Chapter 1. Introduction 1. Introduction 1.1 Peptidyl
... obtained for Cdc25c and its upstream regulator, polo-like kinase (Plk1) (Crenshow, et al., 1998; Shen, et al., 1998). Cdc25c is a Cdc2-directed phosphatase, a multiple phosphorylated protein during the G2/M transition of the cell cycle. Both, mitotic phosphorylated Cdc25c from Xenopus egg extracts a ...
... obtained for Cdc25c and its upstream regulator, polo-like kinase (Plk1) (Crenshow, et al., 1998; Shen, et al., 1998). Cdc25c is a Cdc2-directed phosphatase, a multiple phosphorylated protein during the G2/M transition of the cell cycle. Both, mitotic phosphorylated Cdc25c from Xenopus egg extracts a ...
RBTopic3_6 Enzymes - wfs
... this, the reactions may occur faster. Enzymes are organic catalysts. They are proteins. 2. The amino acids that make up these enzymes allow a tertiary and/or quaternary structure. Because each enzyme has a specific amino acid sequence, enzymes have a specific three-dimensional shape. 3. The molecule ...
... this, the reactions may occur faster. Enzymes are organic catalysts. They are proteins. 2. The amino acids that make up these enzymes allow a tertiary and/or quaternary structure. Because each enzyme has a specific amino acid sequence, enzymes have a specific three-dimensional shape. 3. The molecule ...
Characteristics of enzymes
... • Enzymes speed up a chemical reaction by lowering the activation energy needed to start the reaction. ...
... • Enzymes speed up a chemical reaction by lowering the activation energy needed to start the reaction. ...
Interaction of small* molecules with membranes.
... ¾ Presence of weakly acidic contaminants (e.g. fatty acids) which act as proton carriers at physiological pH; But: does not account for all anomalous H+ flux ¾ In real systems - protein pumps; But: incorporation of such proteins on vesicles only weakly changes the proton permeability ...
... ¾ Presence of weakly acidic contaminants (e.g. fatty acids) which act as proton carriers at physiological pH; But: does not account for all anomalous H+ flux ¾ In real systems - protein pumps; But: incorporation of such proteins on vesicles only weakly changes the proton permeability ...
doc Syllabus 201
... Lecture 7. The tricarboxylic acid (TCA) cycle. January 18. TCA cycle reactions and energetics Readings: Berg et al., pp. 473-478. Lecture 8. Regulation of the TCA cycle. January 20. The central role of the TCA cycle in metabolism Allosteric regulation of the TCA cycle Readings: Berg et al., pp ...
... Lecture 7. The tricarboxylic acid (TCA) cycle. January 18. TCA cycle reactions and energetics Readings: Berg et al., pp. 473-478. Lecture 8. Regulation of the TCA cycle. January 20. The central role of the TCA cycle in metabolism Allosteric regulation of the TCA cycle Readings: Berg et al., pp ...
Biology 177-201B
... Lecture 7. The tricarboxylic acid (TCA) cycle. January 18. TCA cycle reactions and energetics Readings: Berg et al., pp. 473-478. Lecture 8. Regulation of the TCA cycle. January 20. The central role of the TCA cycle in metabolism Allosteric regulation of the TCA cycle Readings: Berg et al., pp ...
... Lecture 7. The tricarboxylic acid (TCA) cycle. January 18. TCA cycle reactions and energetics Readings: Berg et al., pp. 473-478. Lecture 8. Regulation of the TCA cycle. January 20. The central role of the TCA cycle in metabolism Allosteric regulation of the TCA cycle Readings: Berg et al., pp ...
doc BIOL210syllabus
... Lecture 7. The tricarboxylic acid (TCA) cycle. January 18. TCA cycle reactions and energetics Readings: Berg et al., pp. 473-478. Lecture 8. Regulation of the TCA cycle. January 20. The central role of the TCA cycle in metabolism Allosteric regulation of the TCA cycle Readings: Berg et al., pp ...
... Lecture 7. The tricarboxylic acid (TCA) cycle. January 18. TCA cycle reactions and energetics Readings: Berg et al., pp. 473-478. Lecture 8. Regulation of the TCA cycle. January 20. The central role of the TCA cycle in metabolism Allosteric regulation of the TCA cycle Readings: Berg et al., pp ...
supplementary text
... strength, Imin [data not shown]). Increased density of cliques at various regions within a protein ascertains the strong connectivity in those areas. Unique cliques (k=3) were prevalent in dense clusters in TdT-short isoform, but cliques in TdT-long form were sparsely distributed. Moreover, unlike ...
... strength, Imin [data not shown]). Increased density of cliques at various regions within a protein ascertains the strong connectivity in those areas. Unique cliques (k=3) were prevalent in dense clusters in TdT-short isoform, but cliques in TdT-long form were sparsely distributed. Moreover, unlike ...
ATP BCH 341
... ATP is a nucleotide. It consists of adenine (6 amino derivative of purine, the 5-carbon sugar,ribose, attached to adenine through glucosyl linkage and 3 phosphate group joined to 5‘-position of ribose. ATP is high energy compound because it has a large negative free energy of hydrolysis. ...
... ATP is a nucleotide. It consists of adenine (6 amino derivative of purine, the 5-carbon sugar,ribose, attached to adenine through glucosyl linkage and 3 phosphate group joined to 5‘-position of ribose. ATP is high energy compound because it has a large negative free energy of hydrolysis. ...
Paper
... typical for RTX-toxins and unique by their high number. The repeats are involved in receptor and calcium binding. It has been postulated that each repeat binds a single calcium ion with a binding constant between 0.5-0.8 mM and circular dichroism spectroscopy analysis revealed that calcium binding i ...
... typical for RTX-toxins and unique by their high number. The repeats are involved in receptor and calcium binding. It has been postulated that each repeat binds a single calcium ion with a binding constant between 0.5-0.8 mM and circular dichroism spectroscopy analysis revealed that calcium binding i ...
dialog - the Biology Department
... the geochemical record, such as depletion of inorganic carbonates for 12C and deposits of complex organic carbon enriched in 12C (42). By this time, the oxygenation of the earth was also well under way (9), and it is likely that oxygenic photosynthesis was fully evolved within the domain Bacteria. M ...
... the geochemical record, such as depletion of inorganic carbonates for 12C and deposits of complex organic carbon enriched in 12C (42). By this time, the oxygenation of the earth was also well under way (9), and it is likely that oxygenic photosynthesis was fully evolved within the domain Bacteria. M ...
Poster
... Signal transduction is an essential process in cells. One critical signaling molecule, protein kinase A (PKA), phosphorylates target proteins, thereby changing their conformations and modifying their functions. PKA is a component of multiple signaling pathways that regulate a variety of proteins. Si ...
... Signal transduction is an essential process in cells. One critical signaling molecule, protein kinase A (PKA), phosphorylates target proteins, thereby changing their conformations and modifying their functions. PKA is a component of multiple signaling pathways that regulate a variety of proteins. Si ...
Journal of Bacteriology
... sufficient to produce the desired conformational change. A type 1 metal binding site is also present in CadC as Cys58 and Cys60. However, CadC has an N-terminal extension containing an essential Cys7 and a nonessential Cys11. The addition of this extension is the basis for metal selectivity, with Cy ...
... sufficient to produce the desired conformational change. A type 1 metal binding site is also present in CadC as Cys58 and Cys60. However, CadC has an N-terminal extension containing an essential Cys7 and a nonessential Cys11. The addition of this extension is the basis for metal selectivity, with Cy ...
Enzymes
... Straw is plentiful and it could be used for the production of many organic substances. The first step is the conversion of cellulose to glucose. It has been suggested that an enzyme could be used for this process. There is a difficulty - the lignin that covers the cellulose would protect the cellul ...
... Straw is plentiful and it could be used for the production of many organic substances. The first step is the conversion of cellulose to glucose. It has been suggested that an enzyme could be used for this process. There is a difficulty - the lignin that covers the cellulose would protect the cellul ...
QM/MM Study of Cytochrome P450 BM3
... breaking and making, to be modeled. The large nonreactive part is described by molecular mechanics, and the two regions are able to interact. The combination of the efficiency and speed of the MM force field with the versatility and range of applicability of the QM method allows reactions in large s ...
... breaking and making, to be modeled. The large nonreactive part is described by molecular mechanics, and the two regions are able to interact. The combination of the efficiency and speed of the MM force field with the versatility and range of applicability of the QM method allows reactions in large s ...
Phosphatases - Georgia Institute of Technology
... • GM glycogen targeting subunit of PP1 – GM binds ER, near muscle glycogen stores – GM binds PP1; PP1 near glycogen inactivates phosphorylase, activates GS – Phospho-GM does not bind PP1 ...
... • GM glycogen targeting subunit of PP1 – GM binds ER, near muscle glycogen stores – GM binds PP1; PP1 near glycogen inactivates phosphorylase, activates GS – Phospho-GM does not bind PP1 ...
Protein Function and Classification
... • InterPro is a predictive protein signature database - results are predictions, and should be treated as such • InterPro entries are based on signatures supplied to us by our member databases ....this means no signature, no entry! ...
... • InterPro is a predictive protein signature database - results are predictions, and should be treated as such • InterPro entries are based on signatures supplied to us by our member databases ....this means no signature, no entry! ...
Electron Transport Chain (Respiratory Chain)
... Citrate cycle (CC) and the RCH are interconnected a) by CO2 (produced by CC, used by RCH) b) by NADH (produced by CC, used by RCH) c) an enzyme succinate dehydrogenase d) ATP (produced by RCH, used by CC) ...
... Citrate cycle (CC) and the RCH are interconnected a) by CO2 (produced by CC, used by RCH) b) by NADH (produced by CC, used by RCH) c) an enzyme succinate dehydrogenase d) ATP (produced by RCH, used by CC) ...
enzymes - BEHS Science
... substrate: active site The specificity of an enzyme is due to a compatible fit between the active site and the substrate. (Lock and Key) ...
... substrate: active site The specificity of an enzyme is due to a compatible fit between the active site and the substrate. (Lock and Key) ...
Lecture: 10-14-16
... only with the assistance of a particular protein called a channel. Such movement is called facilitated diffusion or passive transport. Transport proteins function as pumps or channels to facilitate the flow of small molecules across the cell membrane. Movement of molecules against a concentration ...
... only with the assistance of a particular protein called a channel. Such movement is called facilitated diffusion or passive transport. Transport proteins function as pumps or channels to facilitate the flow of small molecules across the cell membrane. Movement of molecules against a concentration ...
Respiration
... 1) Where do light dependent reactions take place? 2) How are the equations of respiration and photosynthesis similar? 3) List the three parts of ATP. 4) Photosynthesis is to chloroplasts as cell respiration is to_____________. 5) Where on the ATP molecule is a bond broken to make ADP? ...
... 1) Where do light dependent reactions take place? 2) How are the equations of respiration and photosynthesis similar? 3) List the three parts of ATP. 4) Photosynthesis is to chloroplasts as cell respiration is to_____________. 5) Where on the ATP molecule is a bond broken to make ADP? ...
Membranes - Active Transport (GPC)
... How does the sodium-potassium pump make the interior of the cell negatively charged? a. by expelling anions b. by pulling in anions c. by expelling more cations than are taken in d. by taking in and expelling an equal number of cations What is the combination of an electrical gradient and a concentr ...
... How does the sodium-potassium pump make the interior of the cell negatively charged? a. by expelling anions b. by pulling in anions c. by expelling more cations than are taken in d. by taking in and expelling an equal number of cations What is the combination of an electrical gradient and a concentr ...
Molecular dynamics simulations of membrane
... No specific knowledge is required for this laboratory practice. THEORETICAL BACKGROUND The application of high electric fields to cells or tissues permeabilizes the cell membrane and is thought to produce aqueous-filled pores in the lipid bilayer. Electroporation is witnessed when the lipid membrane ...
... No specific knowledge is required for this laboratory practice. THEORETICAL BACKGROUND The application of high electric fields to cells or tissues permeabilizes the cell membrane and is thought to produce aqueous-filled pores in the lipid bilayer. Electroporation is witnessed when the lipid membrane ...
P-type ATPase
The P-type ATPases, also known as E1-E2 ATPases, are a large group of evolutionarily related ion and lipid pumps that are found in bacteria, archaea, and eukaryotes. They are α-helical bundle primary transporters referred to as P-type ATPases because they catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump. In addition, they all appear to interconvert between at least two different conformations, denoted by E1 and E2.Most members of this transporter family are specific for the pumping of a large array of cations, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.Prominent examples of P-type ATPases are the sodium-potassium pump (Na+,K+-ATPase), the plasma membrane proton pump (H+-ATPase), the proton-potassium pump (H+,K+-ATPase), and the calcium pump (Ca2+-ATPase).