Title: adaptive TCR Stuff name, name, institutions
... 3) MRD: DNA extracted from T cells isolated form 28 TALL patients pre-treatment and 29 days after treatment. PCR and Sequencing: TCRB chains from all samples were sequenced using Adaptive TCRs TCRB immunoSEQ assay (Fig. 1). ...
... 3) MRD: DNA extracted from T cells isolated form 28 TALL patients pre-treatment and 29 days after treatment. PCR and Sequencing: TCRB chains from all samples were sequenced using Adaptive TCRs TCRB immunoSEQ assay (Fig. 1). ...
Highly efficient semi-quantitative genotyping of single
... The PCR represents the initial analysis step. In comparison to many common technologies, unmodified primers can be used for amplification, which keeps assay costs low. The next step is the analysis of the PCR-amplified sequences by ICEMS. The nucleic acids are chromatographed as single strands in a ...
... The PCR represents the initial analysis step. In comparison to many common technologies, unmodified primers can be used for amplification, which keeps assay costs low. The next step is the analysis of the PCR-amplified sequences by ICEMS. The nucleic acids are chromatographed as single strands in a ...
Rapid and High Quality DNA Isolation from Origanum onites for
... from fresh or frozen Origanum onites leaves. In a single day, one person can complete the DNA isolation from more than 30 different leaf samples, and isolated DNA can be stored at Ð20 ∞C for a long period. The method yields large amounts of DNA (18Ð21 μg/200 mg fresh weight leaves), enough to conduc ...
... from fresh or frozen Origanum onites leaves. In a single day, one person can complete the DNA isolation from more than 30 different leaf samples, and isolated DNA can be stored at Ð20 ∞C for a long period. The method yields large amounts of DNA (18Ð21 μg/200 mg fresh weight leaves), enough to conduc ...
Lectures by Erin Barley Kathleen Fitzpatrick From Gene to Protein
... of its ability to base-pair with itself – Some bases in RNA contain functional groups that may participate in catalysis – RNA may hydrogen-bond with other nucleic acid molecules ...
... of its ability to base-pair with itself – Some bases in RNA contain functional groups that may participate in catalysis – RNA may hydrogen-bond with other nucleic acid molecules ...
RIBONUCLEIC ACID (RNA) NOTES
... Think of DNA as a prisoner inside the nucleus of the cell. DNA can never leave the nucleus as it must stay there to ...
... Think of DNA as a prisoner inside the nucleus of the cell. DNA can never leave the nucleus as it must stay there to ...
Applied Microbiology and Biotechnology
... genes are present in two different haloarchaeal genera. The products of these genes belong to a protein family with members in both bacteria and archaea, and are distinct from other bacterial intra-diol and extra-diol dioxygenases. Additional genes, which are highly conserved in both strains, and di ...
... genes are present in two different haloarchaeal genera. The products of these genes belong to a protein family with members in both bacteria and archaea, and are distinct from other bacterial intra-diol and extra-diol dioxygenases. Additional genes, which are highly conserved in both strains, and di ...
Transcription and Translation
... the genes code for proteins the proteins determine what a cell will be like The DNA stores this information safely in the nucleus where it never leaves instructions are copied from the DNA into messages comprised of RNA these messages are sent out into the cell to direct the assembly of prot ...
... the genes code for proteins the proteins determine what a cell will be like The DNA stores this information safely in the nucleus where it never leaves instructions are copied from the DNA into messages comprised of RNA these messages are sent out into the cell to direct the assembly of prot ...
Protein Synthesis Project 1516
... Genes are the units that determine inherited characteristics, such as hair color and blood type. Genes are lengths of DNA molecules that determine the structure of polypeptides (the building blocks of prote ...
... Genes are the units that determine inherited characteristics, such as hair color and blood type. Genes are lengths of DNA molecules that determine the structure of polypeptides (the building blocks of prote ...
A Model for Recognition Scheme between Double Stranded DNA
... ds DNA according to the coordinates of Arnott et a1. (2) and for an antiparallel two-stranded ~ structure. Since ds DNA has two kinds of pseudo 2-fold axes perpendicular to the helix axis, one on the plane of each base pair, the other between two adjacent base pairs, we considered only the antiparal ...
... ds DNA according to the coordinates of Arnott et a1. (2) and for an antiparallel two-stranded ~ structure. Since ds DNA has two kinds of pseudo 2-fold axes perpendicular to the helix axis, one on the plane of each base pair, the other between two adjacent base pairs, we considered only the antiparal ...
Genetics RNA and Protein Synthesis
... Transfer RNA (tRNA)- RNA molecules that transfer each amino acid to the ribosome for protein assembly ...
... Transfer RNA (tRNA)- RNA molecules that transfer each amino acid to the ribosome for protein assembly ...
Nucleotide Sequence of an Iron Superoxide Dismutase
... We have reported the isolation of a cDNA (2) for which the corresponding mRNA, called SAM46, accumulates in cultured soybean cells during cytokinin or auxin starvation (see Table I). This mRNA, which decreases rapidly in abundance following treatment of cytokinin-starved cells with 5 mM zeatin or fo ...
... We have reported the isolation of a cDNA (2) for which the corresponding mRNA, called SAM46, accumulates in cultured soybean cells during cytokinin or auxin starvation (see Table I). This mRNA, which decreases rapidly in abundance following treatment of cytokinin-starved cells with 5 mM zeatin or fo ...
Leishmania major Friedlin chromosome 1 has an unusual
... of 10 different repeats of 117–522 bp, which are separated by up to 200 kb (see Fig. 1), but no large (.50 bp) inverted repeats were found; three sets of these repeats (III, VII, and X) represent tandem duplication of protein-coding genes (RPS7, LCFACAS, and EIF-4A, respectively). One (VIII) represe ...
... of 10 different repeats of 117–522 bp, which are separated by up to 200 kb (see Fig. 1), but no large (.50 bp) inverted repeats were found; three sets of these repeats (III, VII, and X) represent tandem duplication of protein-coding genes (RPS7, LCFACAS, and EIF-4A, respectively). One (VIII) represe ...
Analysis of DNA polymerase activity in vitro using non
... oligodT sequence with few or no intermediate peaks (Figure 1B). However, when the CA repeat oligonucleotide was used, DNA synthesis with Klenow showed a diverse behavior: the substrate was not filled completely and intermediate peaks were evident, suggesting decrease in its processivity and therefor ...
... oligodT sequence with few or no intermediate peaks (Figure 1B). However, when the CA repeat oligonucleotide was used, DNA synthesis with Klenow showed a diverse behavior: the substrate was not filled completely and intermediate peaks were evident, suggesting decrease in its processivity and therefor ...
Sensitive and Sequence-Specific DNA Assays
... SPR (SPR) has been demonstrated as a powerful technique for rapid, sensitive, and label-free genetic analysis [1-5]. When the sensor surface is coated with a single sensing (probe) DNA, SPR can be used for both affinity binding studies (i.e., kinetic measurements) and concentration detection of a ta ...
... SPR (SPR) has been demonstrated as a powerful technique for rapid, sensitive, and label-free genetic analysis [1-5]. When the sensor surface is coated with a single sensing (probe) DNA, SPR can be used for both affinity binding studies (i.e., kinetic measurements) and concentration detection of a ta ...
Effects of high magnetic fields on in vitro transcription
... The T7 RNA Polymerase is made up of multiple groups and subgroups. These parts each play distinct roles in the transcription process. A common model for the T7 RNA polymerase describes the polymerase as a hand (Cheetham 1999) where the thumb, fingers, and palm are the major groups of the polymerase ...
... The T7 RNA Polymerase is made up of multiple groups and subgroups. These parts each play distinct roles in the transcription process. A common model for the T7 RNA polymerase describes the polymerase as a hand (Cheetham 1999) where the thumb, fingers, and palm are the major groups of the polymerase ...
Real-time polymerase chain reaction
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (Quantitative real-time PCR), semi-quantitatively, i.e. above/below a certain amount of DNA molecules (Semi quantitative real-time PCR) or qualitatively (Qualitative real-time PCR).Two common methods for the detection of PCR products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter which permits detection only after hybridization of the probe with its complementary sequence.The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abbreviation qPCR be used for quantitative real-time PCR and that RT-qPCR be used for reverse transcription–qPCR [1]. The acronym ""RT-PCR"" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all authors adhere to this convention.