding activity. Glucose lowers the levels of cAMP, which is an allosteric effector of CAP. CAP does not interact with its binding site and the polymerase is recruited with low affinity leading to low levels of transcription. ...
Kanr T-DNA Supplemental Figure 1. Transgenic complementation of
... Supplemental Figure 3. Generation of BCCP1- and BCCP2-specific antibodies. (A) Alignment of BCCP1 and BCCP2 amino acid sequences generated by the BESTFIT algorithm of GCG software package (Genetics Computer Group, Madison, WI). Identical residues are black-shaded and conservative substitutions are ...
... Supplemental Figure 3. Generation of BCCP1- and BCCP2-specific antibodies. (A) Alignment of BCCP1 and BCCP2 amino acid sequences generated by the BESTFIT algorithm of GCG software package (Genetics Computer Group, Madison, WI). Identical residues are black-shaded and conservative substitutions are ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.