The lower Vmax is consistent with the mutation resulting in a
... type αCP1 in order to determine Vmax and Km. One would then repeat the experiment at different constant concentrations of point mutant (which cannot be phosphorylated) and observe whether the mutant is a competitive inhibitor. A secondary plot of Km,apparent versus [mutant]o would allow determinatio ...
... type αCP1 in order to determine Vmax and Km. One would then repeat the experiment at different constant concentrations of point mutant (which cannot be phosphorylated) and observe whether the mutant is a competitive inhibitor. A secondary plot of Km,apparent versus [mutant]o would allow determinatio ...
Problem set answers
... The lower Vmax is consistent with the mutation resulting in a decreased kcat, which is what one would expect if Glu45 is a catalytic group in the active site. Therefore, the observed effect on Vmax is consistent with the hypothesis. The effect of the mutation on Km suggest one of two possibilities: ...
... The lower Vmax is consistent with the mutation resulting in a decreased kcat, which is what one would expect if Glu45 is a catalytic group in the active site. Therefore, the observed effect on Vmax is consistent with the hypothesis. The effect of the mutation on Km suggest one of two possibilities: ...
Gene Network Central
... • To see which genes are expressed in a tissue of interest, use the “View Tissue-Specific Association” drop-down menu to view known associations for the tissue of interest. • The image to the right shows associations that have been reported from normal human blood samples. ...
... • To see which genes are expressed in a tissue of interest, use the “View Tissue-Specific Association” drop-down menu to view known associations for the tissue of interest. • The image to the right shows associations that have been reported from normal human blood samples. ...
Chapter 7 Cellular control
... different amino acids, and the sequence in which they are strung together determines the structure – and therefore the function – of the protein molecule that is made. DNA determines this sequence. The sequence of bases in a DNA molecule determines the sequences of amino acids in the proteins that t ...
... different amino acids, and the sequence in which they are strung together determines the structure – and therefore the function – of the protein molecule that is made. DNA determines this sequence. The sequence of bases in a DNA molecule determines the sequences of amino acids in the proteins that t ...
5 carbohydrates and the Krebs Cycle
... Galactose gets phosphorylated like glucose, and its uptake is also insulin-dependent o It reacts with uridine diphosphoglucose to form uridine diphosphogalactose. o If you are defif\cient in the catalyzing enzyme, the galactose accumulates in the bloodstream and causes GALACTOSEMIA which produces se ...
... Galactose gets phosphorylated like glucose, and its uptake is also insulin-dependent o It reacts with uridine diphosphoglucose to form uridine diphosphogalactose. o If you are defif\cient in the catalyzing enzyme, the galactose accumulates in the bloodstream and causes GALACTOSEMIA which produces se ...
Chapter 7
... and production of pyruvate. Referred to as the Embden-meyerhof pathway ( in the cytosol). (2)oxidation of pyruvate: pyruvate produced by glycolysis is transported into the mitosol and is oxidized to CO2 and H2O with production of ...
... and production of pyruvate. Referred to as the Embden-meyerhof pathway ( in the cytosol). (2)oxidation of pyruvate: pyruvate produced by glycolysis is transported into the mitosol and is oxidized to CO2 and H2O with production of ...
Powerpoint for chapters 17-20 of Campbell Biology by Emily Diamond
... the extent of the damage a virus can cause depends on a tissues ability to regenerate. example: common cold vs ...
... the extent of the damage a virus can cause depends on a tissues ability to regenerate. example: common cold vs ...
Discussion Questions for Week 5: HWA Pages 167-177
... 1. Define and briefly describe the major sets of reactions in aerobic catabolism. 2. How are oxidative phrosphorylation and substrate level phosphoylation different? 3. HWA states that, in a very narrow sense, glycolysis and the Kreb’s cycle can proceed without O2. Why, then, is O2 necessary for aer ...
... 1. Define and briefly describe the major sets of reactions in aerobic catabolism. 2. How are oxidative phrosphorylation and substrate level phosphoylation different? 3. HWA states that, in a very narrow sense, glycolysis and the Kreb’s cycle can proceed without O2. Why, then, is O2 necessary for aer ...
Mutation of a Ubiquitously Expressed Mouse Transmembrane
... portion of intron 4, in which it terminates. This cDNA was reported to encode the human cytomegalovirus (HCMV) gH receptor (Baldwin et al. 2000). A BLAST search of the mouse TAPT1 amino acid sequence against human proteins revealed a high similarity to that part of the AF189251 protein encoded by ex ...
... portion of intron 4, in which it terminates. This cDNA was reported to encode the human cytomegalovirus (HCMV) gH receptor (Baldwin et al. 2000). A BLAST search of the mouse TAPT1 amino acid sequence against human proteins revealed a high similarity to that part of the AF189251 protein encoded by ex ...
Theory21_30
... The concentration of glucose 6-phosphate can rise high enough in liver to stimulate glycogen synthase, but this does not happen in muscle A build up of glucose 6-phosphate inhibits further glucose trapping in muscle, but not liver In liver, but not muscle, glucose is only made into glucose 6-phospha ...
... The concentration of glucose 6-phosphate can rise high enough in liver to stimulate glycogen synthase, but this does not happen in muscle A build up of glucose 6-phosphate inhibits further glucose trapping in muscle, but not liver In liver, but not muscle, glucose is only made into glucose 6-phospha ...
Workshop VII Secondary metabolism Chair: Christian Hertweck 161
... and an oxidative break. The dehydrogenations are carried out by a single enzyme, product of the gene carB. Deregulated mutants, affected in the gene carS, are deep orange and accumulate large amounts of carotenoids under any culture conditions. After mutagenesis of a carS strain, we have identified ...
... and an oxidative break. The dehydrogenations are carried out by a single enzyme, product of the gene carB. Deregulated mutants, affected in the gene carS, are deep orange and accumulate large amounts of carotenoids under any culture conditions. After mutagenesis of a carS strain, we have identified ...
PPTX - Bonham Chemistry
... Central Importance of Glucose • Glucose is an excellent fuel – Yields good amount of energy upon oxidation(-2840 kJ/mole) – Can be efficiently stored in the polymeric form – Many organisms and tissues can meet their energy needs on glucose only ...
... Central Importance of Glucose • Glucose is an excellent fuel – Yields good amount of energy upon oxidation(-2840 kJ/mole) – Can be efficiently stored in the polymeric form – Many organisms and tissues can meet their energy needs on glucose only ...
DNA-binding proteins
... • Effectors: collective term for inducers and repressors • Effectors affect transcription indirectly by binding to specific DNA-binding proteins ...
... • Effectors: collective term for inducers and repressors • Effectors affect transcription indirectly by binding to specific DNA-binding proteins ...
digestive complete - Anabolic Laboratories
... Short description of enzyme activities Amylases – enzymes which hydrolyze, break apart, large starches into water soluble, simple sugars such as glucose and galactose. These enzymes are present in both saliva and pancreatic secretions and some require calcium to function. Glucoamylase - another ...
... Short description of enzyme activities Amylases – enzymes which hydrolyze, break apart, large starches into water soluble, simple sugars such as glucose and galactose. These enzymes are present in both saliva and pancreatic secretions and some require calcium to function. Glucoamylase - another ...
2010 PCB 5530 Class Projects
... predictions – but remember that they may be ‘overannotated’ (via homology) as actually being pathway enzymes even though they are not. Note also: - Metabolites, enzymes, and genes have been given various names over the years, and GenBank contains different versions of predictions for the same genes/ ...
... predictions – but remember that they may be ‘overannotated’ (via homology) as actually being pathway enzymes even though they are not. Note also: - Metabolites, enzymes, and genes have been given various names over the years, and GenBank contains different versions of predictions for the same genes/ ...
An operon encoding a novel ABC-type transport
... sugars, amino acids, peptides, anions and metals are transported across the cytoplasmic membrane by the ABC-type (or ATP-binding cassette type) multicomponent transport systems. The common protein components of these systems include two transmembrane proteins that usually span the membrane about six ...
... sugars, amino acids, peptides, anions and metals are transported across the cytoplasmic membrane by the ABC-type (or ATP-binding cassette type) multicomponent transport systems. The common protein components of these systems include two transmembrane proteins that usually span the membrane about six ...
Chapter 7 Molecular Genetics: From DNA to Proteins Worksheets
... Write true if the statement is true or false if the statement is false. _____ 1. For many decades, scientists thought that proteins were the genetic material. _____ 2. In eukaryotic cells, proteins always remain in the nucleus, but DNA is made at ribosomes in the cytoplasm. _____ 3. RNA is much larg ...
... Write true if the statement is true or false if the statement is false. _____ 1. For many decades, scientists thought that proteins were the genetic material. _____ 2. In eukaryotic cells, proteins always remain in the nucleus, but DNA is made at ribosomes in the cytoplasm. _____ 3. RNA is much larg ...
Chapter 8 Homeostasis of blood sugar, gas concentrations and
... Influence of hormones on adrenal glands on blood sugar concentrations ...
... Influence of hormones on adrenal glands on blood sugar concentrations ...
SURVEY OF BIOCHEMISTRY
... In E. coli, the lactose operon consists of 3 structural genes: Beta-Galactosidase (Z) Galactoside Permease (Y) Thiogalactoside Transacetylase (A) ...
... In E. coli, the lactose operon consists of 3 structural genes: Beta-Galactosidase (Z) Galactoside Permease (Y) Thiogalactoside Transacetylase (A) ...
Fate of glucose:
... Insulin is produced in the pancreas in response to high glucose levels Insulin allows for more glucose to be taken up by the cells faster for glycolysis Glucose is converted to glucose-6-phosphate once inside the cells so it can’t leave and glucose keeps coming in because the concentration is always ...
... Insulin is produced in the pancreas in response to high glucose levels Insulin allows for more glucose to be taken up by the cells faster for glycolysis Glucose is converted to glucose-6-phosphate once inside the cells so it can’t leave and glucose keeps coming in because the concentration is always ...
PDF
... tors. The correlation between factors is described by the concurrence frequency, which is defined as the number of genes having both factors’ sites over the total number of genes having at least one factor’s site. Predicted_by is a ternary relationship among putative_site, matrix and consensus_patte ...
... tors. The correlation between factors is described by the concurrence frequency, which is defined as the number of genes having both factors’ sites over the total number of genes having at least one factor’s site. Predicted_by is a ternary relationship among putative_site, matrix and consensus_patte ...
F10 Mutagenesis
... Structural and biochemical characterizations important in the identification of microorganisms require pure cultures. A pure culture theoretically contains a single species of microorganism. There are a number of procedures available for the isolation of pure cultures from mixed populations. A pure ...
... Structural and biochemical characterizations important in the identification of microorganisms require pure cultures. A pure culture theoretically contains a single species of microorganism. There are a number of procedures available for the isolation of pure cultures from mixed populations. A pure ...
Lecture 2 Glycolysis
... Can be split into 2 phases 1. Preparatory or activation (Investment) phase 2. Payoff phase ...
... Can be split into 2 phases 1. Preparatory or activation (Investment) phase 2. Payoff phase ...
Lac operon
lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria. Although glucose is the preferred carbon source for most bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available. Gene regulation of the lac operon was the first genetic regulatory mechanism to be understood clearly, so it has become a foremost example of prokaryotic gene regulation. It is often discussed in introductory molecular and cellular biology classes at universities for this reason.Bacterial operons are polycistronic transcripts that are able to produce multiple proteins from one mRNA transcript. In this case, when lactose is required as a sugar source for the bacterium, the three genes of the lac operon can be expressed and their subsequent proteins translated: lacZ, lacY, and lacA. The gene product of lacZ is β-galactosidase which cleaves lactose, a disaccharide, into glucose and galactose. LacY encodes lactose permease, a protein which becomes embedded in the cytoplasmic membrane to enable transport of lactose into the cell. Finally, lacA encodes galactoside O-acetyltransferase. Layout of the lac operon.It would be wasteful to produce the enzymes when there is no lactose available or if there is a more preferable energy source available, such as glucose. The lac operon uses a two-part control mechanism to ensure that the cell expends energy producing the enzymes encoded by the lac operon only when necessary. In the absence of lactose, the lac repressor halts production of the enzymes encoded by the lac operon. In the presence of glucose, the catabolite activator protein (CAP), required for production of the enzymes, remains inactive, and EIIAGlc shuts down lactose permease to prevent transport of lactose into the cell. This dual control mechanism causes the sequential utilization of glucose and lactose in two distinct growth phases, known as diauxie.