Salmonella
... Extra-intestinal infections caused by non-typhoid Salmonella • Non-typhoid Salmonella entrocolitis is complicated by bacteremia (sometimes transient) in approximately 5% of patients overall, and more frequently in infants. • Interestingly, among the >2,500 serotypes of nontyphoid Salmonella, a few, ...
... Extra-intestinal infections caused by non-typhoid Salmonella • Non-typhoid Salmonella entrocolitis is complicated by bacteremia (sometimes transient) in approximately 5% of patients overall, and more frequently in infants. • Interestingly, among the >2,500 serotypes of nontyphoid Salmonella, a few, ...
SPT3 interacts with TFIID to allow normal transcription in
... purifies as a single polypeptide with the same molecular mass as predicted by the sequence of the yeast TFIIDencoding gene (Buratowski et al. 1988; Cavallini et al. 1989; Horikoshi et al. 1989). This result suggests either that yeast TFIID, unlike TFIID from higher eukaryotes, does not associate wit ...
... purifies as a single polypeptide with the same molecular mass as predicted by the sequence of the yeast TFIIDencoding gene (Buratowski et al. 1988; Cavallini et al. 1989; Horikoshi et al. 1989). This result suggests either that yeast TFIID, unlike TFIID from higher eukaryotes, does not associate wit ...
Chromosomes in Saccharomyces cerevisiae
... previously unidentified specialized sequences that are required for accurate chromosome segregation. We examined the role of telomere-centromere separation by analyzing the behavior of telocentric derivatives of chromosome III. We made a full-length, telocentric chromosome by directing a linear plas ...
... previously unidentified specialized sequences that are required for accurate chromosome segregation. We examined the role of telomere-centromere separation by analyzing the behavior of telocentric derivatives of chromosome III. We made a full-length, telocentric chromosome by directing a linear plas ...
Molecular Diagnostics in Clinical Microbiology
... forward. Nucleic acid from the potential pathogen is extracted from the clinical sample, subsequently followed by an amplification-detection protocol, preferably in real-time format, in a single or multiplex assay. However, this simple workflow is punctuated with a number of issues. Many effective s ...
... forward. Nucleic acid from the potential pathogen is extracted from the clinical sample, subsequently followed by an amplification-detection protocol, preferably in real-time format, in a single or multiplex assay. However, this simple workflow is punctuated with a number of issues. Many effective s ...
Mating-Type Genes From the Homothallic Fungus Sordaria
... that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region is shown in Figure 1. DNA sequence analysis: To ...
... that the cloned DNAs are not rearranged with respect to their true genomic organization and that the S. macrospora mating-type genes all have a single copy in the genome (data not shown). The deduced physical map of the genome in the mating-type region is shown in Figure 1. DNA sequence analysis: To ...
The full-length HIV-1 molecular clone pLAI 61 was used to produce
... capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; rev exon 1 with primers NotI-WdV001 and WdV002; rev exon 2 with primers WdV003 and N ...
... capped G residue. Supplementary Table 1 lists all oligonucleotides used in this study. The tat exon 1 was amplified by PCR on pLAI with primers NotI-WdV005 and WdV002; tat exon 2 with primers WdV007 and NotI-WdV004; rev exon 1 with primers NotI-WdV001 and WdV002; rev exon 2 with primers WdV003 and N ...
PDF - Journal of Genomics
... acids and carbohydrates that are abundant in their phloem-based diet or produced by the host. Genomic evidence suggests that several amino acid biosynthetic pathways are shared between aphid and Buchnera, providing the aphid the ability to regulate the endosymbiont’s metabolism (16). No aphid specie ...
... acids and carbohydrates that are abundant in their phloem-based diet or produced by the host. Genomic evidence suggests that several amino acid biosynthetic pathways are shared between aphid and Buchnera, providing the aphid the ability to regulate the endosymbiont’s metabolism (16). No aphid specie ...
Odyssey of Agrobacterium T-DNA.
... mechanism of DNA integration (Offringa et al., 1990; Paszkowski et al., 1988). Illegitimate recombination of T-DNA in the plant genome has been described a decade ago (Gheysen et al., 1991; Matsumoto et al., 1990; Mayerhofer et al., 1991), but little is known about factors involved in this process. ...
... mechanism of DNA integration (Offringa et al., 1990; Paszkowski et al., 1988). Illegitimate recombination of T-DNA in the plant genome has been described a decade ago (Gheysen et al., 1991; Matsumoto et al., 1990; Mayerhofer et al., 1991), but little is known about factors involved in this process. ...
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... any associated heterologous gene. As long as the D., [1987] Molecular Biology. Jones and Bartlett Publish allolactose concentration in the cell is above 104M, ers, Inc, Portola Valley, CA; Beckwith, supra). It was calculated that greater than 20% of the lactose acted 35 the tac promoter provides hig ...
... any associated heterologous gene. As long as the D., [1987] Molecular Biology. Jones and Bartlett Publish allolactose concentration in the cell is above 104M, ers, Inc, Portola Valley, CA; Beckwith, supra). It was calculated that greater than 20% of the lactose acted 35 the tac promoter provides hig ...
Rapid generation of nested chromosomal
... large 11-centiMorgan (cM) deletion and assigned into complementation groups (8). The deletions were used to map the induced mutations (8). Deletion endpoints were cloned and then used for positional cloning of genes (3). One of the genes cloned was eed, the mouse homologue of Drosophila extra sex-co ...
... large 11-centiMorgan (cM) deletion and assigned into complementation groups (8). The deletions were used to map the induced mutations (8). Deletion endpoints were cloned and then used for positional cloning of genes (3). One of the genes cloned was eed, the mouse homologue of Drosophila extra sex-co ...
ADP Ribosylation Factor is an Essential Protein in Saccharomyces cerevisiae and is Encoded by Two Genes.
... genes occupy map positions distinct from all other mapped yeast genes (23) and by that criterion represent newly identified genes. At least one functional ARF gene is required for viability. As a first step in determining the function of the two ARF genes, we constructed deletion-insertion mutations ...
... genes occupy map positions distinct from all other mapped yeast genes (23) and by that criterion represent newly identified genes. At least one functional ARF gene is required for viability. As a first step in determining the function of the two ARF genes, we constructed deletion-insertion mutations ...
Electrophoresis Revised
... • Place the lid on the gel box, connecting the electrodes. • Connect the electrode wires to the power supply, making sure the positive (red) and negative (black) are correctly connected. (Remember – “Run to Red”) • Turn on the power supply to about 100 volts. Maximum allowed voltage will vary depend ...
... • Place the lid on the gel box, connecting the electrodes. • Connect the electrode wires to the power supply, making sure the positive (red) and negative (black) are correctly connected. (Remember – “Run to Red”) • Turn on the power supply to about 100 volts. Maximum allowed voltage will vary depend ...
Introduction to Gel Electrophorsis
... • The voltage applied to the gel affects how quickly the gel runs • The higher the voltage, the more quickly the gel runs………But that often reduces the quality of the DNA separation • >>>>>>>>>>It also generates heat which reduces the quality of the DNA separation ...
... • The voltage applied to the gel affects how quickly the gel runs • The higher the voltage, the more quickly the gel runs………But that often reduces the quality of the DNA separation • >>>>>>>>>>It also generates heat which reduces the quality of the DNA separation ...
Agarose Gel Electrophoresis
... DNA fragments should be considered. Between 5 and 200 ng of a single DNA fragment can be loaded into a 0.5-cm-wide × 0.2-cmdeep sample well; 5 ng approaches the minimal amount of an individual DNA fragment that can be detected by ethidium bromide staining, and 200 ng approximates the most that can b ...
... DNA fragments should be considered. Between 5 and 200 ng of a single DNA fragment can be loaded into a 0.5-cm-wide × 0.2-cmdeep sample well; 5 ng approaches the minimal amount of an individual DNA fragment that can be detected by ethidium bromide staining, and 200 ng approximates the most that can b ...
Elsevier Editorial System(tm) for Diagnostic Microbiology and
... E. miricola was isolated for the first time in 2003 from the condensation water on the space station Mir (Li et al., 2003). The first case of infection due to E. miricola (pneumonia and sepsis) was described in 2008 in a man with lymphoma and under mechanical ventilator support (Green et al., 2008). ...
... E. miricola was isolated for the first time in 2003 from the condensation water on the space station Mir (Li et al., 2003). The first case of infection due to E. miricola (pneumonia and sepsis) was described in 2008 in a man with lymphoma and under mechanical ventilator support (Green et al., 2008). ...
Isolation and characterization of a repeated sequence (RPS1) of
... were virtually identical; however, one or two chromosomes were variable in size (Asakura et al., 1991). In virtually all cases, the chromosome that varied in size was chromosome 2. This suggested that chromosome 2 is too variable to be useful for distinguishing between strains. A similar variable ch ...
... were virtually identical; however, one or two chromosomes were variable in size (Asakura et al., 1991). In virtually all cases, the chromosome that varied in size was chromosome 2. This suggested that chromosome 2 is too variable to be useful for distinguishing between strains. A similar variable ch ...
Deconstructing the Genome: DNA at High Resolution
... released from the nuclei of cells at specific sites. These welldefined cuts generate fragments suitable for manipulation and characterization. A restriction enzyme recognizes a specific sequence of bases anywhere within the genome and then severs two covalent bonds (one in each strand) in the sugar- ...
... released from the nuclei of cells at specific sites. These welldefined cuts generate fragments suitable for manipulation and characterization. A restriction enzyme recognizes a specific sequence of bases anywhere within the genome and then severs two covalent bonds (one in each strand) in the sugar- ...
Agarose Gel Electrophoresis
... While this has a slight effect on the mobility of the DNA (Fig. 10.4.2D), it eliminates the need to stain the gel upon completion of the separation. An added advantage to running gels with ethidium bromide is that the mobility of the DNA can be monitored throughout the run until the desired separati ...
... While this has a slight effect on the mobility of the DNA (Fig. 10.4.2D), it eliminates the need to stain the gel upon completion of the separation. An added advantage to running gels with ethidium bromide is that the mobility of the DNA can be monitored throughout the run until the desired separati ...
A novel locus of Yersinia enterocolitica serotype O:3 involved in
... With this new information available, we wondered whether we could see trans-complementation of phage ~R1-37 receptor by the trs locus in rough derivatives of the mutants. To test this, rough derivatives of previously constructed trans-complementation strains were selected using phage ~YeO3-12 as abo ...
... With this new information available, we wondered whether we could see trans-complementation of phage ~R1-37 receptor by the trs locus in rough derivatives of the mutants. To test this, rough derivatives of previously constructed trans-complementation strains were selected using phage ~YeO3-12 as abo ...
Lab: Colony PCR amplification of the 16S ribosomal RNA gene I
... Microbial community structure is a critical determinant of the biogeochemical processes occurring within the pelagic marine ecosystem. Due to the difficulties of cultivating many marine microbes, microbial ecologists frequently employ molecular biology-based techniques to reveal the complexity of aq ...
... Microbial community structure is a critical determinant of the biogeochemical processes occurring within the pelagic marine ecosystem. Due to the difficulties of cultivating many marine microbes, microbial ecologists frequently employ molecular biology-based techniques to reveal the complexity of aq ...
CHAPTER 14 DNA applications in society
... produce sufficient growth hormone for use, very large numbers of pituitary glands from cadavers were required. Each batch of hGH was derived from a pool of pituitary glands from about 16 000 cadavers. During a year, patients received their hGH injections from two or three different batches. (A batc ...
... produce sufficient growth hormone for use, very large numbers of pituitary glands from cadavers were required. Each batch of hGH was derived from a pool of pituitary glands from about 16 000 cadavers. During a year, patients received their hGH injections from two or three different batches. (A batc ...
Supplementary Table 1: WormBase IDs and given
... were collected onto fresh plates. Some of these animals were only transiently transgenenic and produced exclusively uncoordinated (unc-119) progeny. Only one stably transgenenic line, ...
... were collected onto fresh plates. Some of these animals were only transiently transgenenic and produced exclusively uncoordinated (unc-119) progeny. Only one stably transgenenic line, ...
Biotechnology/Cloning poster - SPARK: Scholarship at Parkland
... make products, or "any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use. Depending on the tools and applications, it often overlaps with the (related) fields of bioengineering, biomedical engine ...
... make products, or "any technological application that uses biological systems, living organisms, or derivatives thereof, to make or modify products or processes for specific use. Depending on the tools and applications, it often overlaps with the (related) fields of bioengineering, biomedical engine ...
Plasmid
A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.