Lecture 26 - Glycolysis 2
... and ADP (low energy charge), whereas, it is allosterically inhibited by ATP and citrate (high energy charge). AMP stabilizes the R form of the enzyme (active), and ATP stabilizes the T form (inactive). The activity of pyruvate kinase is controlled by both phosphorylation and allosteric regulation. ...
... and ADP (low energy charge), whereas, it is allosterically inhibited by ATP and citrate (high energy charge). AMP stabilizes the R form of the enzyme (active), and ATP stabilizes the T form (inactive). The activity of pyruvate kinase is controlled by both phosphorylation and allosteric regulation. ...
Determine the blood glucose level
... value we speak of hypoglycemia; when the level is higher than normal we call it hyperglycemia. Many methods were used for determination of blood glucose. Recently, enzymatic methods, using the highly specific enzyme glucose oxidase, were developed for the routine determination of blood glucose Ievel ...
... value we speak of hypoglycemia; when the level is higher than normal we call it hyperglycemia. Many methods were used for determination of blood glucose. Recently, enzymatic methods, using the highly specific enzyme glucose oxidase, were developed for the routine determination of blood glucose Ievel ...
AP Chapter 5A WS - TJ
... d. in the location of their glycosidic linkage. e. in their structural formulas. 28. Which of the following is not true of cellulose? a. It is the most abundant organic compound on Earth. b. It differs from starch because of the configuration of glucose and the geometry of the glycosidic linkage. c. ...
... d. in the location of their glycosidic linkage. e. in their structural formulas. 28. Which of the following is not true of cellulose? a. It is the most abundant organic compound on Earth. b. It differs from starch because of the configuration of glucose and the geometry of the glycosidic linkage. c. ...
lecture - Berkeley MCB
... cells. The results described here suggest an exactly opposite hypothesis. The facts can be explained by the supposition that the i gene determines (via an enzyme intermediate) the synthesis, not of an inducer, but of a "repressor" which blocks the synthesis of b-galactosidase, and the exogenous indu ...
... cells. The results described here suggest an exactly opposite hypothesis. The facts can be explained by the supposition that the i gene determines (via an enzyme intermediate) the synthesis, not of an inducer, but of a "repressor" which blocks the synthesis of b-galactosidase, and the exogenous indu ...
TARBP2 mediated post-transcriptional regulation of gene
... Role of DICER cofactors during post-transcriptional regulation of gene expression in vivo ...
... Role of DICER cofactors during post-transcriptional regulation of gene expression in vivo ...
POSTER Vp-1
... Aspergillus nidulans is conditionally lethal, since loss-of-function mutations in the acuH gene, encoding the fungal CACT, allow growth on alternative carbon sources to fatty acids, such as carbohydrates or amino acids. In this work we have developed a fungal model for the human CACT deficiency base ...
... Aspergillus nidulans is conditionally lethal, since loss-of-function mutations in the acuH gene, encoding the fungal CACT, allow growth on alternative carbon sources to fatty acids, such as carbohydrates or amino acids. In this work we have developed a fungal model for the human CACT deficiency base ...
Graph Drawing
... #Tokens in a place denote the expression level Transitions are the various modi of the gene (inhibited, activated, basal, etc.) ...
... #Tokens in a place denote the expression level Transitions are the various modi of the gene (inhibited, activated, basal, etc.) ...
Red slant with yellow butt
... yellow and stay yellow. However, if only glucose is used, even though the butt turns yellow only after a few hours it will revert to red because the protein in the medium is broken down to alkaline products when the small amount of glucose is used up. • If lactose and/or sucrose are used, the large ...
... yellow and stay yellow. However, if only glucose is used, even though the butt turns yellow only after a few hours it will revert to red because the protein in the medium is broken down to alkaline products when the small amount of glucose is used up. • If lactose and/or sucrose are used, the large ...
(Vibrcgen-Zellmiihle, Fa. E. Buhl er, Tiibingen, Germany). The results
... Eater, W . K . G l y c e r o l ...
... Eater, W . K . G l y c e r o l ...
Ch7 microbgeneticspart1HOLrg
... Synthesis of the lagging strand must be reinitiated as more template is exposed. Each time synthesis is reinitiated, a new RNA primer must be made. Discontinuous synthesis ...
... Synthesis of the lagging strand must be reinitiated as more template is exposed. Each time synthesis is reinitiated, a new RNA primer must be made. Discontinuous synthesis ...
Substrate Breakdown
... Glycogen use by a specific skeletal muscle during exercise is limited to its own reserves It cannot borrow from other muscles although glycogen levels in them may decrease due to the catabolic influence of catecholamines ...
... Glycogen use by a specific skeletal muscle during exercise is limited to its own reserves It cannot borrow from other muscles although glycogen levels in them may decrease due to the catabolic influence of catecholamines ...
Regulation of Heat-Shock Response in Bacteria
... These regulatory elements will be reviewed looking at the groE and dnaK operons. These operons, coding for the bacterial equivalent of Hsp10+60 and Hsp70, respectively, contain in many bacteria a conserved regulatory inverted repeat (IR=CIRCE), and are transcribed either by the vegetative sigma fact ...
... These regulatory elements will be reviewed looking at the groE and dnaK operons. These operons, coding for the bacterial equivalent of Hsp10+60 and Hsp70, respectively, contain in many bacteria a conserved regulatory inverted repeat (IR=CIRCE), and are transcribed either by the vegetative sigma fact ...
Supplement Material…
... in fresh CM or DSM [2] to an OD600 of 2.0. The cultures were then diluted 20-fold in fresh media supplemented with IPTG and synthetic PhrF (LifeTein) where indicated. 200 l were dispensed per well in duplicate in a 96-well black plate (Corning). Luciferin was added to each well at a final concentr ...
... in fresh CM or DSM [2] to an OD600 of 2.0. The cultures were then diluted 20-fold in fresh media supplemented with IPTG and synthetic PhrF (LifeTein) where indicated. 200 l were dispensed per well in duplicate in a 96-well black plate (Corning). Luciferin was added to each well at a final concentr ...
Molecular Modeling Activity for Carbohydrates
... In order to join the molecules, remove an OH end from one molecule and an -H end from another. 7. Does removing the -H and OH ends allow the molecules to fit easily together? ___________________ 8. The -H and OH ends that were removed can also fit together with each other to form a molecule. Thi ...
... In order to join the molecules, remove an OH end from one molecule and an -H end from another. 7. Does removing the -H and OH ends allow the molecules to fit easily together? ___________________ 8. The -H and OH ends that were removed can also fit together with each other to form a molecule. Thi ...
Document
... Some precursors are produced in mitochondria Takes place in liver and kidney Synthesis of glucose or glycogen from non carbohydrates like pyruvate, lactate glucogenic amino acids, glycerol and propionic acid Pathway involves steps of TCA cycle and reversal of glycolysis The irreversible steps o ...
... Some precursors are produced in mitochondria Takes place in liver and kidney Synthesis of glucose or glycogen from non carbohydrates like pyruvate, lactate glucogenic amino acids, glycerol and propionic acid Pathway involves steps of TCA cycle and reversal of glycolysis The irreversible steps o ...
File - western undergrad. by the students, for the students.
... generally accomplished by increasing the rate of recruitment of RNA polymerase or the activity of RNA polymerase. Both enhanced recruitment or activity of RNA polymerase are usually achieved by protein-protein interactions between the activator protein and RNA polymerase. The Lac Operon (Fig 8-9) Th ...
... generally accomplished by increasing the rate of recruitment of RNA polymerase or the activity of RNA polymerase. Both enhanced recruitment or activity of RNA polymerase are usually achieved by protein-protein interactions between the activator protein and RNA polymerase. The Lac Operon (Fig 8-9) Th ...
12 Interaction of Genes
... observed in crosses of two such mutations, when each affected a different functional domain. As expected, intragenic complementation of this type is often observed with missense mutations, not with deletions, and only with special subsets of nonsense mutations. Numerous examples of intragenic comple ...
... observed in crosses of two such mutations, when each affected a different functional domain. As expected, intragenic complementation of this type is often observed with missense mutations, not with deletions, and only with special subsets of nonsense mutations. Numerous examples of intragenic comple ...
Document
... by bacteria • May express antibiotic resistance gene or be modified to express proteins of interest ...
... by bacteria • May express antibiotic resistance gene or be modified to express proteins of interest ...
Evolution of gram-negative bacteria: a view from lipid A biosynthesis
... we examined. lpxH gene, which encodes pyrophosphatase, appeared to have arisen from a duplication of lpxH2 gene after beta/gammaproteobacteria were diverged from other proteobacteria. lpxH2 gene encodes phosphatase , and is not involved with lipid-A biosynthesis. Thus this new gene creation was foll ...
... we examined. lpxH gene, which encodes pyrophosphatase, appeared to have arisen from a duplication of lpxH2 gene after beta/gammaproteobacteria were diverged from other proteobacteria. lpxH2 gene encodes phosphatase , and is not involved with lipid-A biosynthesis. Thus this new gene creation was foll ...
Types of Agar
... • Selects for organisms of the genus Staphylococcus, since they can tolerate high saline levels. • contains the sugar mannitol and the pH indicator phenol red. If an organism can ferment mannitol, an acidic byproduct is formed that will cause the phenol red in the agar to turn yellow. • Most pathoge ...
... • Selects for organisms of the genus Staphylococcus, since they can tolerate high saline levels. • contains the sugar mannitol and the pH indicator phenol red. If an organism can ferment mannitol, an acidic byproduct is formed that will cause the phenol red in the agar to turn yellow. • Most pathoge ...
Proteins Synthesis
... machinery, which consists of ribosomes, ribosomal RNA (rRNA), and proteins that facilitate the addition of amino acids to form polypeptide NOTE: ****Prokaryotes and eukaryotes differ in how protein synthesis is organized within their cells. In Prokaryotes – happens in rapid successions (no nucleus ...
... machinery, which consists of ribosomes, ribosomal RNA (rRNA), and proteins that facilitate the addition of amino acids to form polypeptide NOTE: ****Prokaryotes and eukaryotes differ in how protein synthesis is organized within their cells. In Prokaryotes – happens in rapid successions (no nucleus ...
Current Microbiology
... During screening for new cry genes from B. thuringiensis strains isolated from plant leaves in Korea by DNA dot blot hybridization and PCR, we found a B. thuringiensis isolate, BR30, suggested to contain a new cry1Itype gene. The total DNA of this isolate responded positively to a cry1I-specific pro ...
... During screening for new cry genes from B. thuringiensis strains isolated from plant leaves in Korea by DNA dot blot hybridization and PCR, we found a B. thuringiensis isolate, BR30, suggested to contain a new cry1Itype gene. The total DNA of this isolate responded positively to a cry1I-specific pro ...
Lac operon
lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria. Although glucose is the preferred carbon source for most bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available. Gene regulation of the lac operon was the first genetic regulatory mechanism to be understood clearly, so it has become a foremost example of prokaryotic gene regulation. It is often discussed in introductory molecular and cellular biology classes at universities for this reason.Bacterial operons are polycistronic transcripts that are able to produce multiple proteins from one mRNA transcript. In this case, when lactose is required as a sugar source for the bacterium, the three genes of the lac operon can be expressed and their subsequent proteins translated: lacZ, lacY, and lacA. The gene product of lacZ is β-galactosidase which cleaves lactose, a disaccharide, into glucose and galactose. LacY encodes lactose permease, a protein which becomes embedded in the cytoplasmic membrane to enable transport of lactose into the cell. Finally, lacA encodes galactoside O-acetyltransferase. Layout of the lac operon.It would be wasteful to produce the enzymes when there is no lactose available or if there is a more preferable energy source available, such as glucose. The lac operon uses a two-part control mechanism to ensure that the cell expends energy producing the enzymes encoded by the lac operon only when necessary. In the absence of lactose, the lac repressor halts production of the enzymes encoded by the lac operon. In the presence of glucose, the catabolite activator protein (CAP), required for production of the enzymes, remains inactive, and EIIAGlc shuts down lactose permease to prevent transport of lactose into the cell. This dual control mechanism causes the sequential utilization of glucose and lactose in two distinct growth phases, known as diauxie.