High-Level Expression in Escherichia coli of Alkaline Phosphatase
... amino acid residues. The 5' (N-terminal) primer, AN2, 5'NNNNGAATTCAATGCTGCAGAACCAGCCTTCCTTG3', added a unique EcoRI site (underlined) and translation initiation codon, ATG, followed by the sequence coding the LeuGlnAsnGlnProSerLeu of the proposed N-terminal amino acid sequence of mature Tca APase [9 ...
... amino acid residues. The 5' (N-terminal) primer, AN2, 5'NNNNGAATTCAATGCTGCAGAACCAGCCTTCCTTG3', added a unique EcoRI site (underlined) and translation initiation codon, ATG, followed by the sequence coding the LeuGlnAsnGlnProSerLeu of the proposed N-terminal amino acid sequence of mature Tca APase [9 ...
Geminivirus Replication Origins Have a Modular
... their A components encode trans-acting factors required for this process. We showed that virus-specific interactions between the cis- and trans-acting functions are required for TGMV and BGMV replication in tobacco protoplasts. We also demonstrated that, similar to the essential TGMV AL1 replication ...
... their A components encode trans-acting factors required for this process. We showed that virus-specific interactions between the cis- and trans-acting functions are required for TGMV and BGMV replication in tobacco protoplasts. We also demonstrated that, similar to the essential TGMV AL1 replication ...
DNA phosphorothioation inStreptomyces lividans: mutational
... sylated [1-3]. Altered or unusual bases in DNA molecules often have significant physiological implications, such as DNA replication control, gene regulation, or protection of the respective organisms from invasion by foreign DNA [4]. Page 1 of 8 (page number not for citation purposes) ...
... sylated [1-3]. Altered or unusual bases in DNA molecules often have significant physiological implications, such as DNA replication control, gene regulation, or protection of the respective organisms from invasion by foreign DNA [4]. Page 1 of 8 (page number not for citation purposes) ...
New peptide and gene coding for same
... antihypertensive action, the DNA fragments are useful for the industrial production of 7-hANP and a-hANP and other diuretic peptides having an amino acid sequence present in the 7-hANP. For the construction of a recombinant DNA containing a base sequence coding for 7-hANP and useful for expression o ...
... antihypertensive action, the DNA fragments are useful for the industrial production of 7-hANP and a-hANP and other diuretic peptides having an amino acid sequence present in the 7-hANP. For the construction of a recombinant DNA containing a base sequence coding for 7-hANP and useful for expression o ...
FLUORESCENT PROTEIN IN Escherichia coli
... 1.1 Antimicrobial peptides . . . . . . . . . . . . . . . . . . . 1.1.1 Mechanism of action . . . . . . . . . . . . . . . . 1.1.2 Potential as drugs . . . . . . . . . . . . . . . . . . ...
... 1.1 Antimicrobial peptides . . . . . . . . . . . . . . . . . . . 1.1.1 Mechanism of action . . . . . . . . . . . . . . . . 1.1.2 Potential as drugs . . . . . . . . . . . . . . . . . . ...
Gene transfer in bacteria - McGraw Hill Higher Education
... Most are crucial to maintenance of earth's environment • Release oxygen to atmosphere • Recycle carbon, nitrogen, and other elements • Digest human and other animal waste • Neutralize pesticides and other pollutants • Produce vitamins and other materials essential to humans and other organisms Copyr ...
... Most are crucial to maintenance of earth's environment • Release oxygen to atmosphere • Recycle carbon, nitrogen, and other elements • Digest human and other animal waste • Neutralize pesticides and other pollutants • Produce vitamins and other materials essential to humans and other organisms Copyr ...
Protein export elements from Lactococcus lactis
... Two plasmids (Fig. I) were constructed that carried either the Bacillus licheniformis a-amylase gene (pGAI4), or the E. coli TEM-13-lactamase gene (pGBI4). The reporter genes were 5'-terminally truncated and lacked their transcription/translation initiation signals and the signal sequence, which wer ...
... Two plasmids (Fig. I) were constructed that carried either the Bacillus licheniformis a-amylase gene (pGAI4), or the E. coli TEM-13-lactamase gene (pGBI4). The reporter genes were 5'-terminally truncated and lacked their transcription/translation initiation signals and the signal sequence, which wer ...
Expression and purification of four different rhizobial acyl carrier
... synthesis of polyunsaturated fatty acids (Ritsema et al., 1998). Furthermore, these authors have shown that the NodF-specific functions are encoded in the C-terminal half of the protein. The presence in rhizobia of four different ACPs offers an unusual opportunity for investigation of the structure– ...
... synthesis of polyunsaturated fatty acids (Ritsema et al., 1998). Furthermore, these authors have shown that the NodF-specific functions are encoded in the C-terminal half of the protein. The presence in rhizobia of four different ACPs offers an unusual opportunity for investigation of the structure– ...
File
... The data recorded on the attached sheet shows that thirty-five colonies of transformed E. Coli bacteria grew on the Luria agar plate without ampicillin, a lawn (colonies of bacteria too dense to count) of non-transformed bacteria grew on the control plate without ampicillin, fifty-five colonies of t ...
... The data recorded on the attached sheet shows that thirty-five colonies of transformed E. Coli bacteria grew on the Luria agar plate without ampicillin, a lawn (colonies of bacteria too dense to count) of non-transformed bacteria grew on the control plate without ampicillin, fifty-five colonies of t ...
MARKER GENE TECHNOLOGIES, Inc
... We do not recommend reusing RedView precast gels as signal decreases with ...
... We do not recommend reusing RedView precast gels as signal decreases with ...
A series of promoters for constitutive expression of heterologous
... Inducible/repressible promoters are useful for the maintenance of toxic genes or timely expression. For ectopic expression of cloned genes in the fission yeast Schizosaccharomyces pombe, the thiamine-regulatable nmt1 promoter has been widely used, since the transcriptional activity of this promoter ...
... Inducible/repressible promoters are useful for the maintenance of toxic genes or timely expression. For ectopic expression of cloned genes in the fission yeast Schizosaccharomyces pombe, the thiamine-regulatable nmt1 promoter has been widely used, since the transcriptional activity of this promoter ...
Suppressors of Yeast Actin Mutations.
... that have, in addition, a conditional-lethal phenotype as a way to avoid these difficulties. In their method, revertants of a conditionally lethal mutant are screened for a growth defect under a new condition. They reverted cold-sensitive mutants defective in assembly of phage P22, and screened for ...
... that have, in addition, a conditional-lethal phenotype as a way to avoid these difficulties. In their method, revertants of a conditionally lethal mutant are screened for a growth defect under a new condition. They reverted cold-sensitive mutants defective in assembly of phage P22, and screened for ...
The first true obligately syntrophic propionate
... were inoculated with 0?2 g freeze-dried granular sludge. After addition of 20 mM sodium propionate and 10 % (v/v) of a H2/CO2-grown culture of Methanospirillum hungatei JF-1T, the enrichment culture was incubated at 37 uC. After 3 months, the propionate was depleted and CH4 was produced. Acetate was ...
... were inoculated with 0?2 g freeze-dried granular sludge. After addition of 20 mM sodium propionate and 10 % (v/v) of a H2/CO2-grown culture of Methanospirillum hungatei JF-1T, the enrichment culture was incubated at 37 uC. After 3 months, the propionate was depleted and CH4 was produced. Acetate was ...
Surface polysaccharide mutants of Rhizobium sp. (Acacia) strain
... GRH2-14 and GRH2-50. A Southern blot of EcoRIdigested total DNA of GRH2-14 and GRH2-50 mutant strains, probed with Tn5, showed a single insertion located in 2.4 and 3-2 kb EcoRI fragments, respectively (data not shown). In addition, Southern blotting an electrophoretic gel of Eckhart lysates from th ...
... GRH2-14 and GRH2-50. A Southern blot of EcoRIdigested total DNA of GRH2-14 and GRH2-50 mutant strains, probed with Tn5, showed a single insertion located in 2.4 and 3-2 kb EcoRI fragments, respectively (data not shown). In addition, Southern blotting an electrophoretic gel of Eckhart lysates from th ...
Microbiology
... GRH2-14 and GRH2-50. A Southern blot of EcoRIdigested total DNA of GRH2-14 and GRH2-50 mutant strains, probed with Tn5, showed a single insertion located in 2.4 and 3-2 kb EcoRI fragments, respectively (data not shown). In addition, Southern blotting an electrophoretic gel of Eckhart lysates from th ...
... GRH2-14 and GRH2-50. A Southern blot of EcoRIdigested total DNA of GRH2-14 and GRH2-50 mutant strains, probed with Tn5, showed a single insertion located in 2.4 and 3-2 kb EcoRI fragments, respectively (data not shown). In addition, Southern blotting an electrophoretic gel of Eckhart lysates from th ...
Exam 2
... b. Would this trick work if the only selectable marker on pPH1JI was TetR? [Explain why or why not]. ...
... b. Would this trick work if the only selectable marker on pPH1JI was TetR? [Explain why or why not]. ...
Update on Carbapenem Resistant Enterobacteriaceae
... • Enterobacteriaceae are gram-negative bacteria that can cause invasive disease but generally have been susceptible to a variety of antibiotics. • Carbapenem-Resistant Enterobacteriaceae (CRE) are Enterobacteriaceae that have become highly resistant to most or all antibiotics through several mechani ...
... • Enterobacteriaceae are gram-negative bacteria that can cause invasive disease but generally have been susceptible to a variety of antibiotics. • Carbapenem-Resistant Enterobacteriaceae (CRE) are Enterobacteriaceae that have become highly resistant to most or all antibiotics through several mechani ...
An Investigation of Acetobacter aceti N5
... the role of two conserved histidines. A pH-rate comparison of AaPurE and the active mutant AaPurE-H59D was used to identify His59 as the key active site acid/base residue. The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was fo ...
... the role of two conserved histidines. A pH-rate comparison of AaPurE and the active mutant AaPurE-H59D was used to identify His59 as the key active site acid/base residue. The thermostability of Escherichia coli PurE (EcPurE) over a range of pH was also assessed and compared to AaPurE. AaPurE was fo ...
Protein A gene expression is regulated by DNA supercoiling which
... (Dorman, 1991; Hsieh et al., 1991). In Escherichia coli, it has been estimated that roughly 50 % of the supercoiling is constrained by proteins bound to DNA. The remaining supercoils are maintained actively at the cost of ATP hydrolysis, via topoisomerase activities (Hatfield & Benham, 2002). Thus, ...
... (Dorman, 1991; Hsieh et al., 1991). In Escherichia coli, it has been estimated that roughly 50 % of the supercoiling is constrained by proteins bound to DNA. The remaining supercoils are maintained actively at the cost of ATP hydrolysis, via topoisomerase activities (Hatfield & Benham, 2002). Thus, ...
Ingestion of bacterially expressed dsRNAs can produce specific and
... L4431 (pPD129.17) contains two copies of the 0.8 kb GFP coding region in tail-to-tail inverted orientation, separated by 0.8 kb of unc-22 gene. A T7 promoter and T7 transcriptional terminator ¯ank the gfp inverted repeat. pLT61 contains 0.8 kb of unc-22 inserted into the L4440 vector. pLT76 contains ...
... L4431 (pPD129.17) contains two copies of the 0.8 kb GFP coding region in tail-to-tail inverted orientation, separated by 0.8 kb of unc-22 gene. A T7 promoter and T7 transcriptional terminator ¯ank the gfp inverted repeat. pLT61 contains 0.8 kb of unc-22 inserted into the L4440 vector. pLT76 contains ...
A systemic gene silencing method suitable for high throughput
... nurtures the young embryo. We have used the homosporous fern Ceratopteris richardii as a model system for studying gametophyte development because Ceratopteris gametophytes are autotrophic, small (~1 mm) and develop rapidly [1]. They can also be manipulated to develop as males or hermaphrodites by t ...
... nurtures the young embryo. We have used the homosporous fern Ceratopteris richardii as a model system for studying gametophyte development because Ceratopteris gametophytes are autotrophic, small (~1 mm) and develop rapidly [1]. They can also be manipulated to develop as males or hermaphrodites by t ...
UNIT – I: NUCLEIC ACID AND PROTEIN SYNTHESIS AND
... used in the development and functioning of all known living organisms. The main role of DNA molecules is the long-term storage of information and DNA is often compared to a set of blueprints, since it contains the instructions needed to construct other components of cells, such as proteins and RNA m ...
... used in the development and functioning of all known living organisms. The main role of DNA molecules is the long-term storage of information and DNA is often compared to a set of blueprints, since it contains the instructions needed to construct other components of cells, such as proteins and RNA m ...
Applied and Environmental Microbiologyy
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
Antibiotic resistance mechanisms in Enterobacteriaceae
... Carbapenemases are beta-lactamases with a wide hydrolytic spectrum. These enzymes inactivate almost all hydrolyzable beta-lactams including the carbapenems as a unique, additional substrate [27]. Carbapenemases are among beta-lactamases from Ambler classe A, B and D [7]. In class A, the dominant car ...
... Carbapenemases are beta-lactamases with a wide hydrolytic spectrum. These enzymes inactivate almost all hydrolyzable beta-lactams including the carbapenems as a unique, additional substrate [27]. Carbapenemases are among beta-lactamases from Ambler classe A, B and D [7]. In class A, the dominant car ...
Lactococcus lactis LM0230 contains a single
... step of many amino acids, may have important physiological functions in Lactococcus lactis during growth in milk. In this study, the aspartate ATase gene (aspC) from L. lactis LM0230 was cloned by complementation into Escherichia coli DL39. One chromosomal fragment putatively encoding aspC was parti ...
... step of many amino acids, may have important physiological functions in Lactococcus lactis during growth in milk. In this study, the aspartate ATase gene (aspC) from L. lactis LM0230 was cloned by complementation into Escherichia coli DL39. One chromosomal fragment putatively encoding aspC was parti ...
Plasmid
A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.