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Laboratory Manual
Laboratory Manual

... Microbes are microscopic organisms that comprises either a single cell (unicellular), cell clusters, or no cell at all (acellular). Bacteria are often viewed as the cause of diseases in humans and animals. Some bacteria are useful, for example certain bacteria aids in digestion. Bacteria make up the ...
ANTIBIOTIC RESISTANCE: ORIGINS, EVOLUTION, SELECTION
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... Antibiotics were initially developed for the treatment of infectious diseases in people. Their miraculous effects led to their being solicited and used for the treatment of animals and eventually plants. The same ones are being used in all three areas. Thus, an enormous worldwide selective pressure ...
Microbial Culturing Kit Biotechnology Explorer Catalog #166-5020EDU explorer.bio-rad.com
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... 5. Place the plates upside down inside the incubator for 16–24 hours at 37°C. ...
Guidance Document on the Use of Information on
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... biofertilisers, biopesticides, biomining, biomass conversion, or oil recovery). It is limited in scope to bacteria that may exhibit properties pathogenic to human beings. Not included in the scope are environmental releases of known (potential) pathogens, e.g. vaccine strains. The document explores ...
Problems caused by biofilms
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... cm2 in regenerative sections of pasteurisers after 12 hours of operation. This will cause contamination for example with Streptococcus thermophilus, which is reported to reach a contamination level of about 107 cells per mL in milk after biofilms have developed for 8 hours in a pasteuriser (de Jong ...
SI PPT: Microbioal Growth
SI PPT: Microbioal Growth

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... But there are more virulent and dangerous forms; E. Coli is capable of acquiring DNA for virulence and toxicity from other organisms like viruses. ...
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BIO 225 Lab Manual - Bluegrass Community and Technical College
BIO 225 Lab Manual - Bluegrass Community and Technical College

... techniques, the procedures followed by microbiologists and healthcare workers to prevent contamination of cultures from outside sources and to prevent introduction of potentially disease-causing microbes (pathogens) into the human body. The methods for handling previously sterilized materials, for t ...
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... when heated to 60" and above. [14C]Glycinewas released from the internal pool of Strep. faecalis at 50". Spermine, spermidine, cadaverine and Mg2+ partially protected protoplasts against thermal lysis. It is generally agreed that the ability of thermophiles to grow at high temperatures is due, at le ...
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PDF ( 27 ) - DergiPark

... obtained from a farm where tetracycline and tylosin were used extensively. Samples were collected from the manure and soil, before and after manure application. All of the 151 bacteria were identified based on BLAST results. Minimum inhibitory concentrations were determined by agar dilution and E-te ...
VI.P.1 VI.P.2 Session VI. Environmental microbiology
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Indezine Template
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... • most microorganisms are harmless • some microorganisms found in the body are beneficial to us • when a microorganism enters a part of the body other than where it is intended to be, it can be harmful • eg. E. coli from the colon (breaks down waste/makes vitamin K) can enter the urinary tract and c ...
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... of a circular shape and contain two large plasmids which encode the most important virulence factors. One is called the pXO1 plasmid while the second is called the pXO2 plasmid (Moayeri 187). Each plasmid has a certain encoding “job” but each helps in replication and protection. ...
The L-cycle: a Process of Regeneration in Bacteria
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... stained normal bacillary filaments and the faded filaments which give rise to more and more granules can be well distinguished in this illustration. When a t the stage depicted in PI. 4, figs. 23 and 24 the cultures are reincubated at 32’ the still untransformed swarmers become vigorously motile. Th ...
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Diagnostic Laboratory Tests

... Sabouraud dextrose agar – used for isolation, identification and maintenance of pathogenic and non-pathogenic fungi. Contains dextrose, a nitrogen source, agar and acid pH of 5.6, which inhibits many bacteria present in mycological specimens. Various inhibitory agens may be incorporated to restrict ...
difference between light and electron microscopes - E
difference between light and electron microscopes - E

...  Microbiology is a cohesive science because of its methodology and approach to problems, not because of the relatedness of the organisms it studies.  Bacteria and archaea are prokaryotes; they lack internal membrane-bound structures.  Algae, fungi and protozoa are eukaryotes; their organelles are ...
A life in slime – biofilms rule the world
A life in slime – biofilms rule the world

... proven so valuable in the laboratory are virtually absent in nature: >99.9% of bacteria exist in heterogeneous communities called biofilms. ...
Strep Throat - Sarpy/Cass Health Department
Strep Throat - Sarpy/Cass Health Department

... Strep throat is a type of bacterial illness that causes an infection in the throat and tonsils. The bacterium can cause a wide range of infections. Many people can carry Group A Streptococcus (GAS) the throat or on their skin and have no symptoms of illness. Most GAS infections are relatively mild i ...
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Bacteria



Bacteria (/bækˈtɪəriə/; singular: bacterium) constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria have a number of shapes, ranging from spheres to rods and spirals. Bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep portions of Earth's crust. Bacteria also live in symbiotic and parasitic relationships with plants and animals. They are also known to have flourished in manned spacecraft.There are typically 40 million bacterial cells in a gram of soil and a million bacterial cells in a millilitre of fresh water. There are approximately 5×1030 bacteria on Earth, forming a biomass which exceeds that of all plants and animals. Bacteria are vital in recycling nutrients, with many of the stages in nutrient cycles dependent on these organisms, such as the fixation of nitrogen from the atmosphere and putrefaction. In the biological communities surrounding hydrothermal vents and cold seeps, bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. On 17 March 2013, researchers reported data that suggested bacterial life forms thrive in the Mariana Trench, which with a depth of up to 11 kilometres is the deepest part of the Earth's oceans. Other researchers reported related studies that microbes thrive inside rocks up to 580 metres below the sea floor under 2.6 kilometres of ocean off the coast of the northwestern United States. According to one of the researchers, ""You can find microbes everywhere — they're extremely adaptable to conditions, and survive wherever they are.""Most bacteria have not been characterized, and only about half of the phyla of bacteria have species that can be grown in the laboratory. The study of bacteria is known as bacteriology, a branch of microbiology.There are approximately ten times as many bacterial cells in the human flora as there are human cells in the body, with the largest number of the human flora being in the gut flora, and a large number on the skin. The vast majority of the bacteria in the body are rendered harmless by the protective effects of the immune system, and some are beneficial. However, several species of bacteria are pathogenic and cause infectious diseases, including cholera, syphilis, anthrax, leprosy, and bubonic plague. The most common fatal bacterial diseases are respiratory infections, with tuberculosis alone killing about 2 million people per year, mostly in sub-Saharan Africa. In developed countries, antibiotics are used to treat bacterial infections and are also used in farming, making antibiotic resistance a growing problem. In industry, bacteria are important in sewage treatment and the breakdown of oil spills, the production of cheese and yogurt through fermentation, and the recovery of gold, palladium, copper and other metals in the mining sector, as well as in biotechnology, and the manufacture of antibiotics and other chemicals.Once regarded as plants constituting the class Schizomycetes, bacteria are now classified as prokaryotes. Unlike cells of animals and other eukaryotes, bacterial cells do not contain a nucleus and rarely harbour membrane-bound organelles. Although the term bacteria traditionally included all prokaryotes, the scientific classification changed after the discovery in the 1990s that prokaryotes consist of two very different groups of organisms that evolved from an ancient common ancestor. These evolutionary domains are called Bacteria and Archaea.
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