More Info - Genoprice
... Recombinant Human Long-R3-IGF-I produced in E. coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9111 Dalton. Quality Control: Biological activity: The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells is less ...
... Recombinant Human Long-R3-IGF-I produced in E. coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9111 Dalton. Quality Control: Biological activity: The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells is less ...
slide - KOCSEA
... Compares each output cluster X with the real functional annotation Y (from MIPS) Recall = (# of common proteins of X and Y) / (# of proteins in Y) Precision = (# of common proteins of X and Y) / (# of proteins in X) f-measure = 2 × Recall × Precision / (Recall + Precision) ...
... Compares each output cluster X with the real functional annotation Y (from MIPS) Recall = (# of common proteins of X and Y) / (# of proteins in Y) Precision = (# of common proteins of X and Y) / (# of proteins in X) f-measure = 2 × Recall × Precision / (Recall + Precision) ...
light
... that downstream events may occur in the nucleus But things are more complicated—studies show that both cytosolic and nuclear pathways exist ...
... that downstream events may occur in the nucleus But things are more complicated—studies show that both cytosolic and nuclear pathways exist ...
Organisms are relatively similar at a molecular level
... Please provide your answer on the separate sheet. The overall 3-D shape that this polypeptide folds up into is an example of what kind of structure? Please provide your answer on the separate sheet. Just about every organism known has cytochrome C, and its function is exactly the same in every organ ...
... Please provide your answer on the separate sheet. The overall 3-D shape that this polypeptide folds up into is an example of what kind of structure? Please provide your answer on the separate sheet. Just about every organism known has cytochrome C, and its function is exactly the same in every organ ...
Leah Cooper
... requirement for the 9 essential amino acids (EAA) because our body’s inability to synthesize them. However, it is of importance to note that the requirement for total nitrogen is the primary determinant of total protein requirement, which includes a need for both EAA and NEAA. There is a dietary nit ...
... requirement for the 9 essential amino acids (EAA) because our body’s inability to synthesize them. However, it is of importance to note that the requirement for total nitrogen is the primary determinant of total protein requirement, which includes a need for both EAA and NEAA. There is a dietary nit ...
Thermodynamics of Protein Folding
... • Steitz et al. suggest Taq has 4 additional internal H-bonds and 2 additional ion/ion interactions compared to Klenow • Waksman et al. suggest fewer unfavorable electrostatic charges lead to global rearrangement of electrostatic distribution and more buried nonpolar space • LiCata suggests that unf ...
... • Steitz et al. suggest Taq has 4 additional internal H-bonds and 2 additional ion/ion interactions compared to Klenow • Waksman et al. suggest fewer unfavorable electrostatic charges lead to global rearrangement of electrostatic distribution and more buried nonpolar space • LiCata suggests that unf ...
Glycosylation of Proteins - Structure, Function and Analysis
... Mass spectrometry can again be used for this type of analysis in conjunction with a multistep chemical hydrolysis and derivatisation process. The end result of the chemistry is that glycans are converted to their constituent monosaccharides containing chemical labels on unbound hydroxyl groups and d ...
... Mass spectrometry can again be used for this type of analysis in conjunction with a multistep chemical hydrolysis and derivatisation process. The end result of the chemistry is that glycans are converted to their constituent monosaccharides containing chemical labels on unbound hydroxyl groups and d ...
Protein visualization
... The easiest way to present a protein structure is to build a 3D model of it. Visualization programs allow user to view and rotate the model and change the model structure to find the desirable information from it. Common way to visualize proteins are building a wire framework of the amino acid chain ...
... The easiest way to present a protein structure is to build a 3D model of it. Visualization programs allow user to view and rotate the model and change the model structure to find the desirable information from it. Common way to visualize proteins are building a wire framework of the amino acid chain ...
C.N.R. Short-term Mobility Program 2008 Gabriella Pocsfalvi
... carbamidomethyl, variable modifications: oxidation on methionie, automatic error tolerant search; peptide tolerance 50 ppm, MS/MS tolerance 0.08 Da. SELDI-TOFMS: SELDI-TOF mass spectra were obtained using PCS-4000 (BioRad) linear LDI instrument on the complex protein samples without separation and p ...
... carbamidomethyl, variable modifications: oxidation on methionie, automatic error tolerant search; peptide tolerance 50 ppm, MS/MS tolerance 0.08 Da. SELDI-TOFMS: SELDI-TOF mass spectra were obtained using PCS-4000 (BioRad) linear LDI instrument on the complex protein samples without separation and p ...
THERAPUETIC DISCOVERY BY MODELLING
... verified inhibitors from a much larger library of 1 million compounds. Use data from experimental studies to figure out when our predicted inhibitors are likely to be cell-active and drug-like in their behaviour; use machine learning approaches to learn from compound characteristics (PK, ADME, toxic ...
... verified inhibitors from a much larger library of 1 million compounds. Use data from experimental studies to figure out when our predicted inhibitors are likely to be cell-active and drug-like in their behaviour; use machine learning approaches to learn from compound characteristics (PK, ADME, toxic ...
6. Appendix: Protein structure
... 6. Appendix: Protein structure The following section contains a very brief introduction to some aspects of protein and peptide structure. It is basically a summary of the description given by Mathews and van Holde in "Biochemistry" and is just intended to help you work through this practical. For mo ...
... 6. Appendix: Protein structure The following section contains a very brief introduction to some aspects of protein and peptide structure. It is basically a summary of the description given by Mathews and van Holde in "Biochemistry" and is just intended to help you work through this practical. For mo ...
Protein structure homework: FAQ
... Protein structure homework: FAQ Q: Did you tell us not to go to molecule and then information in RasTop to find info on the proteins but for us to open the protein files in notepad. A: I did not tell you not to use the information button -- I warned you that it might be misreading and asked you to v ...
... Protein structure homework: FAQ Q: Did you tell us not to go to molecule and then information in RasTop to find info on the proteins but for us to open the protein files in notepad. A: I did not tell you not to use the information button -- I warned you that it might be misreading and asked you to v ...
Z4751 - Datasheet - Sigma
... immunofluorescence. Detection of the zyxin band by immunoblotting is specifically inhibited with the immunizing peptide. ...
... immunofluorescence. Detection of the zyxin band by immunoblotting is specifically inhibited with the immunizing peptide. ...
ANSWER: Proteins, Amino Acids and Carbs
... Ignoring non-GMO soy protein for the moment, when your metabolism breaks down the protein to free up the protein carbohydrates for cellular respiration, the amino acids are also freed and will build up protein tissue within the muscles being exercised. Weight for weight, proteins contain considerabl ...
... Ignoring non-GMO soy protein for the moment, when your metabolism breaks down the protein to free up the protein carbohydrates for cellular respiration, the amino acids are also freed and will build up protein tissue within the muscles being exercised. Weight for weight, proteins contain considerabl ...
doc - DePaul University
... spectrometer where it is analyzed. This ionization and vaporization process can be aided by the introduction of an inert gas such as nitrogen, and by applying heat to the positively charged needle [4]. Two sub-categories of electrospray ionization that have arisen are nanospray and microspray, both ...
... spectrometer where it is analyzed. This ionization and vaporization process can be aided by the introduction of an inert gas such as nitrogen, and by applying heat to the positively charged needle [4]. Two sub-categories of electrospray ionization that have arisen are nanospray and microspray, both ...
an example documented paragraph!!!
... NFPs are the major axonal cytoskeletal components of neurons and are essential for establishing the correct diameters of large myelinated motor and sensory axons (1-3). The NFP assembly is composed of three different polypeptide subunits in the adult neuron: NFL (low molecular mass), NFM (intermedia ...
... NFPs are the major axonal cytoskeletal components of neurons and are essential for establishing the correct diameters of large myelinated motor and sensory axons (1-3). The NFP assembly is composed of three different polypeptide subunits in the adult neuron: NFL (low molecular mass), NFM (intermedia ...
Fulltext PDF - Indian Academy of Sciences
... preprolactin carried a sequence of amino acids that targeted them to the ER mediated ...
... preprolactin carried a sequence of amino acids that targeted them to the ER mediated ...
A simple and efficient method for the purification
... resuspended in coupling buffer consisting of 10 mM Tris pH 8.0, 1 mM EDTA, and 1 M NaCl. 100 mg of oligo dT cellulose (Boehringer Mannheim) was hydrated in the same buffer. The resulting mixture was transferred to a Poly Prep Chromatography Column (Bio Rad) and washed with 15 ml coupling buffer. The ...
... resuspended in coupling buffer consisting of 10 mM Tris pH 8.0, 1 mM EDTA, and 1 M NaCl. 100 mg of oligo dT cellulose (Boehringer Mannheim) was hydrated in the same buffer. The resulting mixture was transferred to a Poly Prep Chromatography Column (Bio Rad) and washed with 15 ml coupling buffer. The ...
Substances required for living processes
... the phospholipid, is a major component of cell membranes. Building Blocks of Proteins Like carbohydrates and lipids, proteins contain the elements carbon (C), hydrogen (H) and oxygen (O), but in addition they also always contain nitrogen (N). Sulphur is often present as well as iron and phosphorus. ...
... the phospholipid, is a major component of cell membranes. Building Blocks of Proteins Like carbohydrates and lipids, proteins contain the elements carbon (C), hydrogen (H) and oxygen (O), but in addition they also always contain nitrogen (N). Sulphur is often present as well as iron and phosphorus. ...
Protein Architecture and Structure Alignment
... Anfinsen’s thermodynamic hypothesis “The three-dimensional structure of a native protein in its normal physiological milieu (solvent, pH, ionic strength, presence of other components such as metal ions or prosthetic groups, temperature, etc.) is the one in which the Gibbs free energy of the whole s ...
... Anfinsen’s thermodynamic hypothesis “The three-dimensional structure of a native protein in its normal physiological milieu (solvent, pH, ionic strength, presence of other components such as metal ions or prosthetic groups, temperature, etc.) is the one in which the Gibbs free energy of the whole s ...
Amino acids, peptides and proteins
... Twenty different residues are involved in protein synthesis. These residues might be modified after the synthesis of the polypeptide chain. The other components of proteins are called prosthetic groups. The structure of the amino acids and their characteristic property as amphoteric molecules is des ...
... Twenty different residues are involved in protein synthesis. These residues might be modified after the synthesis of the polypeptide chain. The other components of proteins are called prosthetic groups. The structure of the amino acids and their characteristic property as amphoteric molecules is des ...
The structural basis of an exeptional protein kinase
... magnesium balance. This is of central importance for various physiological processes. Together with TRPM7, TRPM6 is unique among the ion channel field as it consists of an ion channel merged with a kinase domain. Protein kinases are enzymes that phosphorylate target proteins to modulate their functi ...
... magnesium balance. This is of central importance for various physiological processes. Together with TRPM7, TRPM6 is unique among the ion channel field as it consists of an ion channel merged with a kinase domain. Protein kinases are enzymes that phosphorylate target proteins to modulate their functi ...
Protein Assay
... The RC DC Protein Assay is a colorimetric assay for protein quantification with all the functionality of the original DC Protein Assay. This assay is based on the Lowry1 assay but has been modified to be reducing agent compatible (RC) as well as detergent compatible (DC). Procedures 1. Add 100 µl of ...
... The RC DC Protein Assay is a colorimetric assay for protein quantification with all the functionality of the original DC Protein Assay. This assay is based on the Lowry1 assay but has been modified to be reducing agent compatible (RC) as well as detergent compatible (DC). Procedures 1. Add 100 µl of ...
Protein mass spectrometry
Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an important emerging method for the characterization of proteins. The two primary methods for ionization of whole proteins are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). In keeping with the performance and mass range of available mass spectrometers, two approaches are used for characterizing proteins. In the first, intact proteins are ionized by either of the two techniques described above, and then introduced to a mass analyzer. This approach is referred to as ""top-down"" strategy of protein analysis. In the second, proteins are enzymatically digested into smaller peptides using a protease such as trypsin. Subsequently these peptides are introduced into the mass spectrometer and identified by peptide mass fingerprinting or tandem mass spectrometry. Hence, this latter approach (also called ""bottom-up"" proteomics) uses identification at the peptide level to infer the existence of proteins.Whole protein mass analysis is primarily conducted using either time-of-flight (TOF) MS, or Fourier transform ion cyclotron resonance (FT-ICR). These two types of instrument are preferable here because of their wide mass range, and in the case of FT-ICR, its high mass accuracy. Mass analysis of proteolytic peptides is a much more popular method of protein characterization, as cheaper instrument designs can be used for characterization. Additionally, sample preparation is easier once whole proteins have been digested into smaller peptide fragments. The most widely used instrument for peptide mass analysis are the MALDI time-of-flight instruments as they permit the acquisition of peptide mass fingerprints (PMFs) at high pace (1 PMF can be analyzed in approx. 10 sec). Multiple stage quadrupole-time-of-flight and the quadrupole ion trap also find use in this application.