FEMS Microbiology Letters
... effects of Cr depend on its oxidation state; Cr(VI) is highly soluble and is considered the most toxic form of chromium (Cervantes et al., 2001). Numerous bacterial species have developed resistance to chromate that can be associated with chromosomal or plasmid-encoded genes (Ramı́rez-Dı́az et al., ...
... effects of Cr depend on its oxidation state; Cr(VI) is highly soluble and is considered the most toxic form of chromium (Cervantes et al., 2001). Numerous bacterial species have developed resistance to chromate that can be associated with chromosomal or plasmid-encoded genes (Ramı́rez-Dı́az et al., ...
$doc.title
... Klinghoffer, et al. “Reduced seed region-based off-target activity with lentivirus-mediated RNAi (2010) RNA 16:879-884. ...
... Klinghoffer, et al. “Reduced seed region-based off-target activity with lentivirus-mediated RNAi (2010) RNA 16:879-884. ...
Bacterial conjugation
... including penicillin, tetracycline, sulfanilamide, streptomycin, and chloramphenicol. This multiple-drugresistance phenotype was inherited as a single genetic package, and it could be transmitted in an infectious manner—not only to other sensitive Shigella strains, but also to other related species ...
... including penicillin, tetracycline, sulfanilamide, streptomycin, and chloramphenicol. This multiple-drugresistance phenotype was inherited as a single genetic package, and it could be transmitted in an infectious manner—not only to other sensitive Shigella strains, but also to other related species ...
Production of Recombinant Molecules
... understanding the fundamental concepts of the topics. The book is self-explanatory and adopts the “Teach Yourself” style. It is based on question-answer pattern. The language of book is quite easy and understandable based on scientific approach. Any further improvement in the contents of the book by ...
... understanding the fundamental concepts of the topics. The book is self-explanatory and adopts the “Teach Yourself” style. It is based on question-answer pattern. The language of book is quite easy and understandable based on scientific approach. Any further improvement in the contents of the book by ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... 2.3.1. DNA digestion with restriction enzymes Restriction endonucleases are enzymes isolated and purified from bacteria or fungi which bind specifically to and cleave double-stranded DNA at specific sites within or adjacent to a particular sequence known as the recognition sequence. The most used re ...
... 2.3.1. DNA digestion with restriction enzymes Restriction endonucleases are enzymes isolated and purified from bacteria or fungi which bind specifically to and cleave double-stranded DNA at specific sites within or adjacent to a particular sequence known as the recognition sequence. The most used re ...
CHAPTER 13 DNA manipulation
... isolated from that species, II for the second, and so on. In some cases an additional letter is added, such as in Hin dII, and the letter ‘d’ denotes a particular strain or type (serotype) of the bacterium. ...
... isolated from that species, II for the second, and so on. In some cases an additional letter is added, such as in Hin dII, and the letter ‘d’ denotes a particular strain or type (serotype) of the bacterium. ...
Biology, 8th Edition
... Recombinant DNA forms when DNA is spliced into a vector In recombinant DNA technology, geneticists cut both foreign DNA and plasmid DNA with the same restriction enzyme. The two types of DNA are then mixed under conditions that facilitate hydrogen bonding between the complementary bases of the stick ...
... Recombinant DNA forms when DNA is spliced into a vector In recombinant DNA technology, geneticists cut both foreign DNA and plasmid DNA with the same restriction enzyme. The two types of DNA are then mixed under conditions that facilitate hydrogen bonding between the complementary bases of the stick ...
DNA purification and isolation of genomic DNA from bacterial
... Max Yield System). All of these systems purify genomic DNA that is amenable for use in many downstream applications. Although techniques like Southern blotting, which require microgram amounts of DNA, are still performed in molecular biology laboratories, most assessment of chromosomal DNA is done b ...
... Max Yield System). All of these systems purify genomic DNA that is amenable for use in many downstream applications. Although techniques like Southern blotting, which require microgram amounts of DNA, are still performed in molecular biology laboratories, most assessment of chromosomal DNA is done b ...
100 Antibiotic.p65
... 2. a) (i) gram positive bacteria retain crystal violet in their cells during a gram stain but gram negative bacteria do not; for example, Bacillus sps/Clostridium sps are gram positive; Pseudomonas sps/Escherichia sps are gram negative; ref to higher content of peptidoglycan in gram positive bacteri ...
... 2. a) (i) gram positive bacteria retain crystal violet in their cells during a gram stain but gram negative bacteria do not; for example, Bacillus sps/Clostridium sps are gram positive; Pseudomonas sps/Escherichia sps are gram negative; ref to higher content of peptidoglycan in gram positive bacteri ...
Isolating and Analyzing Genes
... The first genes isolated were bacterial genes that could be picked up by bacteriophage. By isolating these hybrid bacteriophage, the DNA for the bacterial gene could be recovered in a highly enriched form. This is the basic principal behind recombinant DNA technology. Some bacteriophage will integra ...
... The first genes isolated were bacterial genes that could be picked up by bacteriophage. By isolating these hybrid bacteriophage, the DNA for the bacterial gene could be recovered in a highly enriched form. This is the basic principal behind recombinant DNA technology. Some bacteriophage will integra ...
Plant and Soil.
... relatedness, although the exact level below which organisms are considered to belong to different species varies, Total DNA-homology as revealed by DNADNA hybridization seems not to be in close agreement with 16S ribosomal sequence phylogeny in some cases. This is evident in Table 2 which shows DNA- ...
... relatedness, although the exact level below which organisms are considered to belong to different species varies, Total DNA-homology as revealed by DNADNA hybridization seems not to be in close agreement with 16S ribosomal sequence phylogeny in some cases. This is evident in Table 2 which shows DNA- ...
Inactivation of Antibiotics and the Dissemination of
... because the slow-growing mycobacteria possess only single copies of the rRNA genes, streptomycin resistance can arise by mutational alteration of either 16S rRNA or ribosomal protein S12. Both types of mutation have been identified in M. tubercubists (6). It is of note that Bacteria resistant to str ...
... because the slow-growing mycobacteria possess only single copies of the rRNA genes, streptomycin resistance can arise by mutational alteration of either 16S rRNA or ribosomal protein S12. Both types of mutation have been identified in M. tubercubists (6). It is of note that Bacteria resistant to str ...
View as PDF
... Chromatin structure plays an important role in the regulation of gene expression in the eukaryotic cell. Cytological studies have long suggested that different domains of the genome are packaged into two structurally different types of chromatin, heterochromatin and euchromatin (48). In contrast to ...
... Chromatin structure plays an important role in the regulation of gene expression in the eukaryotic cell. Cytological studies have long suggested that different domains of the genome are packaged into two structurally different types of chromatin, heterochromatin and euchromatin (48). In contrast to ...
Eds., Y. Murakami, K. Nakayama, S.-I. Kitamura, H. Iwata and... © by TERRAPUB, 2008.
... The TC-resistant Vibrio and L. garvieae containing tet(M) gene were used for transfer experiment of TC resistance to E. coli and E. faecalis by filter-mating and filter-separating methods. As shown in Table 1, transformation rate in filter mating of E. coli was 10 –3 when Vibrio was donor, whereas t ...
... The TC-resistant Vibrio and L. garvieae containing tet(M) gene were used for transfer experiment of TC resistance to E. coli and E. faecalis by filter-mating and filter-separating methods. As shown in Table 1, transformation rate in filter mating of E. coli was 10 –3 when Vibrio was donor, whereas t ...
srep09383-s1
... likely due to unintended recombination events in Bacillus. Thus, self-replicable plasmid carrying huge DNA fragment was realized to be unstable. To overcome the size issue, a new integrative capture vector pCAPB2 was used. pCAPB2-ami was introduced into chromosome of B. subtilis JH642+sfp through na ...
... likely due to unintended recombination events in Bacillus. Thus, self-replicable plasmid carrying huge DNA fragment was realized to be unstable. To overcome the size issue, a new integrative capture vector pCAPB2 was used. pCAPB2-ami was introduced into chromosome of B. subtilis JH642+sfp through na ...
Putrescine oxidase of Micrococcus rubens : primary
... containing 0.1 YO SDS. A distinct 4.4 kb band hybridizing to three probes was detected. From an agarose gel slice (10 x 3 x 2 mm), which contained BamHI fragments in the size range 4 . 2 4 5 kb, DNA was extracted by the sodium perchlorate method (Chen & Thomas, 1980) and ligated with T4 DNA ligase t ...
... containing 0.1 YO SDS. A distinct 4.4 kb band hybridizing to three probes was detected. From an agarose gel slice (10 x 3 x 2 mm), which contained BamHI fragments in the size range 4 . 2 4 5 kb, DNA was extracted by the sodium perchlorate method (Chen & Thomas, 1980) and ligated with T4 DNA ligase t ...
vectors
... This greatly reduces the intracellular copy number of the circular, double-stranded replicative form of the viral DNA (RF), without greatly reducing phage yield. As a result of their reduced copy number, fd-tet mutants that are completely defective for assembly nevertheless can be propagated, wherea ...
... This greatly reduces the intracellular copy number of the circular, double-stranded replicative form of the viral DNA (RF), without greatly reducing phage yield. As a result of their reduced copy number, fd-tet mutants that are completely defective for assembly nevertheless can be propagated, wherea ...
Analysis of a Rhizobium leguminosarum gene
... approximately 5 kb upstream of the previously identified feuP gene (Yeoman et al., 1997). This fragment was cloned into pUC18 to form pBI0860. Various restriction fragments from pBI0860 were subcloned into pUC18 and their sequences were determined. The sequence of the entire 3.7 kb fragment was then ...
... approximately 5 kb upstream of the previously identified feuP gene (Yeoman et al., 1997). This fragment was cloned into pUC18 to form pBI0860. Various restriction fragments from pBI0860 were subcloned into pUC18 and their sequences were determined. The sequence of the entire 3.7 kb fragment was then ...
manual HiScribe T7 In Vitro Transcription Kit E2030
... marker DNA. The presence of small amounts (< 5%) of undigested plasmid will have little effect on RNA yield. ...
... marker DNA. The presence of small amounts (< 5%) of undigested plasmid will have little effect on RNA yield. ...
07-050sr (Microsoft Word
... non-vector system, for Schedule 2, has the meaning given in Part 3 of that Schedule; nucleic acid means either, or both, deoxyribonucleic acid (DNA), or ribonucleic acid (RNA), of any length; oncogenic modification means a genetic modification that is capable of inducing unregulated cell proliferati ...
... non-vector system, for Schedule 2, has the meaning given in Part 3 of that Schedule; nucleic acid means either, or both, deoxyribonucleic acid (DNA), or ribonucleic acid (RNA), of any length; oncogenic modification means a genetic modification that is capable of inducing unregulated cell proliferati ...
The Bacillus subtilis clpC operon encodes DNA
... ClpC of Bacillus subtilis, controlling competence gene expression and survival under stress conditions, is encoded by the fourth gene of a six-gene operon. The product of orfl contains a potential helix-turn-helix motif, but shows no significant similarities with known protein sequences. The second ...
... ClpC of Bacillus subtilis, controlling competence gene expression and survival under stress conditions, is encoded by the fourth gene of a six-gene operon. The product of orfl contains a potential helix-turn-helix motif, but shows no significant similarities with known protein sequences. The second ...
RECOMBINEERING: A POWERFUL NEW TOOL FOR MOUSE
... generates a circular plasmid by gap repair that can replicate and be selected in E. coli. This cloning method removes the need for enzymatic treatment of the PCR product or for in vitro ligation. Unfortunately, in all of these examples, recombination is restricted to special RecBCD-deficient strains ...
... generates a circular plasmid by gap repair that can replicate and be selected in E. coli. This cloning method removes the need for enzymatic treatment of the PCR product or for in vitro ligation. Unfortunately, in all of these examples, recombination is restricted to special RecBCD-deficient strains ...
SEC62 Encodes a Putative Membrane Protein Required for Protein
... 1987) was kindly provided by Linda Silveira (Division of Biochemistry and Molecular Biology, University of California, Berkeley). Escherichia coil plasmid pUCII9 (Vieira and Messing, 1987), and the E. coil-yeast shuttle plasmids YEp351, YIp351 (Hill et ai., 1986), and YEp24 (Botstein et al., 1979) h ...
... 1987) was kindly provided by Linda Silveira (Division of Biochemistry and Molecular Biology, University of California, Berkeley). Escherichia coil plasmid pUCII9 (Vieira and Messing, 1987), and the E. coil-yeast shuttle plasmids YEp351, YIp351 (Hill et ai., 1986), and YEp24 (Botstein et al., 1979) h ...
Chapter 4. Studying DNA Learning outcomes 4.1. Enzymes for DNA
... work if the 5′ ends of the new polynucleotides are shortened in any way. In particular, DNA sequencing is based on synthesis of new polynucleotides, all of which share exactly the same 5′ end, marked by the primer used to initiate the sequencing reactions. If any nibbling of the 5′ ends occurs, then ...
... work if the 5′ ends of the new polynucleotides are shortened in any way. In particular, DNA sequencing is based on synthesis of new polynucleotides, all of which share exactly the same 5′ end, marked by the primer used to initiate the sequencing reactions. If any nibbling of the 5′ ends occurs, then ...
Isolation of insertion elements from Gram
... in question into E. coli and by testing for sucrose sensitivity (data not shown). Therefore, besides mutation of the sacB gene, at least one other, so far unknown resistance mechanism should exist. Since in E. coli the lethal effect parallels with an active levan sucrase in the periplasm [2], chromo ...
... in question into E. coli and by testing for sucrose sensitivity (data not shown). Therefore, besides mutation of the sacB gene, at least one other, so far unknown resistance mechanism should exist. Since in E. coli the lethal effect parallels with an active levan sucrase in the periplasm [2], chromo ...
Plasmid
A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.